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The map label for this gene is rlmH

Identifier: 78224393

GI number: 78224393

Start: 3602203

End: 3602667

Strand: Direct

Name: rlmH

Synonym: Gmet_3202

Alternate gene names: 78224393

Gene position: 3602203-3602667 (Clockwise)

Preceding gene: 78224392

Following gene: 78224394

Centisome position: 90.11

GC content: 64.73

Gene sequence:

>465_bases
GTGAAGCTGAAGATCCTCTGGGTCGGCAAGACCCAGGAGGAGTGGCTGCGGCGCGGCATCGACGAGTACACGGGCCGGAT
CAGGCGCTACACCCCCCTGGAAATCGCCGAGGCCCGTGAGGAGAAGGGAGCCGCCGCCGAGGCCATGAGAACCCGGGAGT
GCGAGCGGCTGGAGAAACTGGTGCCGAAGAACGCCCGCCTCGTCCTGCTGGATGAGCGGGGCGACCAGCTGACATCCCCG
GAACTGGCGGCCTTCATGGGCAAGTGCCGGGACAGCGCCGTGCCTGAGATGGTTTTCGCCATTGGCGGCGCCTACGGCTT
TGCCGACAGTTTCCGGGCCCGGGCCGACAGAGTACTCGCCCTCTCCCGTCTCACCTTTACCCACCAGATGGTGCGGGTCA
TCCTCCTCGAACAGATCTACCGAGCCCATACCATCCTCAACGGCGAACCGTATCATCACTCATGA

Upstream 100 bases:

>100_bases
AGGTACGCCGCTACTACAACCTGGACGAACTCTGGTCATCGGCCCCGACGTCGGAGATTCCCGACGCGTGGCTCTGGGAA
GGGAAAGAAGGTATCCCCAC

Downstream 100 bases:

>100_bases
TATGAATTTCTATCTTCTTATGGAGGAACCATGACCCGCCCCCTCATGCTCATGATCCTCGACGGCTGGGGGATCAACCC
CAGCGCCGAGCACAACGCCG

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase; rRNA (pseudouridine-N3-)-methyltransferase rlmH

Number of amino acids: Translated: 154; Mature: 154

Protein sequence:

>154_residues
MKLKILWVGKTQEEWLRRGIDEYTGRIRRYTPLEIAEAREEKGAAAEAMRTRECERLEKLVPKNARLVLLDERGDQLTSP
ELAAFMGKCRDSAVPEMVFAIGGAYGFADSFRARADRVLALSRLTFTHQMVRVILLEQIYRAHTILNGEPYHHS

Sequences:

>Translated_154_residues
MKLKILWVGKTQEEWLRRGIDEYTGRIRRYTPLEIAEAREEKGAAAEAMRTRECERLEKLVPKNARLVLLDERGDQLTSP
ELAAFMGKCRDSAVPEMVFAIGGAYGFADSFRARADRVLALSRLTFTHQMVRVILLEQIYRAHTILNGEPYHHS
>Mature_154_residues
MKLKILWVGKTQEEWLRRGIDEYTGRIRRYTPLEIAEAREEKGAAAEAMRTRECERLEKLVPKNARLVLLDERGDQLTSP
ELAAFMGKCRDSAVPEMVFAIGGAYGFADSFRARADRVLALSRLTFTHQMVRVILLEQIYRAHTILNGEPYHHS

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family

Homologues:

Organism=Escherichia coli, GI1786855, Length=155, Percent_Identity=32.258064516129, Blast_Score=81, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMH_GEOMG (Q39QQ9)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_386140.1
- ProteinModelPortal:   Q39QQ9
- SMR:   Q39QQ9
- STRING:   Q39QQ9
- GeneID:   3741112
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_3202
- NMPDR:   fig|269799.3.peg.2436
- eggNOG:   COG1576
- HOGENOM:   HBG622807
- OMA:   TANHPYH
- PhylomeDB:   Q39QQ9
- ProtClustDB:   CLSK829160
- BioCyc:   GMET269799:GMET_3202-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00658
- InterPro:   IPR003742
- PIRSF:   PIRSF004505

Pfam domain/function: PF02590 SPOUT_MTase

EC number: NA

Molecular weight: Translated: 17690; Mature: 17690

Theoretical pI: Translated: 9.12; Mature: 9.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKILWVGKTQEEWLRRGIDEYTGRIRRYTPLEIAEAREEKGAAAEAMRTRECERLEKL
CEEEEEEECCCHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHH
VPKNARLVLLDERGDQLTSPELAAFMGKCRDSAVPEMVFAIGGAYGFADSFRARADRVLA
CCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHH
LSRLTFTHQMVRVILLEQIYRAHTILNGEPYHHS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKLKILWVGKTQEEWLRRGIDEYTGRIRRYTPLEIAEAREEKGAAAEAMRTRECERLEKL
CEEEEEEECCCHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHH
VPKNARLVLLDERGDQLTSPELAAFMGKCRDSAVPEMVFAIGGAYGFADSFRARADRVLA
CCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHH
LSRLTFTHQMVRVILLEQIYRAHTILNGEPYHHS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA