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The map label for this gene is nirB [C]

Identifier: 78355421

GI number: 78355421

Start: 367903

End: 369609

Strand: Reverse

Name: nirB [C]

Synonym: Dde_0374

Alternate gene names: 78355421

Gene position: 369609-367903 (Counterclockwise)

Preceding gene: 78355422

Following gene: 78355420

Centisome position: 9.91

GC content: 60.05

Gene sequence:

>1707_bases
ATGCCCCAGAATATCGTCGTTATCGGCGCCGTTGCGCTAGGTCCCAAAGCCGCATGCCGTTTCAAGCGGCTGTGCCCCGA
AGACAACGTCATCATGATCGACCAGTCCGACCGCATCTCTTACGGCGGCTGCGGTATTCCCTACTATGTTTCGGGCGAGG
TCAACACGCTTGAAGAGCTGCAGATGACCCCCTACCACACCATCCGCGACGCCGAATTTTTCCGGGTGAACAAAGACGTC
AAGGTGCTGGCCCGCACCAAGGTCGAATCCATCGACCGGCAGGCCAAAAAAGTACTCATCAGAGACCTGACCACAGGTGA
GACAGCAGAACTGCCCTACGACAAGCTGGTGCTGGCCATGGGCAGCGCACCCAACCGTCCCCCGTTTGAAGGCATTGACC
TTGAAGGCGTGCACACCTGCACCACGCTGGACGACGCGCAGGCCATCCGCGAAGCCGTGGCCGCCGGTTCCGTAAGCGGT
GCCGTGGTTGTGGGCGGCGGCTTCATAGGCCTTGAAGTGGCTGTCTCGCTGGCCGACATGTGGGGCATCAAAACATCTGT
GGTGGAAATAGCAGACCAGATTCTGCCCGGTTTTCTGAGCGGCAACATGGTCAAGATTGCCACACACGATCTGCGCAAAA
ATGACGTGGACGTCTTCTGCGGCGAAAAAGTCATCCGCATCGAAGGCGAAAACGGCAAGGTTGCCCGTGTGGTCACCGAC
AAACGCACCATTGACGCCGAACTGGTAGTCATGGCGGCAGGCATCCGACCCAACACCGAAATTGCCCGTGCAGCCGGTAT
CGAGCTGAACGAACGCGGCGCCATTATCGTCAATGACCGCATGCAGACGTCCGACCCCGACATCTATGCCGGCGGCGACT
GCGTAACCGTGCCCAATCTGGTTACCGGCAAACCCGGCTTCTTCCCTCTCGGCTCCATGGCCAACAGACAGGGACGCGTC
ATCGGCACCAACCTTGCCGGCGGCGATGCCCGGTTCCCCGGCGGCGTGGGCACCTGGGCCGTCAAACTGTTTGAACAGAA
CGCGGCCGGTGCGGGTCTGACCATCGAGTCGGCCCTGCGCGAAGGATTTGATGCGGTCAACGTGCACGTGGAAGGCTTCG
ACCGCGCTCACTTCTACCCCGAACACACCGTCATGGCACTGGATCTGGTTGTGGAACGCCCCACCCGCCGCGTACTGGGC
ATTCAGGGCACCAGCAGTCTGGGCGACGCGCTTGTGGCCCGCATCAATGCCGTGGCGCCGCTGCTGCAGAACAAACCCAA
GCTGGAAGACATATCCAACCTTGAAGTGGCTTACTCGCCGCCTTTCGCCTCTGCCATGGACATCATCAACACCGTGGCCA
ACGTGGCGGAAAACGTGCTTGAGGGCCGCAACCACACGCTGGACCCCGATGAATTCGCCGTCATGTGGCAGAACCGCGAG
TCGGGCAGCTGCACCTTTGTGGACACGCGTCTGGCCTCCAACGCTGAATCGCTGGTGGAAAAATACCCCGGTCAGTGGCT
TTCCATTCCGCTGGAAGAAATGGAGCAGCGCCTCGACGAAATACCCGAAGACAAGACCGTGGTGCTTATCTGCAACACCG
GTCTGCGTTCCTTTGAATCGCAGCTGATCATGAACCGTCACGGCCGCAAAAGCCTGAGCGTTCAGGGCGGCATGGCTTCG
GTGACCAAGCAGGGCAACGAAATCTGA

Upstream 100 bases:

>100_bases
CGCAAGGATACAGGCGACAGACGGTATGCGGTCATCATGCCGCGGCAGCAGGATTCCTGTTGCGCCGCTGCCGTTCAATT
CACGCAGACCGGAGGAATTC

Downstream 100 bases:

>100_bases
TCCGCCCATCCGGCATCACCGCGGCGCGGCCATACGGCCGCGCCGCTTTTTTTCATGCCTTCCGCCGCTTGCCTGTACAA
AAGCATACGGCTACAGTGCG

Product: rhodanese-like protein

Products: NA

Alternate protein names: CoA-disulfide reductase; CoADR [H]

Number of amino acids: Translated: 568; Mature: 567

Protein sequence:

>568_residues
MPQNIVVIGAVALGPKAACRFKRLCPEDNVIMIDQSDRISYGGCGIPYYVSGEVNTLEELQMTPYHTIRDAEFFRVNKDV
KVLARTKVESIDRQAKKVLIRDLTTGETAELPYDKLVLAMGSAPNRPPFEGIDLEGVHTCTTLDDAQAIREAVAAGSVSG
AVVVGGGFIGLEVAVSLADMWGIKTSVVEIADQILPGFLSGNMVKIATHDLRKNDVDVFCGEKVIRIEGENGKVARVVTD
KRTIDAELVVMAAGIRPNTEIARAAGIELNERGAIIVNDRMQTSDPDIYAGGDCVTVPNLVTGKPGFFPLGSMANRQGRV
IGTNLAGGDARFPGGVGTWAVKLFEQNAAGAGLTIESALREGFDAVNVHVEGFDRAHFYPEHTVMALDLVVERPTRRVLG
IQGTSSLGDALVARINAVAPLLQNKPKLEDISNLEVAYSPPFASAMDIINTVANVAENVLEGRNHTLDPDEFAVMWQNRE
SGSCTFVDTRLASNAESLVEKYPGQWLSIPLEEMEQRLDEIPEDKTVVLICNTGLRSFESQLIMNRHGRKSLSVQGGMAS
VTKQGNEI

Sequences:

>Translated_568_residues
MPQNIVVIGAVALGPKAACRFKRLCPEDNVIMIDQSDRISYGGCGIPYYVSGEVNTLEELQMTPYHTIRDAEFFRVNKDV
KVLARTKVESIDRQAKKVLIRDLTTGETAELPYDKLVLAMGSAPNRPPFEGIDLEGVHTCTTLDDAQAIREAVAAGSVSG
AVVVGGGFIGLEVAVSLADMWGIKTSVVEIADQILPGFLSGNMVKIATHDLRKNDVDVFCGEKVIRIEGENGKVARVVTD
KRTIDAELVVMAAGIRPNTEIARAAGIELNERGAIIVNDRMQTSDPDIYAGGDCVTVPNLVTGKPGFFPLGSMANRQGRV
IGTNLAGGDARFPGGVGTWAVKLFEQNAAGAGLTIESALREGFDAVNVHVEGFDRAHFYPEHTVMALDLVVERPTRRVLG
IQGTSSLGDALVARINAVAPLLQNKPKLEDISNLEVAYSPPFASAMDIINTVANVAENVLEGRNHTLDPDEFAVMWQNRE
SGSCTFVDTRLASNAESLVEKYPGQWLSIPLEEMEQRLDEIPEDKTVVLICNTGLRSFESQLIMNRHGRKSLSVQGGMAS
VTKQGNEI
>Mature_567_residues
PQNIVVIGAVALGPKAACRFKRLCPEDNVIMIDQSDRISYGGCGIPYYVSGEVNTLEELQMTPYHTIRDAEFFRVNKDVK
VLARTKVESIDRQAKKVLIRDLTTGETAELPYDKLVLAMGSAPNRPPFEGIDLEGVHTCTTLDDAQAIREAVAAGSVSGA
VVVGGGFIGLEVAVSLADMWGIKTSVVEIADQILPGFLSGNMVKIATHDLRKNDVDVFCGEKVIRIEGENGKVARVVTDK
RTIDAELVVMAAGIRPNTEIARAAGIELNERGAIIVNDRMQTSDPDIYAGGDCVTVPNLVTGKPGFFPLGSMANRQGRVI
GTNLAGGDARFPGGVGTWAVKLFEQNAAGAGLTIESALREGFDAVNVHVEGFDRAHFYPEHTVMALDLVVERPTRRVLGI
QGTSSLGDALVARINAVAPLLQNKPKLEDISNLEVAYSPPFASAMDIINTVANVAENVLEGRNHTLDPDEFAVMWQNRES
GSCTFVDTRLASNAESLVEKYPGQWLSIPLEEMEQRLDEIPEDKTVVLICNTGLRSFESQLIMNRHGRKSLSVQGGMASV
TKQGNEI

Specific function: Nitrate assimilation (denitrification); second step. [C]

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=380, Percent_Identity=28.6842105263158, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI65787454, Length=380, Percent_Identity=28.6842105263158, Blast_Score=123, Evalue=5e-28,
Organism=Homo sapiens, GI226437568, Length=380, Percent_Identity=28.6842105263158, Blast_Score=123, Evalue=6e-28,
Organism=Escherichia coli, GI1789765, Length=332, Percent_Identity=27.710843373494, Blast_Score=99, Evalue=1e-21,
Organism=Escherichia coli, GI1789065, Length=220, Percent_Identity=29.0909090909091, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1787352, Length=264, Percent_Identity=28.7878787878788, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI87082354, Length=315, Percent_Identity=24.4444444444444, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1788892, Length=224, Percent_Identity=25.8928571428571, Blast_Score=62, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI17559934, Length=235, Percent_Identity=28.5106382978723, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI32564386, Length=392, Percent_Identity=23.469387755102, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24639257, Length=336, Percent_Identity=26.1904761904762, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI281359715, Length=267, Percent_Identity=28.0898876404494, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI281359713, Length=267, Percent_Identity=28.0898876404494, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24639250, Length=267, Percent_Identity=28.0898876404494, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI18543267, Length=267, Percent_Identity=28.0898876404494, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24639252, Length=267, Percent_Identity=28.0898876404494, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24585130, Length=301, Percent_Identity=26.2458471760797, Blast_Score=82, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017758
- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.14 [H]

Molecular weight: Translated: 61342; Mature: 61211

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQNIVVIGAVALGPKAACRFKRLCPEDNVIMIDQSDRISYGGCGIPYYVSGEVNTLEEL
CCCCEEEEEEEECCCHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCEEECCCCCCHHHH
QMTPYHTIRDAEFFRVNKDVKVLARTKVESIDRQAKKVLIRDLTTGETAELPYDKLVLAM
CCCCCCEECCCEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHEEEEE
GSAPNRPPFEGIDLEGVHTCTTLDDAQAIREAVAAGSVSGAVVVGGGFIGLEVAVSLADM
CCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHCCCCCCEEEEECCEEHHHHHHHHHHH
WGIKTSVVEIADQILPGFLSGNMVKIATHDLRKNDVDVFCGEKVIRIEGENGKVARVVTD
HCCHHHHHHHHHHHCCHHHCCCEEEEEECCCCCCCCEEEECCEEEEEECCCCCEEEEEEC
KRTIDAELVVMAAGIRPNTEIARAAGIELNERGAIIVNDRMQTSDPDIYAGGDCVTVPNL
CCCCCEEEEEEEECCCCCCHHHHHCCCEECCCCEEEECCCCCCCCCCEEECCCEEECCCC
VTGKPGFFPLGSMANRQGRVIGTNLAGGDARFPGGVGTWAVKLFEQNAAGAGLTIESALR
CCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCHHEEEEECCCCCCCCCCHHHHHH
EGFDAVNVHVEGFDRAHFYPEHTVMALDLVVERPTRRVLGIQGTSSLGDALVARINAVAP
CCCCEEEEEEECCCCCCCCCCCCEEEEEEHHCCCCCCEEEECCCCHHHHHHHHHHHHHHH
LLQNKPKLEDISNLEVAYSPPFASAMDIINTVANVAENVLEGRNHTLDPDEFAVMWQNRE
HHCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCC
SGSCTFVDTRLASNAESLVEKYPGQWLSIPLEEMEQRLDEIPEDKTVVLICNTGLRSFES
CCCEEEEEHHHHCCHHHHHHHCCCCEEECCHHHHHHHHHHCCCCCEEEEEECCCCHHHHH
QLIMNRHGRKSLSVQGGMASVTKQGNEI
HHHHHCCCCCEEEECCCHHHHCCCCCCC
>Mature Secondary Structure 
PQNIVVIGAVALGPKAACRFKRLCPEDNVIMIDQSDRISYGGCGIPYYVSGEVNTLEEL
CCCEEEEEEEECCCHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCEEECCCCCCHHHH
QMTPYHTIRDAEFFRVNKDVKVLARTKVESIDRQAKKVLIRDLTTGETAELPYDKLVLAM
CCCCCCEECCCEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHEEEEE
GSAPNRPPFEGIDLEGVHTCTTLDDAQAIREAVAAGSVSGAVVVGGGFIGLEVAVSLADM
CCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHCCCCCCEEEEECCEEHHHHHHHHHHH
WGIKTSVVEIADQILPGFLSGNMVKIATHDLRKNDVDVFCGEKVIRIEGENGKVARVVTD
HCCHHHHHHHHHHHCCHHHCCCEEEEEECCCCCCCCEEEECCEEEEEECCCCCEEEEEEC
KRTIDAELVVMAAGIRPNTEIARAAGIELNERGAIIVNDRMQTSDPDIYAGGDCVTVPNL
CCCCCEEEEEEEECCCCCCHHHHHCCCEECCCCEEEECCCCCCCCCCEEECCCEEECCCC
VTGKPGFFPLGSMANRQGRVIGTNLAGGDARFPGGVGTWAVKLFEQNAAGAGLTIESALR
CCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCHHEEEEECCCCCCCCCCHHHHHH
EGFDAVNVHVEGFDRAHFYPEHTVMALDLVVERPTRRVLGIQGTSSLGDALVARINAVAP
CCCCEEEEEEECCCCCCCCCCCCEEEEEEHHCCCCCCEEEECCCCHHHHHHHHHHHHHHH
LLQNKPKLEDISNLEVAYSPPFASAMDIINTVANVAENVLEGRNHTLDPDEFAVMWQNRE
HHCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCC
SGSCTFVDTRLASNAESLVEKYPGQWLSIPLEEMEQRLDEIPEDKTVVLICNTGLRSFES
CCCEEEEEHHHHCCHHHHHHHCCCCEEECCHHHHHHHHHHCCCCCEEEEEECCCCHHHHH
QLIMNRHGRKSLSVQGGMASVTKQGNEI
HHHHHCCCCCEEEECCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA