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The map label for this gene is cofH [H]

Identifier: 78355845

GI number: 78355845

Start: 817797

End: 818912

Strand: Reverse

Name: cofH [H]

Synonym: Dde_0798

Alternate gene names: 78355845

Gene position: 818912-817797 (Counterclockwise)

Preceding gene: 78355847

Following gene: 78355844

Centisome position: 21.95

GC content: 59.32

Gene sequence:

>1116_bases
ATGTTGAATGCCGACAGATACGCCATGCTGGGCCTTGGCGAGATTTTTGATAAAGTCACGGCGGGTGAGCGCCTGTCTCT
TGAAGACGGTGAACTGCTGTACAACTGTCCCGACATCACCGCGGTGGGGGCACTGGCCCACCACGTGCGCACCCGCATGC
ACGGAGACAGAACCTATTATGTCCTCAACAGGCAAGTCAATTACACAAACATATGTGTGAACGGCTGCCTGTTCTGCGCT
TTTCAGCGCGAACGCGGCCAGCAGGGGGCATATGTCATGCAGCCCTCGGATATCGTTGACCGCGTGCTGGACGACAAAGG
CACCCCCTTCACGGAAGTGCACATCGTGGGCGGCTGTCACCCCGACCTGCCGCTGGAATGGTTTGAAGACGTCATACGCC
GCATCAAGCAGCAGCGTCCCGAAATCGTGGTCAAGGCCTTTACCGCAGTGGAAATTGCCCACTTTGCCACACTGGCGGGC
ATCGGCACGGCAGAAGTGCTTGCCCGGCTGAAAGCCGCAGGGCTGGAATCGATGCCAGGCGGCGGCGCGGAAATTTTCGC
CCCCCGTGTGCGTGAACGCATCTGCGCGCGCAAAGCCACCGCGCAGGAGTGGCTGCGCATTGCCGGCGAAGCCCACGGGC
TGGGCATCACCACCAACTGCACCATGCTTTTCGGGCACATCGAATCCGTTGACGACAGGCTGGACCATCTGGACAGGCTG
CGCCGTCAGCAGGACGTATCCGGTGGTTTCAACTGCTTCATTCCTCTGCCGTTCCTGACAGAAAACAGTCTGCTTAAACT
GCCTGAAGACCGCAGGGGCGAACATGTGGGCCTGGACAGACTGCGTACCATCGCCGTCAGCAGACTGCTGCTGGACAACA
TACCCCACATCAAAGCCTACTGGGTCATGATGGGCATCAAAATGGCCCAGACCGCCCTGTATTTCGGTGCGGACGATCTG
GACGGCACCATAGTGGAAGAACGCATCGGTCAGGAAGCCGGTGCATCTTCGGGTCAGGGGCTTACCATCAGCAGCCTGCG
GCACATGATCACGGCATCGGGCTTTACCCCCGTGCAGCGCGATACGCATTTCAATCCTGTGGAGGCCACGCTATGA

Upstream 100 bases:

>100_bases
CACACGGTTGACACCGCGCCGCTGCAGGCCGATACTCACCGGCCTGTGCCCGTGCGGCTTCCGGCCCTTTTCACGCACCG
GCGCAAGGACGGAGGAAACC

Downstream 100 bases:

>100_bases
ACGCCACTCTGACCCCGTTTGACGAACCGCGCCAAGTGCTGGACATCTGCGCCAAAGTGCTGGACGGCAGCCGCATAAAC
GAAGCCGAAGCGGCAACCCT

Product: hypothetical protein

Products: NA

Alternate protein names: 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase subunit 2 [H]

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MLNADRYAMLGLGEIFDKVTAGERLSLEDGELLYNCPDITAVGALAHHVRTRMHGDRTYYVLNRQVNYTNICVNGCLFCA
FQRERGQQGAYVMQPSDIVDRVLDDKGTPFTEVHIVGGCHPDLPLEWFEDVIRRIKQQRPEIVVKAFTAVEIAHFATLAG
IGTAEVLARLKAAGLESMPGGGAEIFAPRVRERICARKATAQEWLRIAGEAHGLGITTNCTMLFGHIESVDDRLDHLDRL
RRQQDVSGGFNCFIPLPFLTENSLLKLPEDRRGEHVGLDRLRTIAVSRLLLDNIPHIKAYWVMMGIKMAQTALYFGADDL
DGTIVEERIGQEAGASSGQGLTISSLRHMITASGFTPVQRDTHFNPVEATL

Sequences:

>Translated_371_residues
MLNADRYAMLGLGEIFDKVTAGERLSLEDGELLYNCPDITAVGALAHHVRTRMHGDRTYYVLNRQVNYTNICVNGCLFCA
FQRERGQQGAYVMQPSDIVDRVLDDKGTPFTEVHIVGGCHPDLPLEWFEDVIRRIKQQRPEIVVKAFTAVEIAHFATLAG
IGTAEVLARLKAAGLESMPGGGAEIFAPRVRERICARKATAQEWLRIAGEAHGLGITTNCTMLFGHIESVDDRLDHLDRL
RRQQDVSGGFNCFIPLPFLTENSLLKLPEDRRGEHVGLDRLRTIAVSRLLLDNIPHIKAYWVMMGIKMAQTALYFGADDL
DGTIVEERIGQEAGASSGQGLTISSLRHMITASGFTPVQRDTHFNPVEATL
>Mature_371_residues
MLNADRYAMLGLGEIFDKVTAGERLSLEDGELLYNCPDITAVGALAHHVRTRMHGDRTYYVLNRQVNYTNICVNGCLFCA
FQRERGQQGAYVMQPSDIVDRVLDDKGTPFTEVHIVGGCHPDLPLEWFEDVIRRIKQQRPEIVVKAFTAVEIAHFATLAG
IGTAEVLARLKAAGLESMPGGGAEIFAPRVRERICARKATAQEWLRIAGEAHGLGITTNCTMLFGHIESVDDRLDHLDRL
RRQQDVSGGFNCFIPLPFLTENSLLKLPEDRRGEHVGLDRLRTIAVSRLLLDNIPHIKAYWVMMGIKMAQTALYFGADDL
DGTIVEERIGQEAGASSGQGLTISSLRHMITASGFTPVQRDTHFNPVEATL

Specific function: Catalyzes the radical-mediated transfer of the hydroxybenzyl group from 4-hydroxyphenylpyruvate (HPP) to 5-amino- 6-ribitylamino-2,4(1H,3H)-pyrimidinedione to form 7,8-didemethyl- 8-hydroxy-5-deazariboflavin (FO) [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. CofH family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR019940
- InterPro:   IPR006638
- InterPro:   IPR020050
- InterPro:   IPR005244
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 41154; Mature: 41154

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNADRYAMLGLGEIFDKVTAGERLSLEDGELLYNCPDITAVGALAHHVRTRMHGDRTYY
CCCCCHHHHHHHHHHHHHHCCCCEEECCCCCEEEECCCHHHHHHHHHHHHHHHCCCEEEE
VLNRQVNYTNICVNGCLFCAFQRERGQQGAYVMQPSDIVDRVLDDKGTPFTEVHIVGGCH
EEECCCCHHHHHHHHHHHEEEHHHCCCCCCEEECHHHHHHHHHCCCCCCEEEEEEEECCC
PDLPLEWFEDVIRRIKQQRPEIVVKAFTAVEIAHFATLAGIGTAEVLARLKAAGLESMPG
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCC
GGAEIFAPRVRERICARKATAQEWLRIAGEAHGLGITTNCTMLFGHIESVDDRLDHLDRL
CCHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHH
RRQQDVSGGFNCFIPLPFLTENSLLKLPEDRRGEHVGLDRLRTIAVSRLLLDNIPHIKAY
HHHHHCCCCCEEEEECCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHH
WVMMGIKMAQTALYFGADDLDGTIVEERIGQEAGASSGQGLTISSLRHMITASGFTPVQR
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHCHHCCCCCCCCCCHHHHHHHHHHCCCCCCCC
DTHFNPVEATL
CCCCCCCCCCC
>Mature Secondary Structure
MLNADRYAMLGLGEIFDKVTAGERLSLEDGELLYNCPDITAVGALAHHVRTRMHGDRTYY
CCCCCHHHHHHHHHHHHHHCCCCEEECCCCCEEEECCCHHHHHHHHHHHHHHHCCCEEEE
VLNRQVNYTNICVNGCLFCAFQRERGQQGAYVMQPSDIVDRVLDDKGTPFTEVHIVGGCH
EEECCCCHHHHHHHHHHHEEEHHHCCCCCCEEECHHHHHHHHHCCCCCCEEEEEEEECCC
PDLPLEWFEDVIRRIKQQRPEIVVKAFTAVEIAHFATLAGIGTAEVLARLKAAGLESMPG
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCC
GGAEIFAPRVRERICARKATAQEWLRIAGEAHGLGITTNCTMLFGHIESVDDRLDHLDRL
CCHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHH
RRQQDVSGGFNCFIPLPFLTENSLLKLPEDRRGEHVGLDRLRTIAVSRLLLDNIPHIKAY
HHHHHCCCCCEEEEECCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHH
WVMMGIKMAQTALYFGADDLDGTIVEERIGQEAGASSGQGLTISSLRHMITASGFTPVQR
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHCHHCCCCCCCCCCHHHHHHHHHHCCCCCCCC
DTHFNPVEATL
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014 [H]