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The map label for this gene is gltD [H]

Identifier: 78357129

GI number: 78357129

Start: 2133328

End: 2134386

Strand: Reverse

Name: gltD [H]

Synonym: Dde_2086

Alternate gene names: 78357129

Gene position: 2134386-2133328 (Counterclockwise)

Preceding gene: 78357130

Following gene: 78357128

Centisome position: 57.22

GC content: 56.85

Gene sequence:

>1059_bases
ATGTCCAATCCCATGAATTTTGCTTTTTTAAGAAAATCCCCGCTACCGCCCAACGGCCGCAAAGTGGGCATTATAGGTGC
AGGCCCCTCCGGACTTGCCGCCTGCGGATATCTGGCCTGTCTGGGATACGAGGTTGTGGTGTACGACAAACTGCCGCAAC
CCGGAGGATTGATGGTGTTCGGCATTCCCAGAAAGCGTATTCCGCCTGAACGTATCCGGGCAGGTGTGCGGCGCATGGAA
CGACAGTTCGGAGTTGTGTTCCGCACAAAGACAAAAATATGCTGCAGTGCTCCGTTGCATGAAGAGGAAGGCGATCACTT
TTCCTGTGATATCCGCGGCCTCGGCGAGCTTGTGGAAGAGCATGATGCGGTCATGATCTGTACCGGTTCATGGAAGTCCC
GAAAGCTTGGTATTCCCGGAGAAAATCTGGCGGGAGTGCATTCCGGCCTTGAGTTTTTGTTTCCCATCAGGGCGGCAAAA
TATGCCTCGCCCAATGTCACCATGCCCGATGTGCGCGGAAAGCGCGTGGCTGTGGTCGGCGCCGGTCATTCCGCCGTTGA
CGTGGTGGACAGTGCCCATGCGCTTGGTGCCGCCAAAATATACATGATATACAGACGGACAGCCCGCGAGGCCCCCTGCG
GCAGTTTCGAGATTGACCGCATCTGCGAGATGGGCGCCGAATGGCTTGAGCGGCGCACACCGCTGCGGGTGGAAGGTGAA
TGCGGCGTGCAGCGGCTGGAGCTTGCCAACGGTATGACCGGCGAGGCGGAGCATCTGGATGTGGACATGCTTATAACTGC
CATCGGTGAAATTCCCACTCCTCCCTTTCAGCAGGAACTGGGGCTAGAAAACGTACGCAAAGGCGAAGTGCGCTGGCTTC
ATATGACCAGTATAGAGAACGTGTTTGTCGCCGGAGATGTGCTGACAGGTCCCAGCAAGATAGGCAAGGCTGTCTACAGC
GGTTTGCGGGCTTCGCGATCGCTGACCAACTGGCTTGACCTGAAGGCGCAGAACCGCGAGGGCGAATACAGATACGACGA
GGACAGAATTGTCCCCTAG

Upstream 100 bases:

>100_bases
AGAGCACACGGCGCGGGGTTGCGCATGCCCGCTGCTTGGATATACTGTATGGCCGATCAGTCGTTTTGACCCATGATAAA
CGGCGCAGTACGGAGAATAT

Downstream 100 bases:

>100_bases
CCAGCATAGCTCGGAGGCTCAATGTAATGTCCTGTGAAGGGAAAACTCTTTTTATTGATTACAGCCGCTGTATCGGCTGT
GAAAGTTGCGAGGCTGTCTG

Product: putative glutamate synthase subunit beta

Products: NA

Alternate protein names: Glutamate synthase subunit beta; GLTS beta chain; NADPH-GOGAT [H]

Number of amino acids: Translated: 352; Mature: 351

Protein sequence:

>352_residues
MSNPMNFAFLRKSPLPPNGRKVGIIGAGPSGLAACGYLACLGYEVVVYDKLPQPGGLMVFGIPRKRIPPERIRAGVRRME
RQFGVVFRTKTKICCSAPLHEEEGDHFSCDIRGLGELVEEHDAVMICTGSWKSRKLGIPGENLAGVHSGLEFLFPIRAAK
YASPNVTMPDVRGKRVAVVGAGHSAVDVVDSAHALGAAKIYMIYRRTAREAPCGSFEIDRICEMGAEWLERRTPLRVEGE
CGVQRLELANGMTGEAEHLDVDMLITAIGEIPTPPFQQELGLENVRKGEVRWLHMTSIENVFVAGDVLTGPSKIGKAVYS
GLRASRSLTNWLDLKAQNREGEYRYDEDRIVP

Sequences:

>Translated_352_residues
MSNPMNFAFLRKSPLPPNGRKVGIIGAGPSGLAACGYLACLGYEVVVYDKLPQPGGLMVFGIPRKRIPPERIRAGVRRME
RQFGVVFRTKTKICCSAPLHEEEGDHFSCDIRGLGELVEEHDAVMICTGSWKSRKLGIPGENLAGVHSGLEFLFPIRAAK
YASPNVTMPDVRGKRVAVVGAGHSAVDVVDSAHALGAAKIYMIYRRTAREAPCGSFEIDRICEMGAEWLERRTPLRVEGE
CGVQRLELANGMTGEAEHLDVDMLITAIGEIPTPPFQQELGLENVRKGEVRWLHMTSIENVFVAGDVLTGPSKIGKAVYS
GLRASRSLTNWLDLKAQNREGEYRYDEDRIVP
>Mature_351_residues
SNPMNFAFLRKSPLPPNGRKVGIIGAGPSGLAACGYLACLGYEVVVYDKLPQPGGLMVFGIPRKRIPPERIRAGVRRMER
QFGVVFRTKTKICCSAPLHEEEGDHFSCDIRGLGELVEEHDAVMICTGSWKSRKLGIPGENLAGVHSGLEFLFPIRAAKY
ASPNVTMPDVRGKRVAVVGAGHSAVDVVDSAHALGAAKIYMIYRRTAREAPCGSFEIDRICEMGAEWLERRTPLRVEGEC
GVQRLELANGMTGEAEHLDVDMLITAIGEIPTPPFQQELGLENVRKGEVRWLHMTSIENVFVAGDVLTGPSKIGKAVYSG
LRASRSLTNWLDLKAQNREGEYRYDEDRIVP

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Escherichia coli, GI1789606, Length=352, Percent_Identity=27.5568181818182, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1788811, Length=346, Percent_Identity=28.3236994219653, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI87082180, Length=354, Percent_Identity=27.1186440677966, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1788468, Length=314, Percent_Identity=27.3885350318471, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1789243, Length=322, Percent_Identity=26.3975155279503, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17570289, Length=203, Percent_Identity=31.0344827586207, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17543860, Length=171, Percent_Identity=30.9941520467836, Blast_Score=76, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6320030, Length=191, Percent_Identity=29.8429319371728, Blast_Score=77, Evalue=3e-15,
Organism=Drosophila melanogaster, GI28574881, Length=350, Percent_Identity=25.4285714285714, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24665539, Length=350, Percent_Identity=25.4285714285714, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24665547, Length=350, Percent_Identity=25.4285714285714, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24665543, Length=350, Percent_Identity=25.4285714285714, Blast_Score=102, Evalue=5e-22,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006
- InterPro:   IPR009051
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 38672; Mature: 38541

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNPMNFAFLRKSPLPPNGRKVGIIGAGPSGLAACGYLACLGYEVVVYDKLPQPGGLMVF
CCCCCCEEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCEEEEEECCCCCCCEEEE
GIPRKRIPPERIRAGVRRMERQFGVVFRTKTKICCSAPLHEEEGDHFSCDIRGLGELVEE
CCCCCCCCHHHHHHHHHHHHHHHCEEEEECCCEEECCCCCCCCCCEEEECHHHHHHHHHH
HDAVMICTGSWKSRKLGIPGENLAGVHSGLEFLFPIRAAKYASPNVTMPDVRGKRVAVVG
CCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEE
AGHSAVDVVDSAHALGAAKIYMIYRRTAREAPCGSFEIDRICEMGAEWLERRTPLRVEGE
CCCHHHHHHHHHHHHHHHHEEEEEHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCC
CGVQRLELANGMTGEAEHLDVDMLITAIGEIPTPPFQQELGLENVRKGEVRWLHMTSIEN
CCCHHHHHHCCCCCCHHHCHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCEEEEEECCCCC
VFVAGDVLTGPSKIGKAVYSGLRASRSLTNWLDLKAQNREGEYRYDEDRIVP
EEEECCEECCHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SNPMNFAFLRKSPLPPNGRKVGIIGAGPSGLAACGYLACLGYEVVVYDKLPQPGGLMVF
CCCCCEEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCEEEEEECCCCCCCEEEE
GIPRKRIPPERIRAGVRRMERQFGVVFRTKTKICCSAPLHEEEGDHFSCDIRGLGELVEE
CCCCCCCCHHHHHHHHHHHHHHHCEEEEECCCEEECCCCCCCCCCEEEECHHHHHHHHHH
HDAVMICTGSWKSRKLGIPGENLAGVHSGLEFLFPIRAAKYASPNVTMPDVRGKRVAVVG
CCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEE
AGHSAVDVVDSAHALGAAKIYMIYRRTAREAPCGSFEIDRICEMGAEWLERRTPLRVEGE
CCCHHHHHHHHHHHHHHHHEEEEEHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCC
CGVQRLELANGMTGEAEHLDVDMLITAIGEIPTPPFQQELGLENVRKGEVRWLHMTSIEN
CCCHHHHHHCCCCCCHHHCHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCEEEEEECCCCC
VFVAGDVLTGPSKIGKAVYSGLRASRSLTNWLDLKAQNREGEYRYDEDRIVP
EEEECCEECCHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3326786; 9278503; 9298646; 9600841; 2643092 [H]