Click here to switch to the map view.

The map label for this gene is queA

Identifier: 78355089

GI number: 78355089

Start: 33471

End: 34613

Strand: Direct

Name: queA

Synonym: Dde_0042

Alternate gene names: 78355089

Gene position: 33471-34613 (Clockwise)

Preceding gene: 78355082

Following gene: 78355090

Centisome position: 0.9

GC content: 60.37

Gene sequence:

>1143_bases
ATGCATTCCAAGCATCCCACAGATACGGCCCGCCGCTGCGAGACAGGCACTGACAGCAGCGATACCGCCGCAGACGACTA
CTCTCTTGCCGGATACCGTTTTGAACTGCCCGAAGAACAGATAGCGCAGCATCCGCCGGAACACCGCGGGGCTTCGCGGC
TTTTTGTCATGGACAGGAAGTCCGGTGTTAACACACAGGCTTCTTTTAAGGATATAGGCAGCTTTTTGCCCGAGGGCGCC
CTGCTGGTGGCCAACAACTCGCGCGTGCTGCCCGCGCGCATGCTGGGCAGACGCCCCACCGGCGGCAAGGTGGAATTTCT
GCTGCTTACTCCGCTGCCGCTGGTACAGCCGCAGACCGGTACAGACGGCCGCAGCTGTGCAGAGGCAGAAGGTCTTCTGA
GAGCTTCCAAACGGGTGCGGCCTGGTGATGTCATGCATTTTGACGGGCTGGACGTTGAGGTGCTGCACACTGCCGACTTC
GGCCGCTGTGCGGTGCGCATGCACTGGAAAGGCGACCTTGCCGGACTGTTCCTGCGGCAGGGGCATCTGCCGCTGCCTCC
GTACATCCGCAGGCCCGACGGAGAAGATGACCACAGCAGATACCAGACCGTGTACAGCAGGCAGGACAGACTGGGCTCGG
TGGCGGCACCCACCGCGGGGCTGCATTTCACGCCGGAGCTTACGGCGTCGTTGCAGGAACAGGGATTCGGCTGGGCCGAG
GTCACGCTGTATGTCGGCTACGGCACGTTCAGCCCCGTCCGCGCAGAGGATATCCGTACCCATACCATGCACCGCGAGTA
CATGGAAATAACGGAGCAGGCCGCACAGGCCATAAACGATGCAAAACGGCAGGGCCGGCCGGTGGTCACGGTGGGCACCA
CATCCACAAGGGTGCTGGAAGGGGCATGGGCCGCATGCGGAGAAATTCGTCCTTTTACCGGCTGGACGGACATTTTTATC
TATCCGGGGTACCGCTTTAACGTGGCCGACCACATCATCACCAATTTTCATCTGCCGGAATCGTCATTGCTTATGATGAT
ATCGGCTTTTGCCGGGCGCGAAAAAACCCTGCAGGCCTACAGTCAGGCCGTGGCTGCAGGGTACCGCTTTTTCTCATACG
GCGACGCCATGCTTATTCTGTAA

Upstream 100 bases:

>100_bases
CCGAAAAAGACTTGCCTGCCGTGGCAAGTTCTGGTCTTTTGCGGCAATCCGCACCGGTGCGCCCGAAAGAACCGGAAAAC
GAATTAAGGACCATTGTTTT

Downstream 100 bases:

>100_bases
GTTTTATGGCACAAACCGCCGTTGCACAATTTTGCAAATTGCCGGGACTTGCGGTATACCCACGGCACTTAGAATTACCA
AAAAAACTCATGACACAGTG

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MHSKHPTDTARRCETGTDSSDTAADDYSLAGYRFELPEEQIAQHPPEHRGASRLFVMDRKSGVNTQASFKDIGSFLPEGA
LLVANNSRVLPARMLGRRPTGGKVEFLLLTPLPLVQPQTGTDGRSCAEAEGLLRASKRVRPGDVMHFDGLDVEVLHTADF
GRCAVRMHWKGDLAGLFLRQGHLPLPPYIRRPDGEDDHSRYQTVYSRQDRLGSVAAPTAGLHFTPELTASLQEQGFGWAE
VTLYVGYGTFSPVRAEDIRTHTMHREYMEITEQAAQAINDAKRQGRPVVTVGTTSTRVLEGAWAACGEIRPFTGWTDIFI
YPGYRFNVADHIITNFHLPESSLLMMISAFAGREKTLQAYSQAVAAGYRFFSYGDAMLIL

Sequences:

>Translated_380_residues
MHSKHPTDTARRCETGTDSSDTAADDYSLAGYRFELPEEQIAQHPPEHRGASRLFVMDRKSGVNTQASFKDIGSFLPEGA
LLVANNSRVLPARMLGRRPTGGKVEFLLLTPLPLVQPQTGTDGRSCAEAEGLLRASKRVRPGDVMHFDGLDVEVLHTADF
GRCAVRMHWKGDLAGLFLRQGHLPLPPYIRRPDGEDDHSRYQTVYSRQDRLGSVAAPTAGLHFTPELTASLQEQGFGWAE
VTLYVGYGTFSPVRAEDIRTHTMHREYMEITEQAAQAINDAKRQGRPVVTVGTTSTRVLEGAWAACGEIRPFTGWTDIFI
YPGYRFNVADHIITNFHLPESSLLMMISAFAGREKTLQAYSQAVAAGYRFFSYGDAMLIL
>Mature_380_residues
MHSKHPTDTARRCETGTDSSDTAADDYSLAGYRFELPEEQIAQHPPEHRGASRLFVMDRKSGVNTQASFKDIGSFLPEGA
LLVANNSRVLPARMLGRRPTGGKVEFLLLTPLPLVQPQTGTDGRSCAEAEGLLRASKRVRPGDVMHFDGLDVEVLHTADF
GRCAVRMHWKGDLAGLFLRQGHLPLPPYIRRPDGEDDHSRYQTVYSRQDRLGSVAAPTAGLHFTPELTASLQEQGFGWAE
VTLYVGYGTFSPVRAEDIRTHTMHREYMEITEQAAQAINDAKRQGRPVVTVGTTSTRVLEGAWAACGEIRPFTGWTDIFI
YPGYRFNVADHIITNFHLPESSLLMMISAFAGREKTLQAYSQAVAAGYRFFSYGDAMLIL

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=352, Percent_Identity=45.1704545454545, Blast_Score=280, Evalue=1e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_DESDG (Q317N3)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_386538.1
- STRING:   Q317N3
- GeneID:   3755122
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_0042
- NMPDR:   fig|207559.3.peg.548
- eggNOG:   COG0809
- HOGENOM:   HBG286076
- OMA:   ITRNPAP
- ProtClustDB:   PRK00147
- BioCyc:   DDES207559:DDE_0042-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 42133; Mature: 42133

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSKHPTDTARRCETGTDSSDTAADDYSLAGYRFELPEEQIAQHPPEHRGASRLFVMDRK
CCCCCCCCHHHHCCCCCCCCCCCCCCCEECCEEEECCHHHHHCCCCCCCCCCEEEEEECC
SGVNTQASFKDIGSFLPEGALLVANNSRVLPARMLGRRPTGGKVEFLLLTPLPLVQPQTG
CCCCCCHHHHHHHHHCCCCEEEEECCCEECHHHHHCCCCCCCCEEEEEECCCCCCCCCCC
TDGRSCAEAEGLLRASKRVRPGDVMHFDGLDVEVLHTADFGRCAVRMHWKGDLAGLFLRQ
CCCCHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEECCCCCCEEEEEEECCCHHHHHHCC
GHLPLPPYIRRPDGEDDHSRYQTVYSRQDRLGSVAAPTAGLHFTPELTASLQEQGFGWAE
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEECHHHHHHHHHCCCCEEE
VTLYVGYGTFSPVRAEDIRTHTMHREYMEITEQAAQAINDAKRQGRPVVTVGTTSTRVLE
EEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHH
GAWAACGEIRPFTGWTDIFIYPGYRFNVADHIITNFHLPESSLLMMISAFAGREKTLQAY
HHHHHHCCCCCCCCCEEEEEECCEEECHHHHHHEECCCCHHHHHHHHHHHCCCHHHHHHH
SQAVAAGYRFFSYGDAMLIL
HHHHHHHHHEEECCCEEEEC
>Mature Secondary Structure
MHSKHPTDTARRCETGTDSSDTAADDYSLAGYRFELPEEQIAQHPPEHRGASRLFVMDRK
CCCCCCCCHHHHCCCCCCCCCCCCCCCEECCEEEECCHHHHHCCCCCCCCCCEEEEEECC
SGVNTQASFKDIGSFLPEGALLVANNSRVLPARMLGRRPTGGKVEFLLLTPLPLVQPQTG
CCCCCCHHHHHHHHHCCCCEEEEECCCEECHHHHHCCCCCCCCEEEEEECCCCCCCCCCC
TDGRSCAEAEGLLRASKRVRPGDVMHFDGLDVEVLHTADFGRCAVRMHWKGDLAGLFLRQ
CCCCHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEECCCCCCEEEEEEECCCHHHHHHCC
GHLPLPPYIRRPDGEDDHSRYQTVYSRQDRLGSVAAPTAGLHFTPELTASLQEQGFGWAE
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEECHHHHHHHHHCCCCEEE
VTLYVGYGTFSPVRAEDIRTHTMHREYMEITEQAAQAINDAKRQGRPVVTVGTTSTRVLE
EEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHH
GAWAACGEIRPFTGWTDIFIYPGYRFNVADHIITNFHLPESSLLMMISAFAGREKTLQAY
HHHHHHCCCCCCCCCEEEEEECCEEECHHHHHHEECCCCHHHHHHHHHHHCCCHHHHHHH
SQAVAAGYRFFSYGDAMLIL
HHHHHHHHHEEECCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA