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The map label for this gene is nuoI [C]

Identifier: 78355312

GI number: 78355312

Start: 250065

End: 251348

Strand: Reverse

Name: nuoI [C]

Synonym: Dde_0265

Alternate gene names: 78355312

Gene position: 251348-250065 (Counterclockwise)

Preceding gene: 78355313

Following gene: 78355311

Centisome position: 6.74

GC content: 58.8

Gene sequence:

>1284_bases
ATGGGACATCTGGCAGCCAAAGACATCTACCGTTCACTGGCGGCAACCATAGACGGCACCCCCGTGCGCACGCCCGATTC
CCCCGCGTTTCAGGCCATGCTGCGCGCCCTGTATACCCCGCAGGAAGCAGCTCTTGTCAGCCGCATGCCGTACCGCCCCT
CCACCGCGGCCCGCATAGCCGCACTCTGCGGCATACCGCCCGAAGACCTTGTCCCGCAGCTTGAAACCCTGTGCGGCAAA
GGCCTTGTGCTCGATATCTGGGACGGCAGCACATATCAGTACATGATAAGCCCCATCGTCATCGGCATCTTCGAGTTCAC
CATGATGCGCACCGGGGGAAACCTTCCGCTCAGGCAGTGGGCAGAACTTTTCCACGACTACATGTTTGGCGACAAAGCCT
TTTTCGAAGCAAACTTCGGCGACGGTCAGCAGGTATCCATCATGCGCGCACTGCCCTATGAACAGGTGCTTGCCGGCGAC
CACGTGGAAATACTGGATTATGAAAAAGCCTCTGCGCTGGTGGAACGGCACAGTGTTTTTTCCGTGGGGCTGTGTTCCTG
CAGACACGAAAAACATCATATGGGGCAGAAATGCCGCGTGCCCATGGAAACGTGCACTTCCATGGGCAGCGGAGCGGAAT
TTCTCATCCGCAACAAGTTCGCACGCCGCATAGACAAAGCGGAAATGCTCGACATACTGGACAGGTCGCGTGATTTCGCA
CTCACACTTTCCACCGACAACGTCCGTCAGGACGCCAATTTCATCTGCCACTGCTGCGGCTGCTGCTGCAATCTGATGCA
GGGCATACGCGAAACGGGGCTGGGCAACATTCTTGTTTCATCAACCTGCATGGCGCAGATACAGGAAGAACGCTGCACCG
GCTGCGGCAAATGTGCGGCGGCGTGCCCCATAGACGCCATAACCATGGTACCCGCAGCACAGACTACCCCGGACACCCGC
AGACGCAGAAGCGTACAGGTGGACACATCGCTTTGTCTGGGCTGCGGGGCATGCGCCCTGAAATGCCCGACGGGGGCCAT
GGTGCTGGAAAAGCGCCGGCAGCGGGTCTTTCATCCCGAAGATTCATTCGAACGGGTCATCCTGCAGAGTCTTGAACGCG
GAACGCTGCAGAACCTGATATTTGACAACCCCAACAGCCGTACCGAGCACTTCATGCGGCACCTTCTGGGCGGTTTTCTG
CGCCTGTCCCCCGTCAAACGCGCTCTGATGAGCGAAACGCTGCGCTCGCGGTTTCTGACGGCATTCCGCAACGCCGCGCT
CTGA

Upstream 100 bases:

>100_bases
CCGCCGCGCACACATTGCCAAACCTGCCCGCCCGTGCCATGTACACGGCGGGCGGGCTGCCTGTGCATGCCCGCCGGCAT
AACTGCATGACGGAAAAGCA

Downstream 100 bases:

>100_bases
CACACACCGGAGAAACGCACATATGCATCAGGCACCGTTCGAGGCACCGCCTTCTTTGCCCGCATGGCATGCACGTGATG
ACAGCGGGCCCGACCTTGTG

Product: iron-sulfur cluster-binding protein

Products: NAD+; ubiquinol [C]

Alternate protein names: Ferredoxin; Pyridine Nucleotide-Disulphide Oxidoreductase; 4Fe-4S Ferredoxin; FAD-Dependent Pyridine Nucleotide-Disulfide Oxidoreductase; FAD-Dependent Pyridine Nucleotide-Disulphide Oxidoreductase

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MGHLAAKDIYRSLAATIDGTPVRTPDSPAFQAMLRALYTPQEAALVSRMPYRPSTAARIAALCGIPPEDLVPQLETLCGK
GLVLDIWDGSTYQYMISPIVIGIFEFTMMRTGGNLPLRQWAELFHDYMFGDKAFFEANFGDGQQVSIMRALPYEQVLAGD
HVEILDYEKASALVERHSVFSVGLCSCRHEKHHMGQKCRVPMETCTSMGSGAEFLIRNKFARRIDKAEMLDILDRSRDFA
LTLSTDNVRQDANFICHCCGCCCNLMQGIRETGLGNILVSSTCMAQIQEERCTGCGKCAAACPIDAITMVPAAQTTPDTR
RRRSVQVDTSLCLGCGACALKCPTGAMVLEKRRQRVFHPEDSFERVILQSLERGTLQNLIFDNPNSRTEHFMRHLLGGFL
RLSPVKRALMSETLRSRFLTAFRNAAL

Sequences:

>Translated_427_residues
MGHLAAKDIYRSLAATIDGTPVRTPDSPAFQAMLRALYTPQEAALVSRMPYRPSTAARIAALCGIPPEDLVPQLETLCGK
GLVLDIWDGSTYQYMISPIVIGIFEFTMMRTGGNLPLRQWAELFHDYMFGDKAFFEANFGDGQQVSIMRALPYEQVLAGD
HVEILDYEKASALVERHSVFSVGLCSCRHEKHHMGQKCRVPMETCTSMGSGAEFLIRNKFARRIDKAEMLDILDRSRDFA
LTLSTDNVRQDANFICHCCGCCCNLMQGIRETGLGNILVSSTCMAQIQEERCTGCGKCAAACPIDAITMVPAAQTTPDTR
RRRSVQVDTSLCLGCGACALKCPTGAMVLEKRRQRVFHPEDSFERVILQSLERGTLQNLIFDNPNSRTEHFMRHLLGGFL
RLSPVKRALMSETLRSRFLTAFRNAAL
>Mature_426_residues
GHLAAKDIYRSLAATIDGTPVRTPDSPAFQAMLRALYTPQEAALVSRMPYRPSTAARIAALCGIPPEDLVPQLETLCGKG
LVLDIWDGSTYQYMISPIVIGIFEFTMMRTGGNLPLRQWAELFHDYMFGDKAFFEANFGDGQQVSIMRALPYEQVLAGDH
VEILDYEKASALVERHSVFSVGLCSCRHEKHHMGQKCRVPMETCTSMGSGAEFLIRNKFARRIDKAEMLDILDRSRDFAL
TLSTDNVRQDANFICHCCGCCCNLMQGIRETGLGNILVSSTCMAQIQEERCTGCGKCAAACPIDAITMVPAAQTTPDTRR
RRSVQVDTSLCLGCGACALKCPTGAMVLEKRRQRVFHPEDSFERVILQSLERGTLQNLIFDNPNSRTEHFMRHLLGGFLR
LSPVKRALMSETLRSRFLTAFRNAAL

Specific function: Ndh-1 Shuttles Electrons From NADH, Via Fmn And Iron- Sulfur (Fe-S) Centers, To Quinones In The Respiratory Chain. The Immediate Electron Acceptor For The Enzyme In This Species Is Believed To Be Ubiquinone. Couples The Redox Reaction To Proton Translocat

COG id: COG1145

COG function: function code C; Ferredoxin

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 1.6.99.5 [C]

Molecular weight: Translated: 47512; Mature: 47380

Theoretical pI: Translated: 7.78; Mature: 7.78

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.9 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
9.1 %Cys+Met (Translated Protein)
4.9 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
8.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGHLAAKDIYRSLAATIDGTPVRTPDSPAFQAMLRALYTPQEAALVSRMPYRPSTAARIA
CCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHCCCCCCCHHHHHH
ALCGIPPEDLVPQLETLCGKGLVLDIWDGSTYQYMISPIVIGIFEFTMMRTGGNLPLRQW
HHHCCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
AELFHDYMFGDKAFFEANFGDGQQVSIMRALPYEQVLAGDHVEILDYEKASALVERHSVF
HHHHHHHHCCCHHEEECCCCCCCEEHHHHHCCHHHHHCCCCEEEECHHHHHHHHHHHHHH
SVGLCSCRHEKHHMGQKCRVPMETCTSMGSGAEFLIRNKFARRIDKAEMLDILDRSRDFA
HHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
LTLSTDNVRQDANFICHCCGCCCNLMQGIRETGLGNILVSSTCMAQIQEERCTGCGKCAA
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHH
ACPIDAITMVPAAQTTPDTRRRRSVQVDTSLCLGCGACALKCPTGAMVLEKRRQRVFHPE
CCCCCHHEECCCCCCCCHHHHHCCEEECHHHHHHCCCHHEECCCCHHHHHHHHHHCCCCC
DSFERVILQSLERGTLQNLIFDNPNSRTEHFMRHLLGGFLRLSPVKRALMSETLRSRFLT
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFRNAAL
HHHHCCC
>Mature Secondary Structure 
GHLAAKDIYRSLAATIDGTPVRTPDSPAFQAMLRALYTPQEAALVSRMPYRPSTAARIA
CCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHCCCCCCCHHHHHH
ALCGIPPEDLVPQLETLCGKGLVLDIWDGSTYQYMISPIVIGIFEFTMMRTGGNLPLRQW
HHHCCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
AELFHDYMFGDKAFFEANFGDGQQVSIMRALPYEQVLAGDHVEILDYEKASALVERHSVF
HHHHHHHHCCCHHEEECCCCCCCEEHHHHHCCHHHHHCCCCEEEECHHHHHHHHHHHHHH
SVGLCSCRHEKHHMGQKCRVPMETCTSMGSGAEFLIRNKFARRIDKAEMLDILDRSRDFA
HHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
LTLSTDNVRQDANFICHCCGCCCNLMQGIRETGLGNILVSSTCMAQIQEERCTGCGKCAA
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHH
ACPIDAITMVPAAQTTPDTRRRRSVQVDTSLCLGCGACALKCPTGAMVLEKRRQRVFHPE
CCCCCHHEECCCCCCCCHHHHHCCEEECHHHHHHCCCHHEECCCCHHHHHHHHHHCCCCC
DSFERVILQSLERGTLQNLIFDNPNSRTEHFMRHLLGGFLRLSPVKRALMSETLRSRFLT
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFRNAAL
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; ubiquinone [C]

Specific reaction: NADH + ubiquinone = NAD+ + ubiquinol [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA