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The map label for this gene is glmU

Identifier: 78356028

GI number: 78356028

Start: 1008245

End: 1009627

Strand: Reverse

Name: glmU

Synonym: Dde_0981

Alternate gene names: 78356028

Gene position: 1009627-1008245 (Counterclockwise)

Preceding gene: 78356029

Following gene: 78356027

Centisome position: 27.07

GC content: 58.35

Gene sequence:

>1383_bases
ATGTCCTCGAACCAGTATACCGCAGGCGCTGTTATCCTTGCCGCAGGCAAAGGAACGCGCATGTATTCCGACAAACCCAA
GGTGCTGCAGCCGCTGCTGGGCGAGCCCATGCTGCGCTTTGTATACAGGGCGCTTGATCCGCTGTTTTCCAAGGCGGTAT
GGACTGTTATCGGCCATGGCGCAGACATGGTACGCAAAGCTTTCTGCCATGAAGACCGCGAATTCGTGCTGCAGGAAAAA
CAACTCGGCACCGGCCATGCGCTGCAGTGCGCATGGGACAAGGTCACAGCTTCCGGCATTGACTATGTGCTGGTCATCAA
CGGCGACACGCCGCTGGTGCGCCCCGATTCAATCGTCAGGCTGCTGGACCGCACGGCAGGCTCCGGCGCTGACGTGGGCT
TCATCACCCTGACGCTGGAAGACCCCGCAGCCTTCGGCAGGGTTGTACGCCGTCATGGGCACGTAGCAGCGGTCATTGAA
GCCAAAGACTACGATACGGCGCAGCACGGGCCGGAACCGCGTGAAATCAACGCAGGCATTTATCTGCTCAAGGTGGCCGC
AGTATCACCGCTGTTGCCTCTTCTGACCAATGCCAATGCCAGCGGAGAATACTACATTACCGATATCGTGGAACTTGCCG
TCAGGCAGGGACTGCGTGTGGAAGGTGTGGAATGCGGCAACGATCCTGACCTGCTGGGAGTGAACACTCCCGCCGAACTC
ATGCGTTCTGAAGAGCTGCTGCGCGAAAACATTGTGACCCGTCATCTGCACAACGGAGTCCATGTACATGCTGCCGGTTC
GGTACGCATCGGTCCCGATGTGGTCATAGAACCGGGGGCTGTCATTCACGGCCCGTGCGAACTGTACGGAAACACCTTTG
TGGGTGCTCAGGCCGTCATTGATTCGCACTGCTGGGTAAAAGACAGCCGTCTGCACCCCGGCAGCACCCTGCGCAATTTC
AGCCACGCAGAACAGGCGGAAATAGCCACAGGTGCCGTTGCCGGTCCATATGCCCGTCTGCGTCCCGGGGCCGTGCTGGA
ACAGGACGCCAGAATGGGCAACTTTGTTGAAATGAAAAAAGCCGTGCTCCGCAAAGGGGCAAAGGCAAGCCATCTGACCT
ATCTGGGTGATGCGGACATCGGCAGCGAAGCCAATATCGGCGCCGGCACCATCACCTGCAACTATGACGGCGTGAATAAA
CACCGCACCGTCATAGGCGAAAAAGCATTCATCGGCAGTAACACCGCGCTGGTGGCCCCCGTTTCCATCGGGAAGCAGGC
CCTTGTCGGCGCGGGATCAGTGATTACTAAAGACGTAGAGGACGGTGAATTGGCCATCGCCCGCGGACGTCAGAAAAACC
TGCGCAAAACGCGTCATTCTTGA

Upstream 100 bases:

>100_bases
TATTTATCTGGACAACGGGGCGTATCTGTCTGTAGTTTTCTTCTGTTTTAGCAGCTGTCCCATAACGGACAGCCAGACGA
GATACGCAACCGGAGACATC

Downstream 100 bases:

>100_bases
TTTCATGCATCAAAATGGTTACAATTCCGACATGGAGCTCTTAGACCGGCTGGAAAGCCGAATCGATTCACTACTTACTA
CCATTGAAGCGTTGAGCGAA

Product: bifunctional N-acetylglucosamine-1-phosphate uridyltransferase/glucosamine-1-phosphate acetyltransferase

Products: NA

Alternate protein names: UDP-N-acetylglucosamine pyrophosphorylase; N-acetylglucosamine-1-phosphate uridyltransferase; Glucosamine-1-phosphate N-acetyltransferase

Number of amino acids: Translated: 460; Mature: 459

Protein sequence:

>460_residues
MSSNQYTAGAVILAAGKGTRMYSDKPKVLQPLLGEPMLRFVYRALDPLFSKAVWTVIGHGADMVRKAFCHEDREFVLQEK
QLGTGHALQCAWDKVTASGIDYVLVINGDTPLVRPDSIVRLLDRTAGSGADVGFITLTLEDPAAFGRVVRRHGHVAAVIE
AKDYDTAQHGPEPREINAGIYLLKVAAVSPLLPLLTNANASGEYYITDIVELAVRQGLRVEGVECGNDPDLLGVNTPAEL
MRSEELLRENIVTRHLHNGVHVHAAGSVRIGPDVVIEPGAVIHGPCELYGNTFVGAQAVIDSHCWVKDSRLHPGSTLRNF
SHAEQAEIATGAVAGPYARLRPGAVLEQDARMGNFVEMKKAVLRKGAKASHLTYLGDADIGSEANIGAGTITCNYDGVNK
HRTVIGEKAFIGSNTALVAPVSIGKQALVGAGSVITKDVEDGELAIARGRQKNLRKTRHS

Sequences:

>Translated_460_residues
MSSNQYTAGAVILAAGKGTRMYSDKPKVLQPLLGEPMLRFVYRALDPLFSKAVWTVIGHGADMVRKAFCHEDREFVLQEK
QLGTGHALQCAWDKVTASGIDYVLVINGDTPLVRPDSIVRLLDRTAGSGADVGFITLTLEDPAAFGRVVRRHGHVAAVIE
AKDYDTAQHGPEPREINAGIYLLKVAAVSPLLPLLTNANASGEYYITDIVELAVRQGLRVEGVECGNDPDLLGVNTPAEL
MRSEELLRENIVTRHLHNGVHVHAAGSVRIGPDVVIEPGAVIHGPCELYGNTFVGAQAVIDSHCWVKDSRLHPGSTLRNF
SHAEQAEIATGAVAGPYARLRPGAVLEQDARMGNFVEMKKAVLRKGAKASHLTYLGDADIGSEANIGAGTITCNYDGVNK
HRTVIGEKAFIGSNTALVAPVSIGKQALVGAGSVITKDVEDGELAIARGRQKNLRKTRHS
>Mature_459_residues
SSNQYTAGAVILAAGKGTRMYSDKPKVLQPLLGEPMLRFVYRALDPLFSKAVWTVIGHGADMVRKAFCHEDREFVLQEKQ
LGTGHALQCAWDKVTASGIDYVLVINGDTPLVRPDSIVRLLDRTAGSGADVGFITLTLEDPAAFGRVVRRHGHVAAVIEA
KDYDTAQHGPEPREINAGIYLLKVAAVSPLLPLLTNANASGEYYITDIVELAVRQGLRVEGVECGNDPDLLGVNTPAELM
RSEELLRENIVTRHLHNGVHVHAAGSVRIGPDVVIEPGAVIHGPCELYGNTFVGAQAVIDSHCWVKDSRLHPGSTLRNFS
HAEQAEIATGAVAGPYARLRPGAVLEQDARMGNFVEMKKAVLRKGAKASHLTYLGDADIGSEANIGAGTITCNYDGVNKH
RTVIGEKAFIGSNTALVAPVSIGKQALVGAGSVITKDVEDGELAIARGRQKNLRKTRHS

Specific function: Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-GlcNAc. Responsible for the acetylation of Glc-N-1-P to give GlcNAc-1-P and for the uridyl transfer from UTP to GlcNAc-1-P which produces UDP-GlcNAc

COG id: COG1207

COG function: function code M; N-acetylglucosamine-1-phosphate uridyltransferase (contains nucleotidyltransferase and I-patch acetyltransferase domains)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transferase hexapeptide repeat family

Homologues:

Organism=Escherichia coli, GI1790168, Length=444, Percent_Identity=42.3423423423423, Blast_Score=326, Evalue=2e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLMU_DESDG (Q313W4)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_387477.1
- ProteinModelPortal:   Q313W4
- SMR:   Q313W4
- STRING:   Q313W4
- GeneID:   3755553
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_0981
- NMPDR:   fig|207559.3.peg.462
- eggNOG:   COG1207
- HOGENOM:   HBG688195
- OMA:   MERTCLA
- ProtClustDB:   PRK14356
- BioCyc:   DDES207559:DDE_0981-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01631
- InterPro:   IPR005882
- InterPro:   IPR018357
- InterPro:   IPR005835
- InterPro:   IPR011004
- TIGRFAMs:   TIGR01173

Pfam domain/function: PF00483 NTP_transferase; SSF51161 Trimer_LpxA_like

EC number: =2.7.7.23; =2.3.1.157

Molecular weight: Translated: 49298; Mature: 49167

Theoretical pI: Translated: 7.29; Mature: 7.29

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: ACT_SITE 371-371 BINDING 78-78 BINDING 146-146 BINDING 160-160 BINDING 177-177 BINDING 395-395 BINDING 413-413 BINDING 431-431 BINDING 448-448

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSNQYTAGAVILAAGKGTRMYSDKPKVLQPLLGEPMLRFVYRALDPLFSKAVWTVIGHG
CCCCCEECCEEEEEECCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCC
ADMVRKAFCHEDREFVLQEKQLGTGHALQCAWDKVTASGIDYVLVINGDTPLVRPDSIVR
HHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEHHHHHCCCCCEEEEECCCCCCCCHHHHHH
LLDRTAGSGADVGFITLTLEDPAAFGRVVRRHGHVAAVIEAKDYDTAQHGPEPREINAGI
HHHHCCCCCCCEEEEEEEECCHHHHHHHHHHCCCEEEEEEECCCCCHHCCCCCCEECCCE
YLLKVAAVSPLLPLLTNANASGEYYITDIVELAVRQGLRVEGVECGNDPDLLGVNTPAEL
EEEEHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHCCCEEECEECCCCCCEEECCCHHHH
MRSEELLRENIVTRHLHNGVHVHAAGSVRIGPDVVIEPGAVIHGPCELYGNTFVGAQAVI
HHHHHHHHHHHHHHHHCCCEEEEECCCEEECCCEEECCCCEEECHHHHCCCCCCCHHHHH
DSHCWVKDSRLHPGSTLRNFSHAEQAEIATGAVAGPYARLRPGAVLEQDARMGNFVEMKK
HCCCEEECCCCCCCHHHHHCCHHHHHHHHCCCCCCCHHHCCCCCHHHHHHCCCHHHHHHH
AVLRKGAKASHLTYLGDADIGSEANIGAGTITCNYDGVNKHRTVIGEKAFIGSNTALVAP
HHHHCCCCCCEEEEECCCCCCCCCCCCCEEEEEECCCCCCHHHEECCEEEECCCCEEEEE
VSIGKQALVGAGSVITKDVEDGELAIARGRQKNLRKTRHS
HHCCCHHHHCCCCEEECCCCCCCEEEECCCHHHHHHHCCC
>Mature Secondary Structure 
SSNQYTAGAVILAAGKGTRMYSDKPKVLQPLLGEPMLRFVYRALDPLFSKAVWTVIGHG
CCCCEECCEEEEEECCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCC
ADMVRKAFCHEDREFVLQEKQLGTGHALQCAWDKVTASGIDYVLVINGDTPLVRPDSIVR
HHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEHHHHHCCCCCEEEEECCCCCCCCHHHHHH
LLDRTAGSGADVGFITLTLEDPAAFGRVVRRHGHVAAVIEAKDYDTAQHGPEPREINAGI
HHHHCCCCCCCEEEEEEEECCHHHHHHHHHHCCCEEEEEEECCCCCHHCCCCCCEECCCE
YLLKVAAVSPLLPLLTNANASGEYYITDIVELAVRQGLRVEGVECGNDPDLLGVNTPAEL
EEEEHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHCCCEEECEECCCCCCEEECCCHHHH
MRSEELLRENIVTRHLHNGVHVHAAGSVRIGPDVVIEPGAVIHGPCELYGNTFVGAQAVI
HHHHHHHHHHHHHHHHCCCEEEEECCCEEECCCEEECCCCEEECHHHHCCCCCCCHHHHH
DSHCWVKDSRLHPGSTLRNFSHAEQAEIATGAVAGPYARLRPGAVLEQDARMGNFVEMKK
HCCCEEECCCCCCCHHHHHCCHHHHHHHHCCCCCCCHHHCCCCCHHHHHHCCCHHHHHHH
AVLRKGAKASHLTYLGDADIGSEANIGAGTITCNYDGVNKHRTVIGEKAFIGSNTALVAP
HHHHCCCCCCEEEEECCCCCCCCCCCCCEEEEEECCCCCCHHHEECCEEEECCCCEEEEE
VSIGKQALVGAGSVITKDVEDGELAIARGRQKNLRKTRHS
HHCCCHHHHCCCCEEECCCCCCCEEEECCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA