Click here to switch to the map view.

The map label for this gene is cbiK [H]

Identifier: 78357224

GI number: 78357224

Start: 2221524

End: 2222375

Strand: Reverse

Name: cbiK [H]

Synonym: Dde_2181

Alternate gene names: 78357224

Gene position: 2222375-2221524 (Counterclockwise)

Preceding gene: 78357230

Following gene: 78357223

Centisome position: 59.58

GC content: 60.45

Gene sequence:

>852_bases
ATGAAACCGGGTATTCTTCTGGCCGCTTTCGGTGCCAGCAACCGTCAGGCGCATCAGACGCTCCGCCTGTTTGACGAAAA
GGTGCGGCAGCGGTTTGCCGATGTTCCTGTGCGCTGGGCCTTTACGTCGGGGCTTATCCGCGACCGGCTGGCGGCGCGCC
GCATGAAGACGGATTCCGTGACCAAAGCCCTGCAGAAGATGTGTTTTGAAAAATATACGCATGTGGCCGTGCAGTCGCTG
CATATCATTCCCGGTGCGGAATATGACGATTTGGAAGCCGAAGCTGTGCAGATGGCCGGCGGACGGGACTGCTCACTTGC
GCCGCCCGCTGACGGAGGCGTTTTTTTTACGGCTCAGGCGCGGTTTGAAAAGATAACCGTGGGCAGGCCCCTGCTGCACA
GCGATGCAGATGTGACCCGCGCCGCAAGGGCGCTGCTTGCCAGTCTGCCGCCGGAGCGCACGGCGCATGATGCCGTGGTG
TTCATGGGACACGGAACATGGCACGCCGGTGACAGCCGGTATGATGATCTTTCCGCTGCGGTGCGGCGTATGGATGCCGG
AGTGTTCATCGGCACCATGGACGGCAGCCACACCATCGACCATGTGCTGCCGCTTCTGCTTCAGGAAGGATACTGTCGTG
TGTTTCTTCAGCCTTTACTGTCTGTTGTGGGGCGTCATGCCGTGCGGGACATGGCAGGCGATCATCCCGAATCATGGCGT
TCGCGCATAGCCGCTGCGGGCATGGTGTGCCTGCCTGTGCTGCAGGGCATGGTCGAGCATGCGGCTTTTGTGGATATATG
GATAGATCATCTGGATGAAGCCATGGAGCGGCTGCTCGGCAGCCGCGGCTGA

Upstream 100 bases:

>100_bases
AGGCTTGCCAACTCTTCGGCCCTGTCGTTACCATTTCCGGCCTGTAAATAATCTCCCTCCGTTTCAGGAGGTCTGCTCAT
CAGGTAGGGTGTGAAATCAT

Downstream 100 bases:

>100_bases
CGGGAAGGCGAGGGGAGGAATGCATGATCTTGACATCTGTGCGCCGCGGTCTGGCTGTGTGCCTGTTTCTGTTGCTGGTG
CCTCTGGCCGCCGGTGCTGC

Product: heme-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MKPGILLAAFGASNRQAHQTLRLFDEKVRQRFADVPVRWAFTSGLIRDRLAARRMKTDSVTKALQKMCFEKYTHVAVQSL
HIIPGAEYDDLEAEAVQMAGGRDCSLAPPADGGVFFTAQARFEKITVGRPLLHSDADVTRAARALLASLPPERTAHDAVV
FMGHGTWHAGDSRYDDLSAAVRRMDAGVFIGTMDGSHTIDHVLPLLLQEGYCRVFLQPLLSVVGRHAVRDMAGDHPESWR
SRIAAAGMVCLPVLQGMVEHAAFVDIWIDHLDEAMERLLGSRG

Sequences:

>Translated_283_residues
MKPGILLAAFGASNRQAHQTLRLFDEKVRQRFADVPVRWAFTSGLIRDRLAARRMKTDSVTKALQKMCFEKYTHVAVQSL
HIIPGAEYDDLEAEAVQMAGGRDCSLAPPADGGVFFTAQARFEKITVGRPLLHSDADVTRAARALLASLPPERTAHDAVV
FMGHGTWHAGDSRYDDLSAAVRRMDAGVFIGTMDGSHTIDHVLPLLLQEGYCRVFLQPLLSVVGRHAVRDMAGDHPESWR
SRIAAAGMVCLPVLQGMVEHAAFVDIWIDHLDEAMERLLGSRG
>Mature_283_residues
MKPGILLAAFGASNRQAHQTLRLFDEKVRQRFADVPVRWAFTSGLIRDRLAARRMKTDSVTKALQKMCFEKYTHVAVQSL
HIIPGAEYDDLEAEAVQMAGGRDCSLAPPADGGVFFTAQARFEKITVGRPLLHSDADVTRAARALLASLPPERTAHDAVV
FMGHGTWHAGDSRYDDLSAAVRRMDAGVFIGTMDGSHTIDHVLPLLLQEGYCRVFLQPLLSVVGRHAVRDMAGDHPESWR
SRIAAAGMVCLPVLQGMVEHAAFVDIWIDHLDEAMERLLGSRG

Specific function: Chelates cobalt to the cobalamin precursor as part of the anaerobic pathway to cobalamin biosynthesis [H]

COG id: COG4822

COG function: function code H; Cobalamin biosynthesis protein CbiK, Co2+ chelatase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CbiK family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010388 [H]

Pfam domain/function: PF06180 CbiK [H]

EC number: =4.99.1.3 [H]

Molecular weight: Translated: 31162; Mature: 31162

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPGILLAAFGASNRQAHQTLRLFDEKVRQRFADVPVRWAFTSGLIRDRLAARRMKTDSV
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
TKALQKMCFEKYTHVAVQSLHIIPGAEYDDLEAEAVQMAGGRDCSLAPPADGGVFFTAQA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCEEEEECC
RFEKITVGRPLLHSDADVTRAARALLASLPPERTAHDAVVFMGHGTWHAGDSRYDDLSAA
CHHEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHH
VRRMDAGVFIGTMDGSHTIDHVLPLLLQEGYCRVFLQPLLSVVGRHAVRDMAGDHPESWR
HHHHCCCEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
SRIAAAGMVCLPVLQGMVEHAAFVDIWIDHLDEAMERLLGSRG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKPGILLAAFGASNRQAHQTLRLFDEKVRQRFADVPVRWAFTSGLIRDRLAARRMKTDSV
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
TKALQKMCFEKYTHVAVQSLHIIPGAEYDDLEAEAVQMAGGRDCSLAPPADGGVFFTAQA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCEEEEECC
RFEKITVGRPLLHSDADVTRAARALLASLPPERTAHDAVVFMGHGTWHAGDSRYDDLSAA
CHHEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHH
VRRMDAGVFIGTMDGSHTIDHVLPLLLQEGYCRVFLQPLLSVVGRHAVRDMAGDHPESWR
HHHHCCCEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
SRIAAAGMVCLPVLQGMVEHAAFVDIWIDHLDEAMERLLGSRG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501034; 11677609; 9150215; 10451360 [H]