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The map label for this gene is pcm

Identifier: 78357120

GI number: 78357120

Start: 2118551

End: 2119192

Strand: Reverse

Name: pcm

Synonym: Dde_2077

Alternate gene names: 78357120

Gene position: 2119192-2118551 (Counterclockwise)

Preceding gene: 78357123

Following gene: 78357119

Centisome position: 56.81

GC content: 59.19

Gene sequence:

>642_bases
ATGATTGATAAAAGGCGCAGCAGAGAACGTATGGTGCGCGAGCAGCTAACAGCGAGGGGAATAACAGACCCCGCCGTGCT
GGCCGCCATGCGCAAGATACCCAGACATCTTTTTGTTCAGGAGGCTCTGCAGGCGCAGGCCTATGAGGACCATCCGCTTC
CCATAGGCTATGGTCAGACCATCTCGCAGCCGTTCATCGTGGCGCTGATGTCACAGATTCTGCGAGTCACTCCGGGAATG
CGTGTGCTTGAAATAGGGACAGGGTCCGGCTATCAGGCGGCCGTGCTGGCTGAAATGGGAGCCGAAGTCTATACCGTGGA
AAGAATTGCGGGACTGCAGGCCCATGCACGCGGCCTGCTGCGCAGGCTGGGATACGCCAGAATACGGACAAAGCTGGATG
ACGGCACCATGGGCTGGCCGCTGGCAGCCCCTTTTGACAGAATCATCGTCACGGCCGGAGGGCCCGGCATTCCCGAGCCG
CTGGCGGAACAGCTGGCAGACCCCGGCACCATGGCCATACCTGTGGGCGCTTCGCGGCGCGAACAGGAGCTTTATTTGAT
GCATAAGAACGACGGCGCATTGTCGTATGAAAATTATGGCAAGGTGGCCTTTGTGGACCTTGTCGGCAACCACGGGTGGT
AG

Upstream 100 bases:

>100_bases
CAAGGTTGACTTAGAGGCGAAAATCTGATTTCCAACCGAAGCCCCGCCTGTTATTGTCGGCGGCACAGGCCACAACGGGC
TCGAAGGAAAGCCACACGCT

Downstream 100 bases:

>100_bases
GACGGCGCAATGAGCTTTTTCAAACCTATGCTTGACCGTCTGTGGCGCATGGCCGACAGCGCCGGGGCCACCCGTGTGCT
TGCTATCGTTTCCTTTACCG

Product: protein-L-isoaspartate O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MIDKRRSRERMVREQLTARGITDPAVLAAMRKIPRHLFVQEALQAQAYEDHPLPIGYGQTISQPFIVALMSQILRVTPGM
RVLEIGTGSGYQAAVLAEMGAEVYTVERIAGLQAHARGLLRRLGYARIRTKLDDGTMGWPLAAPFDRIIVTAGGPGIPEP
LAEQLADPGTMAIPVGASRREQELYLMHKNDGALSYENYGKVAFVDLVGNHGW

Sequences:

>Translated_213_residues
MIDKRRSRERMVREQLTARGITDPAVLAAMRKIPRHLFVQEALQAQAYEDHPLPIGYGQTISQPFIVALMSQILRVTPGM
RVLEIGTGSGYQAAVLAEMGAEVYTVERIAGLQAHARGLLRRLGYARIRTKLDDGTMGWPLAAPFDRIIVTAGGPGIPEP
LAEQLADPGTMAIPVGASRREQELYLMHKNDGALSYENYGKVAFVDLVGNHGW
>Mature_213_residues
MIDKRRSRERMVREQLTARGITDPAVLAAMRKIPRHLFVQEALQAQAYEDHPLPIGYGQTISQPFIVALMSQILRVTPGM
RVLEIGTGSGYQAAVLAEMGAEVYTVERIAGLQAHARGLLRRLGYARIRTKLDDGTMGWPLAAPFDRIIVTAGGPGIPEP
LAEQLADPGTMAIPVGASRREQELYLMHKNDGALSYENYGKVAFVDLVGNHGW

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family

Homologues:

Organism=Homo sapiens, GI226530908, Length=175, Percent_Identity=30.2857142857143, Blast_Score=69, Evalue=3e-12,
Organism=Escherichia coli, GI1789100, Length=195, Percent_Identity=47.1794871794872, Blast_Score=164, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI71983477, Length=190, Percent_Identity=29.4736842105263, Blast_Score=67, Evalue=7e-12,
Organism=Drosophila melanogaster, GI17981723, Length=222, Percent_Identity=32.8828828828829, Blast_Score=76, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PIMT_DESDG (Q30ZM2)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_388569.1
- ProteinModelPortal:   Q30ZM2
- SMR:   Q30ZM2
- STRING:   Q30ZM2
- GeneID:   3757085
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_2077
- NMPDR:   fig|207559.3.peg.2225
- eggNOG:   COG2518
- HOGENOM:   HBG699907
- OMA:   MGAKEDL
- ProtClustDB:   CLSK705445
- BioCyc:   DDES207559:DDE_2077-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00090
- InterPro:   IPR000682
- PANTHER:   PTHR11579
- TIGRFAMs:   TIGR00080

Pfam domain/function: PF01135 PCMT

EC number: =2.1.1.77

Molecular weight: Translated: 23402; Mature: 23402

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: PS01279 PCMT

Important sites: ACT_SITE 62-62

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDKRRSRERMVREQLTARGITDPAVLAAMRKIPRHLFVQEALQAQAYEDHPLPIGYGQT
CCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCH
ISQPFIVALMSQILRVTPGMRVLEIGTGSGYQAAVLAEMGAEVYTVERIAGLQAHARGLL
HCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
RRLGYARIRTKLDDGTMGWPLAAPFDRIIVTAGGPGIPEPLAEQLADPGTMAIPVGASRR
HHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHCCCCCEEEECCCCCC
EQELYLMHKNDGALSYENYGKVAFVDLVGNHGW
CCEEEEEECCCCCEEECCCCCEEEEEECCCCCC
>Mature Secondary Structure
MIDKRRSRERMVREQLTARGITDPAVLAAMRKIPRHLFVQEALQAQAYEDHPLPIGYGQT
CCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCH
ISQPFIVALMSQILRVTPGMRVLEIGTGSGYQAAVLAEMGAEVYTVERIAGLQAHARGLL
HCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
RRLGYARIRTKLDDGTMGWPLAAPFDRIIVTAGGPGIPEPLAEQLADPGTMAIPVGASRR
HHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHCCCCCEEEECCCCCC
EQELYLMHKNDGALSYENYGKVAFVDLVGNHGW
CCEEEEEECCCCCEEECCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA