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The map label for this gene is trxB [C]

Identifier: 78356250

GI number: 78356250

Start: 1230259

End: 1231176

Strand: Direct

Name: trxB [C]

Synonym: Dde_1203

Alternate gene names: 78356250

Gene position: 1230259-1231176 (Clockwise)

Preceding gene: 78356249

Following gene: 78356251

Centisome position: 32.98

GC content: 58.5

Gene sequence:

>918_bases
ATGAAAGAATCCAGACAGGTCGATATAGTCATTCTGGGGGGCGGACCTGCGGGCATGACCGCCGCCATTTATGCTGCCAG
AGCAAGCCTGAATGTGGCCCTGCTTGAAGAAAGCGTCACGGGCGGGCTGGTCAATTCCACATATACTGTCGAAAATTTTC
CGTCGTATCCGGCCATACACGGTATGGAACTGATGCAGAAAATGCGTGACCACGTGGACAGCCTGGGCGTGACGGTGGAG
GAAGTGTGCGAAATAGAATCTGTTTCGCTTGCCGGTCAGGTGAAAACCGTGGAAACGGATGAGGCGGTGTTTCAGGCACG
GGCTGTCATTCTGGCCACCGGACGTAAGCCCGTGCCGCTTGATGTGCCCACCGGCTGTGAACAGGTGCACCACTGCGCCA
TATGCGACGGCGCCGCTTACAAAGGCCGCCGCGTGCTGGTGGTGGGCGGCGGCAACAGTGCTTTTGACGAAAGCCTGTAC
ATGCTGGGCATAGGTGTGGAGCACATAACGCTTGTGGAAAAAATGGACCGTTTTTTTGCCGCGCAGTCCACGCAGGACAC
CCTGCTGGAAACAGGCAGGGTGGATGCCCGTACGCAGACCGTGGTGCATGACCTGATAGTGGAGAATGACTCGTTGAAGG
CCGCCGTGCTGGAGCACACCGGAAGCGGCGTGCGCGAGACGGTACCCGTGGATGGCGTGTTCGTGTTTCTGGGACAGAGC
CCCAATAACGATCTGTTCAGAGACGTGGTGACCCTGAGCGCGGGCGGCTATGTGGTGGTGGACGAACATATGGCCACCAG
CGTGTCCGGCGTGTTCAGCGCGGGTGATATAAACGAAAAGCACTACCGGCAGATAACCACAGCCATGGCGGACGGCACCG
TTGCGGCGCTGGCGGCCGAACGCTACATCAGATCGTAA

Upstream 100 bases:

>100_bases
GAAATGAAGGCGTTTTTCGCCGCGGCGTAGCCGTATTTGCATATGCAACCGCCCGCATTGTCCGGGGAGGGCATGCGGGC
GCTCAAAAGAGATACGCATC

Downstream 100 bases:

>100_bases
CGGAGCCTGTTCATGTTCATATCAAACAAATCTCTGCGTAACTGGCTGCTGGTGGCGGGCATTGCCGGTGCCGGCGCTTT
TGCGCTGCATAATCCCGAAA

Product: thioredoxin reductase

Products: thioredoxin disulfide; NADPH; H+

Alternate protein names: ORF A [H]

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MKESRQVDIVILGGGPAGMTAAIYAARASLNVALLEESVTGGLVNSTYTVENFPSYPAIHGMELMQKMRDHVDSLGVTVE
EVCEIESVSLAGQVKTVETDEAVFQARAVILATGRKPVPLDVPTGCEQVHHCAICDGAAYKGRRVLVVGGGNSAFDESLY
MLGIGVEHITLVEKMDRFFAAQSTQDTLLETGRVDARTQTVVHDLIVENDSLKAAVLEHTGSGVRETVPVDGVFVFLGQS
PNNDLFRDVVTLSAGGYVVVDEHMATSVSGVFSAGDINEKHYRQITTAMADGTVAALAAERYIRS

Sequences:

>Translated_305_residues
MKESRQVDIVILGGGPAGMTAAIYAARASLNVALLEESVTGGLVNSTYTVENFPSYPAIHGMELMQKMRDHVDSLGVTVE
EVCEIESVSLAGQVKTVETDEAVFQARAVILATGRKPVPLDVPTGCEQVHHCAICDGAAYKGRRVLVVGGGNSAFDESLY
MLGIGVEHITLVEKMDRFFAAQSTQDTLLETGRVDARTQTVVHDLIVENDSLKAAVLEHTGSGVRETVPVDGVFVFLGQS
PNNDLFRDVVTLSAGGYVVVDEHMATSVSGVFSAGDINEKHYRQITTAMADGTVAALAAERYIRS
>Mature_305_residues
MKESRQVDIVILGGGPAGMTAAIYAARASLNVALLEESVTGGLVNSTYTVENFPSYPAIHGMELMQKMRDHVDSLGVTVE
EVCEIESVSLAGQVKTVETDEAVFQARAVILATGRKPVPLDVPTGCEQVHHCAICDGAAYKGRRVLVVGGGNSAFDESLY
MLGIGVEHITLVEKMDRFFAAQSTQDTLLETGRVDARTQTVVHDLIVENDSLKAAVLEHTGSGVRETVPVDGVFVFLGQS
PNNDLFRDVVTLSAGGYVVVDEHMATSVSGVFSAGDINEKHYRQITTAMADGTVAALAAERYIRS

Specific function: Unknown

COG id: COG0492

COG function: function code O; Thioredoxin reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787114, Length=317, Percent_Identity=33.4384858044164, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI87081763, Length=307, Percent_Identity=35.1791530944625, Blast_Score=140, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6321898, Length=311, Percent_Identity=32.475884244373, Blast_Score=142, Evalue=7e-35,
Organism=Saccharomyces cerevisiae, GI6320560, Length=313, Percent_Identity=31.3099041533546, Blast_Score=138, Evalue=1e-33,

Paralogues:

None

Copy number: 808 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR008255
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.8.1.9

Molecular weight: Translated: 32545; Mature: 32545

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS00573 PYRIDINE_REDOX_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKESRQVDIVILGGGPAGMTAAIYAARASLNVALLEESVTGGLVNSTYTVENFPSYPAIH
CCCCCEEEEEEECCCCCCHHHHHHHHHHCCEEEEEEHHHCCCCCCCEEEECCCCCCCCHH
GMELMQKMRDHVDSLGVTVEEVCEIESVSLAGQVKTVETDEAVFQARAVILATGRKPVPL
HHHHHHHHHHHHHHHCCCHHHHHCHHHEEECCEEEEEECHHHHHHHEEEEEECCCCCCCC
DVPTGCEQVHHCAICDGAAYKGRRVLVVGGGNSAFDESLYMLGIGVEHITLVEKMDRFFA
CCCCCHHHHHEEEEECCCCCCCCEEEEEECCCCCCCCCEEEEEECHHHHHHHHHHHHHHH
AQSTQDTLLETGRVDARTQTVVHDLIVENDSLKAAVLEHTGSGVRETVPVDGVFVFLGQS
CCCCHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCEEEEEEECC
PNNDLFRDVVTLSAGGYVVVDEHMATSVSGVFSAGDINEKHYRQITTAMADGTVAALAAE
CCCHHHHHHHHCCCCCEEEECCHHHHHHHHCEECCCCCHHHHHHHHHHHCCCHHHHHHHH
RYIRS
HHHCC
>Mature Secondary Structure
MKESRQVDIVILGGGPAGMTAAIYAARASLNVALLEESVTGGLVNSTYTVENFPSYPAIH
CCCCCEEEEEEECCCCCCHHHHHHHHHHCCEEEEEEHHHCCCCCCCEEEECCCCCCCCHH
GMELMQKMRDHVDSLGVTVEEVCEIESVSLAGQVKTVETDEAVFQARAVILATGRKPVPL
HHHHHHHHHHHHHHHCCCHHHHHCHHHEEECCEEEEEECHHHHHHHEEEEEECCCCCCCC
DVPTGCEQVHHCAICDGAAYKGRRVLVVGGGNSAFDESLYMLGIGVEHITLVEKMDRFFA
CCCCCHHHHHEEEEECCCCCCCCEEEEEECCCCCCCCCEEEEEECHHHHHHHHHHHHHHH
AQSTQDTLLETGRVDARTQTVVHDLIVENDSLKAAVLEHTGSGVRETVPVDGVFVFLGQS
CCCCHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCEEEEEEECC
PNNDLFRDVVTLSAGGYVVVDEHMATSVSGVFSAGDINEKHYRQITTAMADGTVAALAAE
CCCHHHHHHHHCCCCCEEEECCHHHHHHHHCEECCCCCHHHHHHHHHHHCCCHHHHHHHH
RYIRS
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: NA

Kcat value (1/min): 1365 [C]

Specific activity: NA

Km value (mM): 0.008 {NADPH}} 0.125 {thioredoxin} 0.002 {thioredoxin}} 0.0017 {thioredoxin}} [C]

Substrates: thioredoxin; NADP+

Specific reaction: thioredoxin + NADP+ = thioredoxin disulfide + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: Ca2+; N-Ethyl maleimide; NADP+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1637309 [H]