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The map label for this gene is pol [H]

Identifier: 78224378

GI number: 78224378

Start: 3587525

End: 3589759

Strand: Direct

Name: pol [H]

Synonym: Gmet_3187

Alternate gene names: 78224378

Gene position: 3587525-3589759 (Clockwise)

Preceding gene: 78224377

Following gene: 78224379

Centisome position: 89.75

GC content: 65.77

Gene sequence:

>2235_bases
ATGGCCGAGCCCCGCCTCACCGAAAGCCCCCTCCTCTCCGGCGCCGATCCCACGGAAGGGATCGTTGCCGCCGAACTGGC
GGGACGGTTCATCCGCCTCTTCATCCGCACGCCTGGCGGCGTCATCTTCAGGGACACCCCCTTCCGCCCCTTCATCCTCC
TGGAGGACCGGGCACTTTTGGGCGGCTTCCGGAGCGACGTCGAGACCCGCCCCCTGGCCGGCCCCGGCGCCTTCCGTTAC
CTGGCCCTCTTCGCCGATTGGTCCGAATGCCTCGCCGCCCGGGATTTCCTGGCGAAGCGGACCGGCCATACCCCCTCCGC
CCCCGACGCTCCCTACCTCTTTCTCTCGGACCCGGTGCACCAACACCTCCTCCTCGCCGGCACCACCTTCTTCAAAGGGC
TGGAATTCAATGCGCTGCGCCGCCTGGCCCTGGATATCGAGACCGCCTGCGCCGAGGGGTTCGAGTTCTCCAACCCGGCG
CGGGAGGAGGACCGCATCCTTTCCATCGCCGTCATGGAGGAAGGGGGCTATGAGGAGTACCTGTCGGCCCATGCACTCTC
CGAGCGGGAGATGCTGGAACGGCTCACCGCCATCATCCGGGAGCGGGATCCGGACGTAATCGAAGGGCACAACCTCTTCC
GCTTTGACCTCGAATACATCAGGGCCCGGGCGGCCCGGCACGGGGTCAGGCTCCGTTGGGGGCGCGACGGGAGCGAGCCC
CGGGTCCACCCTTCCCGGTTCGCCGTGGCGGAGCGGACCATCGACTATCCCCGCTGGAACATTTACGGCCGCTCGGTCAT
GGACACCTACTTCCTCCTTCAAATCTATGACGTCTCCAGCCGGGAACTGGAGAGCCACGGCCTCAAGCAGGCGGCTATCC
ACTTCGGCCTCGCCTCACCCGACCGGGTCTACCTGGACCGTCAGGCCATGGACGAGGTCTTCCGCAGCGACCCGGAGGCG
CTCAGGCGCTACAATCTGGACGACGTGCGGGAGACCCTTGCCCTGTCGCGGCTCCTCTCCTACCCCTGGTTCCTTCAGGC
CCGCATCTTTCCCTACTCCTACCAGACCTGCGTCATCCGGGGGAACGCCACGCGGATCAACGCTCTCTTCCTGCGGGAAT
ACCTGCGGCAGGGCGCTGCGGTCCCGAAACCTGCCGGATCCGTGGGGACCTTCGAGGGTGGGTACGCCGCCGTCTTCGAG
ACCGGCGTTCTCGGCCCCATCGTCCACTGCGACGTGGCCTCCCTCTACCCCTCCATCATGCTTGCTTACGGCCTAGACCC
CCGACGGGAGCACCTGGGGCTCTTCCTGCCGCTCCTGGCGCAGTTGCGGCAGTTTCGCCTCGCCGCCAAGGAGCGGGCAC
GGGAGGCGGCGCTCCCCCACGAGCGAGACTACTTCAACGCCCTCCAGCAGACCTTCAAGGTGCTCATCAACTCCTTCTAC
GGCTACCTGGGAACCACCATCCACACCTTCTCCGACCCGGGGGTGGCCGCCGAGGTGACCCGCCGGGGGCGAGAGCTGAT
CCAGGACATGATCGAATGGCTTCGCCGGCACGGGGCTCGTCCCGTGGAAGTGGACACCGACGGCATCTACTTCATCCCTC
CGCAGGGGATAGAAACCATCGGGCAGGAGGAGGCCCTTGTCCGGGAGCTCTCCGCAACCCTCCCGGAAGGGATTGCCGTG
GAGATGGACGGCCGGTACCGCGCCATCTTCTCCTACAAGATAAAGAACTACGCCCTCCTGGACCACGACGGGAAGGTAAT
TGTCAGGGGAAGCGCCCTCCGCTCCCGGGGGATAGAACGGTACCTGCGGGACTTCATGGCAGCGGCGGTCCGGCTCCTGC
TACAGGGCGACGGGGCAAAAATCCCGGCGTTCCACGAGGACTGCATCCGGCGCCTGCGGAACCACCTCATCCCCATCGAG
AGCCTCGCCCGCACCGAAACCCTCGGTGAAGCGCCGCCCACCTATCTCCAGAAGGTCCGCCAAGGGAAGCGGAACCCGTC
GGCGGCATTCGAGATAGCCCTGCGCGCCGAGCGCCCCTTCCGGGCCGGCGACCAGATCAGCTACTACGTAACGGGACACG
GAAAAGGGGTGACCGTCTACGAAAACTGCAAGCCGGTCTCCCAGTACGATCCAACCCGGCCCGATGAAAATACGGAATTC
TATCTCGACAAGCTCAACCAGCTCGTGAAAAGGCTTGCCGCCTACCTGCCGAAGGAGCCCACCCTCTTTGACTGA

Upstream 100 bases:

>100_bases
CGACTACCTGGCGAAGCATGCCGCAGGGTAGCTGCCTCCACGCAGCGAAATGAAAGGCACGGTCCGGCGACCGTGCCTTT
TCTGTTTCTCCGAGGATTCC

Downstream 100 bases:

>100_bases
GGCCGCTTGCCACCTGTCGGCAAGTGTGCTACAAGGGGTAAGAAACTCCCATCACATGCCGGCAAAAGGAGAATATATGC
GTTCTTTCGCATGGTTTCTT

Product: DNA polymerase B, exonuclease

Products: NA

Alternate protein names: Pfu polymerase [H]

Number of amino acids: Translated: 744; Mature: 743

Protein sequence:

>744_residues
MAEPRLTESPLLSGADPTEGIVAAELAGRFIRLFIRTPGGVIFRDTPFRPFILLEDRALLGGFRSDVETRPLAGPGAFRY
LALFADWSECLAARDFLAKRTGHTPSAPDAPYLFLSDPVHQHLLLAGTTFFKGLEFNALRRLALDIETACAEGFEFSNPA
REEDRILSIAVMEEGGYEEYLSAHALSEREMLERLTAIIRERDPDVIEGHNLFRFDLEYIRARAARHGVRLRWGRDGSEP
RVHPSRFAVAERTIDYPRWNIYGRSVMDTYFLLQIYDVSSRELESHGLKQAAIHFGLASPDRVYLDRQAMDEVFRSDPEA
LRRYNLDDVRETLALSRLLSYPWFLQARIFPYSYQTCVIRGNATRINALFLREYLRQGAAVPKPAGSVGTFEGGYAAVFE
TGVLGPIVHCDVASLYPSIMLAYGLDPRREHLGLFLPLLAQLRQFRLAAKERAREAALPHERDYFNALQQTFKVLINSFY
GYLGTTIHTFSDPGVAAEVTRRGRELIQDMIEWLRRHGARPVEVDTDGIYFIPPQGIETIGQEEALVRELSATLPEGIAV
EMDGRYRAIFSYKIKNYALLDHDGKVIVRGSALRSRGIERYLRDFMAAAVRLLLQGDGAKIPAFHEDCIRRLRNHLIPIE
SLARTETLGEAPPTYLQKVRQGKRNPSAAFEIALRAERPFRAGDQISYYVTGHGKGVTVYENCKPVSQYDPTRPDENTEF
YLDKLNQLVKRLAAYLPKEPTLFD

Sequences:

>Translated_744_residues
MAEPRLTESPLLSGADPTEGIVAAELAGRFIRLFIRTPGGVIFRDTPFRPFILLEDRALLGGFRSDVETRPLAGPGAFRY
LALFADWSECLAARDFLAKRTGHTPSAPDAPYLFLSDPVHQHLLLAGTTFFKGLEFNALRRLALDIETACAEGFEFSNPA
REEDRILSIAVMEEGGYEEYLSAHALSEREMLERLTAIIRERDPDVIEGHNLFRFDLEYIRARAARHGVRLRWGRDGSEP
RVHPSRFAVAERTIDYPRWNIYGRSVMDTYFLLQIYDVSSRELESHGLKQAAIHFGLASPDRVYLDRQAMDEVFRSDPEA
LRRYNLDDVRETLALSRLLSYPWFLQARIFPYSYQTCVIRGNATRINALFLREYLRQGAAVPKPAGSVGTFEGGYAAVFE
TGVLGPIVHCDVASLYPSIMLAYGLDPRREHLGLFLPLLAQLRQFRLAAKERAREAALPHERDYFNALQQTFKVLINSFY
GYLGTTIHTFSDPGVAAEVTRRGRELIQDMIEWLRRHGARPVEVDTDGIYFIPPQGIETIGQEEALVRELSATLPEGIAV
EMDGRYRAIFSYKIKNYALLDHDGKVIVRGSALRSRGIERYLRDFMAAAVRLLLQGDGAKIPAFHEDCIRRLRNHLIPIE
SLARTETLGEAPPTYLQKVRQGKRNPSAAFEIALRAERPFRAGDQISYYVTGHGKGVTVYENCKPVSQYDPTRPDENTEF
YLDKLNQLVKRLAAYLPKEPTLFD
>Mature_743_residues
AEPRLTESPLLSGADPTEGIVAAELAGRFIRLFIRTPGGVIFRDTPFRPFILLEDRALLGGFRSDVETRPLAGPGAFRYL
ALFADWSECLAARDFLAKRTGHTPSAPDAPYLFLSDPVHQHLLLAGTTFFKGLEFNALRRLALDIETACAEGFEFSNPAR
EEDRILSIAVMEEGGYEEYLSAHALSEREMLERLTAIIRERDPDVIEGHNLFRFDLEYIRARAARHGVRLRWGRDGSEPR
VHPSRFAVAERTIDYPRWNIYGRSVMDTYFLLQIYDVSSRELESHGLKQAAIHFGLASPDRVYLDRQAMDEVFRSDPEAL
RRYNLDDVRETLALSRLLSYPWFLQARIFPYSYQTCVIRGNATRINALFLREYLRQGAAVPKPAGSVGTFEGGYAAVFET
GVLGPIVHCDVASLYPSIMLAYGLDPRREHLGLFLPLLAQLRQFRLAAKERAREAALPHERDYFNALQQTFKVLINSFYG
YLGTTIHTFSDPGVAAEVTRRGRELIQDMIEWLRRHGARPVEVDTDGIYFIPPQGIETIGQEEALVRELSATLPEGIAVE
MDGRYRAIFSYKIKNYALLDHDGKVIVRGSALRSRGIERYLRDFMAAAVRLLLQGDGAKIPAFHEDCIRRLRNHLIPIES
LARTETLGEAPPTYLQKVRQGKRNPSAAFEIALRAERPFRAGDQISYYVTGHGKGVTVYENCKPVSQYDPTRPDENTEFY
LDKLNQLVKRLAAYLPKEPTLFD

Specific function: In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' exonuclease activity [H]

COG id: COG0417

COG function: function code L; DNA polymerase elongation subunit (family B)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA polymerase type-B family [H]

Homologues:

Organism=Escherichia coli, GI1786246, Length=374, Percent_Identity=27.807486631016, Blast_Score=97, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24664937, Length=701, Percent_Identity=24.1084165477889, Blast_Score=99, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006172
- InterPro:   IPR017964
- InterPro:   IPR006133
- InterPro:   IPR006134
- InterPro:   IPR004578
- InterPro:   IPR023211
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00136 DNA_pol_B; PF03104 DNA_pol_B_exo [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 84356; Mature: 84225

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEPRLTESPLLSGADPTEGIVAAELAGRFIRLFIRTPGGVIFRDTPFRPFILLEDRALL
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHEEECCCCEEEECCCCCCEEEECCHHHH
GGFRSDVETRPLAGPGAFRYLALFADWSECLAARDFLAKRTGHTPSAPDAPYLFLSDPVH
CHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCHHH
QHLLLAGTTFFKGLEFNALRRLALDIETACAEGFEFSNPAREEDRILSIAVMEEGGYEEY
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCHHHH
LSAHALSEREMLERLTAIIRERDPDVIEGHNLFRFDLEYIRARAARHGVRLRWGRDGSEP
HHHHHCCHHHHHHHHHHHHHCCCCCEECCCCEEEHHHHHHHHHHHHCCCEEEECCCCCCC
RVHPSRFAVAERTIDYPRWNIYGRSVMDTYFLLQIYDVSSRELESHGLKQAAIHFGLASP
CCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHEECCCHHHHHHCCHHHHHHHHCCCCC
DRVYLDRQAMDEVFRSDPEALRRYNLDDVRETLALSRLLSYPWFLQARIFPYSYQTCVIR
CCEEECHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEEECCEEEEEEEEE
GNATRINALFLREYLRQGAAVPKPAGSVGTFEGGYAAVFETGVLGPIVHCDVASLYPSIM
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHH
LAYGLDPRREHLGLFLPLLAQLRQFRLAAKERAREAALPHERDYFNALQQTFKVLINSFY
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
GYLGTTIHTFSDPGVAAEVTRRGRELIQDMIEWLRRHGARPVEVDTDGIYFIPPQGIETI
HHHCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEECCCCHHHC
GQEEALVRELSATLPEGIAVEMDGRYRAIFSYKIKNYALLDHDGKVIVRGSALRSRGIER
CHHHHHHHHHHHHCCCCEEEEECCCEEEEEEEEECCEEEEECCCCEEEECHHHHHHHHHH
YLRDFMAAAVRLLLQGDGAKIPAFHEDCIRRLRNHLIPIESLARTETLGEAPPTYLQKVR
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHH
QGKRNPSAAFEIALRAERPFRAGDQISYYVTGHGKGVTVYENCKPVSQYDPTRPDENTEF
CCCCCCCCCEEEEEECCCCCCCCCCEEEEEEECCCEEEEECCCCCCCCCCCCCCCCCHHH
YLDKLNQLVKRLAAYLPKEPTLFD
HHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
AEPRLTESPLLSGADPTEGIVAAELAGRFIRLFIRTPGGVIFRDTPFRPFILLEDRALL
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHEEECCCCEEEECCCCCCEEEECCHHHH
GGFRSDVETRPLAGPGAFRYLALFADWSECLAARDFLAKRTGHTPSAPDAPYLFLSDPVH
CHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCHHH
QHLLLAGTTFFKGLEFNALRRLALDIETACAEGFEFSNPAREEDRILSIAVMEEGGYEEY
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCHHHH
LSAHALSEREMLERLTAIIRERDPDVIEGHNLFRFDLEYIRARAARHGVRLRWGRDGSEP
HHHHHCCHHHHHHHHHHHHHCCCCCEECCCCEEEHHHHHHHHHHHHCCCEEEECCCCCCC
RVHPSRFAVAERTIDYPRWNIYGRSVMDTYFLLQIYDVSSRELESHGLKQAAIHFGLASP
CCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHEECCCHHHHHHCCHHHHHHHHCCCCC
DRVYLDRQAMDEVFRSDPEALRRYNLDDVRETLALSRLLSYPWFLQARIFPYSYQTCVIR
CCEEECHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEEECCEEEEEEEEE
GNATRINALFLREYLRQGAAVPKPAGSVGTFEGGYAAVFETGVLGPIVHCDVASLYPSIM
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHH
LAYGLDPRREHLGLFLPLLAQLRQFRLAAKERAREAALPHERDYFNALQQTFKVLINSFY
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
GYLGTTIHTFSDPGVAAEVTRRGRELIQDMIEWLRRHGARPVEVDTDGIYFIPPQGIETI
HHHCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEECCCCHHHC
GQEEALVRELSATLPEGIAVEMDGRYRAIFSYKIKNYALLDHDGKVIVRGSALRSRGIER
CHHHHHHHHHHHHCCCCEEEEECCCEEEEEEEEECCEEEEECCCCEEEECHHHHHHHHHH
YLRDFMAAAVRLLLQGDGAKIPAFHEDCIRRLRNHLIPIESLARTETLGEAPPTYLQKVR
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHH
QGKRNPSAAFEIALRAERPFRAGDQISYYVTGHGKGVTVYENCKPVSQYDPTRPDENTEF
CCCCCCCCCEEEEEECCCCCCCCCCEEEEEEECCCEEEEECCCCCCCCCCCCCCCCCHHH
YLDKLNQLVKRLAAYLPKEPTLFD
HHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8441634; 1762925; 1579479 [H]