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The map label for this gene is topA [H]

Identifier: 78355153

GI number: 78355153

Start: 95374

End: 97617

Strand: Reverse

Name: topA [H]

Synonym: Dde_0106

Alternate gene names: 78355153

Gene position: 97617-95374 (Counterclockwise)

Preceding gene: 78355156

Following gene: 78355150

Centisome position: 2.62

GC content: 57.53

Gene sequence:

>2244_bases
ATGGGCAAAGACCTCATCATCGTCGAGTCTCCCGCCAAGGTGAAGACTATCAAAAAGTTTCTGGGCAACAAATATATGGT
CCAGGCCAGTGTGGGACATATCCGCGACCTGCCGACCAAGGAGCTCGGCGTGGACGAGGAAAACGACTTTTCTCCTGACT
ATCAGGTAATTCAGGGAAAACAAAAGGTTGTTTCCGCCCTTCGCGAAGCTGCGGCAAAAGCTGATAATGTATTTCTGGCG
CCTGACCCCGACCGCGAGGGCGAGGCCATTGCCTGGCATGTTGCTGAAATAATTGCCAAGGAAAACAAAAACATCAGCCG
TATCCAGTTTAACGAGATTACCGCCCGCGCCGTGCGCGAAGCGCTGGAAAACCCGCGCGAGCTGAACCGGAGTCTGTTTG
ACGCACAACAGGCACGCCGCGTGCTCGACAGACTGGTCGGTTACAAAATTTCGCCCATTCTGTGGAACAAAATAAAACGC
GGCATCTCTGCCGGCAGGGTGCAGTCGGTGGCGCTGCGGCTGATTGTAGACCGCGAACGCGAACGCTATGCATTCACATC
GGAAGAATACTGGGTTTTCCGGGCCAATCTGGCCGCGGAAACGCCCCCGCCCTTCAAGGCGGAACTTTTCAAGGTGGATG
ACAAAAAACCCGCCATTGCCAGCGCGGAGCAGGCGCAGGAACTGGAAAAGTCCATTGAGGGCAAAGCGTTCATCGTGGAT
TCGGTGCAGGAGAAAGAACGCTCACGCAAACCGCAGCCGCCGTTCATCACCTCCACCCTGCAGCAGCTGGCCAGCCAGCG
GCTCGGTTTTTCCGCCAAGCGCACCATGTCGGTGGCGCAGCGGCTGTATGAAGGCCTTGATCTGGGCGACATGGGCACCA
CGGCGCTCATCACCTATATGCGTACCGACTCCGTCCGCATTTCGGATGAAGCCCGCGACAGCGCGCGCGAGCTTATCGCC
TCGCTGTACGGCAAAGAATACTGCCCCGCCAAGGCGCCCGTATACAAAACCAAAGGCTCGGCACAGGACGCGCACGAAGC
CATCCGCCCCGTTGACGTAACGCTGACTCCGGACAAAATAAAAGGTCTGGTGGCACCGGAACAGTACAGCCTGTACCGCC
TTATCTGGTCGCGGTTCATGGCCTCGCAGATGGCGCCTGCCCGCTTCCACGACACCACGGTGCTTATCGGATGCGGCGGC
ACGCAGTGGCGCGTAAAAGGCGAACGGCTGCTTTTTCCGGGGTATATGGCCGCCGCGCCTGCTTCGGCCAAAGAAGCCAA
TGTGGAGCTGCCCCCCATGCAGCAGGGGCAGACGCTGGAACTGACCCTGCTGGAAAAAGAGCAGAAGTTCACACAGCCTC
CCGCCCGCTATTCCGAAGCCTCTCTGGTGCGGGAGATGGAAGAAAAAGGCATCGGCCGTCCCTCAACGTATGCCAGCATC
ATTTCCACCATTGTAGACCGCGACTACACCCGTCTGGAAGAGAAGCACTTTGTGCCCACCGATCTCGGCTTTGTGGTGTG
TGACATGCTTACCAACCACTTCGGCACGCTGATGGATATCGGCTTTACCGCGCAGATGGAAAATTCGCTGGATAAAGTGG
CCGAAGGCGACCTTGACTGGGTGGCACTGATGAAGGACTTCACCAGCGACTTCAACCCTACGCTGGCATCCGCCGCCAAA
GAAATGAAAACCGTGAAGGCGGGGCTGGAAACGGACATCGTGTGCTCTGAATGCGGCAAACCCATGGCCATCAAGTTCGG
CAAGGCCGGCCCCTTTCTGGCATGCACCGGCTACCCCGACTGCAAAAACACCAGTAACTTCACACGTGATGAAAGCGGAA
CAATACAGCTGGTTGAACGTGAGCAGGAAGAACTGAAACCCGTCGGCACCTGCCCCGAATGCGGCAAAGACCTTGTGGTC
AAACAGGCACGCACGGGCAGCAGATTCATCGCCTGTACGGGCTATCCCGACTGCAAACATACCGAACCGTTTTCCACCGG
CGTTCCCTGCCCCAATGACGGCTGCGACGGCCAGCTGGTGGAAAAAAGCTCGCGCCGCGGCAAGATATTCTATTCCTGCA
ACCGGTACCCCCAGTGCGACTATGCCACATGGAACTGGCCTGTAGCCGAAGCCTGCCCGCAGTGCGATTCCAAAATTCTG
ACTATCAAGTCCACCAAGGCAAGGGGCAAACATATTGCCTGCCCTGCCTGCAAGTACACGCGGGCGCTGGAAGACGAAGA
ATAG

Upstream 100 bases:

>100_bases
GCATGTGACAGCCCGACGGCCGCACAGGCTTTATACACCAAGCACCCGAAAGGCCGCAACGCGCTCCGTCCGCGGAACCG
CCTGCGAACAAGGACAACTC

Downstream 100 bases:

>100_bases
CATGACGCGGGGCTTAACGGCCCCGCGTTTTTTTGTGGATTTATGCTAGGGGCGGCTTCAGCCCGTCAGATATTCTGCAA
ACGGTATGCGGCGTTTTTGT

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 747; Mature: 746

Protein sequence:

>747_residues
MGKDLIIVESPAKVKTIKKFLGNKYMVQASVGHIRDLPTKELGVDEENDFSPDYQVIQGKQKVVSALREAAAKADNVFLA
PDPDREGEAIAWHVAEIIAKENKNISRIQFNEITARAVREALENPRELNRSLFDAQQARRVLDRLVGYKISPILWNKIKR
GISAGRVQSVALRLIVDRERERYAFTSEEYWVFRANLAAETPPPFKAELFKVDDKKPAIASAEQAQELEKSIEGKAFIVD
SVQEKERSRKPQPPFITSTLQQLASQRLGFSAKRTMSVAQRLYEGLDLGDMGTTALITYMRTDSVRISDEARDSARELIA
SLYGKEYCPAKAPVYKTKGSAQDAHEAIRPVDVTLTPDKIKGLVAPEQYSLYRLIWSRFMASQMAPARFHDTTVLIGCGG
TQWRVKGERLLFPGYMAAAPASAKEANVELPPMQQGQTLELTLLEKEQKFTQPPARYSEASLVREMEEKGIGRPSTYASI
ISTIVDRDYTRLEEKHFVPTDLGFVVCDMLTNHFGTLMDIGFTAQMENSLDKVAEGDLDWVALMKDFTSDFNPTLASAAK
EMKTVKAGLETDIVCSECGKPMAIKFGKAGPFLACTGYPDCKNTSNFTRDESGTIQLVEREQEELKPVGTCPECGKDLVV
KQARTGSRFIACTGYPDCKHTEPFSTGVPCPNDGCDGQLVEKSSRRGKIFYSCNRYPQCDYATWNWPVAEACPQCDSKIL
TIKSTKARGKHIACPACKYTRALEDEE

Sequences:

>Translated_747_residues
MGKDLIIVESPAKVKTIKKFLGNKYMVQASVGHIRDLPTKELGVDEENDFSPDYQVIQGKQKVVSALREAAAKADNVFLA
PDPDREGEAIAWHVAEIIAKENKNISRIQFNEITARAVREALENPRELNRSLFDAQQARRVLDRLVGYKISPILWNKIKR
GISAGRVQSVALRLIVDRERERYAFTSEEYWVFRANLAAETPPPFKAELFKVDDKKPAIASAEQAQELEKSIEGKAFIVD
SVQEKERSRKPQPPFITSTLQQLASQRLGFSAKRTMSVAQRLYEGLDLGDMGTTALITYMRTDSVRISDEARDSARELIA
SLYGKEYCPAKAPVYKTKGSAQDAHEAIRPVDVTLTPDKIKGLVAPEQYSLYRLIWSRFMASQMAPARFHDTTVLIGCGG
TQWRVKGERLLFPGYMAAAPASAKEANVELPPMQQGQTLELTLLEKEQKFTQPPARYSEASLVREMEEKGIGRPSTYASI
ISTIVDRDYTRLEEKHFVPTDLGFVVCDMLTNHFGTLMDIGFTAQMENSLDKVAEGDLDWVALMKDFTSDFNPTLASAAK
EMKTVKAGLETDIVCSECGKPMAIKFGKAGPFLACTGYPDCKNTSNFTRDESGTIQLVEREQEELKPVGTCPECGKDLVV
KQARTGSRFIACTGYPDCKHTEPFSTGVPCPNDGCDGQLVEKSSRRGKIFYSCNRYPQCDYATWNWPVAEACPQCDSKIL
TIKSTKARGKHIACPACKYTRALEDEE
>Mature_746_residues
GKDLIIVESPAKVKTIKKFLGNKYMVQASVGHIRDLPTKELGVDEENDFSPDYQVIQGKQKVVSALREAAAKADNVFLAP
DPDREGEAIAWHVAEIIAKENKNISRIQFNEITARAVREALENPRELNRSLFDAQQARRVLDRLVGYKISPILWNKIKRG
ISAGRVQSVALRLIVDRERERYAFTSEEYWVFRANLAAETPPPFKAELFKVDDKKPAIASAEQAQELEKSIEGKAFIVDS
VQEKERSRKPQPPFITSTLQQLASQRLGFSAKRTMSVAQRLYEGLDLGDMGTTALITYMRTDSVRISDEARDSARELIAS
LYGKEYCPAKAPVYKTKGSAQDAHEAIRPVDVTLTPDKIKGLVAPEQYSLYRLIWSRFMASQMAPARFHDTTVLIGCGGT
QWRVKGERLLFPGYMAAAPASAKEANVELPPMQQGQTLELTLLEKEQKFTQPPARYSEASLVREMEEKGIGRPSTYASII
STIVDRDYTRLEEKHFVPTDLGFVVCDMLTNHFGTLMDIGFTAQMENSLDKVAEGDLDWVALMKDFTSDFNPTLASAAKE
MKTVKAGLETDIVCSECGKPMAIKFGKAGPFLACTGYPDCKNTSNFTRDESGTIQLVEREQEELKPVGTCPECGKDLVVK
QARTGSRFIACTGYPDCKHTEPFSTGVPCPNDGCDGQLVEKSSRRGKIFYSCNRYPQCDYATWNWPVAEACPQCDSKILT
IKSTKARGKHIACPACKYTRALEDEE

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=563, Percent_Identity=24.3339253996448, Blast_Score=134, Evalue=3e-31,
Organism=Homo sapiens, GI4507635, Length=715, Percent_Identity=23.2167832167832, Blast_Score=120, Evalue=4e-27,
Organism=Escherichia coli, GI1787529, Length=789, Percent_Identity=41.06463878327, Blast_Score=542, Evalue=1e-155,
Organism=Escherichia coli, GI48994931, Length=163, Percent_Identity=39.8773006134969, Blast_Score=117, Evalue=3e-27,
Organism=Escherichia coli, GI1788061, Length=622, Percent_Identity=22.9903536977492, Blast_Score=93, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI32563869, Length=764, Percent_Identity=24.6073298429319, Blast_Score=142, Evalue=9e-34,
Organism=Caenorhabditis elegans, GI17555378, Length=514, Percent_Identity=24.124513618677, Blast_Score=107, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24585251, Length=582, Percent_Identity=24.3986254295533, Blast_Score=117, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24640096, Length=544, Percent_Identity=22.0588235294118, Blast_Score=103, Evalue=6e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 83315; Mature: 83184

Theoretical pI: Translated: 7.28; Mature: 7.28

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKDLIIVESPAKVKTIKKFLGNKYMVQASVGHIRDLPTKELGVDEENDFSPDYQVIQGK
CCCCEEEEECCHHHHHHHHHHCCCEEEEECCCHHHCCCCHHCCCCCCCCCCCCHHHHCCH
QKVVSALREAAAKADNVFLAPDPDREGEAIAWHVAEIIAKENKNISRIQFNEITARAVRE
HHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHH
ALENPRELNRSLFDAQQARRVLDRLVGYKISPILWNKIKRGISAGRVQSVALRLIVDRER
HHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCH
ERYAFTSEEYWVFRANLAAETPPPFKAELFKVDDKKPAIASAEQAQELEKSIEGKAFIVD
HHEEECCCCEEEEEECCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEC
SVQEKERSRKPQPPFITSTLQQLASQRLGFSAKRTMSVAQRLYEGLDLGDMGTTALITYM
CHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHEEEEE
RTDSVRISDEARDSARELIASLYGKEYCPAKAPVYKTKGSAQDAHEAIRPVDVTLTPDKI
ECCCEEECHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCCHHHHHHCCCEEEEECHHHH
KGLVAPEQYSLYRLIWSRFMASQMAPARFHDTTVLIGCGGTQWRVKGERLLFPGYMAAAP
CCCCCCCHHHHHHHHHHHHHHHHCCCCEECCCEEEEECCCCEEEEECCEEECCCHHHCCC
ASAKEANVELPPMQQGQTLELTLLEKEQKFTQPPARYSEASLVREMEEKGIGRPSTYASI
CCCCCCCCCCCCCCCCCEEEEEEECCHHHCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHH
ISTIVDRDYTRLEEKHFVPTDLGFVVCDMLTNHFGTLMDIGFTAQMENSLDKVAEGDLDW
HHHHHCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHH
VALMKDFTSDFNPTLASAAKEMKTVKAGLETDIVCSECGKPMAIKFGKAGPFLACTGYPD
HHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCCCEEEEECCCCCEEEECCCCC
CKNTSNFTRDESGTIQLVEREQEELKPVGTCPECGKDLVVKQARTGSRFIACTGYPDCKH
CCCCCCCCCCCCCCEEEEECCHHHCCCCCCCHHHCHHHHHHHCCCCCEEEEECCCCCCCC
TEPFSTGVPCPNDGCDGQLVEKSSRRGKIFYSCNRYPQCDYATWNWPVAEACPQCDSKIL
CCCCCCCCCCCCCCCCCHHHHCCCCCCEEEEECCCCCCCCCCCCCCCHHHHCCCCCCEEE
TIKSTKARGKHIACPACKYTRALEDEE
EEEECCCCCCEECCCHHHHHHHCCCCC
>Mature Secondary Structure 
GKDLIIVESPAKVKTIKKFLGNKYMVQASVGHIRDLPTKELGVDEENDFSPDYQVIQGK
CCCEEEEECCHHHHHHHHHHCCCEEEEECCCHHHCCCCHHCCCCCCCCCCCCHHHHCCH
QKVVSALREAAAKADNVFLAPDPDREGEAIAWHVAEIIAKENKNISRIQFNEITARAVRE
HHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHH
ALENPRELNRSLFDAQQARRVLDRLVGYKISPILWNKIKRGISAGRVQSVALRLIVDRER
HHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCH
ERYAFTSEEYWVFRANLAAETPPPFKAELFKVDDKKPAIASAEQAQELEKSIEGKAFIVD
HHEEECCCCEEEEEECCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEC
SVQEKERSRKPQPPFITSTLQQLASQRLGFSAKRTMSVAQRLYEGLDLGDMGTTALITYM
CHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHEEEEE
RTDSVRISDEARDSARELIASLYGKEYCPAKAPVYKTKGSAQDAHEAIRPVDVTLTPDKI
ECCCEEECHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCCHHHHHHCCCEEEEECHHHH
KGLVAPEQYSLYRLIWSRFMASQMAPARFHDTTVLIGCGGTQWRVKGERLLFPGYMAAAP
CCCCCCCHHHHHHHHHHHHHHHHCCCCEECCCEEEEECCCCEEEEECCEEECCCHHHCCC
ASAKEANVELPPMQQGQTLELTLLEKEQKFTQPPARYSEASLVREMEEKGIGRPSTYASI
CCCCCCCCCCCCCCCCCEEEEEEECCHHHCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHH
ISTIVDRDYTRLEEKHFVPTDLGFVVCDMLTNHFGTLMDIGFTAQMENSLDKVAEGDLDW
HHHHHCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHH
VALMKDFTSDFNPTLASAAKEMKTVKAGLETDIVCSECGKPMAIKFGKAGPFLACTGYPD
HHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCCCEEEEECCCCCEEEECCCCC
CKNTSNFTRDESGTIQLVEREQEELKPVGTCPECGKDLVVKQARTGSRFIACTGYPDCKH
CCCCCCCCCCCCCCEEEEECCHHHCCCCCCCHHHCHHHHHHHCCCCCEEEEECCCCCCCC
TEPFSTGVPCPNDGCDGQLVEKSSRRGKIFYSCNRYPQCDYATWNWPVAEACPQCDSKIL
CCCCCCCCCCCCCCCCCHHHHCCCCCCEEEEECCCCCCCCCCCCCCCHHHHCCCCCCEEE
TIKSTKARGKHIACPACKYTRALEDEE
EEEECCCCCCEECCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]