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The map label for this gene is rnjA [H]

Identifier: 78356184

GI number: 78356184

Start: 1166021

End: 1167685

Strand: Direct

Name: rnjA [H]

Synonym: Dde_1137

Alternate gene names: 78356184

Gene position: 1166021-1167685 (Clockwise)

Preceding gene: 78356183

Following gene: 78356185

Centisome position: 31.26

GC content: 57.12

Gene sequence:

>1665_bases
ATGCCGGAACACCAAGACCCTTTCTTGACGCTGACGCCGCTGGGCGGCTGCGGTGAAATAGGACTGAACTGCACACTGTG
GTCAACCCCCGCGGGCAGTGTGCTTGTGGACTGCGGGCTGATGTTTCCGGACGACTACCATCTGGGCGTGGATGTGGTCA
TTCCCCGGCTTGAGTACATACTGGCGCAGAAAGAGCGCATGCTCGGCATTGTACTGACGCACGGGCATGAAGATCATATC
GGCGCGCTGCCCTGGCTGCTTCCGTACCTTCAGGTGCCCATTTACGGGTCGCGTTTCACGCTGGCGCTGGTGGAATCCAA
ACTGCGCGAGCACAACCTGCTGGACAGGGTGCGCCTGGTGACGGTGGGACCGGAAAAGCCGTGTCCCATCGGCGACATGG
TTTTTCATTTCTTTCCCGTGTGTCATTCCATTATCGAAGGATACGGGCTGGGGGTCGAAACTCCTGCGGGACGGGTGGTG
CATACCGGCGACTTCAAGATAGACCCCGACCCCATTGACGGGCATTTCACCGACCTTGAGGCCTTTCGCGGTTTTGCTCA
GGAAGACGGAGTGGACCTGCTCCTTTCGGATTCCACCAATATCGAGCGTGAAGGCTTTTCCGTTTCCGAGCGTGAAATAC
AGACCACGTTCACGCGGATTTTTTCGCAGGCGAAAGGTCGTGTGATTATTACTCTTTTTTCCAGCCATATTCAGAGAATA
CAACAGGTTTTCGACATCGCCGCCCGTTTCGGGCGCAAGGTTGCCGTTTCGGGCCGCAGCCTGATGCATAACGTGGATCT
GGCGCGAGAGCTCGGGTACCTGCGCATACTGGGCGGCACGTATGTGGATGCCGCCGACATAGGCGGCATGGCAGACGAAA
ATGTAGTGCTGCTGGTCACAGGTTCGCAGGGCGAGCCGTTTTCCGCCCTTTCGCGCATTGCCTCCGGCGAGCACAAGCTG
CTGGACATCCATCCCGGCGATACCGTCATCATGTCATCGCGGTTTATTCCCGGCAATACCCGGGCCATAACGCGGCTTAT
AAACAATCTGTACCGGCTGGGTGCAGAGGTGTACTACGACAAGGTGCAGGCCATTCATGCTTCGGGGCATGCGCATCGCG
AAGAGTTGCGTCTGATGCTGCAGACCGTGCGTCCGCGCCATTTTGTGCCGGTGCACGGAGAATACCGGCATCTGGTCAAG
CACAGCCGTCTGGCCGTGGAGTGCGGTGTACCGCCTGAGTGTTCGCTTATCCTTGAAGACGGCGATCCGCTGACTCTTAC
CCGCGACGGTGTGCGCCGCGAAGCGCATATCAATGTGGAAAGCGTGCTCGTGGACGGCAAGGGTGTGGGCGATGTGGGGC
AGTCTGTGCTGCGCGAACGTCATATCCTTGGCGGCGAGGGTATGGTTGTGGTAGTCATGGTTGTGGATGAAAATATCTGG
GAGATTCTGGTAGGCCCCGACATTGTCTCCAAGGGGTTTGTATTTGAGCAGCATTACAATCATGTGCTGGAAGATGCCAA
ATGCATTATTCTGGACCTGTTCGAAAATATTCCTCCCGGCGATGTGACATACCTGCAGGACAGGATACGCAGTACCCTGA
GGCGTTTTTTCCGCAAGGTACTTGAACGCGATCCTGTGGTGGTGCCCATGGTCACGGTGGTCTGA

Upstream 100 bases:

>100_bases
TGCCCCCTGTCGACCCTGCATCCTACTCCAAGGATGAGAAGAATATTTTCAGTGATACGCTGCGCGATTCCATGGAAATC
GCCTATCAGGAGATGCGTGC

Downstream 100 bases:

>100_bases
CCGGCAGCAGACGCGGCGGAATGCATGGGTCCGCCGCCGTGTCATGTGTTTTTCGCAATGCAGCGACCTGTGAAGGAGAA
AACCTGATGCGCGTGCTGAG

Product: metallo-beta-lactamase family protein

Products: NA

Alternate protein names: RNase J1 [H]

Number of amino acids: Translated: 554; Mature: 553

Protein sequence:

>554_residues
MPEHQDPFLTLTPLGGCGEIGLNCTLWSTPAGSVLVDCGLMFPDDYHLGVDVVIPRLEYILAQKERMLGIVLTHGHEDHI
GALPWLLPYLQVPIYGSRFTLALVESKLREHNLLDRVRLVTVGPEKPCPIGDMVFHFFPVCHSIIEGYGLGVETPAGRVV
HTGDFKIDPDPIDGHFTDLEAFRGFAQEDGVDLLLSDSTNIEREGFSVSEREIQTTFTRIFSQAKGRVIITLFSSHIQRI
QQVFDIAARFGRKVAVSGRSLMHNVDLARELGYLRILGGTYVDAADIGGMADENVVLLVTGSQGEPFSALSRIASGEHKL
LDIHPGDTVIMSSRFIPGNTRAITRLINNLYRLGAEVYYDKVQAIHASGHAHREELRLMLQTVRPRHFVPVHGEYRHLVK
HSRLAVECGVPPECSLILEDGDPLTLTRDGVRREAHINVESVLVDGKGVGDVGQSVLRERHILGGEGMVVVVMVVDENIW
EILVGPDIVSKGFVFEQHYNHVLEDAKCIILDLFENIPPGDVTYLQDRIRSTLRRFFRKVLERDPVVVPMVTVV

Sequences:

>Translated_554_residues
MPEHQDPFLTLTPLGGCGEIGLNCTLWSTPAGSVLVDCGLMFPDDYHLGVDVVIPRLEYILAQKERMLGIVLTHGHEDHI
GALPWLLPYLQVPIYGSRFTLALVESKLREHNLLDRVRLVTVGPEKPCPIGDMVFHFFPVCHSIIEGYGLGVETPAGRVV
HTGDFKIDPDPIDGHFTDLEAFRGFAQEDGVDLLLSDSTNIEREGFSVSEREIQTTFTRIFSQAKGRVIITLFSSHIQRI
QQVFDIAARFGRKVAVSGRSLMHNVDLARELGYLRILGGTYVDAADIGGMADENVVLLVTGSQGEPFSALSRIASGEHKL
LDIHPGDTVIMSSRFIPGNTRAITRLINNLYRLGAEVYYDKVQAIHASGHAHREELRLMLQTVRPRHFVPVHGEYRHLVK
HSRLAVECGVPPECSLILEDGDPLTLTRDGVRREAHINVESVLVDGKGVGDVGQSVLRERHILGGEGMVVVVMVVDENIW
EILVGPDIVSKGFVFEQHYNHVLEDAKCIILDLFENIPPGDVTYLQDRIRSTLRRFFRKVLERDPVVVPMVTVV
>Mature_553_residues
PEHQDPFLTLTPLGGCGEIGLNCTLWSTPAGSVLVDCGLMFPDDYHLGVDVVIPRLEYILAQKERMLGIVLTHGHEDHIG
ALPWLLPYLQVPIYGSRFTLALVESKLREHNLLDRVRLVTVGPEKPCPIGDMVFHFFPVCHSIIEGYGLGVETPAGRVVH
TGDFKIDPDPIDGHFTDLEAFRGFAQEDGVDLLLSDSTNIEREGFSVSEREIQTTFTRIFSQAKGRVIITLFSSHIQRIQ
QVFDIAARFGRKVAVSGRSLMHNVDLARELGYLRILGGTYVDAADIGGMADENVVLLVTGSQGEPFSALSRIASGEHKLL
DIHPGDTVIMSSRFIPGNTRAITRLINNLYRLGAEVYYDKVQAIHASGHAHREELRLMLQTVRPRHFVPVHGEYRHLVKH
SRLAVECGVPPECSLILEDGDPLTLTRDGVRREAHINVESVLVDGKGVGDVGQSVLRERHILGGEGMVVVVMVVDENIWE
ILVGPDIVSKGFVFEQHYNHVLEDAKCIILDLFENIPPGDVTYLQDRIRSTLRRFFRKVLERDPVVVPMVTVV

Specific function: Cleaves the 5'-leader sequence of certain mRNAs. May play a role in maturation and stability of specific mRNAs. Is also required for the maturation of the 16S rRNA. Acts preferentially on 16S rRNA precursors after association of the 30S and 50S ribosomal

COG id: COG0595

COG function: function code R; Predicted hydrolase of the metallo-beta-lactamase superfamily

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. RNase J subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR011108
- InterPro:   IPR004613
- InterPro:   IPR001587 [H]

Pfam domain/function: PF00753 Lactamase_B; PF07521 RMMBL [H]

EC number: NA

Molecular weight: Translated: 61673; Mature: 61542

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS01292 UPF0036

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEHQDPFLTLTPLGGCGEIGLNCTLWSTPAGSVLVDCGLMFPDDYHLGVDVVIPRLEYI
CCCCCCCEEEEECCCCCCCCCCEEEEECCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHH
LAQKERMLGIVLTHGHEDHIGALPWLLPYLQVPIYGSRFTLALVESKLREHNLLDRVRLV
HHHHHHEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHEEEE
TVGPEKPCPIGDMVFHFFPVCHSIIEGYGLGVETPAGRVVHTGDFKIDPDPIDGHFTDLE
EECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCEECCCCCCCCCCCHH
AFRGFAQEDGVDLLLSDSTNIEREGFSVSEREIQTTFTRIFSQAKGRVIITLFSSHIQRI
HHCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHH
QQVFDIAARFGRKVAVSGRSLMHNVDLARELGYLRILGGTYVDAADIGGMADENVVLLVT
HHHHHHHHHHCCEEEECCHHHHHHHHHHHHCCEEEEECCCEECHHHCCCCCCCCEEEEEE
GSQGEPFSALSRIASGEHKLLDIHPGDTVIMSSRFIPGNTRAITRLINNLYRLGAEVYYD
CCCCCCHHHHHHHCCCCCEEEEECCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
KVQAIHASGHAHREELRLMLQTVRPRHFVPVHGEYRHLVKHSRLAVECGVPPECSLILED
HHHHHHCCCCCHHHHHHHHHHHCCCCCEEECCCHHHHHHHHCCEEEECCCCCCCEEEEEC
GDPLTLTRDGVRREAHINVESVLVDGKGVGDVGQSVLRERHILGGEGMVVVVMVVDENIW
CCCEEEECCCCCCEEECCHHHEEECCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCE
EILVGPDIVSKGFVFEQHYNHVLEDAKCIILDLFENIPPGDVTYLQDRIRSTLRRFFRKV
EEEECCCHHHCCEEEHHHHHHHHHCCCEEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
LERDPVVVPMVTVV
HHCCCEEEEEEEEC
>Mature Secondary Structure 
PEHQDPFLTLTPLGGCGEIGLNCTLWSTPAGSVLVDCGLMFPDDYHLGVDVVIPRLEYI
CCCCCCEEEEECCCCCCCCCCEEEEECCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHH
LAQKERMLGIVLTHGHEDHIGALPWLLPYLQVPIYGSRFTLALVESKLREHNLLDRVRLV
HHHHHHEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHEEEE
TVGPEKPCPIGDMVFHFFPVCHSIIEGYGLGVETPAGRVVHTGDFKIDPDPIDGHFTDLE
EECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCEECCCCCCCCCCCHH
AFRGFAQEDGVDLLLSDSTNIEREGFSVSEREIQTTFTRIFSQAKGRVIITLFSSHIQRI
HHCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHH
QQVFDIAARFGRKVAVSGRSLMHNVDLARELGYLRILGGTYVDAADIGGMADENVVLLVT
HHHHHHHHHHCCEEEECCHHHHHHHHHHHHCCEEEEECCCEECHHHCCCCCCCCEEEEEE
GSQGEPFSALSRIASGEHKLLDIHPGDTVIMSSRFIPGNTRAITRLINNLYRLGAEVYYD
CCCCCCHHHHHHHCCCCCEEEEECCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
KVQAIHASGHAHREELRLMLQTVRPRHFVPVHGEYRHLVKHSRLAVECGVPPECSLILED
HHHHHHCCCCCHHHHHHHHHHHCCCCCEEECCCHHHHHHHHCCEEEECCCCCCCEEEEEC
GDPLTLTRDGVRREAHINVESVLVDGKGVGDVGQSVLRERHILGGEGMVVVVMVVDENIW
CCCEEEECCCCCCEEECCHHHEEECCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCE
EILVGPDIVSKGFVFEQHYNHVLEDAKCIILDLFENIPPGDVTYLQDRIRSTLRRFFRKV
EEEECCCHHHCCEEEHHHHHHHHHCCCEEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
LERDPVVVPMVTVV
HHCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969500; 9384377 [H]