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The map label for this gene is fhlA [H]

Identifier: 78356441

GI number: 78356441

Start: 1412346

End: 1412804

Strand: Reverse

Name: fhlA [H]

Synonym: Dde_1396

Alternate gene names: 78356441

Gene position: 1412804-1412346 (Counterclockwise)

Preceding gene: 78356442

Following gene: 78356426

Centisome position: 37.87

GC content: 62.96

Gene sequence:

>459_bases
ATGTGGCAGGATCACTTTATAGCCAAAGCCAGCAGACGGCTGGGCATACCGGAACCGCGCCTGCCCTACCGTCATATCCG
CAGGCTGGCGGCGTATCACTGGCCCGGTAACGTGCGCGAACTGCAGAACATCATGGAGCGGGCAGTCATCATGGCCGACG
GTGGCGTAATATCTGCCGAGGCACTGCCTGAAGCACTGCCCCGGCGCCGGGAAGAGGCGCAACCGGGCGCACTTACGCGC
AATGAATACGGCTGGCAGACCGAAGCACCTGACCGGCGGGTGACAGTGATCCGTGAAGAACAATGGGCGCACATGCAGCG
CGAAAACATCCGTGCCGCGCTGGAAGCCTGTAACGGACGCATACACGGGCCGGACGGCGCCGCCCACCTGCTGGGGGTCC
GCCCCACGACCCTCCAGTCGCGTATCAAAAAGTTCGGCATAGGCAGACAGGACGTATGA

Upstream 100 bases:

>100_bases
CGTCGTCGGAGGCATTCCAGTAACGGTTGGATGTCTCCTGCGGCTTTCGAAAATTTCAACCAGGAATACAAAAAAGTAGC
TTAACTGGTACTCCACAAAA

Downstream 100 bases:

>100_bases
CGCACCCCGCGGCGGGGCATCTGCCGCTTCACCATTCTCCCGCCCTGAAACAACAAAGCCCGTACAGACGGGCTTTGTTG
TTTCTAAGCTTCCAGATCAC

Product: Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 152; Mature: 152

Protein sequence:

>152_residues
MWQDHFIAKASRRLGIPEPRLPYRHIRRLAAYHWPGNVRELQNIMERAVIMADGGVISAEALPEALPRRREEAQPGALTR
NEYGWQTEAPDRRVTVIREEQWAHMQRENIRAALEACNGRIHGPDGAAHLLGVRPTTLQSRIKKFGIGRQDV

Sequences:

>Translated_152_residues
MWQDHFIAKASRRLGIPEPRLPYRHIRRLAAYHWPGNVRELQNIMERAVIMADGGVISAEALPEALPRRREEAQPGALTR
NEYGWQTEAPDRRVTVIREEQWAHMQRENIRAALEACNGRIHGPDGAAHLLGVRPTTLQSRIKKFGIGRQDV
>Mature_152_residues
MWQDHFIAKASRRLGIPEPRLPYRHIRRLAAYHWPGNVRELQNIMERAVIMADGGVISAEALPEALPRRREEAQPGALTR
NEYGWQTEAPDRRVTVIREEQWAHMQRENIRAALEACNGRIHGPDGAAHLLGVRPTTLQSRIKKFGIGRQDV

Specific function: Required for induction of expression of the formate dehydrogenase H and hydrogenase-3 structural genes [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1789087, Length=145, Percent_Identity=35.8620689655172, Blast_Score=77, Evalue=7e-16,
Organism=Escherichia coli, GI87082117, Length=148, Percent_Identity=32.4324324324324, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 17397; Mature: 17397

Theoretical pI: Translated: 10.69; Mature: 10.69

Prosite motif: PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWQDHFIAKASRRLGIPEPRLPYRHIRRLAAYHWPGNVRELQNIMERAVIMADGGVISAE
CCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEECHH
ALPEALPRRREEAQPGALTRNEYGWQTEAPDRRVTVIREEQWAHMQRENIRAALEACNGR
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCC
IHGPDGAAHLLGVRPTTLQSRIKKFGIGRQDV
CCCCCCCHHEECCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MWQDHFIAKASRRLGIPEPRLPYRHIRRLAAYHWPGNVRELQNIMERAVIMADGGVISAE
CCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEECHH
ALPEALPRRREEAQPGALTRNEYGWQTEAPDRRVTVIREEQWAHMQRENIRAALEACNGR
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCC
IHGPDGAAHLLGVRPTTLQSRIKKFGIGRQDV
CCCCCCCHHEECCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2280686; 2118503; 9278503 [H]