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The map label for this gene is hphIBM [H]

Identifier: 78355969

GI number: 78355969

Start: 950735

End: 953131

Strand: Direct

Name: hphIBM [H]

Synonym: Dde_0922

Alternate gene names: 78355969

Gene position: 950735-953131 (Clockwise)

Preceding gene: 78355968

Following gene: 78355970

Centisome position: 25.49

GC content: 62.16

Gene sequence:

>2397_bases
ATGGAACTGTTCGCCACAGACCTGGAAAGGCTGGCGTTTCTACTGGAGGCCGATGCGGCGCTGACTCTCGATCCCGATGC
GCTCGGGACCGAGGCCGCCGAACAGTCCGCTCCTGAAGAGCTCCCTCCCGAGAAGCGCCCCAAGTACATCACCAACTACA
TCGGCAGTAAGCAGAAGCTCGTCGACTGGATCTGGAAGCATACCCCGGAAGGCGTTGGCACCGTGCTCGACGCCTTTTCG
GGGTCTGCGGTCGTGGCCTACATGTACAAGACCAAGGGCCTCCAGGTCATCGCCAACGACCGGCTCCGCTACTGCCACCA
CGCCGCCAAGGCGATCATCGAGAACAACTCGGTTCGCCTGAGCGAGGACGAGATCGAGGCGCTCCTGGCCGACAACGCCA
AGGCGGGCAGCTTCGTTCAGGACAACTTCAAGGGCATTTTCTTCGCCAAGGGCGTCCATGCGCTGATCGACACCATCCGC
GCCAACTGCGACAAGCTCTCCGGCTTCAAGAAAGACATCGCCCTGTTCGGCCTCGGCAAGACCTGCATGAGCGGCAAGGG
CGGCTTCGGCCACTTCTCGTCGTCCACCGACTATGGCCGCCGCCAGGACACCCCAGACGAATTCAAGGATCGTCTGCGCA
AGAACCTGCAGCGCATCAACGCCCTGGTCTTCGACAACGACAAGGAGAACAAGGCCCACCGGCAGGACATCAACGACCTG
CTGCCGAAAGCCAAGGCGGATCTGGCCTACTTCGATCCGCCCTACGCCACCGAGTTTTCGACTACCAACTACGAGCGGGC
CTACCACTTCGTGGAAGGGCTCATGACCTATTGGGAAGGGCTCGAAATCAAGGCCGACACCAAGGTCAAGTATTACGAGA
CCGACCACAAGACCGTCACCAAGGCCAACGCCAGCGAGTTCTTCCAGACCTTTCTCGGCAATGCCAAGCACATTCCGCAC
TGGCTGATTTCCTACCGCGATCACGCCTATCCCAACGAGCAGGAGATGAAGCGGATCATCGGCTCCTTCGGCAAACAGAG
CCGGATGAAGTCCAAGGATCACCACTACGCCATCACCTCCAAGCACGGCGAGGCTTCCAACGCCAAAGAACGTCTGTTCG
TCTGCGCTCCCGGGGCCAAGGCCAGCGCCGAACGGGAGGAGAAACCCGTTCCGATGGCCGCCGCCGCGAACTTCCACACC
AGCATCCCCGTGGACATCCGGCTGGGCGATGGTGAACGCCTCGCGACCGAGGCCATGGATGTCGGTTCGGCGGGCGACCC
CCAGTTCAGCTTCGTGCTCTGCCGAACCGGGACCAACAAGAACGGCGACCACTTCACCGCCGAGGAGTTGTCCGGTCGGC
ACATGACGGCCGTGAACAAGAAGGTCGATCTGCAGCATTCGCAGGAGTTCAACGACATCGTGGGTGGCATCGTCGCCGCC
GACTACCTGGAGGACGACAACGGCGGCCGCGTGGAATGCGTCGGCGAGCTGTATGTCCACGACACCCCGGCCGCCCGGCT
GGCCTACAAGCTGATGAAGCGCGGGATCATCTCCCAGGTCTCCATGGAGTGCGACTACCAGGAAGGCGAATGCTCGGTCT
GCCACAAGCGCTTCCAGAACAAGGCCGACTACTGCACCCACCTGCGCAAATTCAAGGGCCGTGATGTGGGCGGCCAACCC
GTTTTCGAGATTCTGCACGGCGTCACTTTCACCGGACTGGGACTGCTCGACCGCAAAGGCGCGGACGAGAACGCCAGGAT
TCTGCAGGTGGCGTCCCTTCAGAGCCAGCCCGACCAATCCCAACCCGAAGGAGATTCCACGATGGAAGACAAAACCAAAC
CTACAGATGAACCGGCCGTCGAAGCGGCCAAGAAGAAACCGGCCCAGCAGGAAGGCGATCCTGCCCGCGTTACCGACCTG
GAAAAGGAAAACCGGCAGCTCAAGGCCCAGGTGGCCGAGCTGCAGAAACGAGTCCAGGAACTGGAAGCCGAACAAAAGGC
GGCCGCCTGCCGCTCCCGGGCCAAGAAGCTGCTCACCCGTCTGGAGAAGCAGGGGCTCTCCTTCGCTTCCGAGGAGGACC
GGGAAGCCGAGCTGAAACGCCTGGCCGAACTGTCCGACGAAGCCTTCGCCGCCACCGAGGCCGCTTACGAACGCCTGCCC
AAATCGGCCAAGGCGGACAAGGACAAGGAAGAGAAACCCGCCGACAGCGACCAGGGCGGCAAGCCCGCCGCCAAGGCATC
GACGGAAACCCCGCTACGCAGCGACGCCGGTGTCCGGCCCCACGATGTGGACGACCGCAAGGTGTCGCTCGAGGACCGTC
TGCGCGACGGGTTCATGGCCGCTTACCGCAACCGTGTCGGCGAGGACTTTCCCGAACACTCGCAAATCAACGCATAA

Upstream 100 bases:

>100_bases
TCTCCGTCACCCTCCGACACATCAACGACGCTTCCGGTAAGTAACCGCTGAACGCTCCCCGCAGGTCGCGGAGAGCACAG
CAAACTGACCGGAGACGTTG

Downstream 100 bases:

>100_bases
GGAGGAAACACCATGTCATTCATCAATCCGTGTCACCGCAGCCTCGCCTATGGCGACGGCCACATCCAGGGCGACGGCCA
ACTCGGCCAGGTGGTCCGCG

Product: adenine-specific DNA methylase-like protein

Products: NA

Alternate protein names: M.HphIB; Adenine-specific methyltransferase HphIB; M.HphI(A) [H]

Number of amino acids: Translated: 798; Mature: 798

Protein sequence:

>798_residues
MELFATDLERLAFLLEADAALTLDPDALGTEAAEQSAPEELPPEKRPKYITNYIGSKQKLVDWIWKHTPEGVGTVLDAFS
GSAVVAYMYKTKGLQVIANDRLRYCHHAAKAIIENNSVRLSEDEIEALLADNAKAGSFVQDNFKGIFFAKGVHALIDTIR
ANCDKLSGFKKDIALFGLGKTCMSGKGGFGHFSSSTDYGRRQDTPDEFKDRLRKNLQRINALVFDNDKENKAHRQDINDL
LPKAKADLAYFDPPYATEFSTTNYERAYHFVEGLMTYWEGLEIKADTKVKYYETDHKTVTKANASEFFQTFLGNAKHIPH
WLISYRDHAYPNEQEMKRIIGSFGKQSRMKSKDHHYAITSKHGEASNAKERLFVCAPGAKASAEREEKPVPMAAAANFHT
SIPVDIRLGDGERLATEAMDVGSAGDPQFSFVLCRTGTNKNGDHFTAEELSGRHMTAVNKKVDLQHSQEFNDIVGGIVAA
DYLEDDNGGRVECVGELYVHDTPAARLAYKLMKRGIISQVSMECDYQEGECSVCHKRFQNKADYCTHLRKFKGRDVGGQP
VFEILHGVTFTGLGLLDRKGADENARILQVASLQSQPDQSQPEGDSTMEDKTKPTDEPAVEAAKKKPAQQEGDPARVTDL
EKENRQLKAQVAELQKRVQELEAEQKAAACRSRAKKLLTRLEKQGLSFASEEDREAELKRLAELSDEAFAATEAAYERLP
KSAKADKDKEEKPADSDQGGKPAAKASTETPLRSDAGVRPHDVDDRKVSLEDRLRDGFMAAYRNRVGEDFPEHSQINA

Sequences:

>Translated_798_residues
MELFATDLERLAFLLEADAALTLDPDALGTEAAEQSAPEELPPEKRPKYITNYIGSKQKLVDWIWKHTPEGVGTVLDAFS
GSAVVAYMYKTKGLQVIANDRLRYCHHAAKAIIENNSVRLSEDEIEALLADNAKAGSFVQDNFKGIFFAKGVHALIDTIR
ANCDKLSGFKKDIALFGLGKTCMSGKGGFGHFSSSTDYGRRQDTPDEFKDRLRKNLQRINALVFDNDKENKAHRQDINDL
LPKAKADLAYFDPPYATEFSTTNYERAYHFVEGLMTYWEGLEIKADTKVKYYETDHKTVTKANASEFFQTFLGNAKHIPH
WLISYRDHAYPNEQEMKRIIGSFGKQSRMKSKDHHYAITSKHGEASNAKERLFVCAPGAKASAEREEKPVPMAAAANFHT
SIPVDIRLGDGERLATEAMDVGSAGDPQFSFVLCRTGTNKNGDHFTAEELSGRHMTAVNKKVDLQHSQEFNDIVGGIVAA
DYLEDDNGGRVECVGELYVHDTPAARLAYKLMKRGIISQVSMECDYQEGECSVCHKRFQNKADYCTHLRKFKGRDVGGQP
VFEILHGVTFTGLGLLDRKGADENARILQVASLQSQPDQSQPEGDSTMEDKTKPTDEPAVEAAKKKPAQQEGDPARVTDL
EKENRQLKAQVAELQKRVQELEAEQKAAACRSRAKKLLTRLEKQGLSFASEEDREAELKRLAELSDEAFAATEAAYERLP
KSAKADKDKEEKPADSDQGGKPAAKASTETPLRSDAGVRPHDVDDRKVSLEDRLRDGFMAAYRNRVGEDFPEHSQINA
>Mature_798_residues
MELFATDLERLAFLLEADAALTLDPDALGTEAAEQSAPEELPPEKRPKYITNYIGSKQKLVDWIWKHTPEGVGTVLDAFS
GSAVVAYMYKTKGLQVIANDRLRYCHHAAKAIIENNSVRLSEDEIEALLADNAKAGSFVQDNFKGIFFAKGVHALIDTIR
ANCDKLSGFKKDIALFGLGKTCMSGKGGFGHFSSSTDYGRRQDTPDEFKDRLRKNLQRINALVFDNDKENKAHRQDINDL
LPKAKADLAYFDPPYATEFSTTNYERAYHFVEGLMTYWEGLEIKADTKVKYYETDHKTVTKANASEFFQTFLGNAKHIPH
WLISYRDHAYPNEQEMKRIIGSFGKQSRMKSKDHHYAITSKHGEASNAKERLFVCAPGAKASAEREEKPVPMAAAANFHT
SIPVDIRLGDGERLATEAMDVGSAGDPQFSFVLCRTGTNKNGDHFTAEELSGRHMTAVNKKVDLQHSQEFNDIVGGIVAA
DYLEDDNGGRVECVGELYVHDTPAARLAYKLMKRGIISQVSMECDYQEGECSVCHKRFQNKADYCTHLRKFKGRDVGGQP
VFEILHGVTFTGLGLLDRKGADENARILQVASLQSQPDQSQPEGDSTMEDKTKPTDEPAVEAAKKKPAQQEGDPARVTDL
EKENRQLKAQVAELQKRVQELEAEQKAAACRSRAKKLLTRLEKQGLSFASEEDREAELKRLAELSDEAFAATEAAYERLP
KSAKADKDKEEKPADSDQGGKPAAKASTETPLRSDAGVRPHDVDDRKVSLEDRLRDGFMAAYRNRVGEDFPEHSQINA

Specific function: This methylase protects the DNA from cleavage by the HphI endonuclease [H]

COG id: COG3392

COG function: function code L; Adenine-specific DNA methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR012327 [H]

Pfam domain/function: PF02086 MethyltransfD12 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 88875; Mature: 88875

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELFATDLERLAFLLEADAALTLDPDALGTEAAEQSAPEELPPEKRPKYITNYIGSKQKL
CCCHHHHHHHHHHHHHCCCEEEECCHHHCCHHHHHCCCCCCCCCCCCHHHHHHHCCHHHH
VDWIWKHTPEGVGTVLDAFSGSAVVAYMYKTKGLQVIANDRLRYCHHAAKAIIENNSVRL
HHHHHHCCCCHHHHHHHHCCCCEEEEEEECCCCEEEEECHHHHHHHHHHHHHHCCCCEEE
SEDEIEALLADNAKAGSFVQDNFKGIFFAKGVHALIDTIRANCDKLSGFKKDIALFGLGK
CHHHHHHHHHCCCCCCCHHHHCCCEEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHEECCH
TCMSGKGGFGHFSSSTDYGRRQDTPDEFKDRLRKNLQRINALVFDNDKENKAHRQDINDL
HHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHH
LPKAKADLAYFDPPYATEFSTTNYERAYHFVEGLMTYWEGLEIKADTKVKYYETDHKTVT
CCHHHCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCEEEEEECCCCHHH
KANASEFFQTFLGNAKHIPHWLISYRDHAYPNEQEMKRIIGSFGKQSRMKSKDHHYAITS
HCCHHHHHHHHHCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHCHHHHCCCCCCCEEEEC
KHGEASNAKERLFVCAPGAKASAEREEKPVPMAAAANFHTSIPVDIRLGDGERLATEAMD
CCCCCCCCCCEEEEECCCCCCCCHHHCCCCCEEHHCCCCCCCCEEEEECCCCHHHHHHHH
VGSAGDPQFSFVLCRTGTNKNGDHFTAEELSGRHMTAVNKKVDLQHSQEFNDIVGGIVAA
CCCCCCCCEEEEEEECCCCCCCCCEEHHHHCCCEEEHHCCHHCCHHHHHHHHHHHHHHHH
DYLEDDNGGRVECVGELYVHDTPAARLAYKLMKRGIISQVSMECDYQEGECSVCHKRFQN
HHHCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHC
KADYCTHLRKFKGRDVGGQPVFEILHGVTFTGLGLLDRKGADENARILQVASLQSQPDQS
HHHHHHHHHHHCCCCCCCCHHHHHHHCCHHHCCCHHHCCCCCCCCCEEEEHHHCCCCCCC
QPEGDSTMEDKTKPTDEPAVEAAKKKPAQQEGDPARVTDLEKENRQLKAQVAELQKRVQE
CCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHH
LEAEQKAAACRSRAKKLLTRLEKQGLSFASEEDREAELKRLAELSDEAFAATEAAYERLP
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KSAKADKDKEEKPADSDQGGKPAAKASTETPLRSDAGVRPHDVDDRKVSLEDRLRDGFMA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECHHHHHHHHHHH
AYRNRVGEDFPEHSQINA
HHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MELFATDLERLAFLLEADAALTLDPDALGTEAAEQSAPEELPPEKRPKYITNYIGSKQKL
CCCHHHHHHHHHHHHHCCCEEEECCHHHCCHHHHHCCCCCCCCCCCCHHHHHHHCCHHHH
VDWIWKHTPEGVGTVLDAFSGSAVVAYMYKTKGLQVIANDRLRYCHHAAKAIIENNSVRL
HHHHHHCCCCHHHHHHHHCCCCEEEEEEECCCCEEEEECHHHHHHHHHHHHHHCCCCEEE
SEDEIEALLADNAKAGSFVQDNFKGIFFAKGVHALIDTIRANCDKLSGFKKDIALFGLGK
CHHHHHHHHHCCCCCCCHHHHCCCEEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHEECCH
TCMSGKGGFGHFSSSTDYGRRQDTPDEFKDRLRKNLQRINALVFDNDKENKAHRQDINDL
HHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHH
LPKAKADLAYFDPPYATEFSTTNYERAYHFVEGLMTYWEGLEIKADTKVKYYETDHKTVT
CCHHHCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCEEEEEECCCCHHH
KANASEFFQTFLGNAKHIPHWLISYRDHAYPNEQEMKRIIGSFGKQSRMKSKDHHYAITS
HCCHHHHHHHHHCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHCHHHHCCCCCCCEEEEC
KHGEASNAKERLFVCAPGAKASAEREEKPVPMAAAANFHTSIPVDIRLGDGERLATEAMD
CCCCCCCCCCEEEEECCCCCCCCHHHCCCCCEEHHCCCCCCCCEEEEECCCCHHHHHHHH
VGSAGDPQFSFVLCRTGTNKNGDHFTAEELSGRHMTAVNKKVDLQHSQEFNDIVGGIVAA
CCCCCCCCEEEEEEECCCCCCCCCEEHHHHCCCEEEHHCCHHCCHHHHHHHHHHHHHHHH
DYLEDDNGGRVECVGELYVHDTPAARLAYKLMKRGIISQVSMECDYQEGECSVCHKRFQN
HHHCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHC
KADYCTHLRKFKGRDVGGQPVFEILHGVTFTGLGLLDRKGADENARILQVASLQSQPDQS
HHHHHHHHHHHCCCCCCCCHHHHHHHCCHHHCCCHHHCCCCCCCCCEEEEHHHCCCCCCC
QPEGDSTMEDKTKPTDEPAVEAAKKKPAQQEGDPARVTDLEKENRQLKAQVAELQKRVQE
CCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHH
LEAEQKAAACRSRAKKLLTRLEKQGLSFASEEDREAELKRLAELSDEAFAATEAAYERLP
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KSAKADKDKEEKPADSDQGGKPAAKASTETPLRSDAGVRPHDVDDRKVSLEDRLRDGFMA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECHHHHHHHHHHH
AYRNRVGEDFPEHSQINA
HHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8759008 [H]