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The map label for this gene is gatA

Identifier: 78356067

GI number: 78356067

Start: 1043225

End: 1044688

Strand: Reverse

Name: gatA

Synonym: Dde_1020

Alternate gene names: 78356067

Gene position: 1044688-1043225 (Counterclockwise)

Preceding gene: 78356068

Following gene: 78356066

Centisome position: 28.01

GC content: 60.66

Gene sequence:

>1464_bases
ATGGCCCAGATATACGCACAGACACTTACTGAAACCGCCGCCGCGCTGGCACAGGGCACACTGACAGCCGAAGAGGCCGT
CAGAGCATGCATAGACCGCATCGAAGCTACCGAACCGGCAGTGCACGCGCTGCTGGCCACCCGCTGCGAAGAAGCGCTGG
CCGAAGCCCGGGCCATGGACGCCGCGGGTTATGACCCCGCCAAACCCCTGTGGGGCGTGCCCGTCACCGTCAAGGATGTA
CTGAGCACCAGCGGTGTTGCCACCACCTGCGGCTCGCGCATACTGGAAAATTACGTGCCTTTTTTTGATGCCGCCGCCGT
GTCGCGCCTTAAAGATGCAGGTGCCGTCATTCTGGCAAAGACCAACATGGATGAATTCGCCATGGGGTCTTCCACCGAAA
AGTCGGCATTTAAAACCACCCACAACCCGTGGGACCTGCAAAGAGTACCCGGCGGTTCGTCCGGCGGATCTGCCGCTTCC
GTGGCCGCCGGTCAGTGTTTTGCATCGCTGGGTACCGATACCGGCGGCTCCGTGCGCCAGCCTGCCTCCTTCTGCGGATG
CGTGGGGCTGAAGCCCACATACGGCCGCGTTTCACGCTACGGGCTGGTGGCCTATGGTTCTTCGCTGGACCAGATAGGTC
CTGTCACCCGCAGCGTGGAAGACGCCGCCAGAGTGCTTGCCGTCATTGCCGGACACGATGCCCGCGACACCACATGTTCC
GACAGACCGGTGGATGATTATCTGGCCGCTCTCGGCTCCCGCAGCGACCTTGCCGGACTCCGTATAGGCGTACCGGCGGA
ATACTGGGGTGAAGGACTTTCCGGCGAGGTGGATTCCTGCTGCCGTGAAGCCCTGAAAAAAGCCGAAGAACTGGGTGCCA
CTCTGGTCGACATATCCCTGCCGAACAGCCGGCACGCCATTGCGGTGTACTACATCGTGGCCATGGCCGAAGCCAGCTCC
AATCTGGCCCGCTTCGACGGGGTGCGTTTCGGGCACCGCTCGGACAACGCGGCTTCACTGCCCGAACTGTACATAAACTC
CCGTTCCGAAGGATTCGGCGACGAAGTGCAGCGCCGCATCATGCTGGGCACCTATGTGCTTTCTTCCGGCTACTACGATG
CGTACTACCGCAAGGCCGCGCAGGTCCGCAGGCTTATTCTGCAGGATTTTGAAAAAGCCTTTGAGCAGTGCGATGTCATC
TGCGGCCCTGCTTCGCCCGTTACAGCATGGAAACACGGAGCCATGTCCGGCGACCCCCTGACCATGTATCTGCTGGATAT
TTTCACCATATCTCTCAACCTTGCGGGGCTGCCCGGTCTTTCTCTGCCTGTGGGTACGGGAACGGAATCAGGCATGCCTG
TGGGGCTGCAGATTCTGGGCAAGGCCTTTGACGAAGCTACATTGCTCTCTGTGGCCCATGTGCTTGAACAACGCATAGGC
CGCACCCCCGTGGCACAGATATAA

Upstream 100 bases:

>100_bases
CAATGCCCCCGAAGAAGACGGCACCTTCTTCATCGTGCCGCGCATTGTTTAATTCCTTCAGCAGACCGTAATCCAGAATC
ATACCCAAGGATGACCATTC

Downstream 100 bases:

>100_bases
CGCCGCATTTGCGGCAGGCGTCCGCACACGGGGCACCCGTTGCGGCCGCAGACAACCGGCAGGTATTCCGCACACGGCAC
GGCAAGCGTGCCGTGTGCGG

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 487; Mature: 486

Protein sequence:

>487_residues
MAQIYAQTLTETAAALAQGTLTAEEAVRACIDRIEATEPAVHALLATRCEEALAEARAMDAAGYDPAKPLWGVPVTVKDV
LSTSGVATTCGSRILENYVPFFDAAAVSRLKDAGAVILAKTNMDEFAMGSSTEKSAFKTTHNPWDLQRVPGGSSGGSAAS
VAAGQCFASLGTDTGGSVRQPASFCGCVGLKPTYGRVSRYGLVAYGSSLDQIGPVTRSVEDAARVLAVIAGHDARDTTCS
DRPVDDYLAALGSRSDLAGLRIGVPAEYWGEGLSGEVDSCCREALKKAEELGATLVDISLPNSRHAIAVYYIVAMAEASS
NLARFDGVRFGHRSDNAASLPELYINSRSEGFGDEVQRRIMLGTYVLSSGYYDAYYRKAAQVRRLILQDFEKAFEQCDVI
CGPASPVTAWKHGAMSGDPLTMYLLDIFTISLNLAGLPGLSLPVGTGTESGMPVGLQILGKAFDEATLLSVAHVLEQRIG
RTPVAQI

Sequences:

>Translated_487_residues
MAQIYAQTLTETAAALAQGTLTAEEAVRACIDRIEATEPAVHALLATRCEEALAEARAMDAAGYDPAKPLWGVPVTVKDV
LSTSGVATTCGSRILENYVPFFDAAAVSRLKDAGAVILAKTNMDEFAMGSSTEKSAFKTTHNPWDLQRVPGGSSGGSAAS
VAAGQCFASLGTDTGGSVRQPASFCGCVGLKPTYGRVSRYGLVAYGSSLDQIGPVTRSVEDAARVLAVIAGHDARDTTCS
DRPVDDYLAALGSRSDLAGLRIGVPAEYWGEGLSGEVDSCCREALKKAEELGATLVDISLPNSRHAIAVYYIVAMAEASS
NLARFDGVRFGHRSDNAASLPELYINSRSEGFGDEVQRRIMLGTYVLSSGYYDAYYRKAAQVRRLILQDFEKAFEQCDVI
CGPASPVTAWKHGAMSGDPLTMYLLDIFTISLNLAGLPGLSLPVGTGTESGMPVGLQILGKAFDEATLLSVAHVLEQRIG
RTPVAQI
>Mature_486_residues
AQIYAQTLTETAAALAQGTLTAEEAVRACIDRIEATEPAVHALLATRCEEALAEARAMDAAGYDPAKPLWGVPVTVKDVL
STSGVATTCGSRILENYVPFFDAAAVSRLKDAGAVILAKTNMDEFAMGSSTEKSAFKTTHNPWDLQRVPGGSSGGSAASV
AAGQCFASLGTDTGGSVRQPASFCGCVGLKPTYGRVSRYGLVAYGSSLDQIGPVTRSVEDAARVLAVIAGHDARDTTCSD
RPVDDYLAALGSRSDLAGLRIGVPAEYWGEGLSGEVDSCCREALKKAEELGATLVDISLPNSRHAIAVYYIVAMAEASSN
LARFDGVRFGHRSDNAASLPELYINSRSEGFGDEVQRRIMLGTYVLSSGYYDAYYRKAAQVRRLILQDFEKAFEQCDVIC
GPASPVTAWKHGAMSGDPLTMYLLDIFTISLNLAGLPGLSLPVGTGTESGMPVGLQILGKAFDEATLLSVAHVLEQRIGR
TPVAQI

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=510, Percent_Identity=37.843137254902, Blast_Score=331, Evalue=1e-90,
Organism=Homo sapiens, GI195972892, Length=499, Percent_Identity=27.2545090180361, Blast_Score=153, Evalue=4e-37,
Organism=Homo sapiens, GI166795287, Length=155, Percent_Identity=40, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17543272, Length=471, Percent_Identity=36.0934182590234, Blast_Score=269, Evalue=3e-72,
Organism=Caenorhabditis elegans, GI17537465, Length=500, Percent_Identity=27, Blast_Score=152, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI17556264, Length=187, Percent_Identity=37.4331550802139, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI71990152, Length=325, Percent_Identity=28.6153846153846, Blast_Score=108, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI17538252, Length=313, Percent_Identity=29.3929712460064, Blast_Score=95, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17538254, Length=339, Percent_Identity=26.8436578171091, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17556278, Length=195, Percent_Identity=35.3846153846154, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17556276, Length=195, Percent_Identity=35.3846153846154, Blast_Score=78, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323950, Length=422, Percent_Identity=34.5971563981043, Blast_Score=226, Evalue=6e-60,
Organism=Saccharomyces cerevisiae, GI6319685, Length=433, Percent_Identity=28.8683602771363, Blast_Score=114, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6320448, Length=533, Percent_Identity=26.6416510318949, Blast_Score=103, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24648113, Length=429, Percent_Identity=43.1235431235431, Blast_Score=310, Evalue=2e-84,
Organism=Drosophila melanogaster, GI24652985, Length=504, Percent_Identity=26.984126984127, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI19922090, Length=504, Percent_Identity=26.984126984127, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24652981, Length=504, Percent_Identity=26.984126984127, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24652983, Length=504, Percent_Identity=26.984126984127, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI45550774, Length=492, Percent_Identity=26.0162601626016, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24648435, Length=492, Percent_Identity=26.0162601626016, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24648437, Length=492, Percent_Identity=26.0162601626016, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24644968, Length=514, Percent_Identity=25.875486381323, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI161078093, Length=526, Percent_Identity=27.5665399239544, Blast_Score=125, Evalue=4e-29,
Organism=Drosophila melanogaster, GI21356731, Length=254, Percent_Identity=33.0708661417323, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI24648441, Length=425, Percent_Identity=25.4117647058824, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24648439, Length=425, Percent_Identity=25.4117647058824, Blast_Score=106, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_DESDG (Q313S5)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_387516.1
- ProteinModelPortal:   Q313S5
- SMR:   Q313S5
- STRING:   Q313S5
- GeneID:   3755522
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_1020
- NMPDR:   fig|207559.3.peg.39
- eggNOG:   COG0154
- HOGENOM:   HBG481888
- OMA:   TESSCYG
- ProtClustDB:   PRK00012
- BioCyc:   DDES207559:DDE_1020-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 51400; Mature: 51268

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 78-78 ACT_SITE 153-153 ACT_SITE 177-177

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQIYAQTLTETAAALAQGTLTAEEAVRACIDRIEATEPAVHALLATRCEEALAEARAMD
CCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
AAGYDPAKPLWGVPVTVKDVLSTSGVATTCGSRILENYVPFFDAAAVSRLKDAGAVILAK
CCCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCEEEEEE
TNMDEFAMGSSTEKSAFKTTHNPWDLQRVPGGSSGGSAASVAAGQCFASLGTDTGGSVRQ
CCCHHHHCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
PASFCGCVGLKPTYGRVSRYGLVAYGSSLDQIGPVTRSVEDAARVLAVIAGHDARDTTCS
CHHHHCCCCCCCCHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
DRPVDDYLAALGSRSDLAGLRIGVPAEYWGEGLSGEVDSCCREALKKAEELGATLVDISL
CCCHHHHHHHHCCCCCCCCEEECCCHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEC
PNSRHAIAVYYIVAMAEASSNLARFDGVRFGHRSDNAASLPELYINSRSEGFGDEVQRRI
CCCCCHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCHHHHCCCCCCCCHHHHHHH
MLGTYVLSSGYYDAYYRKAAQVRRLILQDFEKAFEQCDVICGPASPVTAWKHGAMSGDPL
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCHHHHCCCCCCCCH
TMYLLDIFTISLNLAGLPGLSLPVGTGTESGMPVGLQILGKAFDEATLLSVAHVLEQRIG
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
RTPVAQI
CCCCCCC
>Mature Secondary Structure 
AQIYAQTLTETAAALAQGTLTAEEAVRACIDRIEATEPAVHALLATRCEEALAEARAMD
CHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
AAGYDPAKPLWGVPVTVKDVLSTSGVATTCGSRILENYVPFFDAAAVSRLKDAGAVILAK
CCCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCEEEEEE
TNMDEFAMGSSTEKSAFKTTHNPWDLQRVPGGSSGGSAASVAAGQCFASLGTDTGGSVRQ
CCCHHHHCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
PASFCGCVGLKPTYGRVSRYGLVAYGSSLDQIGPVTRSVEDAARVLAVIAGHDARDTTCS
CHHHHCCCCCCCCHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
DRPVDDYLAALGSRSDLAGLRIGVPAEYWGEGLSGEVDSCCREALKKAEELGATLVDISL
CCCHHHHHHHHCCCCCCCCEEECCCHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEC
PNSRHAIAVYYIVAMAEASSNLARFDGVRFGHRSDNAASLPELYINSRSEGFGDEVQRRI
CCCCCHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCHHHHCCCCCCCCHHHHHHH
MLGTYVLSSGYYDAYYRKAAQVRRLILQDFEKAFEQCDVICGPASPVTAWKHGAMSGDPL
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCHHHHCCCCCCCCH
TMYLLDIFTISLNLAGLPGLSLPVGTGTESGMPVGLQILGKAFDEATLLSVAHVLEQRIG
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
RTPVAQI
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA