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The map label for this gene is cisA [H]

Identifier: 78356978

GI number: 78356978

Start: 1985707

End: 1987293

Strand: Direct

Name: cisA [H]

Synonym: Dde_1935

Alternate gene names: 78356978

Gene position: 1985707-1987293 (Clockwise)

Preceding gene: 78356977

Following gene: 78356979

Centisome position: 53.23

GC content: 60.74

Gene sequence:

>1587_bases
ATGCTTGATAACAGCAATGTCGCGCCGGGAAAGAACAAGACGCTGCGCTGTGCCATCTACACCCGCAAGAGCCACGAGGA
AGGGCTCGAACAGGAGTTCAACTCGTTGGATGCGCAACGGGAATCGGCGGAACACTATATCGAAGCTCAGAGGATGCGGG
GCTGGACGGCTCTGCCGGATCGCTATGACGATGGTGGTTTCTCGGGCGGGAACATGGAGCGTCCGGGGCTGCGCCGCCTG
CTGGCGGACATCGACGCCGGGAAGATCGATGTGATCGTCGTCTACAAGGTCGACCGGCTGTCCCGCTCGCTGCTGGACTT
CATGAAGATGATCGACCTCTTCAACGAGAAGGGTGTCAGCTTCGTCTCGGTCACCCAGCACTTCAGCACCACCGATCCCA
CCGGCCGAATGTTTCTCGGCATCCTGATCACCTTCGCCCAGTACGAGCGGGAGGTCATCGCCGAACGCATCCGGGACAAG
GTGGCGGCAGCCAAGCGCCGGGGGAAATACTGCGGCGGCGTACCCATCCTCGGATACGACGTCGACAGGGACAACAAGAA
GCTGCTGGTCAACCCAGATGAAGCCAGGACGGTGCAGTACATCTTCCGCCGCTTCATCCAGATCGGTTCGGCCAAGAAGC
TGGGCCAAGAGCTGAACGAACAGGGTTACCGCACCAAGGCCTGGACCACCAAGAAAGGCAAAGTGCGCGAGGGCTCCGAA
TGGAACACCGCCCATATCTACCGGCTGCTGAACAACCGGATCTATATCGGCGAGATCGCCCACAAGGATCGCAGCTACCC
TGGTGAGCACGAAGGGATCATCGACCGGGCGACCTGGGACAAGGTTCAGGCCATCCTGGAGGACAACAAACCGGTCAAGG
TATCGATGGCCAGAACCAAAATGGTCGCCCCGCTGAAAGGCGTCATCCGCTGCGGCCACTGCGGATGCGCGATGGGACCA
ACCTACGCCCGCAAGAACGGCCGCCACTACACCTATTACATCTGCCAAAAAGACAGCAAGCGGACCGTGAGCCGATGCCC
GCTCAAGCGGATTCCCGCCGGGGACATCGAGCAGGCCGTGATTGAGCAGTTGAGCGCCGTGTTCCGCACACCGACGCTGG
TGGCCAAGACCTACTTCGCGGCCCGGGACATCGAGCAGGCAGAGCGGGAGCGGTTGTTCAAGCAGAAAGCCCAACTCGAG
ATGGAGCTGTCGCAGGCGCGGGAGCAGGCTCTCGAATTAATGAAACCCGGCAGCGATCAGCCGGGCAAGACCGAGATGCT
GACGACGGTCAATCGCCAGGCGGTTGAGCTCTCGAAACAACTGACCCATGTGAGCGAGCGCTGCAGAGCCTACCGGGGGA
ACAGCATCACGGAACAGGATGTCTCGGAGGCCTTCCAGAATGTCGAGGGCTTCTGGGAAGACCTTTTCCCGGTGGAGCGA
AACCGGCTCATCCGCCTCCTGGTGGATAAGGTGGAGATCCGGGAGACCGGAATCGATATGGAGCTGCGCACCAACGGGCT
GACAACACTCATCGCCGAGCTGGCCGGTCTGGCATGCGAAGTCACTGAACGGAGGGCAAGCCGATGA

Upstream 100 bases:

>100_bases
CTCAGCGCGGTGGCCAGGGAGATCACCGGGACGCGCTGGAACGGCAAGGTCTTTTTCGGGCTGAAGAAGATTTACGGCAG
AAAAGCCGAGGGAGGTTCGG

Downstream 100 bases:

>100_bases
AAATAAAGCCGACCATTACCGTTGCCGACAACGGCAACCTGCAGATCCATATCCCGATGCTGATCCGGCGCATGCGCGGC
CGCAAGACGGTCATCGCTCC

Product: site-specific recombinase

Products: NA

Alternate protein names: Stage IV sporulation protein CA [H]

Number of amino acids: Translated: 528; Mature: 528

Protein sequence:

>528_residues
MLDNSNVAPGKNKTLRCAIYTRKSHEEGLEQEFNSLDAQRESAEHYIEAQRMRGWTALPDRYDDGGFSGGNMERPGLRRL
LADIDAGKIDVIVVYKVDRLSRSLLDFMKMIDLFNEKGVSFVSVTQHFSTTDPTGRMFLGILITFAQYEREVIAERIRDK
VAAAKRRGKYCGGVPILGYDVDRDNKKLLVNPDEARTVQYIFRRFIQIGSAKKLGQELNEQGYRTKAWTTKKGKVREGSE
WNTAHIYRLLNNRIYIGEIAHKDRSYPGEHEGIIDRATWDKVQAILEDNKPVKVSMARTKMVAPLKGVIRCGHCGCAMGP
TYARKNGRHYTYYICQKDSKRTVSRCPLKRIPAGDIEQAVIEQLSAVFRTPTLVAKTYFAARDIEQAERERLFKQKAQLE
MELSQAREQALELMKPGSDQPGKTEMLTTVNRQAVELSKQLTHVSERCRAYRGNSITEQDVSEAFQNVEGFWEDLFPVER
NRLIRLLVDKVEIRETGIDMELRTNGLTTLIAELAGLACEVTERRASR

Sequences:

>Translated_528_residues
MLDNSNVAPGKNKTLRCAIYTRKSHEEGLEQEFNSLDAQRESAEHYIEAQRMRGWTALPDRYDDGGFSGGNMERPGLRRL
LADIDAGKIDVIVVYKVDRLSRSLLDFMKMIDLFNEKGVSFVSVTQHFSTTDPTGRMFLGILITFAQYEREVIAERIRDK
VAAAKRRGKYCGGVPILGYDVDRDNKKLLVNPDEARTVQYIFRRFIQIGSAKKLGQELNEQGYRTKAWTTKKGKVREGSE
WNTAHIYRLLNNRIYIGEIAHKDRSYPGEHEGIIDRATWDKVQAILEDNKPVKVSMARTKMVAPLKGVIRCGHCGCAMGP
TYARKNGRHYTYYICQKDSKRTVSRCPLKRIPAGDIEQAVIEQLSAVFRTPTLVAKTYFAARDIEQAERERLFKQKAQLE
MELSQAREQALELMKPGSDQPGKTEMLTTVNRQAVELSKQLTHVSERCRAYRGNSITEQDVSEAFQNVEGFWEDLFPVER
NRLIRLLVDKVEIRETGIDMELRTNGLTTLIAELAGLACEVTERRASR
>Mature_528_residues
MLDNSNVAPGKNKTLRCAIYTRKSHEEGLEQEFNSLDAQRESAEHYIEAQRMRGWTALPDRYDDGGFSGGNMERPGLRRL
LADIDAGKIDVIVVYKVDRLSRSLLDFMKMIDLFNEKGVSFVSVTQHFSTTDPTGRMFLGILITFAQYEREVIAERIRDK
VAAAKRRGKYCGGVPILGYDVDRDNKKLLVNPDEARTVQYIFRRFIQIGSAKKLGQELNEQGYRTKAWTTKKGKVREGSE
WNTAHIYRLLNNRIYIGEIAHKDRSYPGEHEGIIDRATWDKVQAILEDNKPVKVSMARTKMVAPLKGVIRCGHCGCAMGP
TYARKNGRHYTYYICQKDSKRTVSRCPLKRIPAGDIEQAVIEQLSAVFRTPTLVAKTYFAARDIEQAERERLFKQKAQLE
MELSQAREQALELMKPGSDQPGKTEMLTTVNRQAVELSKQLTHVSERCRAYRGNSITEQDVSEAFQNVEGFWEDLFPVER
NRLIRLLVDKVEIRETGIDMELRTNGLTTLIAELAGLACEVTERRASR

Specific function: Putative site-specific recombinase having a very important role in sporulation. It probably plays a role in the recombination of spoIIIC and spoIVCB to form sigma K factor [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 recombinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=111, Percent_Identity=37.8378378378378, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1787827, Length=148, Percent_Identity=29.7297297297297, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1787638, Length=148, Percent_Identity=29.7297297297297, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011109
- InterPro:   IPR006118
- InterPro:   IPR006119 [H]

Pfam domain/function: PF07508 Recombinase; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 60257; Mature: 60257

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDNSNVAPGKNKTLRCAIYTRKSHEEGLEQEFNSLDAQRESAEHYIEAQRMRGWTALPD
CCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
RYDDGGFSGGNMERPGLRRLLADIDAGKIDVIVVYKVDRLSRSLLDFMKMIDLFNEKGVS
CCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHCCCE
FVSVTQHFSTTDPTGRMFLGILITFAQYEREVIAERIRDKVAAAKRRGKYCGGVPILGYD
EEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEE
VDRDNKKLLVNPDEARTVQYIFRRFIQIGSAKKLGQELNEQGYRTKAWTTKKGKVREGSE
CCCCCCEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEECCEECCCCCCCCCCC
WNTAHIYRLLNNRIYIGEIAHKDRSYPGEHEGIIDRATWDKVQAILEDNKPVKVSMARTK
CCHHHHHHHHHCCEEEEEHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEHHHHH
MVAPLKGVIRCGHCGCAMGPTYARKNGRHYTYYICQKDSKRTVSRCPLKRIPAGDIEQAV
HHHHHHHHHHCCCCCCCCCCHHHHCCCCEEEEEEEECCCCHHHHHCCCCCCCCCCHHHHH
IEQLSAVFRTPTLVAKTYFAARDIEQAERERLFKQKAQLEMELSQAREQALELMKPGSDQ
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PGKTEMLTTVNRQAVELSKQLTHVSERCRAYRGNSITEQDVSEAFQNVEGFWEDLFPVER
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHH
NRLIRLLVDKVEIRETGIDMELRTNGLTTLIAELAGLACEVTERRASR
HHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLDNSNVAPGKNKTLRCAIYTRKSHEEGLEQEFNSLDAQRESAEHYIEAQRMRGWTALPD
CCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
RYDDGGFSGGNMERPGLRRLLADIDAGKIDVIVVYKVDRLSRSLLDFMKMIDLFNEKGVS
CCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHCCCE
FVSVTQHFSTTDPTGRMFLGILITFAQYEREVIAERIRDKVAAAKRRGKYCGGVPILGYD
EEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEE
VDRDNKKLLVNPDEARTVQYIFRRFIQIGSAKKLGQELNEQGYRTKAWTTKKGKVREGSE
CCCCCCEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEECCEECCCCCCCCCCC
WNTAHIYRLLNNRIYIGEIAHKDRSYPGEHEGIIDRATWDKVQAILEDNKPVKVSMARTK
CCHHHHHHHHHCCEEEEEHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEHHHHH
MVAPLKGVIRCGHCGCAMGPTYARKNGRHYTYYICQKDSKRTVSRCPLKRIPAGDIEQAV
HHHHHHHHHHCCCCCCCCCCHHHHCCCCEEEEEEEECCCCHHHHHCCCCCCCCCCHHHHH
IEQLSAVFRTPTLVAKTYFAARDIEQAERERLFKQKAQLEMELSQAREQALELMKPGSDQ
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PGKTEMLTTVNRQAVELSKQLTHVSERCRAYRGNSITEQDVSEAFQNVEGFWEDLFPVER
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHH
NRLIRLLVDKVEIRETGIDMELRTNGLTTLIAELAGLACEVTERRASR
HHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2105293; 7704261; 8969508; 9384377 [H]