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The map label for this gene is pfp [H]

Identifier: 78356632

GI number: 78356632

Start: 1618209

End: 1619561

Strand: Reverse

Name: pfp [H]

Synonym: Dde_1589

Alternate gene names: 78356632

Gene position: 1619561-1618209 (Counterclockwise)

Preceding gene: 78356633

Following gene: 78356631

Centisome position: 43.42

GC content: 55.88

Gene sequence:

>1353_bases
ATGACTGCCACCCGTTTTGAAAGCGGTTCCATAGTTCTGGACACCACCATCCCCAATCTGGGACCGGCCAAAATTCCGTC
GCCTCTGTCATACTGCCGCTTTGCCAACGAAGAGCAGGGACTCAGTCTGTTTCTTGATCACGATCAGGTTGAAGAACTGG
GCGATGAAGTAGTTCCGCTCACATTCGAGCCTGCCGGCCCCCGCCGTCATGTCTACTTTGACACTTCCAAAACCAAATGC
GCCATTGTCACCTGCGGCGGGCTGTGCCCCGGCATCAATGATGTCATCCGCGCCATAGTGATGGAAGCGTACCACAACTA
CCGCGTTCCCGCAGTGCTGGGCATCAAGTACGGGCTTGAAGGGTTCATTCCCCGCTACGGCCATGACGTGGTGGAACTGA
CGCCCGCCCTTGTGGGCGACATACACCAGTTCGGCGGCACCATTCTGGGTTCTTCGCGCGGGCCGCAGTCGCCGGAAGAA
ATAGTGGACGCTCTGGAGCGGATGAACGTCAATATTCTTTTTATGATTGGGGGCGACGGCACCATGAAGGCCGTCAGATC
CATAACCGGCGAGATCAATCAGCGCAGGCTGAAAATCAGCGTGATAGGCATTCCCAAAACCATCGACAATGATATCAATT
TCATCAGCCAGTCATTCGGGTTTGAAACCGCTGTGGACAAAGCCACCGAGGCTATTCAGTGCGCACACACCGAAGCTGTG
GGCGCCATGAACGGCATAGGACTGGTCAAACTCATGGGGCGCGAATCGGGCTTCATTGCCGCGCAGGCGACATTGTCGCT
CAAAGAAGTGAATTTTGTTCTGGTACCGGAGACTCCTTTCACCCTGCACGGTGAAGACGGTCTGCTCAATGCACTGGCCC
AGCGGCTGCGCGAACGCAGCCATGCCGTCATCGTGGTGGCAGAAGGTGCCGGCCAGCATCTGCTTTCGGGTTCGCATGAC
ACCGACGCCAGCGGCAACCCTATTCTGGGCGACATTGCAACGCTGCTGCGCACGGAAATCAAATCACACTTTGACAAACT
GGGCATACCGCACACGCTTAAATACATAGACCCCAGTTATATCATCAGATCAGTCCCCGCCAATGCCAACGACAGGGTAT
ACTGCGGTTTTCTGGGTCAAAACGCCGTACACGCCGGCATGGCCGGTAAAACAGGCATGGTCGTGGCCAAACTGATGGAC
AGGTATATTCATCTTCCACTGGACCTTGTGACCAGCAAGCGGCGCAAACTCAATATCATCTCCGACTTCTGGCGTGCGGT
GCTCGAATCAACAGGTCAGAGCCATCTGGCCGGTATGGTTCCGCCCGAAGCCAGAAGCTGCCCCAGAGCGTAA

Upstream 100 bases:

>100_bases
GGGCAAATCCCGCTATCGTCATGTGAGGCATCTGGAAAAGCAAACAACCATGTGATACTTTTGCCTTTGTTCATCAGCCA
ACCTGTATTCAGGAGGAAGC

Downstream 100 bases:

>100_bases
CATGCTGCAACAGATAAAGGCGTCTGCCGGTTCCGGCAAGACGTACCGGCTTACCGGTGAATTTCTTGACCGGCTGCGCG
CCGCGTCCGAAGAGTCTTCC

Product: diphosphate--fructose-6-phosphate 1-phosphotransferase

Products: NA

Alternate protein names: 6-phosphofructokinase, pyrophosphate dependent; PPi-dependent phosphofructokinase; PPi-PFK; Pyrophosphate-dependent 6-phosphofructose-1-kinase [H]

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MTATRFESGSIVLDTTIPNLGPAKIPSPLSYCRFANEEQGLSLFLDHDQVEELGDEVVPLTFEPAGPRRHVYFDTSKTKC
AIVTCGGLCPGINDVIRAIVMEAYHNYRVPAVLGIKYGLEGFIPRYGHDVVELTPALVGDIHQFGGTILGSSRGPQSPEE
IVDALERMNVNILFMIGGDGTMKAVRSITGEINQRRLKISVIGIPKTIDNDINFISQSFGFETAVDKATEAIQCAHTEAV
GAMNGIGLVKLMGRESGFIAAQATLSLKEVNFVLVPETPFTLHGEDGLLNALAQRLRERSHAVIVVAEGAGQHLLSGSHD
TDASGNPILGDIATLLRTEIKSHFDKLGIPHTLKYIDPSYIIRSVPANANDRVYCGFLGQNAVHAGMAGKTGMVVAKLMD
RYIHLPLDLVTSKRRKLNIISDFWRAVLESTGQSHLAGMVPPEARSCPRA

Sequences:

>Translated_450_residues
MTATRFESGSIVLDTTIPNLGPAKIPSPLSYCRFANEEQGLSLFLDHDQVEELGDEVVPLTFEPAGPRRHVYFDTSKTKC
AIVTCGGLCPGINDVIRAIVMEAYHNYRVPAVLGIKYGLEGFIPRYGHDVVELTPALVGDIHQFGGTILGSSRGPQSPEE
IVDALERMNVNILFMIGGDGTMKAVRSITGEINQRRLKISVIGIPKTIDNDINFISQSFGFETAVDKATEAIQCAHTEAV
GAMNGIGLVKLMGRESGFIAAQATLSLKEVNFVLVPETPFTLHGEDGLLNALAQRLRERSHAVIVVAEGAGQHLLSGSHD
TDASGNPILGDIATLLRTEIKSHFDKLGIPHTLKYIDPSYIIRSVPANANDRVYCGFLGQNAVHAGMAGKTGMVVAKLMD
RYIHLPLDLVTSKRRKLNIISDFWRAVLESTGQSHLAGMVPPEARSCPRA
>Mature_449_residues
TATRFESGSIVLDTTIPNLGPAKIPSPLSYCRFANEEQGLSLFLDHDQVEELGDEVVPLTFEPAGPRRHVYFDTSKTKCA
IVTCGGLCPGINDVIRAIVMEAYHNYRVPAVLGIKYGLEGFIPRYGHDVVELTPALVGDIHQFGGTILGSSRGPQSPEEI
VDALERMNVNILFMIGGDGTMKAVRSITGEINQRRLKISVIGIPKTIDNDINFISQSFGFETAVDKATEAIQCAHTEAVG
AMNGIGLVKLMGRESGFIAAQATLSLKEVNFVLVPETPFTLHGEDGLLNALAQRLRERSHAVIVVAEGAGQHLLSGSHDT
DASGNPILGDIATLLRTEIKSHFDKLGIPHTLKYIDPSYIIRSVPANANDRVYCGFLGQNAVHAGMAGKTGMVVAKLMDR
YIHLPLDLVTSKRRKLNIISDFWRAVLESTGQSHLAGMVPPEARSCPRA

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI266453619, Length=354, Percent_Identity=25.1412429378531, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI266453768, Length=354, Percent_Identity=25.1412429378531, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI266453748, Length=354, Percent_Identity=25.1412429378531, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI4505749, Length=354, Percent_Identity=25.1412429378531, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI11321601, Length=252, Percent_Identity=26.984126984127, Blast_Score=73, Evalue=7e-13,
Organism=Homo sapiens, GI48762920, Length=331, Percent_Identity=25.9818731117825, Blast_Score=70, Evalue=6e-12,
Organism=Escherichia coli, GI1790350, Length=332, Percent_Identity=27.710843373494, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI25147584, Length=332, Percent_Identity=28.6144578313253, Blast_Score=79, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17558788, Length=252, Percent_Identity=29.3650793650794, Blast_Score=78, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6323861, Length=334, Percent_Identity=25.1497005988024, Blast_Score=70, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6321679, Length=333, Percent_Identity=23.4234234234234, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI28573326, Length=334, Percent_Identity=26.3473053892216, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI17647809, Length=333, Percent_Identity=26.7267267267267, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24652337, Length=334, Percent_Identity=26.9461077844311, Blast_Score=83, Evalue=4e-16,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012829
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.90 [H]

Molecular weight: Translated: 48717; Mature: 48585

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATRFESGSIVLDTTIPNLGPAKIPSPLSYCRFANEEQGLSLFLDHDQVEELGDEVVPL
CCCCEECCCCEEEEECCCCCCCCCCCCHHHHHHCCCCCCCCEEEECCHHHHHCCCCEEEE
TFEPAGPRRHVYFDTSKTKCAIVTCGGLCPGINDVIRAIVMEAYHNYRVPAVLGIKYGLE
EECCCCCCEEEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEHHCHH
GFIPRYGHDVVELTPALVGDIHQFGGTILGSSRGPQSPEEIVDALERMNVNILFMIGGDG
HCCCCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHCCCEEEEEECCCH
TMKAVRSITGEINQRRLKISVIGIPKTIDNDINFISQSFGFETAVDKATEAIQCAHTEAV
HHHHHHHHHCCCCCEEEEEEEEECCCHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
GAMNGIGLVKLMGRESGFIAAQATLSLKEVNFVLVPETPFTLHGEDGLLNALAQRLRERS
HCCCCCCEEEEECCCCCEEEEEEEEEEEEEEEEEECCCCEEEECCCHHHHHHHHHHHHCC
HAVIVVAEGAGQHLLSGSHDTDASGNPILGDIATLLRTEIKSHFDKLGIPHTLKYIDPSY
CEEEEEECCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHH
IIRSVPANANDRVYCGFLGQNAVHAGMAGKTGMVVAKLMDRYIHLPLDLVTSKRRKLNII
HHEECCCCCCCEEEEEECCCCCHHCCCCCCHHHHHHHHHHHHHCCCHHHHHCCHHHHHHH
SDFWRAVLESTGQSHLAGMVPPEARSCPRA
HHHHHHHHHHCCCHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TATRFESGSIVLDTTIPNLGPAKIPSPLSYCRFANEEQGLSLFLDHDQVEELGDEVVPL
CCCEECCCCEEEEECCCCCCCCCCCCHHHHHHCCCCCCCCEEEECCHHHHHCCCCEEEE
TFEPAGPRRHVYFDTSKTKCAIVTCGGLCPGINDVIRAIVMEAYHNYRVPAVLGIKYGLE
EECCCCCCEEEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEHHCHH
GFIPRYGHDVVELTPALVGDIHQFGGTILGSSRGPQSPEEIVDALERMNVNILFMIGGDG
HCCCCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHCCCEEEEEECCCH
TMKAVRSITGEINQRRLKISVIGIPKTIDNDINFISQSFGFETAVDKATEAIQCAHTEAV
HHHHHHHHHCCCCCEEEEEEEEECCCHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
GAMNGIGLVKLMGRESGFIAAQATLSLKEVNFVLVPETPFTLHGEDGLLNALAQRLRERS
HCCCCCCEEEEECCCCCEEEEEEEEEEEEEEEEEECCCCEEEECCCHHHHHHHHHHHHCC
HAVIVVAEGAGQHLLSGSHDTDASGNPILGDIATLLRTEIKSHFDKLGIPHTLKYIDPSY
CEEEEEECCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHH
IIRSVPANANDRVYCGFLGQNAVHAGMAGKTGMVVAKLMDRYIHLPLDLVTSKRRKLNII
HHEECCCCCCCEEEEEECCCCCHHCCCCCCHHHHHHHHHHHHHCCCHHHHHCCHHHHHHH
SDFWRAVLESTGQSHLAGMVPPEARSCPRA
HHHHHHHHHHCCCHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10913106 [H]