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The map label for this gene is gltB [C]

Identifier: 78356857

GI number: 78356857

Start: 1864178

End: 1865701

Strand: Direct

Name: gltB [C]

Synonym: Dde_1814

Alternate gene names: 78356857

Gene position: 1864178-1865701 (Clockwise)

Preceding gene: 78356856

Following gene: 78356858

Centisome position: 49.97

GC content: 56.96

Gene sequence:

>1524_bases
TTGCCTTTCAAGCCGATAAGTCAAAAATTCACAGACTTTGTCATTGTCCGCGATGATTCGCGCTGTATCAACTGCGAGGT
CTGTGTGCGACAGTGCTCCTATGAGGCACATTACTGGGACGACACCAGACAGCGTGTCAGCTTCGACAGTTCAAAATGCA
CCGGCTGCCACAGATGCGCGGCGCAGTGCCCCACAAGCTGCCTGACCATACGCAGAAATCCTTCGGAATTCCGTGAAAAC
GCTTTCTGGACACCTGCGTTCATCAAACACATATACAAGCAGAGCGATACAGGCGGCATCCTGCTTTCTGGATCAGGCAG
TCCGGCGGACAAGCCCGTCTACTGGGACAACCTGCAGCTTGACGCAAGCCAGGTCACCAATCCGTCGATTGATCCGCTGC
GTGAACCGATGGAGCTGCGCACCTATCTGGGAGCCAAACCGGATATCGCGGCTTTTGAAGAAACAGCCGGCACATTCAGG
CTGAAGGGAGAAATGCCTCCGCAGATCCGGCTGGAGTATCCGCTGATGTTTGCAGCCATGTCGTTCGGATCCATCAATTT
CAACCTGCACAAAGCCATGGCTCAGGCCGCCACGGAACTGGGCATAGTCTACAATACGGGAGAGGGCGGGCTCCATCCTT
CACTGTACGGCTACGGGGCCAATACCATTGTACAGGTAGCCTCCGGCCGTTTCGGGGTGCATAAAGACTACCTGAACGCA
GGTGCGGCAGTGGAAATAAAGGTTGGTCAGGGGGCAAAGCCGGGTATCGGCGGACACCTGCCGGGTGAAAAAATTGACGA
AGAAGTGTCACGCACCCGCATGGTACCTCTCGGTTCGGACGCCATATCTCCGGCTCCGCACCACGACATCTACTCCATTG
AAGATCTGCTGCAGCTTATCTATGCCATCAAGGAAGCCACCCGATACCGTGTTCCCGTTTCCGTCAAGATTGCAGCCGTT
CACAATGCTCCGGCCATTGCCTCGGGCATAGTCCGGGCCGGTGCCGACATTGTGGTGATTGACGGATTCAGAGGTGGCAC
AGGTGCTGCGCCAACCATGATCCGCGACAACGTGGGCATACCCATCGAACTGGCCCTTGCCGCGGTAGACAACCGCCTGC
GCGATGAGGGTATCCGCAACCATGCCTCGCTTGTGGTTTCGGGAGGAATGCGCTGCAGCGCGGACGTTGTGAAGGCCATA
GCGCTGGGTGCGGACGCTGTTTACATAGGAACTGCGGCTCTTGTGGCTGTAGGATGCACCCTGTGCGGCCGCTGTTATAC
CGGCAAATGTCCGTGGGGTATCGCCACCAATGAAGCACGCCTGAAAAAACGCCAGAATCCCGATGTGGCAGCCAGACGCC
TTGCCAATCTTATCCGCGCATGGGGGCACGAAATACAGGAAATGCTGGGCGGCATGGGCCTGAACTCCATTGAAAGCCTG
CGGGGCAACCGCGACAAGCTGCGCGGCATCGGCCTTAATCAGGTGGAACTGGACATACTGGGCGTTAAACACGCGGGCAG
ATAG

Upstream 100 bases:

>100_bases
ATTCCTAACATCAAGAGCGCATCTTCCTGCTGCTTATTTGTGCAATGCGCAAAATAAACATTTTTGTCACAGATGCGCCA
TCCAGCAAAGGAGGCACGCT

Downstream 100 bases:

>100_bases
CCCGAAAATGCTCGGGCGGCTCTTTTTGCCGTCAGGCCGGACTAATCCGGCGACAGGAGAACGACATGAAAGCTCCGGAT
ACATTCTGGCAGTTTGATAA

Product: glutamate synthase (NADPH) GltB2 subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 507; Mature: 506

Protein sequence:

>507_residues
MPFKPISQKFTDFVIVRDDSRCINCEVCVRQCSYEAHYWDDTRQRVSFDSSKCTGCHRCAAQCPTSCLTIRRNPSEFREN
AFWTPAFIKHIYKQSDTGGILLSGSGSPADKPVYWDNLQLDASQVTNPSIDPLREPMELRTYLGAKPDIAAFEETAGTFR
LKGEMPPQIRLEYPLMFAAMSFGSINFNLHKAMAQAATELGIVYNTGEGGLHPSLYGYGANTIVQVASGRFGVHKDYLNA
GAAVEIKVGQGAKPGIGGHLPGEKIDEEVSRTRMVPLGSDAISPAPHHDIYSIEDLLQLIYAIKEATRYRVPVSVKIAAV
HNAPAIASGIVRAGADIVVIDGFRGGTGAAPTMIRDNVGIPIELALAAVDNRLRDEGIRNHASLVVSGGMRCSADVVKAI
ALGADAVYIGTAALVAVGCTLCGRCYTGKCPWGIATNEARLKKRQNPDVAARRLANLIRAWGHEIQEMLGGMGLNSIESL
RGNRDKLRGIGLNQVELDILGVKHAGR

Sequences:

>Translated_507_residues
MPFKPISQKFTDFVIVRDDSRCINCEVCVRQCSYEAHYWDDTRQRVSFDSSKCTGCHRCAAQCPTSCLTIRRNPSEFREN
AFWTPAFIKHIYKQSDTGGILLSGSGSPADKPVYWDNLQLDASQVTNPSIDPLREPMELRTYLGAKPDIAAFEETAGTFR
LKGEMPPQIRLEYPLMFAAMSFGSINFNLHKAMAQAATELGIVYNTGEGGLHPSLYGYGANTIVQVASGRFGVHKDYLNA
GAAVEIKVGQGAKPGIGGHLPGEKIDEEVSRTRMVPLGSDAISPAPHHDIYSIEDLLQLIYAIKEATRYRVPVSVKIAAV
HNAPAIASGIVRAGADIVVIDGFRGGTGAAPTMIRDNVGIPIELALAAVDNRLRDEGIRNHASLVVSGGMRCSADVVKAI
ALGADAVYIGTAALVAVGCTLCGRCYTGKCPWGIATNEARLKKRQNPDVAARRLANLIRAWGHEIQEMLGGMGLNSIESL
RGNRDKLRGIGLNQVELDILGVKHAGR
>Mature_506_residues
PFKPISQKFTDFVIVRDDSRCINCEVCVRQCSYEAHYWDDTRQRVSFDSSKCTGCHRCAAQCPTSCLTIRRNPSEFRENA
FWTPAFIKHIYKQSDTGGILLSGSGSPADKPVYWDNLQLDASQVTNPSIDPLREPMELRTYLGAKPDIAAFEETAGTFRL
KGEMPPQIRLEYPLMFAAMSFGSINFNLHKAMAQAATELGIVYNTGEGGLHPSLYGYGANTIVQVASGRFGVHKDYLNAG
AAVEIKVGQGAKPGIGGHLPGEKIDEEVSRTRMVPLGSDAISPAPHHDIYSIEDLLQLIYAIKEATRYRVPVSVKIAAVH
NAPAIASGIVRAGADIVVIDGFRGGTGAAPTMIRDNVGIPIELALAAVDNRLRDEGIRNHASLVVSGGMRCSADVVKAIA
LGADAVYIGTAALVAVGCTLCGRCYTGKCPWGIATNEARLKKRQNPDVAARRLANLIRAWGHEIQEMLGGMGLNSIESLR
GNRDKLRGIGLNQVELDILGVKHAGR

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0069

COG function: function code E; Glutamate synthase domain 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI308199519, Length=312, Percent_Identity=38.7820512820513, Blast_Score=200, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI17570289, Length=331, Percent_Identity=37.7643504531722, Blast_Score=205, Evalue=4e-53,
Organism=Saccharomyces cerevisiae, GI6320030, Length=331, Percent_Identity=34.7432024169184, Blast_Score=195, Evalue=1e-50,
Organism=Drosophila melanogaster, GI28574881, Length=331, Percent_Identity=36.8580060422961, Blast_Score=196, Evalue=4e-50,
Organism=Drosophila melanogaster, GI24665539, Length=331, Percent_Identity=36.8580060422961, Blast_Score=196, Evalue=4e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR013785
- InterPro:   IPR002932 [H]

Pfam domain/function: PF01645 Glu_synthase [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 54832; Mature: 54701

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFKPISQKFTDFVIVRDDSRCINCEVCVRQCSYEAHYWDDTRQRVSFDSSKCTGCHRCA
CCCCCCCCCCCCEEEEECCCCEEEHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCHHHHHH
AQCPTSCLTIRRNPSEFRENAFWTPAFIKHIYKQSDTGGILLSGSGSPADKPVYWDNLQL
HHCCCCEEEEECCHHHHHHCCCCCHHHHHHHHHCCCCCCEEEECCCCCCCCCEEECCCEE
DASQVTNPSIDPLREPMELRTYLGAKPDIAAFEETAGTFRLKGEMPPQIRLEYPLMFAAM
CHHHCCCCCHHHHHCHHHHHHHCCCCCCHHHHHHCCCEEEECCCCCCCEEEECCCEEEHH
SFGSINFNLHKAMAQAATELGIVYNTGEGGLHPSLYGYGANTIVQVASGRFGVHKDYLNA
HCCCCCHHHHHHHHHHHHHCCEEEECCCCCCCCCEECCCCCCEEEECCCCCCCCHHHHCC
GAAVEIKVGQGAKPGIGGHLPGEKIDEEVSRTRMVPLGSDAISPAPHHDIYSIEDLLQLI
CCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHH
YAIKEATRYRVPVSVKIAAVHNAPAIASGIVRAGADIVVIDGFRGGTGAAPTMIRDNVGI
HHHHHHHHEECCEEEEEEEECCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCEEECCCCC
PIELALAAVDNRLRDEGIRNHASLVVSGGMRCSADVVKAIALGADAVYIGTAALVAVGCT
CHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHCCCEEHHHHHHHHHHHHH
LCGRCYTGKCPWGIATNEARLKKRQNPDVAARRLANLIRAWGHEIQEMLGGMGLNSIESL
HHCCCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
RGNRDKLRGIGLNQVELDILGVKHAGR
CCCHHHHCCCCCCEEEEEEEECCCCCC
>Mature Secondary Structure 
PFKPISQKFTDFVIVRDDSRCINCEVCVRQCSYEAHYWDDTRQRVSFDSSKCTGCHRCA
CCCCCCCCCCCEEEEECCCCEEEHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCHHHHHH
AQCPTSCLTIRRNPSEFRENAFWTPAFIKHIYKQSDTGGILLSGSGSPADKPVYWDNLQL
HHCCCCEEEEECCHHHHHHCCCCCHHHHHHHHHCCCCCCEEEECCCCCCCCCEEECCCEE
DASQVTNPSIDPLREPMELRTYLGAKPDIAAFEETAGTFRLKGEMPPQIRLEYPLMFAAM
CHHHCCCCCHHHHHCHHHHHHHCCCCCCHHHHHHCCCEEEECCCCCCCEEEECCCEEEHH
SFGSINFNLHKAMAQAATELGIVYNTGEGGLHPSLYGYGANTIVQVASGRFGVHKDYLNA
HCCCCCHHHHHHHHHHHHHCCEEEECCCCCCCCCEECCCCCCEEEECCCCCCCCHHHHCC
GAAVEIKVGQGAKPGIGGHLPGEKIDEEVSRTRMVPLGSDAISPAPHHDIYSIEDLLQLI
CCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHH
YAIKEATRYRVPVSVKIAAVHNAPAIASGIVRAGADIVVIDGFRGGTGAAPTMIRDNVGI
HHHHHHHHEECCEEEEEEEECCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCEEECCCCC
PIELALAAVDNRLRDEGIRNHASLVVSGGMRCSADVVKAIALGADAVYIGTAALVAVGCT
CHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHCCCEEHHHHHHHHHHHHH
LCGRCYTGKCPWGIATNEARLKKRQNPDVAARRLANLIRAWGHEIQEMLGGMGLNSIESL
HHCCCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
RGNRDKLRGIGLNQVELDILGVKHAGR
CCCHHHHCCCCCCEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]