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The map label for this gene is ygcM [C]

Identifier: 78357240

GI number: 78357240

Start: 2237993

End: 2238385

Strand: Direct

Name: ygcM [C]

Synonym: Dde_2197

Alternate gene names: 78357240

Gene position: 2237993-2238385 (Clockwise)

Preceding gene: 78357239

Following gene: 78357241

Centisome position: 60.0

GC content: 54.71

Gene sequence:

>393_bases
ATGCCTAAGGGAATATGGCGCCTTACCGTGCGTTCCGACTTTGCGGCGGCCCATTCGCTGCGCAACTATTGCGGCAAGTG
CGAAGCACTGCACGGCCATAACTTCGGCGTTGAAGCCACAGTGGAAGGGGGAATCCTCACGCCTGACACAGAGCTGCTGG
TGGATTTTAAAGTACTGAAAAACGCACTGAAACAGGTACTTGACGGTCTTGACCACTGCGTGCTTAACGAAACGCCCCCC
TTTGACACCTGCAATCCTTCGTCGGAAAATCTGGCAAGGCACATTTACCGGCATATGGCCCCCCTCGTACAACCGCACGG
GGTACGCATGCACAGTGTGACCGTGTCGGAAAAGCAGGCGCAATCGGCTACCTATATGGAAGTGGACGAATAA

Upstream 100 bases:

>100_bases
AGCTGGTGCCTGCTGCAGCTGGGGCCGGAATACTCTATCCAGCCCGGCCCCGTGTTTGATAACGACTTCAATCAGTGGAA
AATGAAAGGAAAAACTACTC

Downstream 100 bases:

>100_bases
TGGCAGACCTGTCTTTCCGCAGTGTTTTCGAGATAGCCCTGCCGCTGTTTCTGATTATGGACCCGCTGGGCAACGCCGCA
GTGTGCATCGGCATGCTGAA

Product: 6-pyruvoyl tetrahydrobiopterin synthase

Products: 6-pyruvoyl-5,6,7,8-tetrahydropterin; triphosphate

Alternate protein names: 6-Pyruvoyl-Tetrahydropterin Synthase; 6-Pyruvoyl Tetrahydrobiopterin Synthase; Queuosine Biosynthesis Protein QueD; 6-Pyruvoyltetrahydropterin Synthase; 6-Pyruvoyl Tetrahydrobiopterin Synthase Family Protein; Preq(0) Biosynthesis Protein Qued; 6-Carboxy-5 6 7 8-Tetrahydropterin Synthase; 6-Pyruvoyl Tetrahydropterin Synthase Family Protein; 6-Pyruvoyl Tetrahydrobiopterin Synthase-Like Protein; 6-Pyruvoyl Tetrahydropterin Synthase Superfamily; Queuosine Biosynthesis Protein; F0F1-Type ATP Synthase Subunit C

Number of amino acids: Translated: 130; Mature: 129

Protein sequence:

>130_residues
MPKGIWRLTVRSDFAAAHSLRNYCGKCEALHGHNFGVEATVEGGILTPDTELLVDFKVLKNALKQVLDGLDHCVLNETPP
FDTCNPSSENLARHIYRHMAPLVQPHGVRMHSVTVSEKQAQSATYMEVDE

Sequences:

>Translated_130_residues
MPKGIWRLTVRSDFAAAHSLRNYCGKCEALHGHNFGVEATVEGGILTPDTELLVDFKVLKNALKQVLDGLDHCVLNETPP
FDTCNPSSENLARHIYRHMAPLVQPHGVRMHSVTVSEKQAQSATYMEVDE
>Mature_129_residues
PKGIWRLTVRSDFAAAHSLRNYCGKCEALHGHNFGVEATVEGGILTPDTELLVDFKVLKNALKQVLDGLDHCVLNETPPF
DTCNPSSENLARHIYRHMAPLVQPHGVRMHSVTVSEKQAQSATYMEVDE

Specific function: Tetrahydrobiopterin biosynthesis; second step. [C]

COG id: COG0720

COG function: function code H; 6-pyruvoyl-tetrahydropterin synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 4.2.3.12

Molecular weight: Translated: 14454; Mature: 14322

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKGIWRLTVRSDFAAAHSLRNYCGKCEALHGHNFGVEATVEGGILTPDTELLVDFKVLK
CCCCEEEEEECHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCEECCCHHHHHHHHHHH
NALKQVLDGLDHCVLNETPPFDTCNPSSENLARHIYRHMAPLVQPHGVRMHSVTVSEKQA
HHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHH
QSATYMEVDE
CCCCEEEECC
>Mature Secondary Structure 
PKGIWRLTVRSDFAAAHSLRNYCGKCEALHGHNFGVEATVEGGILTPDTELLVDFKVLK
CCCEEEEEECHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCEECCCHHHHHHHHHHH
NALKQVLDGLDHCVLNETPPFDTCNPSSENLARHIYRHMAPLVQPHGVRMHSVTVSEKQA
HHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHH
QSATYMEVDE
CCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Mg2+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 17.7 {6-(L-erythro-1,2-dihydroxypropyl} 5 {6-(L-erythro-1,2-dihydroxypropyl} 0.0091 {6-(L-erythro-1,2-dihydroxypropyl} 0.008 {6-(L-erythro-1,2-dihydroxypropyl} [C]

Substrates: 6-[(1S,2R)-1,2-dihydroxy-3-triphosphooxypropyl]-7,8-dihydropterin

Specific reaction: 6-[(1S,2R)-1,2-dihydroxy-3-triphosphooxypropyl]-7,8-dihydropterin = 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate

General reaction: Intramolecular redox reaction; P-O bond cleavage; Triphosphate elimination [C]

Inhibitor: (NH4)2SO4 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA