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The map label for this gene is rfaF [H]

Identifier: 78356672

GI number: 78356672

Start: 1680074

End: 1681099

Strand: Direct

Name: rfaF [H]

Synonym: Dde_1629

Alternate gene names: 78356672

Gene position: 1680074-1681099 (Clockwise)

Preceding gene: 78356670

Following gene: 78356674

Centisome position: 45.04

GC content: 62.87

Gene sequence:

>1026_bases
ATGAATGAACCTCAGAAATGGCTGGTTGTAAGGCTCAGCGCTCTTGGTGACGTTGTGCTTACCACCGGCGTGCTCGACTA
CCTGTCCCGGCACCGCGGGTGGCGGTTCCACGTTCTGACGCGCAGGGCCTGGGCACCTGTATTTGACGGGCTGCCCGCCG
TAGAAGGGGTTGCCCCTCTGGATGACGCGGATTTGCACATGCCGCGCATGGCCGGGGTACTGCGACGCCTTGCGCGACAG
TATGAAGGCTGGGGGCTGCTTGACCTGCACGGCACTTTGCGGTCGCGGCTGCTTGGTTCCCTGTGGCGGGGACCTGTGCG
CAGGTATCCTAAAATGGCGGTCTGCCGCCGGCTTTTTCTGGCCAGCGGCGGGCGTTTATGCGCTGCAGCACTGGAGCAGT
TCAATGTTCCGCAGCGGTATGCTCTGGCAGTGGAAAAAAAGGCGCCGCCCAGAAATGCGCTGCTGCCCGTCATCAGGCTT
GATGAGGCCGAAACTGCAGCTGCCGGCGGCCTGCTGCCGTCTGCACCTGACAGGCCTTTGGTGGCGCTGCACCCGTACTC
AACCCATTCGCACAAGGCATGGCTGGACGACAACTGGCGCGAGCTGGCGGTGGGACTGGTGCATAGCGGACTGCGCTGTG
TGGTTGTGGGGGTGGGGCAGTCGCCTTTTGCAGGTATGCAGCTTCCGGAGGCATACTTTGCCGACTGTACGGGGCGTACG
TCCCTGCGGCAGAGCTGTGCGCTGCTGGCCGCTGCCGATGTGCTGGTAACGGGTGATTCCGGCCCCATGCATATGGCCTG
CGGAACAGGCACTCCTGTGGTGGCTCTGTTCGGGCCCACACACCGTGCATGGGGGTTTTATCCCGAGGGTCCCCGTGATG
TGGTGCTTGAAGCCGATATGGCGTGCAGGCCCTGTTCGCTGCACGGCAGCAAACCCTGTGCCCATGGTCAGCGCTGCATG
CAGAGCATCAGTGTTGACGATGTGAGGCAGGGGATACGGCAGGTGCTGAACAGTGCAGCGCTTTAG

Upstream 100 bases:

>100_bases
GCAGTGCGGCTTTGACTTTTCTTTCGGGCTGCCACCTGCTACGGTCGCGCCCTTGCGGCCGGTTACTGTACAATACGCCG
TCCGCGTAAAGGAATGCGGG

Downstream 100 bases:

>100_bases
CGCGAAAAGAGTTTTTTTTCTGCCTGCCGGACAATGTCGGAGGCTTCAGCAAGTTGCGGGTCGCGTTCCAGAGCGAGCCG
TGCATAATCATAGGCCAGAT

Product: heptosyltransferase family protein

Products: NA

Alternate protein names: ADP-heptose--LPS heptosyltransferase II [H]

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MNEPQKWLVVRLSALGDVVLTTGVLDYLSRHRGWRFHVLTRRAWAPVFDGLPAVEGVAPLDDADLHMPRMAGVLRRLARQ
YEGWGLLDLHGTLRSRLLGSLWRGPVRRYPKMAVCRRLFLASGGRLCAAALEQFNVPQRYALAVEKKAPPRNALLPVIRL
DEAETAAAGGLLPSAPDRPLVALHPYSTHSHKAWLDDNWRELAVGLVHSGLRCVVVGVGQSPFAGMQLPEAYFADCTGRT
SLRQSCALLAAADVLVTGDSGPMHMACGTGTPVVALFGPTHRAWGFYPEGPRDVVLEADMACRPCSLHGSKPCAHGQRCM
QSISVDDVRQGIRQVLNSAAL

Sequences:

>Translated_341_residues
MNEPQKWLVVRLSALGDVVLTTGVLDYLSRHRGWRFHVLTRRAWAPVFDGLPAVEGVAPLDDADLHMPRMAGVLRRLARQ
YEGWGLLDLHGTLRSRLLGSLWRGPVRRYPKMAVCRRLFLASGGRLCAAALEQFNVPQRYALAVEKKAPPRNALLPVIRL
DEAETAAAGGLLPSAPDRPLVALHPYSTHSHKAWLDDNWRELAVGLVHSGLRCVVVGVGQSPFAGMQLPEAYFADCTGRT
SLRQSCALLAAADVLVTGDSGPMHMACGTGTPVVALFGPTHRAWGFYPEGPRDVVLEADMACRPCSLHGSKPCAHGQRCM
QSISVDDVRQGIRQVLNSAAL
>Mature_341_residues
MNEPQKWLVVRLSALGDVVLTTGVLDYLSRHRGWRFHVLTRRAWAPVFDGLPAVEGVAPLDDADLHMPRMAGVLRRLARQ
YEGWGLLDLHGTLRSRLLGSLWRGPVRRYPKMAVCRRLFLASGGRLCAAALEQFNVPQRYALAVEKKAPPRNALLPVIRL
DEAETAAAGGLLPSAPDRPLVALHPYSTHSHKAWLDDNWRELAVGLVHSGLRCVVVGVGQSPFAGMQLPEAYFADCTGRT
SLRQSCALLAAADVLVTGDSGPMHMACGTGTPVVALFGPTHRAWGFYPEGPRDVVLEADMACRPCSLHGSKPCAHGQRCM
QSISVDDVRQGIRQVLNSAAL

Specific function: Lipopolysaccharide core biosynthesis. [C]

COG id: COG0859

COG function: function code M; ADP-heptose:LPS heptosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 9 family [H]

Homologues:

Organism=Escherichia coli, GI1790050, Length=139, Percent_Identity=29.4964028776978, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002201
- InterPro:   IPR011910 [H]

Pfam domain/function: PF01075 Glyco_transf_9 [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 37152; Mature: 37152

Theoretical pI: Translated: 9.02; Mature: 9.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEPQKWLVVRLSALGDVVLTTGVLDYLSRHRGWRFHVLTRRAWAPVFDGLPAVEGVAPL
CCCCHHHHHEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHCCCCCCCCCCCC
DDADLHMPRMAGVLRRLARQYEGWGLLDLHGTLRSRLLGSLWRGPVRRYPKMAVCRRLFL
CCCCCCHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
ASGGRLCAAALEQFNVPQRYALAVEKKAPPRNALLPVIRLDEAETAAAGGLLPSAPDRPL
HCCCHHHHHHHHHCCCCHHHEEEEECCCCCCCCCCCEEEECCCHHHHHCCCCCCCCCCCE
VALHPYSTHSHKAWLDDNWRELAVGLVHSGLRCVVVGVGQSPFAGMQLPEAYFADCTGRT
EEECCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHCCCCCH
SLRQSCALLAAADVLVTGDSGPMHMACGTGTPVVALFGPTHRAWGFYPEGPRDVVLEADM
HHHHHHHHHHHEEEEEECCCCCEEEEECCCCCEEEEECCCCCCCCCCCCCCCCEEEECCC
ACRPCSLHGSKPCAHGQRCMQSISVDDVRQGIRQVLNSAAL
CCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNEPQKWLVVRLSALGDVVLTTGVLDYLSRHRGWRFHVLTRRAWAPVFDGLPAVEGVAPL
CCCCHHHHHEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHCCCCCCCCCCCC
DDADLHMPRMAGVLRRLARQYEGWGLLDLHGTLRSRLLGSLWRGPVRRYPKMAVCRRLFL
CCCCCCHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
ASGGRLCAAALEQFNVPQRYALAVEKKAPPRNALLPVIRLDEAETAAAGGLLPSAPDRPL
HCCCHHHHHHHHHCCCCHHHEEEEECCCCCCCCCCCEEEECCCHHHHHCCCCCCCCCCCE
VALHPYSTHSHKAWLDDNWRELAVGLVHSGLRCVVVGVGQSPFAGMQLPEAYFADCTGRT
EEECCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHCCCCCH
SLRQSCALLAAADVLVTGDSGPMHMACGTGTPVVALFGPTHRAWGFYPEGPRDVVLEADM
HHHHHHHHHHHEEEEEECCCCCEEEEECCCCCEEEEECCCCCCCCCCCCCCCCEEEECCC
ACRPCSLHGSKPCAHGQRCMQSISVDDVRQGIRQVLNSAAL
CCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503; 2198271; 8478319 [H]