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The map label for this gene is metG [H]

Identifier: 78356637

GI number: 78356637

Start: 1622731

End: 1624659

Strand: Direct

Name: metG [H]

Synonym: Dde_1594

Alternate gene names: 78356637

Gene position: 1622731-1624659 (Clockwise)

Preceding gene: 78356636

Following gene: 78356649

Centisome position: 43.5

GC content: 55.0

Gene sequence:

>1929_bases
TTGCAACCTTTTTACATCACGACCCCCATTTACTACGTTAACGCCAAGCCCCATCTGGGACATGCATATACGACCATCGT
GGCTGATGCGCTGCGCCGTTTTCATACGCTGCTGGGCAAGAAAACCTTTTTTCTGACCGGTACCGACGAACACGGGGATA
AAATAGTGCAGGCTGCCGAAAAAAACGGCTGCACCCCGCAGGAATATGTGGACTCCATAAGTGCCACTTTCCGTGACCTG
TGGCCCTCCATGGGCATTACCTATGACCGTTTTGTCCGCACCACCGATGAATCGCATAAAAAATGCGTACAGGCCGTCCT
GCAGAAGGTGTACGACAGCGGAGATATTTATTTCGGCGAATATGGCGGGCACTACTGTTACGGGTGTGAACGTTTTTATA
CCGAAAAAGAGCTGGAGAACGGACTTTGTCCGCAGCATCAGGTCAAGCCGGAATACATTGCCGAAAAGAACTACTTCTTC
AGAATGTCCAAATATCAGGACTGGCTGAGGCAGTATATTCTGGACAATCCCGATTTTATCCGCCCCGAGCGGTATCGTAA
CGAAGTGCTTTCGCTGCTGGACAGCGGCGCGCTTGAAGACCTGTGCATTTCCCGTCCCAAAAGCCGCCTTTCATGGGGGG
TGGAACTGCCGTTTGACACGGATTACGTGTGTTACGTGTGGTTTGACGCGCTGCTGAACTATGTGTCGGCGCTTAACTGG
CCCGACGGAGAAGAGTTTACGACGTTCTGGCCCGGAGTGCAGCATCTGGTGGCCAAGGACATTCTGAAGCCCCATGCCGT
GTTCTGGCCCACCATGCTTAAAGCGGCCGGGTTTGAACCGTACAACAACCTGAATGTGCACGGTTACTGGCTGGTGCGCG
ACACCAAAATGTCCAAGTCGCTCGGTAATGTCATAGAGCCTCTTGAAATGGCCGGCCGGTACGGGCTGAGTGCTTTCCGG
TATTTTCTGCTGCGCGAGATGCATTTCGGCAGCGACGCCAGTTTTTCCGAAGAGGCACTGGTAGCCCGTCTTAATGCTGA
TCTGGCCAATGATCTCGGCAACCTGTTCAGCCGTGTGCTTTCCATGACAGGTAAGTACTTTGACGGTGTGGTACCTTCTC
CTGCCGCATATACTGCGGATGATGAAGATATCATCAATCTGGCCAAGGATGCCTGCCGCAACTATGTCCGCCTGTTTGAA
CGCGTGCGCTTTTCCAATGCGCTGGAATCATTGTGGGAGCTTGTGCGCGCGCTGAACAAGTATGTGGACAGCAGCGCCCC
GTGGACGCTTTATAAAACCGGTGACACGGCCCGGCTGGGCACTGTGCTGTACGTGCTGCTGGAATGCATGCGGAAAGTCG
CTGTCAATCTGTGGCCTGTCATGCCCGAAGCGTCCGAAGCAATGCTGGGTCAGCTGCATGTATGCTTTGACTCTGCCGGT
ACCAGGCTGCAGGATGAAGCCGAAGCCTGGGGCACATTGCAGGCGGGTGGCAAGGTCGAAGTTTCTTCCGGTCTTTTCCC
GCGGGTGGAGGTGGCAAAGGAAAAAGACCGCCCTGCCGAGCAGCCGGTTCCGAAAGCACAGCCAGTGGCAGTGCCTGCTG
TCGAGTATGCCGAGTTCGAAGACTTTCAGAAGGTCGAGCTGCGCGTGGGCACCGTGCTGACAGTTGAAAATCATCCCAAG
GCGGATAAGTTGTACCGCATCGAGGTCGATCTGGGCGAAGAGCAGCCCAGACAGATTATCGCCGGTTTGAAAGAATTCTT
TGCCCCCGATGCGCTGACGGGACGTCAGGTGGTCGTTGTGGCCAACCTGAAGCCGCGCAAACTGCGCGGGTTGGAGTCGC
AGGGGATGATTCTGGCGTTGCGTACGGAAGACGGCATGGAGTTGCTTACGGCATCCAATCCTGTTCCTGCCGGTACGCGG
GCTTCTTGA

Upstream 100 bases:

>100_bases
GGCAAGCGTAAACGCAAACGCCGTACCAGAACCGACGAATACGATCAGAACGACTAACCCCGAACGATCATATCGCCGTT
GCAAAGACGAGGAGTAGACG

Downstream 100 bases:

>100_bases
CGGTTCAGACCTGCAGAGGATAAAAATACAAACGCCGCCTGAGAGATGCTCGGGCGGCGTTTTTTTGTCGGCAAAGCCGG
AAAGTATGCAAGTTGGTGCA

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 642; Mature: 642

Protein sequence:

>642_residues
MQPFYITTPIYYVNAKPHLGHAYTTIVADALRRFHTLLGKKTFFLTGTDEHGDKIVQAAEKNGCTPQEYVDSISATFRDL
WPSMGITYDRFVRTTDESHKKCVQAVLQKVYDSGDIYFGEYGGHYCYGCERFYTEKELENGLCPQHQVKPEYIAEKNYFF
RMSKYQDWLRQYILDNPDFIRPERYRNEVLSLLDSGALEDLCISRPKSRLSWGVELPFDTDYVCYVWFDALLNYVSALNW
PDGEEFTTFWPGVQHLVAKDILKPHAVFWPTMLKAAGFEPYNNLNVHGYWLVRDTKMSKSLGNVIEPLEMAGRYGLSAFR
YFLLREMHFGSDASFSEEALVARLNADLANDLGNLFSRVLSMTGKYFDGVVPSPAAYTADDEDIINLAKDACRNYVRLFE
RVRFSNALESLWELVRALNKYVDSSAPWTLYKTGDTARLGTVLYVLLECMRKVAVNLWPVMPEASEAMLGQLHVCFDSAG
TRLQDEAEAWGTLQAGGKVEVSSGLFPRVEVAKEKDRPAEQPVPKAQPVAVPAVEYAEFEDFQKVELRVGTVLTVENHPK
ADKLYRIEVDLGEEQPRQIIAGLKEFFAPDALTGRQVVVVANLKPRKLRGLESQGMILALRTEDGMELLTASNPVPAGTR
AS

Sequences:

>Translated_642_residues
MQPFYITTPIYYVNAKPHLGHAYTTIVADALRRFHTLLGKKTFFLTGTDEHGDKIVQAAEKNGCTPQEYVDSISATFRDL
WPSMGITYDRFVRTTDESHKKCVQAVLQKVYDSGDIYFGEYGGHYCYGCERFYTEKELENGLCPQHQVKPEYIAEKNYFF
RMSKYQDWLRQYILDNPDFIRPERYRNEVLSLLDSGALEDLCISRPKSRLSWGVELPFDTDYVCYVWFDALLNYVSALNW
PDGEEFTTFWPGVQHLVAKDILKPHAVFWPTMLKAAGFEPYNNLNVHGYWLVRDTKMSKSLGNVIEPLEMAGRYGLSAFR
YFLLREMHFGSDASFSEEALVARLNADLANDLGNLFSRVLSMTGKYFDGVVPSPAAYTADDEDIINLAKDACRNYVRLFE
RVRFSNALESLWELVRALNKYVDSSAPWTLYKTGDTARLGTVLYVLLECMRKVAVNLWPVMPEASEAMLGQLHVCFDSAG
TRLQDEAEAWGTLQAGGKVEVSSGLFPRVEVAKEKDRPAEQPVPKAQPVAVPAVEYAEFEDFQKVELRVGTVLTVENHPK
ADKLYRIEVDLGEEQPRQIIAGLKEFFAPDALTGRQVVVVANLKPRKLRGLESQGMILALRTEDGMELLTASNPVPAGTR
AS
>Mature_642_residues
MQPFYITTPIYYVNAKPHLGHAYTTIVADALRRFHTLLGKKTFFLTGTDEHGDKIVQAAEKNGCTPQEYVDSISATFRDL
WPSMGITYDRFVRTTDESHKKCVQAVLQKVYDSGDIYFGEYGGHYCYGCERFYTEKELENGLCPQHQVKPEYIAEKNYFF
RMSKYQDWLRQYILDNPDFIRPERYRNEVLSLLDSGALEDLCISRPKSRLSWGVELPFDTDYVCYVWFDALLNYVSALNW
PDGEEFTTFWPGVQHLVAKDILKPHAVFWPTMLKAAGFEPYNNLNVHGYWLVRDTKMSKSLGNVIEPLEMAGRYGLSAFR
YFLLREMHFGSDASFSEEALVARLNADLANDLGNLFSRVLSMTGKYFDGVVPSPAAYTADDEDIINLAKDACRNYVRLFE
RVRFSNALESLWELVRALNKYVDSSAPWTLYKTGDTARLGTVLYVLLECMRKVAVNLWPVMPEASEAMLGQLHVCFDSAG
TRLQDEAEAWGTLQAGGKVEVSSGLFPRVEVAKEKDRPAEQPVPKAQPVAVPAVEYAEFEDFQKVELRVGTVLTVENHPK
ADKLYRIEVDLGEEQPRQIIAGLKEFFAPDALTGRQVVVVANLKPRKLRGLESQGMILALRTEDGMELLTASNPVPAGTR
AS

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=498, Percent_Identity=37.7510040160643, Blast_Score=320, Evalue=2e-87,
Organism=Homo sapiens, GI14043022, Length=555, Percent_Identity=28.4684684684685, Blast_Score=173, Evalue=4e-43,
Organism=Homo sapiens, GI4507947, Length=117, Percent_Identity=41.025641025641, Blast_Score=92, Evalue=2e-18,
Organism=Homo sapiens, GI215490011, Length=102, Percent_Identity=36.2745098039216, Blast_Score=81, Evalue=3e-15,
Organism=Homo sapiens, GI215490009, Length=100, Percent_Identity=37, Blast_Score=81, Evalue=4e-15,
Organism=Homo sapiens, GI45006986, Length=100, Percent_Identity=37, Blast_Score=81, Evalue=4e-15,
Organism=Escherichia coli, GI1788432, Length=673, Percent_Identity=28.3803863298663, Blast_Score=229, Evalue=5e-61,
Organism=Escherichia coli, GI1789455, Length=110, Percent_Identity=39.0909090909091, Blast_Score=84, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI212645096, Length=520, Percent_Identity=33.6538461538462, Blast_Score=288, Evalue=7e-78,
Organism=Caenorhabditis elegans, GI17540794, Length=702, Percent_Identity=25.0712250712251, Blast_Score=198, Evalue=8e-51,
Organism=Caenorhabditis elegans, GI71992781, Length=412, Percent_Identity=31.5533980582524, Blast_Score=194, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6321610, Length=521, Percent_Identity=33.7811900191939, Blast_Score=274, Evalue=3e-74,
Organism=Saccharomyces cerevisiae, GI6321704, Length=549, Percent_Identity=22.7686703096539, Blast_Score=125, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6321333, Length=97, Percent_Identity=38.1443298969072, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21358483, Length=491, Percent_Identity=37.2708757637475, Blast_Score=318, Evalue=6e-87,
Organism=Drosophila melanogaster, GI24655311, Length=516, Percent_Identity=26.937984496124, Blast_Score=158, Evalue=1e-38,
Organism=Drosophila melanogaster, GI21357223, Length=102, Percent_Identity=42.156862745098, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24651924, Length=99, Percent_Identity=39.3939393939394, Blast_Score=81, Evalue=3e-15,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080 [H]

Pfam domain/function: PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 72813; Mature: 72813

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPFYITTPIYYVNAKPHLGHAYTTIVADALRRFHTLLGKKTFFLTGTDEHGDKIVQAAE
CCCEEEECCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHH
KNGCTPQEYVDSISATFRDLWPSMGITYDRFVRTTDESHKKCVQAVLQKVYDSGDIYFGE
HCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEC
YGGHYCYGCERFYTEKELENGLCPQHQVKPEYIAEKNYFFRMSKYQDWLRQYILDNPDFI
CCCEEEECHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCEEEHHHHHHHHHHHHCCCCCCC
RPERYRNEVLSLLDSGALEDLCISRPKSRLSWGVELPFDTDYVCYVWFDALLNYVSALNW
CCHHHHHHHHHHHHCCCHHHHHHCCCHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHCCC
PDGEEFTTFWPGVQHLVAKDILKPHAVFWPTMLKAAGFEPYNNLNVHGYWLVRDTKMSKS
CCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCHHHHH
LGNVIEPLEMAGRYGLSAFRYFLLREMHFGSDASFSEEALVARLNADLANDLGNLFSRVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SMTGKYFDGVVPSPAAYTADDEDIINLAKDACRNYVRLFERVRFSNALESLWELVRALNK
HHCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YVDSSAPWTLYKTGDTARLGTVLYVLLECMRKVAVNLWPVMPEASEAMLGQLHVCFDSAG
HHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHEECCCC
TRLQDEAEAWGTLQAGGKVEVSSGLFPRVEVAKEKDRPAEQPVPKAQPVAVPAVEYAEFE
CCCHHHHHHCCEECCCCEEEECCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHH
DFQKVELRVGTVLTVENHPKADKLYRIEVDLGEEQPRQIIAGLKEFFAPDALTGRQVVVV
HHHHHEEEEEEEEEECCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCEEEEE
ANLKPRKLRGLESQGMILALRTEDGMELLTASNPVPAGTRAS
ECCCCHHHCCCCCCCEEEEEECCCCCEEEECCCCCCCCCCCC
>Mature Secondary Structure
MQPFYITTPIYYVNAKPHLGHAYTTIVADALRRFHTLLGKKTFFLTGTDEHGDKIVQAAE
CCCEEEECCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHH
KNGCTPQEYVDSISATFRDLWPSMGITYDRFVRTTDESHKKCVQAVLQKVYDSGDIYFGE
HCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEC
YGGHYCYGCERFYTEKELENGLCPQHQVKPEYIAEKNYFFRMSKYQDWLRQYILDNPDFI
CCCEEEECHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCEEEHHHHHHHHHHHHCCCCCCC
RPERYRNEVLSLLDSGALEDLCISRPKSRLSWGVELPFDTDYVCYVWFDALLNYVSALNW
CCHHHHHHHHHHHHCCCHHHHHHCCCHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHCCC
PDGEEFTTFWPGVQHLVAKDILKPHAVFWPTMLKAAGFEPYNNLNVHGYWLVRDTKMSKS
CCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCHHHHH
LGNVIEPLEMAGRYGLSAFRYFLLREMHFGSDASFSEEALVARLNADLANDLGNLFSRVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SMTGKYFDGVVPSPAAYTADDEDIINLAKDACRNYVRLFERVRFSNALESLWELVRALNK
HHCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YVDSSAPWTLYKTGDTARLGTVLYVLLECMRKVAVNLWPVMPEASEAMLGQLHVCFDSAG
HHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHEECCCC
TRLQDEAEAWGTLQAGGKVEVSSGLFPRVEVAKEKDRPAEQPVPKAQPVAVPAVEYAEFE
CCCHHHHHHCCEECCCCEEEECCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHH
DFQKVELRVGTVLTVENHPKADKLYRIEVDLGEEQPRQIIAGLKEFFAPDALTGRQVVVV
HHHHHEEEEEEEEEECCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCEEEEE
ANLKPRKLRGLESQGMILALRTEDGMELLTASNPVPAGTRAS
ECCCCHHHCCCCCCCEEEEEECCCCCEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1993699; 10673435 [H]