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The map label for this gene is mraW

Identifier: 78356081

GI number: 78356081

Start: 1060481

End: 1061458

Strand: Direct

Name: mraW

Synonym: Dde_1034

Alternate gene names: 78356081

Gene position: 1060481-1061458 (Clockwise)

Preceding gene: 78356080

Following gene: 78356082

Centisome position: 28.43

GC content: 61.35

Gene sequence:

>978_bases
GTGACGGCAGACAACGGGCATGACAACAACCGGCATCAGTACACAGCTCATGTTCCTGTGCTGCTGGACGAGGTGCTGCA
TTATCTTTCGCCGGTCAGGGGCGGGCGGTATCTGGACGGTACGCTCGGTCTTGGCGGACACAGCGAAGCGATAATGAACC
GCTGCGGCGGAGATGCCTGGCTGCTGGGGCTGGACCGTGACCGCGAGGCGCTTGCCGCCGCATCCGGAAGGCTTGCTCCT
TTCGGCGACCGCGTGACCACCCGCTATGCCTGTTACAGTCAGTTTGCCGCCATCATGGACGAAATTGGCTGGACCGGGCT
GGACGGGGCGCTGATAGACATCGGTGTTTCCTCTATGCAGATAGACACTCCTTCCCGCGGATTCAGCTTTTACGCTGACG
GCCCGCTGGACATGCGCATGGACCCTTCCGGTGATATGCCGTCTGCCGGTGTGCTGGTGAACACAGGCAGCGTGGAGCGT
CTTAAGGAAATCATAAGCACGTACGGCGAAGATCCCATGGCGGGCAGGATAGCCCGGGCCATCGTTGACGCGCGTGCCCG
CAATCCCATAGAAACCACGGCCCGTCTGGCCGAGGTGGTGGAAAGCGCCTATCCGGCAAAATGGCGGGCAAAATCCCGCA
ACCATCCGGCAACGCGCACGTTTCAGGCGCTGCGCATGGCCGTGAACGGAGAGCTGGAGGAACTGGAGACCTTTCTTGCC
GCCATCGTCGACAGGATGAATCCCGGCGGACGTATTGTGGTGATCACCTTTCATTCACTGGAAGACCGGCTGGTCAAAAA
CGCATTCAGAGATGAAGCCAAGGGCTGTCTGTGCCCCAGGCATATTCCCGTGTGCGTGTGCGGCAAAAAGCCGCGGGTCA
ATGTGCTGACCCGCAAACCGGTGACCGCGGGGCAGGCGGAGCTGCAGGCCAACAGCAGGGCCTCCAGCGCCAAGCTGCGT
GCCGCGGAGCGGATTTAA

Upstream 100 bases:

>100_bases
AAGCGGTGATAGGTCAGGACTTCGGGGACGTGACGGACGAACTGGCGGAAAGCGGTGTGGATTTTCCCATCTGAGGCAAC
AGTACAATGCGAGGCGACTT

Downstream 100 bases:

>100_bases
GGCGTTGTTATGCAGAAATACGGAAGCTGGATACTGGGCATGGTGCTTTCGCTCATCAGCGGACTGGTGCTGGGGTTGAC
ACTGGTGTGGCTGAATGTGG

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MTADNGHDNNRHQYTAHVPVLLDEVLHYLSPVRGGRYLDGTLGLGGHSEAIMNRCGGDAWLLGLDRDREALAAASGRLAP
FGDRVTTRYACYSQFAAIMDEIGWTGLDGALIDIGVSSMQIDTPSRGFSFYADGPLDMRMDPSGDMPSAGVLVNTGSVER
LKEIISTYGEDPMAGRIARAIVDARARNPIETTARLAEVVESAYPAKWRAKSRNHPATRTFQALRMAVNGELEELETFLA
AIVDRMNPGGRIVVITFHSLEDRLVKNAFRDEAKGCLCPRHIPVCVCGKKPRVNVLTRKPVTAGQAELQANSRASSAKLR
AAERI

Sequences:

>Translated_325_residues
MTADNGHDNNRHQYTAHVPVLLDEVLHYLSPVRGGRYLDGTLGLGGHSEAIMNRCGGDAWLLGLDRDREALAAASGRLAP
FGDRVTTRYACYSQFAAIMDEIGWTGLDGALIDIGVSSMQIDTPSRGFSFYADGPLDMRMDPSGDMPSAGVLVNTGSVER
LKEIISTYGEDPMAGRIARAIVDARARNPIETTARLAEVVESAYPAKWRAKSRNHPATRTFQALRMAVNGELEELETFLA
AIVDRMNPGGRIVVITFHSLEDRLVKNAFRDEAKGCLCPRHIPVCVCGKKPRVNVLTRKPVTAGQAELQANSRASSAKLR
AAERI
>Mature_324_residues
TADNGHDNNRHQYTAHVPVLLDEVLHYLSPVRGGRYLDGTLGLGGHSEAIMNRCGGDAWLLGLDRDREALAAASGRLAPF
GDRVTTRYACYSQFAAIMDEIGWTGLDGALIDIGVSSMQIDTPSRGFSFYADGPLDMRMDPSGDMPSAGVLVNTGSVERL
KEIISTYGEDPMAGRIARAIVDARARNPIETTARLAEVVESAYPAKWRAKSRNHPATRTFQALRMAVNGELEELETFLAA
IVDRMNPGGRIVVITFHSLEDRLVKNAFRDEAKGCLCPRHIPVCVCGKKPRVNVLTRKPVTAGQAELQANSRASSAKLRA
AERI

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=337, Percent_Identity=39.7626112759644, Blast_Score=207, Evalue=1e-53,
Organism=Homo sapiens, GI165377202, Length=197, Percent_Identity=43.6548223350254, Blast_Score=148, Evalue=8e-36,
Organism=Escherichia coli, GI1786270, Length=312, Percent_Identity=42.3076923076923, Blast_Score=219, Evalue=2e-58,
Organism=Drosophila melanogaster, GI62472493, Length=328, Percent_Identity=38.109756097561, Blast_Score=176, Evalue=2e-44,
Organism=Drosophila melanogaster, GI28571637, Length=328, Percent_Identity=38.109756097561, Blast_Score=176, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_DESDG (Q313R1)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_387530.1
- ProteinModelPortal:   Q313R1
- SMR:   Q313R1
- STRING:   Q313R1
- GeneID:   3755498
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_1034
- NMPDR:   fig|207559.3.peg.863
- eggNOG:   COG0275
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- ProtClustDB:   PRK00050
- BioCyc:   DDES207559:DDE_1034-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 35389; Mature: 35258

Theoretical pI: Translated: 7.86; Mature: 7.86

Prosite motif: NA

Important sites: BINDING 65-65 BINDING 92-92 BINDING 113-113 BINDING 120-120

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTADNGHDNNRHQYTAHVPVLLDEVLHYLSPVRGGRYLDGTLGLGGHSEAIMNRCGGDAW
CCCCCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCCEEECCCCCCCCCHHHHHHHCCCCEE
LLGLDRDREALAAASGRLAPFGDRVTTRYACYSQFAAIMDEIGWTGLDGALIDIGVSSMQ
EEECCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCEEE
IDTPSRGFSFYADGPLDMRMDPSGDMPSAGVLVNTGSVERLKEIISTYGEDPMAGRIARA
ECCCCCCCEEEECCCCCEEECCCCCCCCCCEEEECCCHHHHHHHHHHHCCCCHHHHHHHH
IVDARARNPIETTARLAEVVESAYPAKWRAKSRNHPATRTFQALRMAVNGELEELETFLA
HHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHH
AIVDRMNPGGRIVVITFHSLEDRLVKNAFRDEAKGCLCPRHIPVCVCGKKPRVNVLTRKP
HHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCEEEECCC
VTAGQAELQANSRASSAKLRAAERI
CCCCHHHHHCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
TADNGHDNNRHQYTAHVPVLLDEVLHYLSPVRGGRYLDGTLGLGGHSEAIMNRCGGDAW
CCCCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCCEEECCCCCCCCCHHHHHHHCCCCEE
LLGLDRDREALAAASGRLAPFGDRVTTRYACYSQFAAIMDEIGWTGLDGALIDIGVSSMQ
EEECCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCEEE
IDTPSRGFSFYADGPLDMRMDPSGDMPSAGVLVNTGSVERLKEIISTYGEDPMAGRIARA
ECCCCCCCEEEECCCCCEEECCCCCCCCCCEEEECCCHHHHHHHHHHHCCCCHHHHHHHH
IVDARARNPIETTARLAEVVESAYPAKWRAKSRNHPATRTFQALRMAVNGELEELETFLA
HHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHH
AIVDRMNPGGRIVVITFHSLEDRLVKNAFRDEAKGCLCPRHIPVCVCGKKPRVNVLTRKP
HHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCEEEECCC
VTAGQAELQANSRASSAKLRAAERI
CCCCHHHHHCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA