Click here to switch to the map view.

The map label for this gene is nth [C]

Identifier: 78356480

GI number: 78356480

Start: 1459539

End: 1460204

Strand: Direct

Name: nth [C]

Synonym: Dde_1435

Alternate gene names: 78356480

Gene position: 1459539-1460204 (Clockwise)

Preceding gene: 78356479

Following gene: 78356481

Centisome position: 39.13

GC content: 59.76

Gene sequence:

>666_bases
ATGAACCGAGAGACACTCCTGATGCAGATGTTCGGCGCCATGCTGGCCCGTCTGGGCCCCAGCGGCTGGTGGCCTGCCGG
TTCCCCTTTTGAGGTGGCGGCGGGAGCGGTGCTGACCCAGAATGCCAGCTGGACCAATGCCGGGCAGGCTCTGCAGCGGA
TGCGGTCTGCGGACATGCTTTCCGAATCGGGCGTGGCCGCAGCGGCACCTGACACGCTGGAAGAGCTGGTGCGGCCTGCG
GGTTTTTACCGGCAGAAGGCCGCCACCCTGCGGCGGCTTGTACAGTTTTTGTACGAAGAAGCGGACGGTGACATCCGCAA
CCTTGCGGGAACTTCCATGGACAGTCTGCGCGCGCGGCTGCTGGACATAAAAGGCATAGGGCCTGAAACGGCAGATTCCA
TTCTGCTGTACGCGCTTGATATGCCCAGCTTTGTGGTGGATGCGTATACGCGGCGTATCTGCGTGCGGCACGGCCTGTTG
CCCGAAGATGTGCAATATGCCGAAATGCGCGAATTTTTCATGGACGTTCTGCCTGCGGATGTATCAGTATACAATGAATA
TCATGCCCTGCTTGTACGTGTGGCCAAAGAATGGTGCCGCAAAACGCACCCGCGCTGCGACTCCTGCCCGTTACGGCAGT
TTCTTGAATACGAGGTGGTCTGCTGA

Upstream 100 bases:

>100_bases
GCACCGGCGCCTGCAAGGCGCGGAATGTATCTTGAAGCTGTCGCAAAGTACAGTCTGGCTCATTGCGCTTCGACCGGCAA
CTCTATAGGATGACAACGCT

Downstream 100 bases:

>100_bases
TGCGTGGATTTGTGGTGCGGGCTGTTCTGGTGGCCCTGCTGTGCTGCTGCACGGCTGTTACTGCCGTTCAGGCGCAGGAC
CTGACGCGTTCCATCAGGGA

Product: DNA-3-methyladenine glycosylase III

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 221; Mature: 221

Protein sequence:

>221_residues
MNRETLLMQMFGAMLARLGPSGWWPAGSPFEVAAGAVLTQNASWTNAGQALQRMRSADMLSESGVAAAAPDTLEELVRPA
GFYRQKAATLRRLVQFLYEEADGDIRNLAGTSMDSLRARLLDIKGIGPETADSILLYALDMPSFVVDAYTRRICVRHGLL
PEDVQYAEMREFFMDVLPADVSVYNEYHALLVRVAKEWCRKTHPRCDSCPLRQFLEYEVVC

Sequences:

>Translated_221_residues
MNRETLLMQMFGAMLARLGPSGWWPAGSPFEVAAGAVLTQNASWTNAGQALQRMRSADMLSESGVAAAAPDTLEELVRPA
GFYRQKAATLRRLVQFLYEEADGDIRNLAGTSMDSLRARLLDIKGIGPETADSILLYALDMPSFVVDAYTRRICVRHGLL
PEDVQYAEMREFFMDVLPADVSVYNEYHALLVRVAKEWCRKTHPRCDSCPLRQFLEYEVVC
>Mature_221_residues
MNRETLLMQMFGAMLARLGPSGWWPAGSPFEVAAGAVLTQNASWTNAGQALQRMRSADMLSESGVAAAAPDTLEELVRPA
GFYRQKAATLRRLVQFLYEEADGDIRNLAGTSMDSLRARLLDIKGIGPETADSILLYALDMPSFVVDAYTRRICVRHGLL
PEDVQYAEMREFFMDVLPADVSVYNEYHALLVRVAKEWCRKTHPRCDSCPLRQFLEYEVVC

Specific function: Has Both An Apurinic And/Or Apyrimidinic Endonuclease Activity And A DNA N-Glycosylase Activity. Incises Damaged DNA At Cytosines, Thymines And Guanines. Acts On A Damaged Strand, 5' From The Damaged Site. Required For The Repair Of Both Oxidative DNA Da

COG id: COG2231

COG function: function code L; Uncharacterized protein related to Endonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Escherichia coli, GI1787920, Length=204, Percent_Identity=25, Blast_Score=68, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF00730 HhH-GPD [H]

EC number: 4.2.99.18

Molecular weight: Translated: 24772; Mature: 24772

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRETLLMQMFGAMLARLGPSGWWPAGSPFEVAAGAVLTQNASWTNAGQALQRMRSADML
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
SESGVAAAAPDTLEELVRPAGFYRQKAATLRRLVQFLYEEADGDIRNLAGTSMDSLRARL
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHH
LDIKGIGPETADSILLYALDMPSFVVDAYTRRICVRHGLLPEDVQYAEMREFFMDVLPAD
HHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCHH
VSVYNEYHALLVRVAKEWCRKTHPRCDSCPLRQFLEYEVVC
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MNRETLLMQMFGAMLARLGPSGWWPAGSPFEVAAGAVLTQNASWTNAGQALQRMRSADML
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
SESGVAAAAPDTLEELVRPAGFYRQKAATLRRLVQFLYEEADGDIRNLAGTSMDSLRARL
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHH
LDIKGIGPETADSILLYALDMPSFVVDAYTRRICVRHGLLPEDVQYAEMREFFMDVLPAD
HHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCHH
VSVYNEYHALLVRVAKEWCRKTHPRCDSCPLRQFLEYEVVC
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: 4Fe-4S Cluster. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Carbon-Oxygen Lyase [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]