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The map label for this gene is rimO

Identifier: 78224396

GI number: 78224396

Start: 3604842

End: 3606179

Strand: Reverse

Name: rimO

Synonym: Gmet_3205

Alternate gene names: 78224396

Gene position: 3606179-3604842 (Counterclockwise)

Preceding gene: 78224397

Following gene: 78224395

Centisome position: 90.21

GC content: 62.93

Gene sequence:

>1338_bases
ATGAAGGAAAAAGTTAGCATGGTCAGCCTCGGCTGCCCGAAGAACCTGGTGGACGCCGAGGTGATGCTCGGCTGCCTGGC
CAAGGACCAGTACGAGATCACCACCGACGAACACGAGGCGGACATCATCATCGTCAACACCTGCTCCTTTATTGGCGATG
CCAAGAAGGAGAGCATCGACACGATCCTCGATCTGGCCGACCGCAAGCACGACGGCCGCTGCAAGCTCCTGGTCGTGACC
GGCTGCCTCCCCCAGCGCTACCAGGAGGAACTGGCCACGGAGCTCCCCGAGGTGGATATCTTCGTGGGGACCGCCGACTA
TCCCCGCATTGCCGAGATCATTGCCGAGAAGCGGGGCGTTGCCGAGCAGCTCCGTTACATCAGCGACCCCAACTACATCT
TCGACGAGAGCCTGCCGCGGCTCAAGTCGTCCCCCGCCTACACCGCCTACCTGAAAATCGCCGAGGGGTGCTCCAACTGC
TGCTCCTACTGCGTCATCCCTTCGCTGCGGGGTGCCCTCCGCTCCCGCCCCCTGGACAACCTCCTCATGGAGGCCCGGAC
TCTGGTCGCCACCGGCACCAGAGAGATTAATCTCATTGCCCAGGACATCACCGCCTGGGGGCGCGACCTGCCGGGAAGCC
CCACCCTGGAGGAGCTTGTCCGGGAACTGGCGAAGATCGTGGGACTGCGCTGGATCAGGCTTCTCTACGCCTACCCCGAC
GGGATCACCGACGGGCTCATCGAGATCATCAAGACCGAGCCCAAGGTCTGCAAGTACCTGGATATCCCGATCCAGCACAT
CAGCGATCCGGTCCTGAAGCGGATGAACCGCCGGAGCACCGAGCCCCAGATCCGGGCGCTCATCGCGAAGATCCGCGCCG
AGATCCCCGAGATCGCGCTCCGCACCTCCCTCATCGTCGGCTTCCCCGGCGAGACCGAGGAGGATTTCCGCACCCTTCTC
CACTACGTGGAGGAGACCCAGTTCGACCGTCTTGGCGTCTTCTGCTACTCACGGGAGGAGGGGACACCGGCTGCCGGGAT
GGAGGACCAGGTTTCGGAGCGGGTCAAGCGGGACCGCTACAAGAAGCTCATGAAGGTTCAGGCGAGGCTCTCCTTCAAGC
GGAACCGCCGCCTCATCGATACCGAGGAGGAAGTGATCGTGGAGGGGTACAGCGACGAGACCGACCTCCTCCTCAAGGGT
CGTTCGTCCCGGCAGGCCCCCGACATCGACGGCCAGGTCTACATCACCGCCGGCAATGCCAACGTGGGGGATATTGTGCG
GCTGCGGATCACCGATTCGTCCGACTATGACCTCATCGGCGAGATTGTTGAAGAGTAA

Upstream 100 bases:

>100_bases
GGCGATCCGGACCCTCAAGGGGAAGGATCTGGGGATCGAGCTTCAGTTTGTGAATTTCAGGGATTAGACGTTACGGAAAC
AGAGAGAGGGAGCGGATTTG

Downstream 100 bases:

>100_bases
TGGGCCTGAAATTTGCTTGACAATCAAAAGTTTTTGAGTAACATTTCGAGACCTTTTGGCCCACCCTATAACGATGGAGT
GATGTAAATGGTTGAAAAAC

Product: hypothetical protein

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MKEKVSMVSLGCPKNLVDAEVMLGCLAKDQYEITTDEHEADIIIVNTCSFIGDAKKESIDTILDLADRKHDGRCKLLVVT
GCLPQRYQEELATELPEVDIFVGTADYPRIAEIIAEKRGVAEQLRYISDPNYIFDESLPRLKSSPAYTAYLKIAEGCSNC
CSYCVIPSLRGALRSRPLDNLLMEARTLVATGTREINLIAQDITAWGRDLPGSPTLEELVRELAKIVGLRWIRLLYAYPD
GITDGLIEIIKTEPKVCKYLDIPIQHISDPVLKRMNRRSTEPQIRALIAKIRAEIPEIALRTSLIVGFPGETEEDFRTLL
HYVEETQFDRLGVFCYSREEGTPAAGMEDQVSERVKRDRYKKLMKVQARLSFKRNRRLIDTEEEVIVEGYSDETDLLLKG
RSSRQAPDIDGQVYITAGNANVGDIVRLRITDSSDYDLIGEIVEE

Sequences:

>Translated_445_residues
MKEKVSMVSLGCPKNLVDAEVMLGCLAKDQYEITTDEHEADIIIVNTCSFIGDAKKESIDTILDLADRKHDGRCKLLVVT
GCLPQRYQEELATELPEVDIFVGTADYPRIAEIIAEKRGVAEQLRYISDPNYIFDESLPRLKSSPAYTAYLKIAEGCSNC
CSYCVIPSLRGALRSRPLDNLLMEARTLVATGTREINLIAQDITAWGRDLPGSPTLEELVRELAKIVGLRWIRLLYAYPD
GITDGLIEIIKTEPKVCKYLDIPIQHISDPVLKRMNRRSTEPQIRALIAKIRAEIPEIALRTSLIVGFPGETEEDFRTLL
HYVEETQFDRLGVFCYSREEGTPAAGMEDQVSERVKRDRYKKLMKVQARLSFKRNRRLIDTEEEVIVEGYSDETDLLLKG
RSSRQAPDIDGQVYITAGNANVGDIVRLRITDSSDYDLIGEIVEE
>Mature_445_residues
MKEKVSMVSLGCPKNLVDAEVMLGCLAKDQYEITTDEHEADIIIVNTCSFIGDAKKESIDTILDLADRKHDGRCKLLVVT
GCLPQRYQEELATELPEVDIFVGTADYPRIAEIIAEKRGVAEQLRYISDPNYIFDESLPRLKSSPAYTAYLKIAEGCSNC
CSYCVIPSLRGALRSRPLDNLLMEARTLVATGTREINLIAQDITAWGRDLPGSPTLEELVRELAKIVGLRWIRLLYAYPD
GITDGLIEIIKTEPKVCKYLDIPIQHISDPVLKRMNRRSTEPQIRALIAKIRAEIPEIALRTSLIVGFPGETEEDFRTLL
HYVEETQFDRLGVFCYSREEGTPAAGMEDQVSERVKRDRYKKLMKVQARLSFKRNRRLIDTEEEVIVEGYSDETDLLLKG
RSSRQAPDIDGQVYITAGNANVGDIVRLRITDSSDYDLIGEIVEE

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=478, Percent_Identity=27.6150627615063, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI28872782, Length=478, Percent_Identity=27.6150627615063, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI93277076, Length=451, Percent_Identity=28.8248337028825, Blast_Score=161, Evalue=1e-39,
Organism=Escherichia coli, GI1787057, Length=454, Percent_Identity=38.1057268722467, Blast_Score=284, Evalue=9e-78,
Organism=Escherichia coli, GI1786882, Length=447, Percent_Identity=32.4384787472036, Blast_Score=227, Evalue=1e-60,
Organism=Caenorhabditis elegans, GI17553146, Length=473, Percent_Identity=26.4270613107822, Blast_Score=167, Evalue=7e-42,
Organism=Caenorhabditis elegans, GI71996771, Length=370, Percent_Identity=29.4594594594595, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI21356207, Length=479, Percent_Identity=27.5574112734864, Blast_Score=190, Evalue=2e-48,
Organism=Drosophila melanogaster, GI19922432, Length=469, Percent_Identity=28.3582089552239, Blast_Score=147, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMO_GEOMG (Q39QQ6)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_386143.1
- ProteinModelPortal:   Q39QQ6
- SMR:   Q39QQ6
- STRING:   Q39QQ6
- GeneID:   3740960
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_3205
- NMPDR:   fig|269799.3.peg.2439
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   KADAPEI
- PhylomeDB:   Q39QQ6
- ProtClustDB:   CLSK829158
- BioCyc:   GMET269799:GMET_3205-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01865
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01125
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 50210; Mature: 50210

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEKVSMVSLGCPKNLVDAEVMLGCLAKDQYEITTDEHEADIIIVNTCSFIGDAKKESID
CCCCCCEEECCCCCCHHHHHHHHHHHCCCCEEECCCCCCCCEEEEECCHHHCCHHHHHHH
TILDLADRKHDGRCKLLVVTGCLPQRYQEELATELPEVDIFVGTADYPRIAEIIAEKRGV
HHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCH
AEQLRYISDPNYIFDESLPRLKSSPAYTAYLKIAEGCSNCCSYCVIPSLRGALRSRPLDN
HHHHHHCCCCCEECCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
LLMEARTLVATGTREINLIAQDITAWGRDLPGSPTLEELVRELAKIVGLRWIRLLYAYPD
HHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
GITDGLIEIIKTEPKVCKYLDIPIQHISDPVLKRMNRRSTEPQIRALIAKIRAEIPEIAL
CHHHHHHHHHHCCCHHHHHCCCCHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
RTSLIVGFPGETEEDFRTLLHYVEETQFDRLGVFCYSREEGTPAAGMEDQVSERVKRDRY
HHEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHH
KKLMKVQARLSFKRNRRLIDTEEEVIVEGYSDETDLLLKGRSSRQAPDIDGQVYITAGNA
HHHHHHHHHHHHHHCCCCCCCCHHHEEECCCCCCCEEEECCCCCCCCCCCCEEEEEECCC
NVGDIVRLRITDSSDYDLIGEIVEE
CCCCEEEEEEECCCCHHHHHHHHCC
>Mature Secondary Structure
MKEKVSMVSLGCPKNLVDAEVMLGCLAKDQYEITTDEHEADIIIVNTCSFIGDAKKESID
CCCCCCEEECCCCCCHHHHHHHHHHHCCCCEEECCCCCCCCEEEEECCHHHCCHHHHHHH
TILDLADRKHDGRCKLLVVTGCLPQRYQEELATELPEVDIFVGTADYPRIAEIIAEKRGV
HHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCH
AEQLRYISDPNYIFDESLPRLKSSPAYTAYLKIAEGCSNCCSYCVIPSLRGALRSRPLDN
HHHHHHCCCCCEECCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
LLMEARTLVATGTREINLIAQDITAWGRDLPGSPTLEELVRELAKIVGLRWIRLLYAYPD
HHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
GITDGLIEIIKTEPKVCKYLDIPIQHISDPVLKRMNRRSTEPQIRALIAKIRAEIPEIAL
CHHHHHHHHHHCCCHHHHHCCCCHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
RTSLIVGFPGETEEDFRTLLHYVEETQFDRLGVFCYSREEGTPAAGMEDQVSERVKRDRY
HHEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHH
KKLMKVQARLSFKRNRRLIDTEEEVIVEGYSDETDLLLKGRSSRQAPDIDGQVYITAGNA
HHHHHHHHHHHHHHCCCCCCCCHHHEEECCCCCCCEEEECCCCCCCCCCCCEEEEEECCC
NVGDIVRLRITDSSDYDLIGEIVEE
CCCCEEEEEEECCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA