| Definition | Desulfovibrio desulfuricans subsp. desulfuricans str. G20 chromosome, complete genome. |
|---|---|
| Accession | NC_007519 |
| Length | 3,730,232 |
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The map label for this gene is metH [H]
Identifier: 78357158
GI number: 78357158
Start: 2162903
End: 2165428
Strand: Reverse
Name: metH [H]
Synonym: Dde_2115
Alternate gene names: 78357158
Gene position: 2165428-2162903 (Counterclockwise)
Preceding gene: 78357174
Following gene: 78357157
Centisome position: 58.05
GC content: 59.18
Gene sequence:
>2526_bases ATGTTTTTCAGTAGACCGAAGCGGTTCATACTGCCAAACCGCGGCGGGACGCGCCGTTTTTTTACCAAAAGTTTCTGCGA GGAGATAGCCGCCGTGCCCGATTTCAGACGAGCGCTGAGTGATGATACCCTGTTGTTTTTCGACGGGGCCATGGGAACCA TGCTGCAGGGGCGGGGGCTGCCCGCCGGCATGAGTCCCGAAGTCTTCTGTCTTTCCAATCCGGCTGTGCTGCAGGGTGTT CATCTGGACTACGCACGCGCAGGGGCGGATGTGCTGACCACCAACACGTTCGGGGGAACACGCCTCAAGCTGCCCGAGGG CATGAACGTGGTGGAATTCAACCGCGAAATGGCCAGAGCCGCAAAGGCCGCCGCCGGTCAGGCCGGACGCACCGTGTTTG TGGCGGGCAGTGTGGGGCCCACAGGACACTTTGTAAAGCCGCTCGGCGATCTGGAATTTTCGGAACTGGTTGATATTTTT CGTGAACAGATCCGCGGGCTTGTGCAGGGCGGAATAGACCTTGTGCTTGCCGAAACGCATTTTGATCTTGCCGAAGTGCG TGCCGTGGTGCTGGCCGCGCGCGCCGAGTGCAATCTGCCTGTGGGCGTGTCCATGACCTTTGAAGATGGCGTGAGTCTTA CAGGAACACGTCCCGAAGTTTTTGTCCATACCATGCAGAACATGGGAGTGGACCTTATTGCCACCAACTGCAGCGCAGGA CCGGAGCAGATGGCGGGAGTGGTGGCCGGACTGACTGCCGCGGGAGACATACCCGTACTGGTCATGCCCAATGCCGGCCT GCCCGAGCTGGAAAACGGCAACACGGTGTTCCGGCTGGGACCGGAAGAGTTTGCCGCGCAGACTGCGGACTTTGTGCGTG CCGGTGTGCGGTGTCTCGGCGGGTGCTGCGGTACGACCCCCGATCATATTGCCGCGCTGGCGCGTGAGGCGCGCAGCAAG CCCCGTCCGGCGGTTGAAAAACGCCGCCGCTCCGGCATTGTGCTGACGACCAGAGCCGCTTGTCTGCGTATCGGTCACGA CCAGCCCTGCAGGATAATCGGGGAACGTATAAACCCTACCGGCAAAAAGGAACTGACAGCACAGCTGCAGGCAGGCGATT TTACACTGGCCATGCAGTTTGCACAGCAGCAGATTGATGCCGGAGCCCCCTTGCTGGATGTGAACGTGGGTGCGCCCATG GTGGACGAAAAAGTGCTGCTGCCGCAGCTGATCTCTGACCTCGCTGCAGCTCACGGGGTTCCTCTTTCCATAGACAGCTC GGATATGGACGCCGTGGAGGCTTCGCTTGCCGTATACGCGGGGTCCCCTCTGGTCAATTCCATCAGCGGCGAACCGGGAC GCATGGAACGCCTGGGGCCGCTGTGCAGAGATTACGGGGCGCCATTTGTTCTGCTGCCCCTGAAAGGCCGAAAACTGCCC GTCACGGCCGCCGAGCGTATATCCATCATCGATGAACTGCTTCTGCAGGCCGATGATCTGGGCATTCCCCGCAGGCTGGT TATGGTTGATGTGCTGGCTCTGGCTGTTTCCTCCAAGGCGGAAGCTGCGCGGCACTGTTTCGATACCATCCGGTATTGCA ACGAAGAGCTGGGACTGCCCACCACCATTGGACTGTCAAATATTTCTTTCGGTCTGCCTGCGCGTGAACTGCTGAATGCC ACGTTTCTTGCCATGGCCGTGGGGGCCGGACTTACTTCCTGTATCGCCCACCCCGGCAACGTCAGACTGCGGGAAGCACT GGCCGCAGCCGAGGTGCTTATGGCGCGCGACAGCAATGCCGAGCGGTTTATCGCCGGCTACTCCGGCTGGACATCAGGAG GCGGCAGCGGCGGCATTATTGCCGGCGGCGGAGAAGAGAAAAAACGCCCCGTCAGTCTGGAAGAGGCTGTCATACGCGGA GAACGCGACATCATTCTGGAACTGGTGGAAGACGAACTGGCCCGAGGCGCACAACCGTATGCTCTTGTTAATGAAAGGTT GATTCCGGCCATTACCGAGGTGGGCGACAAATACGAGCGCAAGGAGTATTACCTGCCGCAGCTGCTGCGTTCCGCAGAAA CCATGCAGAAAGCTTTCGGCAGGCTTAAGCCCCTGCTGGAAGAGGCTTCCGGCGGCGAGACAAAACCGGTCATCATTATG GCCACCGTGGAAGGCGATATTCATGACATCGGAAAGAATATTGTCTGTCTTATGCTGGGCAACCATGGTTTTGATGTTGT TGATCTGGGCAAAGATGTTAAAGCGGTGGATATTGTAGATGCCGCAGTGGAGCATAACGCGAAGCTGATAGGGCTTTCGG CCCTGATGACCACTACCATGGTGCGCATGGAAGACACCATACAGCTGCTGCGCGAACGCGGGCTTTCCATTCCTGTTTTT GTGGGCGGGGCTGTTGTCACGCCTGCTTTTGCCGAAGCCATCGGCGCAGCGGGATACTCCCGCGATGCCGTGGAAGCGGT GAGAATTGCGCGGGGGCTTGTGGAAGCTCCAGCCAGAGTGCAGTGA
Upstream 100 bases:
>100_bases GCTTTAACCGGCATCAGCCGGATATGATCTTTGATGGGAAGTCCTGCTCTTAAGAACAGGCTTGGGCATTGAATGGTATA GTTTTAGTTTGTCCTTTTCA
Downstream 100 bases:
>100_bases GGAGTTACAGATATGAGACGTTCTTTTTTGATCATGCGGACGATGCTGACGGTGTGCTGCCTTGTTGTCCTGTGGGCCGG AGCTGTCCGCGCCGCGGATG
Product: methionine synthase
Products: NA
Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]
Number of amino acids: Translated: 841; Mature: 841
Protein sequence:
>841_residues MFFSRPKRFILPNRGGTRRFFTKSFCEEIAAVPDFRRALSDDTLLFFDGAMGTMLQGRGLPAGMSPEVFCLSNPAVLQGV HLDYARAGADVLTTNTFGGTRLKLPEGMNVVEFNREMARAAKAAAGQAGRTVFVAGSVGPTGHFVKPLGDLEFSELVDIF REQIRGLVQGGIDLVLAETHFDLAEVRAVVLAARAECNLPVGVSMTFEDGVSLTGTRPEVFVHTMQNMGVDLIATNCSAG PEQMAGVVAGLTAAGDIPVLVMPNAGLPELENGNTVFRLGPEEFAAQTADFVRAGVRCLGGCCGTTPDHIAALAREARSK PRPAVEKRRRSGIVLTTRAACLRIGHDQPCRIIGERINPTGKKELTAQLQAGDFTLAMQFAQQQIDAGAPLLDVNVGAPM VDEKVLLPQLISDLAAAHGVPLSIDSSDMDAVEASLAVYAGSPLVNSISGEPGRMERLGPLCRDYGAPFVLLPLKGRKLP VTAAERISIIDELLLQADDLGIPRRLVMVDVLALAVSSKAEAARHCFDTIRYCNEELGLPTTIGLSNISFGLPARELLNA TFLAMAVGAGLTSCIAHPGNVRLREALAAAEVLMARDSNAERFIAGYSGWTSGGGSGGIIAGGGEEKKRPVSLEEAVIRG ERDIILELVEDELARGAQPYALVNERLIPAITEVGDKYERKEYYLPQLLRSAETMQKAFGRLKPLLEEASGGETKPVIIM ATVEGDIHDIGKNIVCLMLGNHGFDVVDLGKDVKAVDIVDAAVEHNAKLIGLSALMTTTMVRMEDTIQLLRERGLSIPVF VGGAVVTPAFAEAIGAAGYSRDAVEAVRIARGLVEAPARVQ
Sequences:
>Translated_841_residues MFFSRPKRFILPNRGGTRRFFTKSFCEEIAAVPDFRRALSDDTLLFFDGAMGTMLQGRGLPAGMSPEVFCLSNPAVLQGV HLDYARAGADVLTTNTFGGTRLKLPEGMNVVEFNREMARAAKAAAGQAGRTVFVAGSVGPTGHFVKPLGDLEFSELVDIF REQIRGLVQGGIDLVLAETHFDLAEVRAVVLAARAECNLPVGVSMTFEDGVSLTGTRPEVFVHTMQNMGVDLIATNCSAG PEQMAGVVAGLTAAGDIPVLVMPNAGLPELENGNTVFRLGPEEFAAQTADFVRAGVRCLGGCCGTTPDHIAALAREARSK PRPAVEKRRRSGIVLTTRAACLRIGHDQPCRIIGERINPTGKKELTAQLQAGDFTLAMQFAQQQIDAGAPLLDVNVGAPM VDEKVLLPQLISDLAAAHGVPLSIDSSDMDAVEASLAVYAGSPLVNSISGEPGRMERLGPLCRDYGAPFVLLPLKGRKLP VTAAERISIIDELLLQADDLGIPRRLVMVDVLALAVSSKAEAARHCFDTIRYCNEELGLPTTIGLSNISFGLPARELLNA TFLAMAVGAGLTSCIAHPGNVRLREALAAAEVLMARDSNAERFIAGYSGWTSGGGSGGIIAGGGEEKKRPVSLEEAVIRG ERDIILELVEDELARGAQPYALVNERLIPAITEVGDKYERKEYYLPQLLRSAETMQKAFGRLKPLLEEASGGETKPVIIM ATVEGDIHDIGKNIVCLMLGNHGFDVVDLGKDVKAVDIVDAAVEHNAKLIGLSALMTTTMVRMEDTIQLLRERGLSIPVF VGGAVVTPAFAEAIGAAGYSRDAVEAVRIARGLVEAPARVQ >Mature_841_residues MFFSRPKRFILPNRGGTRRFFTKSFCEEIAAVPDFRRALSDDTLLFFDGAMGTMLQGRGLPAGMSPEVFCLSNPAVLQGV HLDYARAGADVLTTNTFGGTRLKLPEGMNVVEFNREMARAAKAAAGQAGRTVFVAGSVGPTGHFVKPLGDLEFSELVDIF REQIRGLVQGGIDLVLAETHFDLAEVRAVVLAARAECNLPVGVSMTFEDGVSLTGTRPEVFVHTMQNMGVDLIATNCSAG PEQMAGVVAGLTAAGDIPVLVMPNAGLPELENGNTVFRLGPEEFAAQTADFVRAGVRCLGGCCGTTPDHIAALAREARSK PRPAVEKRRRSGIVLTTRAACLRIGHDQPCRIIGERINPTGKKELTAQLQAGDFTLAMQFAQQQIDAGAPLLDVNVGAPM VDEKVLLPQLISDLAAAHGVPLSIDSSDMDAVEASLAVYAGSPLVNSISGEPGRMERLGPLCRDYGAPFVLLPLKGRKLP VTAAERISIIDELLLQADDLGIPRRLVMVDVLALAVSSKAEAARHCFDTIRYCNEELGLPTTIGLSNISFGLPARELLNA TFLAMAVGAGLTSCIAHPGNVRLREALAAAEVLMARDSNAERFIAGYSGWTSGGGSGGIIAGGGEEKKRPVSLEEAVIRG ERDIILELVEDELARGAQPYALVNERLIPAITEVGDKYERKEYYLPQLLRSAETMQKAFGRLKPLLEEASGGETKPVIIM ATVEGDIHDIGKNIVCLMLGNHGFDVVDLGKDVKAVDIVDAAVEHNAKLIGLSALMTTTMVRMEDTIQLLRERGLSIPVF VGGAVVTPAFAEAIGAAGYSRDAVEAVRIARGLVEAPARVQ
Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]
COG id: COG1410
COG function: function code E; Methionine synthase I, cobalamin-binding domain
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 pterin-binding domain [H]
Homologues:
Organism=Homo sapiens, GI169790923, Length=899, Percent_Identity=29.6996662958843, Blast_Score=297, Evalue=2e-80, Organism=Escherichia coli, GI1790450, Length=852, Percent_Identity=29.9295774647887, Blast_Score=325, Evalue=1e-89, Organism=Escherichia coli, GI1786456, Length=325, Percent_Identity=31.6923076923077, Blast_Score=100, Evalue=4e-22, Organism=Caenorhabditis elegans, GI17535405, Length=892, Percent_Identity=28.0269058295964, Blast_Score=291, Evalue=9e-79,
Paralogues:
None
Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR006158 - InterPro: IPR011005 - InterPro: IPR003759 - InterPro: IPR011822 - InterPro: IPR000489 - InterPro: IPR003726 - InterPro: IPR004223 [H]
Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]
EC number: =2.1.1.13 [H]
Molecular weight: Translated: 89515; Mature: 89515
Theoretical pI: Translated: 5.19; Mature: 5.19
Prosite motif: PS50970 HCY ; PS50972 PTERIN_BINDING
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.7 %Cys (Translated Protein) 2.9 %Met (Translated Protein) 4.5 %Cys+Met (Translated Protein) 1.7 %Cys (Mature Protein) 2.9 %Met (Mature Protein) 4.5 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MFFSRPKRFILPNRGGTRRFFTKSFCEEIAAVPDFRRALSDDTLLFFDGAMGTMLQGRGL CCCCCCCCEECCCCCCCHHHHHHHHHHHHHCCCHHHHHCCCCEEEEECCCHHHHHCCCCC PAGMSPEVFCLSNPAVLQGVHLDYARAGADVLTTNTFGGTRLKLPEGMNVVEFNREMARA CCCCCCCEEEECCCHHHEECCHHHHHCCCCEEEECCCCCEEEECCCCCCHHHHHHHHHHH AKAAAGQAGRTVFVAGSVGPTGHFVKPLGDLEFSELVDIFREQIRGLVQGGIDLVLAETH HHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECC FDLAEVRAVVLAARAECNLPVGVSMTFEDGVSLTGTRPEVFVHTMQNMGVDLIATNCSAG CCHHHHHHHHHHHHCCCCCCCEEEEECCCCCEECCCCHHHHHHHHHHCCEEEEEECCCCC PEQMAGVVAGLTAAGDIPVLVMPNAGLPELENGNTVFRLGPEEFAAQTADFVRAGVRCLG HHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHH GCCGTTPDHIAALAREARSKPRPAVEKRRRSGIVLTTRAACLRIGHDQPCRIIGERINPT CCCCCCHHHHHHHHHHHHCCCCCHHHHHHHCCEEEEEHHHHHHCCCCCCHHHHHCCCCCC GKKELTAQLQAGDFTLAMQFAQQQIDAGAPLLDVNVGAPMVDEKVLLPQLISDLAAAHGV CCHHHHEEEECCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCC PLSIDSSDMDAVEASLAVYAGSPLVNSISGEPGRMERLGPLCRDYGAPFVLLPLKGRKLP CEEECCCCHHHHHHHHHEEECCCHHHCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCC VTAAERISIIDELLLQADDLGIPRRLVMVDVLALAVSSKAEAARHCFDTIRYCNEELGLP CCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCC TTIGLSNISFGLPARELLNATFLAMAVGAGLTSCIAHPGNVRLREALAAAEVLMARDSNA EEECCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHCCCCC ERFIAGYSGWTSGGGSGGIIAGGGEEKKRPVSLEEAVIRGERDIILELVEDELARGAQPY CEEEEECCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHCCCCCH ALVNERLIPAITEVGDKYERKEYYLPQLLRSAETMQKAFGRLKPLLEEASGGETKPVIIM HHHHCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEE ATVEGDIHDIGKNIVCLMLGNHGFDVVDLGKDVKAVDIVDAAVEHNAKLIGLSALMTTTM EEECCCHHHCCCCEEEEEECCCCCCEEECCCCCCHHHHHHHHHHCCCEEEEHHHHHHHHH VRMEDTIQLLRERGLSIPVFVGGAVVTPAFAEAIGAAGYSRDAVEAVRIARGLVEAPARV HHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCC Q C >Mature Secondary Structure MFFSRPKRFILPNRGGTRRFFTKSFCEEIAAVPDFRRALSDDTLLFFDGAMGTMLQGRGL CCCCCCCCEECCCCCCCHHHHHHHHHHHHHCCCHHHHHCCCCEEEEECCCHHHHHCCCCC PAGMSPEVFCLSNPAVLQGVHLDYARAGADVLTTNTFGGTRLKLPEGMNVVEFNREMARA CCCCCCCEEEECCCHHHEECCHHHHHCCCCEEEECCCCCEEEECCCCCCHHHHHHHHHHH AKAAAGQAGRTVFVAGSVGPTGHFVKPLGDLEFSELVDIFREQIRGLVQGGIDLVLAETH HHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECC FDLAEVRAVVLAARAECNLPVGVSMTFEDGVSLTGTRPEVFVHTMQNMGVDLIATNCSAG CCHHHHHHHHHHHHCCCCCCCEEEEECCCCCEECCCCHHHHHHHHHHCCEEEEEECCCCC PEQMAGVVAGLTAAGDIPVLVMPNAGLPELENGNTVFRLGPEEFAAQTADFVRAGVRCLG HHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHH GCCGTTPDHIAALAREARSKPRPAVEKRRRSGIVLTTRAACLRIGHDQPCRIIGERINPT CCCCCCHHHHHHHHHHHHCCCCCHHHHHHHCCEEEEEHHHHHHCCCCCCHHHHHCCCCCC GKKELTAQLQAGDFTLAMQFAQQQIDAGAPLLDVNVGAPMVDEKVLLPQLISDLAAAHGV CCHHHHEEEECCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCC PLSIDSSDMDAVEASLAVYAGSPLVNSISGEPGRMERLGPLCRDYGAPFVLLPLKGRKLP CEEECCCCHHHHHHHHHEEECCCHHHCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCC VTAAERISIIDELLLQADDLGIPRRLVMVDVLALAVSSKAEAARHCFDTIRYCNEELGLP CCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCC TTIGLSNISFGLPARELLNATFLAMAVGAGLTSCIAHPGNVRLREALAAAEVLMARDSNA EEECCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHCCCCC ERFIAGYSGWTSGGGSGGIIAGGGEEKKRPVSLEEAVIRGERDIILELVEDELARGAQPY CEEEEECCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHCCCCCH ALVNERLIPAITEVGDKYERKEYYLPQLLRSAETMQKAFGRLKPLLEEASGGETKPVIIM HHHHCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEE ATVEGDIHDIGKNIVCLMLGNHGFDVVDLGKDVKAVDIVDAAVEHNAKLIGLSALMTTTM EEECCCHHHCCCCEEEEEECCCCCCEEECCCCCCHHHHHHHHHHCCCEEEEHHHHHHHHH VRMEDTIQLLRERGLSIPVFVGGAVVTPAFAEAIGAAGYSRDAVEAVRIARGLVEAPARV HHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCC Q C
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 10984043 [H]