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The map label for this gene is oprF [H]

Identifier: 78356732

GI number: 78356732

Start: 1740755

End: 1741813

Strand: Reverse

Name: oprF [H]

Synonym: Dde_1689

Alternate gene names: 78356732

Gene position: 1741813-1740755 (Counterclockwise)

Preceding gene: 78356733

Following gene: 78356731

Centisome position: 46.69

GC content: 53.92

Gene sequence:

>1059_bases
ATGAAACTAACACTCAAACCTGTCTTCATGTTCCTGCTGGCCATGCTGATCATGGCTCCGGCATTTTCCCATGCAGCGGA
CATGGAGTACATCCCCAAAATTGCCAGCTTCGACTTTTTTGTTGATGATTCCGGCTCTATGATGATGACGCATCAGAAGC
TGGAAAAACCGAAAATCGAAGTGGCCAAAGACGTGCTCAGCAAGATCAACAATGCAATACCTGCGCTGAAATACAACGCA
AGCATGCATATGTTTGCTCCTTACGAAGAAGTGGTGCCTTACGGTCCCTACTCCAAAAGCGGCTTTGAGCAGGGCATCAT
GAGCCTGAAGGCCGACAAGGAGATCTTTGCCCGCCGTACCCCCATGGGGGACGGTCTTAACATGCTGCGCCCCGCAGCGG
ACAATATGGCCCGCAATGCCGCTGTCATCATAGTCAGCGACGGCGAATCCAACTTCGGCAGCGACCCCGTTGCTCAGGCA
CAGGCACTGTACGAAAGCGCTCCCGGTCTCTGCCTGCATGTGGTTTCCGTTGCCGAAACTGAAGCAGGCAAAGCCGTGCT
TGACCGTATTGCCGCGCTGAAAGGCTGCTCCGTAACTGCCGATGCCGCAACCCTGCTGGCTGATGAGCAGGCACTGAACG
GCTTTGTCAAAGACGTCTTCTACGACACCCGTGAAGTGAAAAAGGCACCTGCCCCCGCTCCGGTTGTCACCCCCGAAACT
GAAGAAGTCATCGTTCTGCGCAGTGTCCAGTTTGCTTTTGACTCTGCAGAAATCAGCAATGAGGCCGAAGATGTGCTGAC
CGAAGCCGCAACGCTGATCGAAGAACATGCCGGCAAGCAGGTTACTCTTGCAGGTCACACCGACAGCACCGGCCCCGAAG
CGTACAACCAGAAGCTCTCTCAGCGCCGAGCTGAATCGGTTAAGGCATTTCTTGCCAAAGAGGGAGTTGACGCCAATCGC
ATCAAGGCCGTAGGATTCGGCGAAAGCGAACCCAAGTTCGACAACAATACCGCCGAAGGAAGAAAGCTGAACAGAAGGGT
TGAAATCACCTTCAAGTAA

Upstream 100 bases:

>100_bases
TTTCTTTTAGTGTTGTCACAATAAAGTATTCGCGCTATGTTTTTCACCATCAGTATCGGCCGTAACTAATTTTTTCATTA
TTCCATCTGGAGGTATAGTT

Downstream 100 bases:

>100_bases
GGCTTGTAATCGCTAATAAAAACAGCCCGGCGGAATCCCGCCGGGCTGTTTTGCATCAACACGTTTGCCGGAGACGACAG
CAACATGAAACTGTTCATGG

Product: OmpA family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MKLTLKPVFMFLLAMLIMAPAFSHAADMEYIPKIASFDFFVDDSGSMMMTHQKLEKPKIEVAKDVLSKINNAIPALKYNA
SMHMFAPYEEVVPYGPYSKSGFEQGIMSLKADKEIFARRTPMGDGLNMLRPAADNMARNAAVIIVSDGESNFGSDPVAQA
QALYESAPGLCLHVVSVAETEAGKAVLDRIAALKGCSVTADAATLLADEQALNGFVKDVFYDTREVKKAPAPAPVVTPET
EEVIVLRSVQFAFDSAEISNEAEDVLTEAATLIEEHAGKQVTLAGHTDSTGPEAYNQKLSQRRAESVKAFLAKEGVDANR
IKAVGFGESEPKFDNNTAEGRKLNRRVEITFK

Sequences:

>Translated_352_residues
MKLTLKPVFMFLLAMLIMAPAFSHAADMEYIPKIASFDFFVDDSGSMMMTHQKLEKPKIEVAKDVLSKINNAIPALKYNA
SMHMFAPYEEVVPYGPYSKSGFEQGIMSLKADKEIFARRTPMGDGLNMLRPAADNMARNAAVIIVSDGESNFGSDPVAQA
QALYESAPGLCLHVVSVAETEAGKAVLDRIAALKGCSVTADAATLLADEQALNGFVKDVFYDTREVKKAPAPAPVVTPET
EEVIVLRSVQFAFDSAEISNEAEDVLTEAATLIEEHAGKQVTLAGHTDSTGPEAYNQKLSQRRAESVKAFLAKEGVDANR
IKAVGFGESEPKFDNNTAEGRKLNRRVEITFK
>Mature_352_residues
MKLTLKPVFMFLLAMLIMAPAFSHAADMEYIPKIASFDFFVDDSGSMMMTHQKLEKPKIEVAKDVLSKINNAIPALKYNA
SMHMFAPYEEVVPYGPYSKSGFEQGIMSLKADKEIFARRTPMGDGLNMLRPAADNMARNAAVIIVSDGESNFGSDPVAQA
QALYESAPGLCLHVVSVAETEAGKAVLDRIAALKGCSVTADAATLLADEQALNGFVKDVFYDTREVKKAPAPAPVVTPET
EEVIVLRSVQFAFDSAEISNEAEDVLTEAATLIEEHAGKQVTLAGHTDSTGPEAYNQKLSQRRAESVKAFLAKEGVDANR
IKAVGFGESEPKFDNNTAEGRKLNRRVEITFK

Specific function: Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium [H]

COG id: COG2885

COG function: function code M; Outer membrane protein and related peptidoglycan-associated (lipo)proteins

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OmpA-like domain [H]

Homologues:

Organism=Escherichia coli, GI48994946, Length=137, Percent_Identity=37.956204379562, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787191, Length=127, Percent_Identity=35.4330708661417, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011250
- InterPro:   IPR006664
- InterPro:   IPR006690
- InterPro:   IPR006665
- InterPro:   IPR008722 [H]

Pfam domain/function: PF00691 OmpA; PF05736 OprF [H]

EC number: NA

Molecular weight: Translated: 38247; Mature: 38247

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS50234 VWFA ; PS01068 OMPA_1 ; PS51123 OMPA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLTLKPVFMFLLAMLIMAPAFSHAADMEYIPKIASFDFFVDDSGSMMMTHQKLEKPKIE
CCEEHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHCCEEEECCCCCEEEEHHHHCCCHHH
VAKDVLSKINNAIPALKYNASMHMFAPYEEVVPYGPYSKSGFEQGIMSLKADKEIFARRT
HHHHHHHHHHHHCCCEEECCCEEEECCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
PMGDGLNMLRPAADNMARNAAVIIVSDGESNFGSDPVAQAQALYESAPGLCLHVVSVAET
CCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCEEHHHHHHHH
EAGKAVLDRIAALKGCSVTADAATLLADEQALNGFVKDVFYDTREVKKAPAPAPVVTPET
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
EEVIVLRSVQFAFDSAEISNEAEDVLTEAATLIEEHAGKQVTLAGHTDSTGPEAYNQKLS
CCEEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHH
QRRAESVKAFLAKEGVDANRIKAVGFGESEPKFDNNTAEGRKLNRRVEITFK
HHHHHHHHHHHHHCCCCHHHEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEC
>Mature Secondary Structure
MKLTLKPVFMFLLAMLIMAPAFSHAADMEYIPKIASFDFFVDDSGSMMMTHQKLEKPKIE
CCEEHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHCCEEEECCCCCEEEEHHHHCCCHHH
VAKDVLSKINNAIPALKYNASMHMFAPYEEVVPYGPYSKSGFEQGIMSLKADKEIFARRT
HHHHHHHHHHHHCCCEEECCCEEEECCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
PMGDGLNMLRPAADNMARNAAVIIVSDGESNFGSDPVAQAQALYESAPGLCLHVVSVAET
CCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCEEHHHHHHHH
EAGKAVLDRIAALKGCSVTADAATLLADEQALNGFVKDVFYDTREVKKAPAPAPVVTPET
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
EEVIVLRSVQFAFDSAEISNEAEDVLTEAATLIEEHAGKQVTLAGHTDSTGPEAYNQKLS
CCEEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHH
QRRAESVKAFLAKEGVDANRIKAVGFGESEPKFDNNTAEGRKLNRRVEITFK
HHHHHHHHHHHHHCCCCHHHEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2447060; 10984043 [H]