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The map label for this gene is grpE [H]

Identifier: 78356072

GI number: 78356072

Start: 1050141

End: 1050725

Strand: Reverse

Name: grpE [H]

Synonym: Dde_1025

Alternate gene names: 78356072

Gene position: 1050725-1050141 (Counterclockwise)

Preceding gene: 78356073

Following gene: 78356071

Centisome position: 28.17

GC content: 61.71

Gene sequence:

>585_bases
ATGGCGAACGACGAAACCCATGACATACCTGCAGAAAACGAAGAACTGGCTGCCGAAGCCGCCCGCGGTGCGGAGCAGGA
GGCGGCACAGGGCGCCGCTGACGCGCTGACCGGCGAAGAAGTGGCAGCCCTGTGCAGGGAACGAGTCTGTCCGGCATGTG
ACGAACACCAGAAGGCCGAAGACATCCGCCTGCGCGCACTGGCCGAGCTGGAAAACGTGAAAAAACGGCTGGAACGCGAA
CGTGAAGAACATCTGAAATATGCCGCGGAAAAAGTGCTTTCCGACCTGCTGCCCACTCTTGACCACCTTGACCTTGCCCT
GCAGTACGGTTCATCCGACCCCGCCTGCAAAAACATGGCAGTGGGTGTGGAAATGACCCGCAAGCTCTTTCTGGACGCGC
TGGCAGGTCACGGCCTGCAGCCCGTGGGCGAAAAAGGCCAGCCCTTTGACCCCGCTCTGCACGAGGCCGTAAGCAAGGAA
GAGGCTCCCGGCACGGAATCAGGCACCATCATCGCCGTCATGCAGCGTGGATACCGCCTCAAGGAGCGCCTGCTGCGCCC
CGCCAAAGTCACTGTCAGCGCCTGA

Upstream 100 bases:

>100_bases
ACCCTGCTGTTCTGAAACGTGCCCGCTCCGGCGGGGGCGTTCTGTACGCGCATCATGACGGCTATGCCGATGTTTTGAAT
ATAAAGGAGATTCTTCAAAA

Downstream 100 bases:

>100_bases
TCCGCGCTGTTCCGTCCGGTCACTTCCCGCAAAGACTCATACAGTTTCAACACCGCGGCCTGCGGTGCGCACTGACGCAC
CGCAGGCCGCGCTTTATTCC

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 194; Mature: 193

Protein sequence:

>194_residues
MANDETHDIPAENEELAAEAARGAEQEAAQGAADALTGEEVAALCRERVCPACDEHQKAEDIRLRALAELENVKKRLERE
REEHLKYAAEKVLSDLLPTLDHLDLALQYGSSDPACKNMAVGVEMTRKLFLDALAGHGLQPVGEKGQPFDPALHEAVSKE
EAPGTESGTIIAVMQRGYRLKERLLRPAKVTVSA

Sequences:

>Translated_194_residues
MANDETHDIPAENEELAAEAARGAEQEAAQGAADALTGEEVAALCRERVCPACDEHQKAEDIRLRALAELENVKKRLERE
REEHLKYAAEKVLSDLLPTLDHLDLALQYGSSDPACKNMAVGVEMTRKLFLDALAGHGLQPVGEKGQPFDPALHEAVSKE
EAPGTESGTIIAVMQRGYRLKERLLRPAKVTVSA
>Mature_193_residues
ANDETHDIPAENEELAAEAARGAEQEAAQGAADALTGEEVAALCRERVCPACDEHQKAEDIRLRALAELENVKKRLERER
EEHLKYAAEKVLSDLLPTLDHLDLALQYGSSDPACKNMAVGVEMTRKLFLDALAGHGLQPVGEKGQPFDPALHEAVSKEE
APGTESGTIIAVMQRGYRLKERLLRPAKVTVSA

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Homo sapiens, GI24308295, Length=134, Percent_Identity=35.8208955223881, Blast_Score=71, Evalue=7e-13,
Organism=Escherichia coli, GI1788967, Length=131, Percent_Identity=37.4045801526718, Blast_Score=100, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17552458, Length=136, Percent_Identity=36.7647058823529, Blast_Score=76, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6324806, Length=138, Percent_Identity=32.6086956521739, Blast_Score=84, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24653432, Length=161, Percent_Identity=34.1614906832298, Blast_Score=82, Evalue=2e-16,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 21117; Mature: 20986

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANDETHDIPAENEELAAEAARGAEQEAAQGAADALTGEEVAALCRERVCPACDEHQKAE
CCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHH
DIRLRALAELENVKKRLEREREEHLKYAAEKVLSDLLPTLDHLDLALQYGSSDPACKNMA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
VGVEMTRKLFLDALAGHGLQPVGEKGQPFDPALHEAVSKEEAPGTESGTIIAVMQRGYRL
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHCHHH
KERLLRPAKVTVSA
HHHCCCCCCEEECC
>Mature Secondary Structure 
ANDETHDIPAENEELAAEAARGAEQEAAQGAADALTGEEVAALCRERVCPACDEHQKAE
CCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHH
DIRLRALAELENVKKRLEREREEHLKYAAEKVLSDLLPTLDHLDLALQYGSSDPACKNMA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
VGVEMTRKLFLDALAGHGLQPVGEKGQPFDPALHEAVSKEEAPGTESGTIIAVMQRGYRL
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHCHHH
KERLLRPAKVTVSA
HHHCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA