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The map label for this gene is hupR1 [H]

Identifier: 78224658

GI number: 78224658

Start: 3893506

End: 3894069

Strand: Reverse

Name: hupR1 [H]

Synonym: Gmet_3472

Alternate gene names: 78224658

Gene position: 3894069-3893506 (Counterclockwise)

Preceding gene: 78224659

Following gene: 78224653

Centisome position: 97.41

GC content: 57.09

Gene sequence:

>564_bases
ATGAAAAAGACCCTGCTGCTGGTGGATGACGAGCCCTTTGTCATTAATTCGCTCCGGAGGGCCCTCGCGGACGAGCCCTA
CGAGATACTGACTGCCGGAAGCGGTCAAGAGGGGTTGGCGCTTTTCGCTGAGCACCGGGTCGGTGTGGTGGTGTCCGACG
AGAAGATGCCGGGCATGGGGGGAGCCGAATTTCTTGCTGCTGTCCGCGAGCGGTACCCCGAGACGATCAGGATCATGCTC
ACGGGTCATGCCTCTATCGATGCCACCATGAGGGCGGTGAACCAGGGGGAAATCTACCGCTTCTTCACCAAGCCGTGGGA
TGACCTGGAGTTGAGATTTGCGCTACGCTCAGCTTTTGACAGGTTGGTGCTCGAAGAGGATAACCGGCGTCTCCTGCGAA
CGGTGAAACGCCAGAGCCAGGAACTGAGCGCTCTGGAACAGAAGTACCCCGGGATCACCGACGTGGAGAAAGATGCCGAT
GGCGCTCTGGTTCTGCCGGAGATTTCGGTTGAAGACATCAACAATTTCATTGAACGATGCAACAGAGAGTATCAGAAGAC
CTGA

Upstream 100 bases:

>100_bases
GCTGTAAGGGATGCAAGATGCCCCTGGAGGAGGAGTGGTCCGTTTGCCCCTTCTGCGGGGTGGAGAAGGGGACGGACAGC
CTGACTTTGATGGAGGAAAT

Downstream 100 bases:

>100_bases
CGGGTTTGTGGAGACATTGCACGGTTGCACGGTTTGTCACGGGGCATTTCGGGGTGGGTCTGCCTTTGAAGCCGATCAAC
ATCAACGATTTGTTAATTTG

Product: response regulator receiver domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 187; Mature: 187

Protein sequence:

>187_residues
MKKTLLLVDDEPFVINSLRRALADEPYEILTAGSGQEGLALFAEHRVGVVVSDEKMPGMGGAEFLAAVRERYPETIRIML
TGHASIDATMRAVNQGEIYRFFTKPWDDLELRFALRSAFDRLVLEEDNRRLLRTVKRQSQELSALEQKYPGITDVEKDAD
GALVLPEISVEDINNFIERCNREYQKT

Sequences:

>Translated_187_residues
MKKTLLLVDDEPFVINSLRRALADEPYEILTAGSGQEGLALFAEHRVGVVVSDEKMPGMGGAEFLAAVRERYPETIRIML
TGHASIDATMRAVNQGEIYRFFTKPWDDLELRFALRSAFDRLVLEEDNRRLLRTVKRQSQELSALEQKYPGITDVEKDAD
GALVLPEISVEDINNFIERCNREYQKT
>Mature_187_residues
MKKTLLLVDDEPFVINSLRRALADEPYEILTAGSGQEGLALFAEHRVGVVVSDEKMPGMGGAEFLAAVRERYPETIRIML
TGHASIDATMRAVNQGEIYRFFTKPWDDLELRFALRSAFDRLVLEEDNRRLLRTVKRQSQELSALEQKYPGITDVEKDAD
GALVLPEISVEDINNFIERCNREYQKT

Specific function: Probable member of the two-component regulatory system involved in the regulation of the [NiFe] hydrogenase activity. HupR1 is probably phosphorylated by the sensory component and then acts in conjunction with sigma-54 as a transcriptional activator [H]

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788905, Length=143, Percent_Identity=36.3636363636364, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1788550, Length=166, Percent_Identity=28.3132530120482, Blast_Score=63, Evalue=1e-11,
Organism=Escherichia coli, GI1790299, Length=106, Percent_Identity=33.0188679245283, Blast_Score=61, Evalue=6e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 21292; Mature: 21292

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTLLLVDDEPFVINSLRRALADEPYEILTAGSGQEGLALFAEHRVGVVVSDEKMPGMG
CCCEEEEECCCCHHHHHHHHHHCCCCCEEEECCCCCCCCEEEECCCEEEEECCCCCCCCC
GAEFLAAVRERYPETIRIMLTGHASIDATMRAVNQGEIYRFFTKPWDDLELRFALRSAFD
HHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHH
RLVLEEDNRRLLRTVKRQSQELSALEQKYPGITDVEKDADGALVLPEISVEDINNFIERC
HHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHH
NREYQKT
HHHHCCC
>Mature Secondary Structure
MKKTLLLVDDEPFVINSLRRALADEPYEILTAGSGQEGLALFAEHRVGVVVSDEKMPGMG
CCCEEEEECCCCHHHHHHHHHHCCCCCEEEECCCCCCCCEEEECCCEEEEECCCCCCCCC
GAEFLAAVRERYPETIRIMLTGHASIDATMRAVNQGEIYRFFTKPWDDLELRFALRSAFD
HHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHH
RLVLEEDNRRLLRTVKRQSQELSALEQKYPGITDVEKDADGALVLPEISVEDINNFIERC
HHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHH
NREYQKT
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1885559; 8497190; 2007559 [H]