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The map label for this gene is prfB [H]

Identifier: 78356848

GI number: 78356848

Start: 1853187

End: 1854263

Strand: Reverse

Name: prfB [H]

Synonym: Dde_1805

Alternate gene names: 78356848

Gene position: 1854263-1853187 (Counterclockwise)

Preceding gene: 78356849

Following gene: 78356847

Centisome position: 49.71

GC content: 54.22

Gene sequence:

>1077_bases
ATGCCCTTCTCCAGCAGTTTGAAACGCTCTGGGGGCGCCTTTGACCACGCGCAGAGTAAATACAGGCTGGAAGAGATAGA
AAAACAGCTGTCCAGTCCAGACGCGTGGAATGCGCCGGAAAAACTTACGCCGGTGCTGCGGGAAAAAAGTCAGCTTGAAA
ACAGCGTTGCGCGGCTGGACGCACTGCGGCAGGCCAAGGACGACATGCAGGGCTGGCTTGAGCTGGCTGAAGAAGAAAAT
ACGCAGGAAGTTCTTGAACAACTGGAAGAACAGGCCGGAATTCTGGAAGCTCTGCTGGAAGAGACCGAAGTCGGCCTGCT
GCTTTCAGCAGAAGAAGACAAGCACGCTGCCATTCTTGAAATTCATCCCGGTGCCGGAGGCACGGAAGCACAGGACTGGG
CGGAAATGTTGCTGCGCATGTATGTGCGCTGGGCGGAAAGCCATGAATTTAAAGCCGAATACCTTGATTATCTTGACGGA
GATGAAGCCGGCATCAAAAGCGTGACGCTGAAGATTGACGGCACCAATGCCTACGGGCTGCTTAAAGGTGAAAAAGGTAT
CCACAGACTTATCCGTATATCGCCTTATGATGCCTCCGGCAGACGTCATACATCCTTTGCATCGGTAGATGTAATTCCAG
ATGCAGGCGAAGAGATCACCATCGATATCAAGGATTCTGACCTGCGCATTGATATTTTCCGCTCCAGCGGACCGGGAGGA
CAGAGCGTAAACACCACAAGCTCCGCCGTGCGTGTGACACATATCCCCACCGGTATCACTGCCCAGTGCCAGAACGAAAA
ATCGCAGCATCACAATAAAGAGACTGCCATGCAGATATTGCGGGCGCGGTTGTATGAGGCTGAAATACGCCGGCGTGACG
CTGAAAAGCAGGCGGACTACGCCAGCAAGGAAGCCATCGGCTGGGGCAGCCAGATACGCACCTATACGCTGCAGCCGTAC
AGGCTGGTGAAGGATCACCGTTCCAACTTTGAAGTGGGCGATGTGGAAGCGGTGCTTGACGGCGAGCTTGATGCGCTGAT
GCGCAACCACCTGCTCTACCAGCATGAGCGACGATAG

Upstream 100 bases:

>100_bases
CTGTACGACGGCATTCTGTCTGCTGCAGACAGGACGGCGGCAAACGCCACAATAACAGGATATTTTATGCTTCAACTTTC
CGAACTCCGTTCCAGAGGCA

Downstream 100 bases:

>100_bases
TATGCAGAATGAAGAGCATGATGCCAGCTCGCGGAGAGCTGAACAGCATAAGCATGAAGAGCTTGTCGAAGCCGCCACCG
AGGAAATTCAGACCCTGCGG

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 358; Mature: 357

Protein sequence:

>358_residues
MPFSSSLKRSGGAFDHAQSKYRLEEIEKQLSSPDAWNAPEKLTPVLREKSQLENSVARLDALRQAKDDMQGWLELAEEEN
TQEVLEQLEEQAGILEALLEETEVGLLLSAEEDKHAAILEIHPGAGGTEAQDWAEMLLRMYVRWAESHEFKAEYLDYLDG
DEAGIKSVTLKIDGTNAYGLLKGEKGIHRLIRISPYDASGRRHTSFASVDVIPDAGEEITIDIKDSDLRIDIFRSSGPGG
QSVNTTSSAVRVTHIPTGITAQCQNEKSQHHNKETAMQILRARLYEAEIRRRDAEKQADYASKEAIGWGSQIRTYTLQPY
RLVKDHRSNFEVGDVEAVLDGELDALMRNHLLYQHERR

Sequences:

>Translated_358_residues
MPFSSSLKRSGGAFDHAQSKYRLEEIEKQLSSPDAWNAPEKLTPVLREKSQLENSVARLDALRQAKDDMQGWLELAEEEN
TQEVLEQLEEQAGILEALLEETEVGLLLSAEEDKHAAILEIHPGAGGTEAQDWAEMLLRMYVRWAESHEFKAEYLDYLDG
DEAGIKSVTLKIDGTNAYGLLKGEKGIHRLIRISPYDASGRRHTSFASVDVIPDAGEEITIDIKDSDLRIDIFRSSGPGG
QSVNTTSSAVRVTHIPTGITAQCQNEKSQHHNKETAMQILRARLYEAEIRRRDAEKQADYASKEAIGWGSQIRTYTLQPY
RLVKDHRSNFEVGDVEAVLDGELDALMRNHLLYQHERR
>Mature_357_residues
PFSSSLKRSGGAFDHAQSKYRLEEIEKQLSSPDAWNAPEKLTPVLREKSQLENSVARLDALRQAKDDMQGWLELAEEENT
QEVLEQLEEQAGILEALLEETEVGLLLSAEEDKHAAILEIHPGAGGTEAQDWAEMLLRMYVRWAESHEFKAEYLDYLDGD
EAGIKSVTLKIDGTNAYGLLKGEKGIHRLIRISPYDASGRRHTSFASVDVIPDAGEEITIDIKDSDLRIDIFRSSGPGGQ
SVNTTSSAVRVTHIPTGITAQCQNEKSQHHNKETAMQILRARLYEAEIRRRDAEKQADYASKEAIGWGSQIRTYTLQPYR
LVKDHRSNFEVGDVEAVLDGELDALMRNHLLYQHERR

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=291, Percent_Identity=38.1443298969072, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI34577120, Length=329, Percent_Identity=35.258358662614, Blast_Score=159, Evalue=3e-39,
Organism=Homo sapiens, GI166795305, Length=186, Percent_Identity=38.1720430107527, Blast_Score=115, Evalue=7e-26,
Organism=Escherichia coli, GI2367172, Length=340, Percent_Identity=49.7058823529412, Blast_Score=337, Evalue=5e-94,
Organism=Escherichia coli, GI1787462, Length=240, Percent_Identity=42.5, Blast_Score=189, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI17542784, Length=338, Percent_Identity=31.9526627218935, Blast_Score=124, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6321295, Length=235, Percent_Identity=40, Blast_Score=157, Evalue=3e-39,
Organism=Drosophila melanogaster, GI19921226, Length=290, Percent_Identity=36.2068965517241, Blast_Score=152, Evalue=4e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40324; Mature: 40193

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFSSSLKRSGGAFDHAQSKYRLEEIEKQLSSPDAWNAPEKLTPVLREKSQLENSVARLD
CCCCHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ALRQAKDDMQGWLELAEEENTQEVLEQLEEQAGILEALLEETEVGLLLSAEEDKHAAILE
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEEE
IHPGAGGTEAQDWAEMLLRMYVRWAESHEFKAEYLDYLDGDEAGIKSVTLKIDGTNAYGL
EECCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCEEEEEEEECCCCEEE
LKGEKGIHRLIRISPYDASGRRHTSFASVDVIPDAGEEITIDIKDSDLRIDIFRSSGPGG
EECCHHHHHHEEECCCCCCCCCCCCEEEEEECCCCCCEEEEEEECCCEEEEEEECCCCCC
QSVNTTSSAVRVTHIPTGITAQCQNEKSQHHNKETAMQILRARLYEAEIRRRDAEKQADY
CCCCCCCCEEEEEECCCCCCHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASKEAIGWGSQIRTYTLQPYRLVKDHRSNFEVGDVEAVLDGELDALMRNHLLYQHERR
HHHHHCCCCCCCEEEECCHHHHHHHHCCCCCCCCCHHHHCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PFSSSLKRSGGAFDHAQSKYRLEEIEKQLSSPDAWNAPEKLTPVLREKSQLENSVARLD
CCCHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ALRQAKDDMQGWLELAEEENTQEVLEQLEEQAGILEALLEETEVGLLLSAEEDKHAAILE
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEEE
IHPGAGGTEAQDWAEMLLRMYVRWAESHEFKAEYLDYLDGDEAGIKSVTLKIDGTNAYGL
EECCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCEEEEEEEECCCCEEE
LKGEKGIHRLIRISPYDASGRRHTSFASVDVIPDAGEEITIDIKDSDLRIDIFRSSGPGG
EECCHHHHHHEEECCCCCCCCCCCCEEEEEECCCCCCEEEEEEECCCEEEEEEECCCCCC
QSVNTTSSAVRVTHIPTGITAQCQNEKSQHHNKETAMQILRARLYEAEIRRRDAEKQADY
CCCCCCCCEEEEEECCCCCCHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASKEAIGWGSQIRTYTLQPYRLVKDHRSNFEVGDVEAVLDGELDALMRNHLLYQHERR
HHHHHCCCCCCCEEEECCHHHHHHHHCCCCCCCCCHHHHCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA