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The map label for this gene is ispDF

Identifier: 78356769

GI number: 78356769

Start: 1783902

End: 1785101

Strand: Reverse

Name: ispDF

Synonym: Dde_1726

Alternate gene names: 78356769

Gene position: 1785101-1783902 (Counterclockwise)

Preceding gene: 78356770

Following gene: 78356768

Centisome position: 47.85

GC content: 56.25

Gene sequence:

>1200_bases
ATGCACACATGGGCACTTCTTCTGGCGGCAGGCCAGAGCTCCAGAATAGCGGCAGCATGTCCGGGCGTCCGCAAACAGTT
TCTGCTTTGGCAGAACGCCCCTCTTTTCTGGCATTCCGCAGTCATGCTTTCCCGCGTCAGTCGCATGCGGGGCATAATTT
TTGTTTTTCCTGAAGACATGCTGGAAGAGGCAACCGCCATGGTCAGGGAACTGGATGCAGGCCGGGCACTGGGCATACCA
TGGAAAGCCGTATCAGGCGGTGCCCGTCGGCAGGATTCCGTCGCCAGCGGTCTCGGGCAGCTTCCGGCAGAGTGCGATAC
GGTGCTTGTGCATGATGCAGCAAGACCTTTTGCCAGCGCGTCACTTACCAACAGCATTCTGGATGCGTTGCAGGCCGGAG
CTGAAGGAGTGGTGCCGGCACTGGCCGTTACCGACACTATAAAGGTGGTAGAAGACGGCGCGGTGCTTTCAACTCCGGAC
AGGACAAAGCTCGTGGCCGTTCAGACTCCACAGGGTTTCAGCCTTCAGGCGCTGCTCGGTGCGCACCGTCATTGCGCAGA
AAAAGCACTGGCCGTCACCGATGATGCATCCATGCTCGAACTGCTCGGACAACATACTGTCATAACAGTGGAAGGTGAGG
CATCCAACATAAAAGTGACGCATCCGGAGGATCTGACCATGCTGCATTCTTCAGAAAAAAAGAATATGCAAGTCCCCTGT
GTCGGATGGGGATACGACGTGCACCGGTTCGGTTCTGACGGCCGTCCCATGAAACTTGGCGGTGTGCCCATAGCAGGCGG
CCCGGGCGTTATAGCGCATTCAGACGGTGACGTGCTGCTGCATGCCCTTACAGATGCCGTGCTCGGCTGCATGGGGCTGG
GAGACATCGGCATGCTGTTTCCCGACACAGATGCGGCGTACGATAATGCTGATAGTGCCGTCATGCTGAACGAGGTGCTT
GAAAAAGCCAGAAATGCAGGCCTGCTGATTACGCACGTTGATCTGACGATAATAACGCAGATTCCCAGAATCACACCATG
GCGCGACCAGATACATAAAAATATCTGCAGAATCACCCGCCTGGACGCATCTTCTGTCAACCTGAAGGCGACAACAGAAG
AAAAACTGGGCTTCACCGGGGAAAAGAAAGGGATAAAGGCAGTTGCGGCCGTGACCGCTCTGCGCCGGGTCTCTTCCTGA

Upstream 100 bases:

>100_bases
TTATGGAAGAACAGAACCCGGCTTATAGTCCGGCTCCCGTCTGTACCAACACACAGGGGCGCTCATTGCGCCCCTGTTTT
TTTCACAGAGAGACATAACC

Downstream 100 bases:

>100_bases
CCGGCTTTTAGTTTTCCGCCTCTTGCACCCTGCGGTTCAACAATATAACATACAAAGACAACTCAGAAAATACGGAAATG
CCCAACCAACCTGAACAGCA

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase; 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT; 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase; MECDP-synthase; MECPS

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MHTWALLLAAGQSSRIAAACPGVRKQFLLWQNAPLFWHSAVMLSRVSRMRGIIFVFPEDMLEEATAMVRELDAGRALGIP
WKAVSGGARRQDSVASGLGQLPAECDTVLVHDAARPFASASLTNSILDALQAGAEGVVPALAVTDTIKVVEDGAVLSTPD
RTKLVAVQTPQGFSLQALLGAHRHCAEKALAVTDDASMLELLGQHTVITVEGEASNIKVTHPEDLTMLHSSEKKNMQVPC
VGWGYDVHRFGSDGRPMKLGGVPIAGGPGVIAHSDGDVLLHALTDAVLGCMGLGDIGMLFPDTDAAYDNADSAVMLNEVL
EKARNAGLLITHVDLTIITQIPRITPWRDQIHKNICRITRLDASSVNLKATTEEKLGFTGEKKGIKAVAAVTALRRVSS

Sequences:

>Translated_399_residues
MHTWALLLAAGQSSRIAAACPGVRKQFLLWQNAPLFWHSAVMLSRVSRMRGIIFVFPEDMLEEATAMVRELDAGRALGIP
WKAVSGGARRQDSVASGLGQLPAECDTVLVHDAARPFASASLTNSILDALQAGAEGVVPALAVTDTIKVVEDGAVLSTPD
RTKLVAVQTPQGFSLQALLGAHRHCAEKALAVTDDASMLELLGQHTVITVEGEASNIKVTHPEDLTMLHSSEKKNMQVPC
VGWGYDVHRFGSDGRPMKLGGVPIAGGPGVIAHSDGDVLLHALTDAVLGCMGLGDIGMLFPDTDAAYDNADSAVMLNEVL
EKARNAGLLITHVDLTIITQIPRITPWRDQIHKNICRITRLDASSVNLKATTEEKLGFTGEKKGIKAVAAVTALRRVSS
>Mature_399_residues
MHTWALLLAAGQSSRIAAACPGVRKQFLLWQNAPLFWHSAVMLSRVSRMRGIIFVFPEDMLEEATAMVRELDAGRALGIP
WKAVSGGARRQDSVASGLGQLPAECDTVLVHDAARPFASASLTNSILDALQAGAEGVVPALAVTDTIKVVEDGAVLSTPD
RTKLVAVQTPQGFSLQALLGAHRHCAEKALAVTDDASMLELLGQHTVITVEGEASNIKVTHPEDLTMLHSSEKKNMQVPC
VGWGYDVHRFGSDGRPMKLGGVPIAGGPGVIAHSDGDVLLHALTDAVLGCMGLGDIGMLFPDTDAAYDNADSAVMLNEVL
EKARNAGLLITHVDLTIITQIPRITPWRDQIHKNICRITRLDASSVNLKATTEEKLGFTGEKKGIKAVAAVTALRRVSS

Specific function: Bifunctional enzyme that catalyzes the formation of 4- diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl- D-erythritol 4-phosphate (MEP) (ispD), and converts 4- diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C- methyl-D-erythri

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=159, Percent_Identity=47.1698113207547, Blast_Score=118, Evalue=5e-28,
Organism=Escherichia coli, GI1789104, Length=226, Percent_Identity=34.5132743362832, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPDF_DESDG (Q310X3)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_388218.1
- HSSP:   P62617
- ProteinModelPortal:   Q310X3
- SMR:   Q310X3
- STRING:   Q310X3
- GeneID:   3756724
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_1726
- NMPDR:   fig|207559.3.peg.1539
- eggNOG:   COG1211
- HOGENOM:   HBG672839
- OMA:   IVLIHDA
- ProtClustDB:   CLSK705254
- BioCyc:   DDES207559:DDE_1726-MONOMER
- HAMAP:   MF_01520
- InterPro:   IPR001228
- InterPro:   IPR018294
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00453
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF01128 IspD; PF02542 YgbB; SSF69765 YgbB_synth

EC number: =2.7.7.60; =4.6.1.12

Molecular weight: Translated: 42461; Mature: 42461

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS01295 ISPD; PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHTWALLLAAGQSSRIAAACPGVRKQFLLWQNAPLFWHSAVMLSRVSRMRGIIFVFPEDM
CCCEEEEEECCCCCCEEECCCCCHHHHEEECCCCHHHHHHHHHHHHHHHCCEEEECCHHH
LEEATAMVRELDAGRALGIPWKAVSGGARRQDSVASGLGQLPAECDTVLVHDAARPFASA
HHHHHHHHHHHCCCCCCCCCHHHCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCHHHH
SLTNSILDALQAGAEGVVPALAVTDTIKVVEDGAVLSTPDRTKLVAVQTPQGFSLQALLG
HHHHHHHHHHHCCCCCCCCHHHHHHHHEEEECCCEEECCCCCEEEEEECCCCCCHHHHHH
AHRHCAEKALAVTDDASMLELLGQHTVITVEGEASNIKVTHPEDLTMLHSSEKKNMQVPC
HHHHHHHHHEEECCCHHHHHHHCCCEEEEEECCCCEEEEECCCCHHEEECCCCCCCCCEE
VGWGYDVHRFGSDGRPMKLGGVPIAGGPGVIAHSDGDVLLHALTDAVLGCMGLGDIGMLF
EECCCCHHHCCCCCCEEEECCEEECCCCCEEEECCCCHHHHHHHHHHHHHCCCCCCCEEC
PDTDAAYDNADSAVMLNEVLEKARNAGLLITHVDLTIITQIPRITPWRDQIHKNICRITR
CCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECEEEEECCCCCCCHHHHHHHHHHHHEE
LDASSVNLKATTEEKLGFTGEKKGIKAVAAVTALRRVSS
CCCCCCEEEECCHHHCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MHTWALLLAAGQSSRIAAACPGVRKQFLLWQNAPLFWHSAVMLSRVSRMRGIIFVFPEDM
CCCEEEEEECCCCCCEEECCCCCHHHHEEECCCCHHHHHHHHHHHHHHHCCEEEECCHHH
LEEATAMVRELDAGRALGIPWKAVSGGARRQDSVASGLGQLPAECDTVLVHDAARPFASA
HHHHHHHHHHHCCCCCCCCCHHHCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCHHHH
SLTNSILDALQAGAEGVVPALAVTDTIKVVEDGAVLSTPDRTKLVAVQTPQGFSLQALLG
HHHHHHHHHHHCCCCCCCCHHHHHHHHEEEECCCEEECCCCCEEEEEECCCCCCHHHHHH
AHRHCAEKALAVTDDASMLELLGQHTVITVEGEASNIKVTHPEDLTMLHSSEKKNMQVPC
HHHHHHHHHEEECCCHHHHHHHCCCEEEEEECCCCEEEEECCCCHHEEECCCCCCCCCEE
VGWGYDVHRFGSDGRPMKLGGVPIAGGPGVIAHSDGDVLLHALTDAVLGCMGLGDIGMLF
EECCCCHHHCCCCCCEEEECCEEECCCCCEEEECCCCHHHHHHHHHHHHHCCCCCCCEEC
PDTDAAYDNADSAVMLNEVLEKARNAGLLITHVDLTIITQIPRITPWRDQIHKNICRITR
CCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECEEEEECCCCCCCHHHHHHHHHHHHEE
LDASSVNLKATTEEKLGFTGEKKGIKAVAAVTALRRVSS
CCCCCCEEEECCHHHCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA