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The map label for this gene is nodT [H]

Identifier: 78355447

GI number: 78355447

Start: 393342

End: 394826

Strand: Reverse

Name: nodT [H]

Synonym: Dde_0400

Alternate gene names: 78355447

Gene position: 394826-393342 (Counterclockwise)

Preceding gene: 78355448

Following gene: 78355441

Centisome position: 10.58

GC content: 63.64

Gene sequence:

>1485_bases
ATGACGCATAACAAGGCATACGCCACGCCGCGCAGAAACGGTCTGCTGGCCTCCGCTCTGCTGACCATAGTGTTTGCAGG
CGGCTGCGCCGTGGGGCCGGATTATACCGCACCGGAACTGGATTCGCCCGCCGCATGGCGCACGGAGGACGTTTCCGGCC
TGCGGCAGACCAGAAACGCCACCACCGCGCTGTCGCAGTGGTGGAAAGTGCTGGAAGATCCCATGCTGGACAGCCTGATG
CAGCGCGCCGTGACCGGCAACCTTGACCTGAAGGAAGCCCGGGCCCGCGTCCGCGCCGCACGGGCCCGTCTGGGCATCAG
CGAGGCGGACCTGTACCCCACGCTGGATGCCTCGGGCAGCCTGTCCACCGGCCAACAGTCTGAAAACGCGGGATACGACG
GCAAGGAACGTGACCTGTACAAAGCCGGATTTGATGCCGGATGGGAAATAGACATCTTCGGCGGCACGCGCCGTGCCGTT
GAAGCCGCCGGAGCCGAACTGGAAGCCAGCGAAGCCGACCTGCGCGATGTATACGTATCCATGCTGGCGGAAATAGCATC
CACCTACGTGGACGTGCGCACCTACCAGACCCGGCTTTCCGTGGCACAGGCCAACCTTGAAGAACAGAAAGAAACCTATG
AAATCGTGGCCTCGCGCTACGATTCCGGCCTGAGCGATGCACTGGGTGTGCAGCAGGCGCGCTACAGCCTTGAAAGCACC
CGCGCCGAGATTCCCGCACTGCAGTCCAAGCTGGATGCCGCCATCAACCGGCTGGCATTGCTGCTGGGGCAGCAGCCCGA
CACGCTGCATGCCGAGCTTATCACCCGGCGCCCCATACCCATGCCACCGGTCAGTGTGGCCGTGGGCATACCTGCAGACG
CGCTGCGCAACCGGCCCGACATCCGCATGGCGGAACGCACGCTGGCAGCCAGAACCGCTGAAGTGGGGGTGGCCACGGCT
GAACTGTACCCCAAATTCCGGCTGGTCGGCTCCATCGGGCTGGAATCGTTTGCCCAGAGCAGCCTGTTTGAAAGCGGCAG
TCAGGTCTACAGTTTCGGGCCGGGCATCAGCTGGCCCATTTTCCGCGCCGGAGCCATACGCCGTAATATAGATGTGGCTG
AAGCCGTGCAGGAACAGTACCTGCACCGTTACCAGCACACGGTGCTGGCCGCGCTGAATGAAGCCCAGACGGCCATGACG
GACTACGCCAAGGAACAGAACCGCCGTGACGCGCTGCAGGCAGCCACACTGGCAGCCCGCGAAGCGCTTGAACTGGCGCA
GGACAAGTACCTGAACGGCCTTGTGGACTTCAGCGACGTGCTGGAAGCCCGCCGGTCGCTGCTCTCGTACGAAGACCAGC
TGGCAGTAAGCAAGGGCGCCGTGGTGGACAGCCTGGTACGCATCTACAAATCCATGGGCGGCGGATGGCAGACCATGGAA
GCACTGCAGCAGACGGCGCAGGACGCCGGAAGCAGTCAGCGCTGA

Upstream 100 bases:

>100_bases
CTGTATGCCGCCTTTGAAACCATGCGCACCAGAACCATGGACTGGGTACACCGCAGGCGCGACGCCGCGCACGCAGAACT
GACCGATACGGGGAGCAACA

Downstream 100 bases:

>100_bases
CCGTTGCAGGCAGTCTCAGACGCACAGACACAGAAAGGGCCGGAATATTCCGGCCCTTTTTCTGTCATGCACCCCGCAAC
GGCATCCGGCCGCACAGCGT

Product: RND efflux system, outer membrane lipoprotein, NodT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 494; Mature: 493

Protein sequence:

>494_residues
MTHNKAYATPRRNGLLASALLTIVFAGGCAVGPDYTAPELDSPAAWRTEDVSGLRQTRNATTALSQWWKVLEDPMLDSLM
QRAVTGNLDLKEARARVRAARARLGISEADLYPTLDASGSLSTGQQSENAGYDGKERDLYKAGFDAGWEIDIFGGTRRAV
EAAGAELEASEADLRDVYVSMLAEIASTYVDVRTYQTRLSVAQANLEEQKETYEIVASRYDSGLSDALGVQQARYSLEST
RAEIPALQSKLDAAINRLALLLGQQPDTLHAELITRRPIPMPPVSVAVGIPADALRNRPDIRMAERTLAARTAEVGVATA
ELYPKFRLVGSIGLESFAQSSLFESGSQVYSFGPGISWPIFRAGAIRRNIDVAEAVQEQYLHRYQHTVLAALNEAQTAMT
DYAKEQNRRDALQAATLAAREALELAQDKYLNGLVDFSDVLEARRSLLSYEDQLAVSKGAVVDSLVRIYKSMGGGWQTME
ALQQTAQDAGSSQR

Sequences:

>Translated_494_residues
MTHNKAYATPRRNGLLASALLTIVFAGGCAVGPDYTAPELDSPAAWRTEDVSGLRQTRNATTALSQWWKVLEDPMLDSLM
QRAVTGNLDLKEARARVRAARARLGISEADLYPTLDASGSLSTGQQSENAGYDGKERDLYKAGFDAGWEIDIFGGTRRAV
EAAGAELEASEADLRDVYVSMLAEIASTYVDVRTYQTRLSVAQANLEEQKETYEIVASRYDSGLSDALGVQQARYSLEST
RAEIPALQSKLDAAINRLALLLGQQPDTLHAELITRRPIPMPPVSVAVGIPADALRNRPDIRMAERTLAARTAEVGVATA
ELYPKFRLVGSIGLESFAQSSLFESGSQVYSFGPGISWPIFRAGAIRRNIDVAEAVQEQYLHRYQHTVLAALNEAQTAMT
DYAKEQNRRDALQAATLAAREALELAQDKYLNGLVDFSDVLEARRSLLSYEDQLAVSKGAVVDSLVRIYKSMGGGWQTME
ALQQTAQDAGSSQR
>Mature_493_residues
THNKAYATPRRNGLLASALLTIVFAGGCAVGPDYTAPELDSPAAWRTEDVSGLRQTRNATTALSQWWKVLEDPMLDSLMQ
RAVTGNLDLKEARARVRAARARLGISEADLYPTLDASGSLSTGQQSENAGYDGKERDLYKAGFDAGWEIDIFGGTRRAVE
AAGAELEASEADLRDVYVSMLAEIASTYVDVRTYQTRLSVAQANLEEQKETYEIVASRYDSGLSDALGVQQARYSLESTR
AEIPALQSKLDAAINRLALLLGQQPDTLHAELITRRPIPMPPVSVAVGIPADALRNRPDIRMAERTLAARTAEVGVATAE
LYPKFRLVGSIGLESFAQSSLFESGSQVYSFGPGISWPIFRAGAIRRNIDVAEAVQEQYLHRYQHTVLAALNEAQTAMTD
YAKEQNRRDALQAATLAAREALELAQDKYLNGLVDFSDVLEARRSLLSYEDQLAVSKGAVVDSLVRIYKSMGGGWQTMEA
LQQTAQDAGSSQR

Specific function: In Pathogenic Strains It Allows The Bacteria To Invade Brain Microvascular Endothelial Cells (Bmec) Thus Allowing It To Cross The Blood-Brain Barrier And Cause Neonatal Meningitis. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=448, Percent_Identity=29.2410714285714, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI2367348, Length=492, Percent_Identity=26.8292682926829, Blast_Score=129, Evalue=4e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 53875; Mature: 53744

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHNKAYATPRRNGLLASALLTIVFAGGCAVGPDYTAPELDSPAAWRTEDVSGLRQTRNA
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
TTALSQWWKVLEDPMLDSLMQRAVTGNLDLKEARARVRAARARLGISEADLYPTLDASGS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCC
LSTGQQSENAGYDGKERDLYKAGFDAGWEIDIFGGTRRAVEAAGAELEASEADLRDVYVS
CCCCCCCCCCCCCCCCCHHHHHCCCCCCEEEEECCCHHHHHHCCCCCCCHHHHHHHHHHH
MLAEIASTYVDVRTYQTRLSVAQANLEEQKETYEIVASRYDSGLSDALGVQQARYSLEST
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
RAEIPALQSKLDAAINRLALLLGQQPDTLHAELITRRPIPMPPVSVAVGIPADALRNRPD
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCEEECCCHHHHCCCCC
IRMAERTLAARTAEVGVATAELYPKFRLVGSIGLESFAQSSLFESGSQVYSFGPGISWPI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHH
FRAGAIRRNIDVAEAVQEQYLHRYQHTVLAALNEAQTAMTDYAKEQNRRDALQAATLAAR
HHHCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EALELAQDKYLNGLVDFSDVLEARRSLLSYEDQLAVSKGAVVDSLVRIYKSMGGGWQTME
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
ALQQTAQDAGSSQR
HHHHHHHHCCCCCC
>Mature Secondary Structure 
THNKAYATPRRNGLLASALLTIVFAGGCAVGPDYTAPELDSPAAWRTEDVSGLRQTRNA
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
TTALSQWWKVLEDPMLDSLMQRAVTGNLDLKEARARVRAARARLGISEADLYPTLDASGS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCC
LSTGQQSENAGYDGKERDLYKAGFDAGWEIDIFGGTRRAVEAAGAELEASEADLRDVYVS
CCCCCCCCCCCCCCCCCHHHHHCCCCCCEEEEECCCHHHHHHCCCCCCCHHHHHHHHHHH
MLAEIASTYVDVRTYQTRLSVAQANLEEQKETYEIVASRYDSGLSDALGVQQARYSLEST
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
RAEIPALQSKLDAAINRLALLLGQQPDTLHAELITRRPIPMPPVSVAVGIPADALRNRPD
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCEEECCCHHHHCCCCC
IRMAERTLAARTAEVGVATAELYPKFRLVGSIGLESFAQSSLFESGSQVYSFGPGISWPI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHH
FRAGAIRRNIDVAEAVQEQYLHRYQHTVLAALNEAQTAMTDYAKEQNRRDALQAATLAAR
HHHCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EALELAQDKYLNGLVDFSDVLEARRSLLSYEDQLAVSKGAVVDSLVRIYKSMGGGWQTME
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
ALQQTAQDAGSSQR
HHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2338917; 2622339 [H]