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The map label for this gene is tolC [H]

Identifier: 78224652

GI number: 78224652

Start: 3880184

End: 3881509

Strand: Reverse

Name: tolC [H]

Synonym: Gmet_3465

Alternate gene names: 78224652

Gene position: 3881509-3880184 (Counterclockwise)

Preceding gene: 78224653

Following gene: 78224651

Centisome position: 97.1

GC content: 63.42

Gene sequence:

>1326_bases
ATGAAAAGGTTAGTGATCTTGATGGGGATGGTTCTTCTGCCGCATGGAGTCTTTGCAGAGGCTGGCGTGCCGGCCTTCGC
CCTTTCCCGGGTGATTGATTTCGCCCTGGAGAACGAGCCGAAGCTTTCGGTCTTGGGCAAGGATATCGAGGCGGCAAAAC
ACACCGTCGACGCCGCCAAGGGGGGGAGGTGGCCAAGGCTCGATCTGGAGGCAGGCATCACGCGCTACCGCTATGCCGCT
CCCATCACCCCTATCTCCGGGGCCCCGCAGAATGGCGGAGGATTCCCCCCCTTCGACAAAGACATCTACGATGCGTCGCT
GACCCTGAAGCTTCCCATCTACCGGGGGGGACGCCTGGTGAAAAACGTGGAGATTGCGGAGATCAACCGGCTGATTGCGG
AGCGGGCCCTTCATCAGGGGAGACAGGAGCTGGTCTTCAACGTCACGAGCGTCTACTGCAAGATCCTGGAGCTGGAGGAG
CTCCTCAAGGCCCACGAGCAGAATGTGCGGCAACTCGAAGAGCACCGGCGAGTCACCGAGCAGTTTGTCAAGGCCGGGAG
CGCGGCGCGGGTGGACCTCATGAAGACCGACGTGGAGCTCGCCCATGGCCGTCAGCAGGTCCTGGCGACGCAAAACGCCG
GCGCTGCCGCCTATGCGCTGCTGCGCTCCCTCATGGGGGTGGACGAAAGCTACCCGCGATTCACCGTGGCCGAAGAGCCT
TTCCCTGAAAAGGGGTATCCGCCTGAAGAAAAAGCGGTCGAGGTCGCCCTGCGGCAGCGTCCCGACTACCTGAAGGTAAA
GGCGCGGACGGAACTTTTCCAGGCACGGCTCGACCTCGCCAGGGGGAAACGCCTCCCATCCGTGGATGTGGCGGGGGAGT
ACGGGGAAAGGGCGGGGGATTCCTTGTCGTTCAAGGAGAACTGGAACATCGGTCTTCGGCTCTCCTACCCCCTCTTTGAC
GGCGGGATAACCAGAGCGGAGATCAAAAAGGGGATGGTGGAGCTGGAGCGGACCGGCGAAGAGCTCCGTGCCGTGCGGCT
TTCGATCGTCCGGGAGGTGAGGGAGGCGTATCTTGCCGTCGCCGACGCGGAGAAGAGGCTTGCCGTGGCGCGGGAGGCGA
TCTCCTCGGCGGAAGAGAACCTGCGGGTAGAGCAGTTGAAGTATGAAACCGGGGTCGGGACGACGAGGGACGTGATCGAC
GCCCAGACGGCGCTCCTGCGGGCAACGACCGACCGGCACCAGGCCTCCTACGACTTGGCGGCGGCGAAGGCGGCCCTGGA
CAAGGCCTTGGGGAGCAACGCATACGGGGAGGTGGCCTCACGATGA

Upstream 100 bases:

>100_bases
CCTTTTCCCTGGTCAATGAAGGGATGAAGCTCTGGCGCAATTACGAGGAATATCTGACTTCGGGGATTTTGCGGACTCAA
CCTTCATAACGGAGACGGGC

Downstream 100 bases:

>100_bases
AAAGGAAACTGATACTTCTAATACTTGTGGTCGTCATTGCGGGTGTTGCCGCCTTCTTCCTGCTGAATCGGAAAAAGGAC
GATACCGGTATCCTGCTAAG

Product: Outer membrane efflux protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 441; Mature: 441

Protein sequence:

>441_residues
MKRLVILMGMVLLPHGVFAEAGVPAFALSRVIDFALENEPKLSVLGKDIEAAKHTVDAAKGGRWPRLDLEAGITRYRYAA
PITPISGAPQNGGGFPPFDKDIYDASLTLKLPIYRGGRLVKNVEIAEINRLIAERALHQGRQELVFNVTSVYCKILELEE
LLKAHEQNVRQLEEHRRVTEQFVKAGSAARVDLMKTDVELAHGRQQVLATQNAGAAAYALLRSLMGVDESYPRFTVAEEP
FPEKGYPPEEKAVEVALRQRPDYLKVKARTELFQARLDLARGKRLPSVDVAGEYGERAGDSLSFKENWNIGLRLSYPLFD
GGITRAEIKKGMVELERTGEELRAVRLSIVREVREAYLAVADAEKRLAVAREAISSAEENLRVEQLKYETGVGTTRDVID
AQTALLRATTDRHQASYDLAAAKAALDKALGSNAYGEVASR

Sequences:

>Translated_441_residues
MKRLVILMGMVLLPHGVFAEAGVPAFALSRVIDFALENEPKLSVLGKDIEAAKHTVDAAKGGRWPRLDLEAGITRYRYAA
PITPISGAPQNGGGFPPFDKDIYDASLTLKLPIYRGGRLVKNVEIAEINRLIAERALHQGRQELVFNVTSVYCKILELEE
LLKAHEQNVRQLEEHRRVTEQFVKAGSAARVDLMKTDVELAHGRQQVLATQNAGAAAYALLRSLMGVDESYPRFTVAEEP
FPEKGYPPEEKAVEVALRQRPDYLKVKARTELFQARLDLARGKRLPSVDVAGEYGERAGDSLSFKENWNIGLRLSYPLFD
GGITRAEIKKGMVELERTGEELRAVRLSIVREVREAYLAVADAEKRLAVAREAISSAEENLRVEQLKYETGVGTTRDVID
AQTALLRATTDRHQASYDLAAAKAALDKALGSNAYGEVASR
>Mature_441_residues
MKRLVILMGMVLLPHGVFAEAGVPAFALSRVIDFALENEPKLSVLGKDIEAAKHTVDAAKGGRWPRLDLEAGITRYRYAA
PITPISGAPQNGGGFPPFDKDIYDASLTLKLPIYRGGRLVKNVEIAEINRLIAERALHQGRQELVFNVTSVYCKILELEE
LLKAHEQNVRQLEEHRRVTEQFVKAGSAARVDLMKTDVELAHGRQQVLATQNAGAAAYALLRSLMGVDESYPRFTVAEEP
FPEKGYPPEEKAVEVALRQRPDYLKVKARTELFQARLDLARGKRLPSVDVAGEYGERAGDSLSFKENWNIGLRLSYPLFD
GGITRAEIKKGMVELERTGEELRAVRLSIVREVREAYLAVADAEKRLAVAREAISSAEENLRVEQLKYETGVGTTRDVID
AQTALLRATTDRHQASYDLAAAKAALDKALGSNAYGEVASR

Specific function: May be specialized for signal sequence independent, extracellular secretion in Gram-negative bacteria [H]

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI87082199, Length=419, Percent_Identity=22.673031026253, Blast_Score=70, Evalue=4e-13,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010130 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 48759; Mature: 48759

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLVILMGMVLLPHGVFAEAGVPAFALSRVIDFALENEPKLSVLGKDIEAAKHTVDAAK
CCHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHCCCCCCEECCCHHHHHHHHHHHHC
GGRWPRLDLEAGITRYRYAAPITPISGAPQNGGGFPPFDKDIYDASLTLKLPIYRGGRLV
CCCCCCEEHHHCCHHHEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCEEE
KNVEIAEINRLIAERALHQGRQELVFNVTSVYCKILELEELLKAHEQNVRQLEEHRRVTE
ECCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QFVKAGSAARVDLMKTDVELAHGRQQVLATQNAGAAAYALLRSLMGVDESYPRFTVAEEP
HHHHCCCCCEEHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCEEECCCC
FPEKGYPPEEKAVEVALRQRPDYLKVKARTELFQARLDLARGKRLPSVDVAGEYGERAGD
CCCCCCCCHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCC
SLSFKENWNIGLRLSYPLFDGGITRAEIKKGMVELERTGEELRAVRLSIVREVREAYLAV
CCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADAEKRLAVAREAISSAEENLRVEQLKYETGVGTTRDVIDAQTALLRATTDRHQASYDLA
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AAKAALDKALGSNAYGEVASR
HHHHHHHHHHCCCCCHHHCCC
>Mature Secondary Structure
MKRLVILMGMVLLPHGVFAEAGVPAFALSRVIDFALENEPKLSVLGKDIEAAKHTVDAAK
CCHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHCCCCCCEECCCHHHHHHHHHHHHC
GGRWPRLDLEAGITRYRYAAPITPISGAPQNGGGFPPFDKDIYDASLTLKLPIYRGGRLV
CCCCCCEEHHHCCHHHEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCEEE
KNVEIAEINRLIAERALHQGRQELVFNVTSVYCKILELEELLKAHEQNVRQLEEHRRVTE
ECCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QFVKAGSAARVDLMKTDVELAHGRQQVLATQNAGAAAYALLRSLMGVDESYPRFTVAEEP
HHHHCCCCCEEHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCEEECCCC
FPEKGYPPEEKAVEVALRQRPDYLKVKARTELFQARLDLARGKRLPSVDVAGEYGERAGD
CCCCCCCCHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCC
SLSFKENWNIGLRLSYPLFDGGITRAEIKKGMVELERTGEELRAVRLSIVREVREAYLAV
CCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADAEKRLAVAREAISSAEENLRVEQLKYETGVGTTRDVIDAQTALLRATTDRHQASYDLA
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AAKAALDKALGSNAYGEVASR
HHHHHHHHHHCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]