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The map label for this gene is dosC [H]

Identifier: 78355933

GI number: 78355933

Start: 915787

End: 917415

Strand: Reverse

Name: dosC [H]

Synonym: Dde_0886

Alternate gene names: 78355933

Gene position: 917415-915787 (Counterclockwise)

Preceding gene: 78355937

Following gene: 78355932

Centisome position: 24.59

GC content: 57.21

Gene sequence:

>1629_bases
ATGGCACGCAGTCAGAGCATTGCTTCACGTATCCGTTTTTTTTCCTTTCTGCTTGTAACGCTGCCTCTTTGCGTGTCTCT
GCTCACGTTTGCTGTTGTCAGCCGCATTCTGGTTGTGGGCGGTGCGCACCATGACATGGTGGTGGAGCTGCAGCACCACA
AACTGACCACCGAAAGATGGCTTAACGAGCAACTGCAAAGCATCACATTTTTTGCCCATACCGGCATCAGCGACCTGCAC
GATACAGCCGCGCTGCAACAGGCTTTGCGCGTTTTTCTTAATTACCACACATCGTTCAGTCAGGTTTTTTACGCCTCTCC
CGATGGCTCACTGCTGACAGGGCCGGAACTGCCCGTGCCGATCAATGTAGGTGACCGCGGCTACTTCCAAAAAGCCCGTC
AGGGCCTGCCCGTGGTTTCTCCGGTGCTGGTAAGCCGCTTTTCGCACAACCCGTCGCTTATTTTCTGCGCGCCGCTGCCT
TTTGCCGACGGCCGCTTTGCAGGAGCTGTCATCGGCGTGACAGACCTGCGCACCCTTGACGAGGCCCTGCTGCGCCTGCA
CCGCCGCCAGAACGATCAAAGCTTTCTGGCCACTGCCGACGGATACCTGCTGACCAGCAGACTGCAGGAAAACACCATCA
TCCTGCCGCGCTATAATCCGGAAGGCATTCCCGTCACGCGAACCGTACTGCCGCCCGAAGATTCGCTGAGCACACCGCAT
TTTTACCGCAACACTCAGGGCGTGTATGTGCTGGGCGTCAGACTGGCTTTCAAATCCGGCCAATGGCTGCTGGTGCAGGA
AAGACCAGTGCGTGCCATGCTGGGCGGATACAGCTGGATAATTGCCATGACCATGGCGGGCGCTCTGGCCACCGCAGCCC
TGCTCATCCCGTTTCTGCTCAAAGCGGGCAGACGCATAACCGGCCCCATAGAAGAGATATCAACCATGTCGGAAGAGCTG
ACCCACGGGCACTACGACAAGGAATGTCCCTATCTGCACACCGCCGATATGCCGCCTGAAATACGCAGACTGTACGACAA
CTTCTGCTTTATGGCGGAACGCGTTTCACAGCAGATGCGCGATCTGGAGCATCTTTCTTTCTGCGACACGCTGACCGGCC
TGCACAACAGGCGGCATCTGGCGCACGAAGGAACCAGAACCATCACAGCCTGCACCGAAGCCGACCTGCCGTGCAGCTGT
CTGATGCTCGACATTGATTTTTTTAAACAGGTCAACGATGCGTGGGGGCACGATGCGGGAGACATGGTGCTGCAGTCCGT
GGCACGGACCATCTCGAACGCGGTACGCAGCAAAGACATGACCATCCGCATGGGCGGCGAAGAGTTCGCCGTCATTGCCC
CTGCCACCGACACGGCATCGGCCTTTTCACTGGCGGAACGTATCCGCAGAAATATTGAATCGACCCCCGTTCAGGCTGCC
GACGATGAAATACGCATAACCATCAGCATCGGTGTGGCCGATATGGGCGGGGCACCCATCTTCGGCACCACGCAGCTGGA
CGACCTGCTGGCAAAAGCCGACAGGGCCTTGTATCAGGCAAAGAAAAAAGGCAGAAACAGAACAGAAATCTGGTCTGAAA
AACTCATTGCTGCCGCTCCGGACGGCTGA

Upstream 100 bases:

>100_bases
TCTTAATCACGCTCCGGCCGTTGCTTGCGGCATGACCATCTGATATTCTGAAAGGCCGAATGGTTATAGCAGCCTGCAAC
CTTTAACGGTACAAGGCGTC

Downstream 100 bases:

>100_bases
CGCCGCAGCAACCCTTGACAGCGGGCAGCCGATTGGCTAATAAAAATCAATACTATTTAAGGAGAGTCTCATGGAAGATA
AAGTTCTGAAAGCAATGCAG

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 542; Mature: 541

Protein sequence:

>542_residues
MARSQSIASRIRFFSFLLVTLPLCVSLLTFAVVSRILVVGGAHHDMVVELQHHKLTTERWLNEQLQSITFFAHTGISDLH
DTAALQQALRVFLNYHTSFSQVFYASPDGSLLTGPELPVPINVGDRGYFQKARQGLPVVSPVLVSRFSHNPSLIFCAPLP
FADGRFAGAVIGVTDLRTLDEALLRLHRRQNDQSFLATADGYLLTSRLQENTIILPRYNPEGIPVTRTVLPPEDSLSTPH
FYRNTQGVYVLGVRLAFKSGQWLLVQERPVRAMLGGYSWIIAMTMAGALATAALLIPFLLKAGRRITGPIEEISTMSEEL
THGHYDKECPYLHTADMPPEIRRLYDNFCFMAERVSQQMRDLEHLSFCDTLTGLHNRRHLAHEGTRTITACTEADLPCSC
LMLDIDFFKQVNDAWGHDAGDMVLQSVARTISNAVRSKDMTIRMGGEEFAVIAPATDTASAFSLAERIRRNIESTPVQAA
DDEIRITISIGVADMGGAPIFGTTQLDDLLAKADRALYQAKKKGRNRTEIWSEKLIAAAPDG

Sequences:

>Translated_542_residues
MARSQSIASRIRFFSFLLVTLPLCVSLLTFAVVSRILVVGGAHHDMVVELQHHKLTTERWLNEQLQSITFFAHTGISDLH
DTAALQQALRVFLNYHTSFSQVFYASPDGSLLTGPELPVPINVGDRGYFQKARQGLPVVSPVLVSRFSHNPSLIFCAPLP
FADGRFAGAVIGVTDLRTLDEALLRLHRRQNDQSFLATADGYLLTSRLQENTIILPRYNPEGIPVTRTVLPPEDSLSTPH
FYRNTQGVYVLGVRLAFKSGQWLLVQERPVRAMLGGYSWIIAMTMAGALATAALLIPFLLKAGRRITGPIEEISTMSEEL
THGHYDKECPYLHTADMPPEIRRLYDNFCFMAERVSQQMRDLEHLSFCDTLTGLHNRRHLAHEGTRTITACTEADLPCSC
LMLDIDFFKQVNDAWGHDAGDMVLQSVARTISNAVRSKDMTIRMGGEEFAVIAPATDTASAFSLAERIRRNIESTPVQAA
DDEIRITISIGVADMGGAPIFGTTQLDDLLAKADRALYQAKKKGRNRTEIWSEKLIAAAPDG
>Mature_541_residues
ARSQSIASRIRFFSFLLVTLPLCVSLLTFAVVSRILVVGGAHHDMVVELQHHKLTTERWLNEQLQSITFFAHTGISDLHD
TAALQQALRVFLNYHTSFSQVFYASPDGSLLTGPELPVPINVGDRGYFQKARQGLPVVSPVLVSRFSHNPSLIFCAPLPF
ADGRFAGAVIGVTDLRTLDEALLRLHRRQNDQSFLATADGYLLTSRLQENTIILPRYNPEGIPVTRTVLPPEDSLSTPHF
YRNTQGVYVLGVRLAFKSGQWLLVQERPVRAMLGGYSWIIAMTMAGALATAALLIPFLLKAGRRITGPIEEISTMSEELT
HGHYDKECPYLHTADMPPEIRRLYDNFCFMAERVSQQMRDLEHLSFCDTLTGLHNRRHLAHEGTRTITACTEADLPCSCL
MLDIDFFKQVNDAWGHDAGDMVLQSVARTISNAVRSKDMTIRMGGEEFAVIAPATDTASAFSLAERIRRNIESTPVQAAD
DEIRITISIGVADMGGAPIFGTTQLDDLLAKADRALYQAKKKGRNRTEIWSEKLIAAAPDG

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=169, Percent_Identity=39.6449704142012, Blast_Score=129, Evalue=5e-31,
Organism=Escherichia coli, GI87081881, Length=236, Percent_Identity=34.7457627118644, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1787802, Length=180, Percent_Identity=34.4444444444444, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1786584, Length=179, Percent_Identity=34.0782122905028, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI87082007, Length=188, Percent_Identity=37.7659574468085, Blast_Score=112, Evalue=8e-26,
Organism=Escherichia coli, GI1787262, Length=174, Percent_Identity=35.0574712643678, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1788085, Length=184, Percent_Identity=30.9782608695652, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1787816, Length=169, Percent_Identity=33.7278106508876, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI87081977, Length=157, Percent_Identity=31.2101910828025, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI87081974, Length=163, Percent_Identity=28.2208588957055, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1788381, Length=182, Percent_Identity=27.4725274725275, Blast_Score=76, Evalue=7e-15,
Organism=Escherichia coli, GI1787541, Length=197, Percent_Identity=28.9340101522843, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 60149; Mature: 60018

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS50885 HAMP ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARSQSIASRIRFFSFLLVTLPLCVSLLTFAVVSRILVVGGAHHDMVVELQHHKLTTERW
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHCHHHHH
LNEQLQSITFFAHTGISDLHDTAALQQALRVFLNYHTSFSQVFYASPDGSLLTGPELPVP
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHEEEEECCCCCEEECCCCCEE
INVGDRGYFQKARQGLPVVSPVLVSRFSHNPSLIFCAPLPFADGRFAGAVIGVTDLRTLD
ECCCCCHHHHHHHCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEEEHHHHHHHH
EALLRLHRRQNDQSFLATADGYLLTSRLQENTIILPRYNPEGIPVTRTVLPPEDSLSTPH
HHHHHHHHCCCCCCEEEECCCHHEEHHCCCCEEEEECCCCCCCCEEEEECCCCCCCCCCC
FYRNTQGVYVLGVRLAFKSGQWLLVQERPVRAMLGGYSWIIAMTMAGALATAALLIPFLL
CEECCCCEEEEEEEEEECCCCEEEEECCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
KAGRRITGPIEEISTMSEELTHGHYDKECPYLHTADMPPEIRRLYDNFCFMAERVSQQMR
HCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHH
DLEHLSFCDTLTGLHNRRHLAHEGTRTITACTEADLPCSCLMLDIDFFKQVNDAWGHDAG
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEHHHHHHHHHHCCCCHH
DMVLQSVARTISNAVRSKDMTIRMGGEEFAVIAPATDTASAFSLAERIRRNIESTPVQAA
HHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCC
DDEIRITISIGVADMGGAPIFGTTQLDDLLAKADRALYQAKKKGRNRTEIWSEKLIAAAP
CCCEEEEEEEEEECCCCCCEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHEEECC
DG
CC
>Mature Secondary Structure 
ARSQSIASRIRFFSFLLVTLPLCVSLLTFAVVSRILVVGGAHHDMVVELQHHKLTTERW
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHCHHHHH
LNEQLQSITFFAHTGISDLHDTAALQQALRVFLNYHTSFSQVFYASPDGSLLTGPELPVP
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHEEEEECCCCCEEECCCCCEE
INVGDRGYFQKARQGLPVVSPVLVSRFSHNPSLIFCAPLPFADGRFAGAVIGVTDLRTLD
ECCCCCHHHHHHHCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEEEHHHHHHHH
EALLRLHRRQNDQSFLATADGYLLTSRLQENTIILPRYNPEGIPVTRTVLPPEDSLSTPH
HHHHHHHHCCCCCCEEEECCCHHEEHHCCCCEEEEECCCCCCCCEEEEECCCCCCCCCCC
FYRNTQGVYVLGVRLAFKSGQWLLVQERPVRAMLGGYSWIIAMTMAGALATAALLIPFLL
CEECCCCEEEEEEEEEECCCCEEEEECCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
KAGRRITGPIEEISTMSEELTHGHYDKECPYLHTADMPPEIRRLYDNFCFMAERVSQQMR
HCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHH
DLEHLSFCDTLTGLHNRRHLAHEGTRTITACTEADLPCSCLMLDIDFFKQVNDAWGHDAG
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEHHHHHHHHHHCCCCHH
DMVLQSVARTISNAVRSKDMTIRMGGEEFAVIAPATDTASAFSLAERIRRNIESTPVQAA
HHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCC
DDEIRITISIGVADMGGAPIFGTTQLDDLLAKADRALYQAKKKGRNRTEIWSEKLIAAAP
CCCEEEEEEEEEECCCCCCEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHEEECC
DG
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]