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The map label for this gene is hupR1 [H]

Identifier: 78224662

GI number: 78224662

Start: 3898624

End: 3900063

Strand: Reverse

Name: hupR1 [H]

Synonym: Gmet_3476

Alternate gene names: 78224662

Gene position: 3900063-3898624 (Counterclockwise)

Preceding gene: 78224663

Following gene: 78224661

Centisome position: 97.56

GC content: 58.54

Gene sequence:

>1440_bases
GTGCCAAAATTCTGTTCGTGGATGATGAAGAGCATATTCTCAACTCCTTGGAGCGGCTCTCCGCGGATATTTCCGCATTC
AAGAAAAGGGAATTATTGCCGAACGATAGTAAAGAGGGAGTACACGTTCATGTATGAACTGCCGGAAACTGCACCGGTAC
GGGTGCTTTTCGTCGATGACGAGGAAAACATGCTCAAGTCCCTGAGCAGGGTTTTCATGGATGAGGAGTTCGAGGTCATC
ACCGCTCCGTCGGCCAGGGAAGGACTGCGGATTCTCATGGAGACGGAGGACGTGGGGGTTATCGTGTCCGATCAGCGGAT
GCCCGAGATCACCGGGGTCCAGTTTCTGGCCCAGGTCCGGGAGATGTTCCCCGAGACACTCCGGATCATGCTGACCGGAT
ACGCAGACATTTCGGCAACTGTGGATGCCATCAATCGCGGCGGCGCCTATCGGTACATCACCAAGCCGTGGAACGACGAG
GAACTGGTCCAGATCATCCGCGACGCGGTCAACCAGTACCGGCTCGTTCAGGAGAACCGGCGCCTCACGGAGATCGTCAA
CAAGCAGAACGAGGAGCTCAAGGAGTGGAATGAGAACCTGAAGGTGCGGGTCCTCGAGCAGACTACCGAGATCCGGAAGA
AACACGAAAACCTGAAAGAGCTCAACGAACGGCTCCGGAGCAATTTCAAGGACACCGTCACGGCGTTTTCCCGGCTTATA
GAACTGGGGGGGAAAAAGCGTCACGCCGAAACCGTGGCGACCCTCGCCATGAACATTGCCCAGGCAATGGGGGTTTCCGC
CGGGGAGGTGGAGACGGTGCACAACGCGGCCCTGCTCCACGACATCGGGGAGTTGGGGATAGCTGATGCGATCCTGAAGA
AGGACCTGTCAGAGATGACTCCGGGAGAGCTGAGAGAGTACATGCTCCATGCCGTGAGGGGGCAGACGGCCATTGACGCC
ATTGAGGAGCTCACCCCTGCCGGGGTCCTCATCCGCCATCACCACGAGCATTACGACGGCAGCGGTTACCCCGACGGCCT
GCGGGGAGAGGCTATTCCCCTCGGCTCCCGCATCATCGCCATAGCCGATTTTCTCGATAGGGCCATACAGAAGCTGCACG
GTTACACTGCCATCGACTACGGCCTGCGGAGCGTCGCCGCCGAGTTGGGGAAGAAGCTCGATCCGTCCCTCTATGAGTCC
CTGCGCAAGTTCGCCAAGCACGTCTACTATGTGAAGGAAACGGATACCAGGAAACTCGGCGAGGTCGAGGTGGGAATCGC
CGAGCTTTTTGTCGGCCAAGTGCTCTCCCGCGACATATACAGTGGCACCGGCCTCCTTCTCCTCAACCGGGGGGTACGCC
TCGATGTGGTCAAGATCGAGGCGATCCGCCGCTACTACCGCCTCGACCCTCCCCCTCACGGCGTCTTCGTGGAAGTATGA

Upstream 100 bases:

>100_bases
ATTGGCCGGTGTCGCCGGAGTCCGGAGTCTGGGGGTGTATCTCAAGGAGGGGGAGCAGGAGGGAATCGTTCTTGTCTTCA
ATGGGGAGGAACCCCTATGA

Downstream 100 bases:

>100_bases
GCGTGGGTAATGATTGAATACGACGAAGCACAAAAAAAGATGGTCTTGAAGCTGGTCTACTACGGACCAGCCATGTCGGG
AAAGACTACAAATCTACTGA

Product: response regulator receiver modulated metal dependent phosphohydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MPKFCSWMMKSIFSTPWSGSPRIFPHSRKGNYCRTIVKREYTFMYELPETAPVRVLFVDDEENMLKSLSRVFMDEEFEVI
TAPSAREGLRILMETEDVGVIVSDQRMPEITGVQFLAQVREMFPETLRIMLTGYADISATVDAINRGGAYRYITKPWNDE
ELVQIIRDAVNQYRLVQENRRLTEIVNKQNEELKEWNENLKVRVLEQTTEIRKKHENLKELNERLRSNFKDTVTAFSRLI
ELGGKKRHAETVATLAMNIAQAMGVSAGEVETVHNAALLHDIGELGIADAILKKDLSEMTPGELREYMLHAVRGQTAIDA
IEELTPAGVLIRHHHEHYDGSGYPDGLRGEAIPLGSRIIAIADFLDRAIQKLHGYTAIDYGLRSVAAELGKKLDPSLYES
LRKFAKHVYYVKETDTRKLGEVEVGIAELFVGQVLSRDIYSGTGLLLLNRGVRLDVVKIEAIRRYYRLDPPPHGVFVEV

Sequences:

>Translated_479_residues
MPKFCSWMMKSIFSTPWSGSPRIFPHSRKGNYCRTIVKREYTFMYELPETAPVRVLFVDDEENMLKSLSRVFMDEEFEVI
TAPSAREGLRILMETEDVGVIVSDQRMPEITGVQFLAQVREMFPETLRIMLTGYADISATVDAINRGGAYRYITKPWNDE
ELVQIIRDAVNQYRLVQENRRLTEIVNKQNEELKEWNENLKVRVLEQTTEIRKKHENLKELNERLRSNFKDTVTAFSRLI
ELGGKKRHAETVATLAMNIAQAMGVSAGEVETVHNAALLHDIGELGIADAILKKDLSEMTPGELREYMLHAVRGQTAIDA
IEELTPAGVLIRHHHEHYDGSGYPDGLRGEAIPLGSRIIAIADFLDRAIQKLHGYTAIDYGLRSVAAELGKKLDPSLYES
LRKFAKHVYYVKETDTRKLGEVEVGIAELFVGQVLSRDIYSGTGLLLLNRGVRLDVVKIEAIRRYYRLDPPPHGVFVEV
>Mature_478_residues
PKFCSWMMKSIFSTPWSGSPRIFPHSRKGNYCRTIVKREYTFMYELPETAPVRVLFVDDEENMLKSLSRVFMDEEFEVIT
APSAREGLRILMETEDVGVIVSDQRMPEITGVQFLAQVREMFPETLRIMLTGYADISATVDAINRGGAYRYITKPWNDEE
LVQIIRDAVNQYRLVQENRRLTEIVNKQNEELKEWNENLKVRVLEQTTEIRKKHENLKELNERLRSNFKDTVTAFSRLIE
LGGKKRHAETVATLAMNIAQAMGVSAGEVETVHNAALLHDIGELGIADAILKKDLSEMTPGELREYMLHAVRGQTAIDAI
EELTPAGVLIRHHHEHYDGSGYPDGLRGEAIPLGSRIIAIADFLDRAIQKLHGYTAIDYGLRSVAAELGKKLDPSLYESL
RKFAKHVYYVKETDTRKLGEVEVGIAELFVGQVLSRDIYSGTGLLLLNRGVRLDVVKIEAIRRYYRLDPPPHGVFVEV

Specific function: Probable member of the two-component regulatory system involved in the regulation of the [NiFe] hydrogenase activity. HupR1 is probably phosphorylated by the sensory component and then acts in conjunction with sigma-54 as a transcriptional activator [H]

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=138, Percent_Identity=33.3333333333333, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1788905, Length=157, Percent_Identity=33.7579617834395, Blast_Score=75, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 54535; Mature: 54404

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKFCSWMMKSIFSTPWSGSPRIFPHSRKGNYCRTIVKREYTFMYELPETAPVRVLFVDD
CCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHEEEEECCCCCCEEEEEECC
EENMLKSLSRVFMDEEFEVITAPSAREGLRILMETEDVGVIVSDQRMPEITGVQFLAQVR
HHHHHHHHHHHHCCCCCEEEECCCHHHCCEEEEEECCCEEEECCCCCCHHHHHHHHHHHH
EMFPETLRIMLTGYADISATVDAINRGGAYRYITKPWNDEELVQIIRDAVNQYRLVQENR
HHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
RLTEIVNKQNEELKEWNENLKVRVLEQTTEIRKKHENLKELNERLRSNFKDTVTAFSRLI
HHHHHHCCCHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHH
ELGGKKRHAETVATLAMNIAQAMGVSAGEVETVHNAALLHDIGELGIADAILKKDLSEMT
HHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PGELREYMLHAVRGQTAIDAIEELTPAGVLIRHHHEHYDGSGYPDGLRGEAIPLGSRIIA
HHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHH
IADFLDRAIQKLHGYTAIDYGLRSVAAELGKKLDPSLYESLRKFAKHVYYVKETDTRKLG
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHEEEEECCCCCCCC
EVEVGIAELFVGQVLSRDIYSGTGLLLLNRGVRLDVVKIEAIRRYYRLDPPPHGVFVEV
CHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEHHHHHHHHHHCCCCCCCCEEEEC
>Mature Secondary Structure 
PKFCSWMMKSIFSTPWSGSPRIFPHSRKGNYCRTIVKREYTFMYELPETAPVRVLFVDD
CHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHEEEEECCCCCCEEEEEECC
EENMLKSLSRVFMDEEFEVITAPSAREGLRILMETEDVGVIVSDQRMPEITGVQFLAQVR
HHHHHHHHHHHHCCCCCEEEECCCHHHCCEEEEEECCCEEEECCCCCCHHHHHHHHHHHH
EMFPETLRIMLTGYADISATVDAINRGGAYRYITKPWNDEELVQIIRDAVNQYRLVQENR
HHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
RLTEIVNKQNEELKEWNENLKVRVLEQTTEIRKKHENLKELNERLRSNFKDTVTAFSRLI
HHHHHHCCCHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHH
ELGGKKRHAETVATLAMNIAQAMGVSAGEVETVHNAALLHDIGELGIADAILKKDLSEMT
HHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PGELREYMLHAVRGQTAIDAIEELTPAGVLIRHHHEHYDGSGYPDGLRGEAIPLGSRIIA
HHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHH
IADFLDRAIQKLHGYTAIDYGLRSVAAELGKKLDPSLYESLRKFAKHVYYVKETDTRKLG
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHEEEEECCCCCCCC
EVEVGIAELFVGQVLSRDIYSGTGLLLLNRGVRLDVVKIEAIRRYYRLDPPPHGVFVEV
CHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEHHHHHHHHHHCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1885559; 8497190; 2007559 [H]