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The map label for this gene is thiC [H]

Identifier: 78356723

GI number: 78356723

Start: 1730458

End: 1731747

Strand: Direct

Name: thiC [H]

Synonym: Dde_1680

Alternate gene names: 78356723

Gene position: 1730458-1731747 (Clockwise)

Preceding gene: 78356721

Following gene: 78356725

Centisome position: 46.39

GC content: 56.98

Gene sequence:

>1290_bases
ATGTCCATTCTTGAAGCAAACACCGCTCTCCGGAGCCTTCTCGACCAGCACATTTCCATGCTCGCGGAAAAAGAACATAT
TTCCGCAGAGACAATCCGTGCCGGTATAGAAAACGGCACTATGGTGCTGCTCGGTAACCCTTCACACGGCAATGTACTGC
CTACGCTGGTCGGCCAGCCTGCCGGTGTAAAGGTGAACGCCAACATCGGCACGTCTCCTCTGAAAAACAGTGTGGCCTGC
GAAATGCGCAAGCTGCAGGTGGCCGAACAGGCCGGCGCGGATACCGTCATGGATCTTTCCATTGCGGGAGATCTGGACGC
CATCAGACGCGAAATGCTTTCGCAGACCAGATTGCCTCTGGGCACGGTACCTATGTATTCCGTGGCTCAGCGGTATCTTG
ATGCAGATCGCGATCCTGCTGAAATGGATCCTGAAGAGCTTTTCGAAGAAATCGAAAAGCAGGCCGGGCAGGGTGTCGAT
TTTATTACAGTGCACTGCGGGCTTACCCGTCGCGGAGCCGAACTGGCTACCGACGGCAGCCGTCTGCTGGGTATAGTTTC
GCGGGGCGGTTCCATTCTTGCGCGCTGGATGCTGCGTAACAACAAAGAAAACCCTCTGCTTACCGGATTTGACCGTCTGA
TCGACATCTGCCGCAGACATAACGTGACGTTTTCTCTGGGTGACGGTCTGCGCCCCGGCGCCGGTCATGATGCGGGGGAC
GCGGCCCAGTACGAGGAAGTCATGGTGCTGGGGCAGCTGGCCAAAAGAGCTCTTGCCGCCGGTGCGCAGTGCATGATTGA
AGGTCCCGGCCATGTGCCGTTCAATCAGGTACGCAGCCAGATTCAGACTATCAAGCGGATGACTAACAATGCCCCGTTGT
ATGTGCTGGGGCCGCTGACCATTGATTCCTGCCCCGGCTACGACCATATTGCAGGTGCCATCGGCGGAGCCATTGCTGTG
GAGGCAGGGGTTGACTTCCTCTGCTATCTTACTCCCGCCGAGCATCTGACTTTGCCCACCATGGATGATGTCCATGCGGG
CGTTGTGGCTTCACGCATCGCCGCACAGGCTGGTGAAGTTGCTCTTGGCAGGGCTGCCGCGCTGGAACGCGAAGAGGGTA
TTTCCCGCGCGCGTAAGGACCTCGACTGGGATGCCATGAAAAAATATGCTCTGGATGCGCCTAAAATCGATGAGCGTCGT
GGCGAGCATAAAGACGAGGAAGAGTGCGCCATGTGCGGTAAGTTCTGCGCGGTGAAAATGCTGCGCGAAGACCCCATGGG
CAAAATTTAG

Upstream 100 bases:

>100_bases
TTTTTCTGTTGCCAACGCGGCGGTGCAAAGCTATGTTCCGGACGTTGTGTGTGTTCCCTGGCCCCTCCCGCAGTGCTTAC
CAAGCCTGGGAGATAATTTT

Downstream 100 bases:

>100_bases
CCCTTTCAAACCGTAACGACACTAGAAGGCGGGGTGCTGCGGCACCCCGCCTTTCTTTCTGATCACAGCATGTGCGCGTT
GACCACATTTTGCGGTGTGC

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC [H]

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MSILEANTALRSLLDQHISMLAEKEHISAETIRAGIENGTMVLLGNPSHGNVLPTLVGQPAGVKVNANIGTSPLKNSVAC
EMRKLQVAEQAGADTVMDLSIAGDLDAIRREMLSQTRLPLGTVPMYSVAQRYLDADRDPAEMDPEELFEEIEKQAGQGVD
FITVHCGLTRRGAELATDGSRLLGIVSRGGSILARWMLRNNKENPLLTGFDRLIDICRRHNVTFSLGDGLRPGAGHDAGD
AAQYEEVMVLGQLAKRALAAGAQCMIEGPGHVPFNQVRSQIQTIKRMTNNAPLYVLGPLTIDSCPGYDHIAGAIGGAIAV
EAGVDFLCYLTPAEHLTLPTMDDVHAGVVASRIAAQAGEVALGRAAALEREEGISRARKDLDWDAMKKYALDAPKIDERR
GEHKDEEECAMCGKFCAVKMLREDPMGKI

Sequences:

>Translated_429_residues
MSILEANTALRSLLDQHISMLAEKEHISAETIRAGIENGTMVLLGNPSHGNVLPTLVGQPAGVKVNANIGTSPLKNSVAC
EMRKLQVAEQAGADTVMDLSIAGDLDAIRREMLSQTRLPLGTVPMYSVAQRYLDADRDPAEMDPEELFEEIEKQAGQGVD
FITVHCGLTRRGAELATDGSRLLGIVSRGGSILARWMLRNNKENPLLTGFDRLIDICRRHNVTFSLGDGLRPGAGHDAGD
AAQYEEVMVLGQLAKRALAAGAQCMIEGPGHVPFNQVRSQIQTIKRMTNNAPLYVLGPLTIDSCPGYDHIAGAIGGAIAV
EAGVDFLCYLTPAEHLTLPTMDDVHAGVVASRIAAQAGEVALGRAAALEREEGISRARKDLDWDAMKKYALDAPKIDERR
GEHKDEEECAMCGKFCAVKMLREDPMGKI
>Mature_428_residues
SILEANTALRSLLDQHISMLAEKEHISAETIRAGIENGTMVLLGNPSHGNVLPTLVGQPAGVKVNANIGTSPLKNSVACE
MRKLQVAEQAGADTVMDLSIAGDLDAIRREMLSQTRLPLGTVPMYSVAQRYLDADRDPAEMDPEELFEEIEKQAGQGVDF
ITVHCGLTRRGAELATDGSRLLGIVSRGGSILARWMLRNNKENPLLTGFDRLIDICRRHNVTFSLGDGLRPGAGHDAGDA
AQYEEVMVLGQLAKRALAAGAQCMIEGPGHVPFNQVRSQIQTIKRMTNNAPLYVLGPLTIDSCPGYDHIAGAIGGAIAVE
AGVDFLCYLTPAEHLTLPTMDDVHAGVVASRIAAQAGEVALGRAAALEREEGISRARKDLDWDAMKKYALDAPKIDERRG
EHKDEEECAMCGKFCAVKMLREDPMGKI

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=406, Percent_Identity=39.6551724137931, Blast_Score=293, Evalue=1e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002817 [H]

Pfam domain/function: PF01964 ThiC [H]

EC number: NA

Molecular weight: Translated: 46239; Mature: 46107

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSILEANTALRSLLDQHISMLAEKEHISAETIRAGIENGTMVLLGNPSHGNVLPTLVGQP
CCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEEEECCCCCCCCHHHCCCC
AGVKVNANIGTSPLKNSVACEMRKLQVAEQAGADTVMDLSIAGDLDAIRREMLSQTRLPL
CCEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHCCCC
GTVPMYSVAQRYLDADRDPAEMDPEELFEEIEKQAGQGVDFITVHCGLTRRGAELATDGS
CCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHCCCH
RLLGIVSRGGSILARWMLRNNKENPLLTGFDRLIDICRRHNVTFSLGDGLRPGAGHDAGD
HHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCC
AAQYEEVMVLGQLAKRALAAGAQCMIEGPGHVPFNQVRSQIQTIKRMTNNAPLYVLGPLT
HHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEECCEE
IDSCPGYDHIAGAIGGAIAVEAGVDFLCYLTPAEHLTLPTMDDVHAGVVASRIAAQAGEV
ECCCCCHHHHHHHHCCHHEECCCCCEEEEECCHHHEECCCHHHHHHHHHHHHHHHHCCCH
ALGRAAALEREEGISRARKDLDWDAMKKYALDAPKIDERRGEHKDEEECAMCGKFCAVKM
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHCCCCCCHHHHHHHHHHHHHHH
LREDPMGKI
HHCCCCCCC
>Mature Secondary Structure 
SILEANTALRSLLDQHISMLAEKEHISAETIRAGIENGTMVLLGNPSHGNVLPTLVGQP
CCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEEEECCCCCCCCHHHCCCC
AGVKVNANIGTSPLKNSVACEMRKLQVAEQAGADTVMDLSIAGDLDAIRREMLSQTRLPL
CCEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHCCCC
GTVPMYSVAQRYLDADRDPAEMDPEELFEEIEKQAGQGVDFITVHCGLTRRGAELATDGS
CCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHCCCH
RLLGIVSRGGSILARWMLRNNKENPLLTGFDRLIDICRRHNVTFSLGDGLRPGAGHDAGD
HHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCC
AAQYEEVMVLGQLAKRALAAGAQCMIEGPGHVPFNQVRSQIQTIKRMTNNAPLYVLGPLT
HHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEECCEE
IDSCPGYDHIAGAIGGAIAVEAGVDFLCYLTPAEHLTLPTMDDVHAGVVASRIAAQAGEV
ECCCCCHHHHHHHHCCHHEECCCCCEEEEECCHHHEECCCHHHHHHHHHHHHHHHHCCCH
ALGRAAALEREEGISRARKDLDWDAMKKYALDAPKIDERRGEHKDEEECAMCGKFCAVKM
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHCCCCCCHHHHHHHHHHHHHHH
LREDPMGKI
HHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]