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The map label for this gene is pcnB [H]

Identifier: 78224373

GI number: 78224373

Start: 3582804

End: 3584177

Strand: Reverse

Name: pcnB [H]

Synonym: Gmet_3182

Alternate gene names: 78224373

Gene position: 3584177-3582804 (Counterclockwise)

Preceding gene: 78224395

Following gene: 78224372

Centisome position: 89.66

GC content: 66.38

Gene sequence:

>1374_bases
ATGAAAACCCCTATGAACAGCGAACCAGTCATCATCCCCCGCCCCGATCATCCCATCTCCCGCTCCCAAGTGAGCCCCAA
CGCACTCCGGGTTCTCTACCGTCTCAAGGATAACGGCTGCATTGCCTACCTCGTGGGAGGATGCGTCCGCGACCTCCTGC
TGGGACGCGAACCCAAGGATTTCGATGTGGCCACCAACGCCACCCCAAACCAGGTGAAGCGGCTGTTCCGCAACTGCCGT
CTGGTGGGGCGCAGGTTCCGGCTCGCCCACATCCATTACCAGGACGAGATTATCGAGGTTGCCACTTTCCGGGCCCTGGC
GCCGGAGGAGCCCGAAGATACCGCGCCTGCTCCGGTCTCCCCGGAGAACGTGCCCGGCGAGGGGGAGCGCCCCCGGCTGC
CCCGCCATGTGGTGAGCGACGAGGGAATGGTGCTGCGGGACAACGTCTTCGGGACCCCTGAAGAGGACGCCGTGCGGCGG
GACTTCACCGTGAACGCCCTGGCTTACAACATCGCGGATTTTTCCATCATCGACTATGCGAATGGCATGGAAGACCTGCG
GCGGGGGGTGATCCGGACCATCGGCGATCCCCTTGTCCGCTTCACGGAAGACCCCGTCCGGATGCTGAGGGCGGTGCGCT
TTGCCGCCACCCTGGGTTTCACCGTGGAGGATGCCACCTGGCAGGCAATCCGGGAGCTGGCCCCCGCCATCACCCGGGCC
ACGGCCCCCCGACTCTACGAGGAGGTGCTGAAGCTCTTCCTCTCGGGTGAAGGGGAGCGGGTCTACCAGCTGCTCCGCCA
GGCAGGGCTTTTCAGTCATCTCTTTCCCCGTTTCAGCGCCTGGCTCGACCGGGAGATCGACGGTTTTCCCCATTCCCAGG
TCGGCAAGGCCCTGGAGTGGGCGGACGATCAGGTGGGGGGCGGAGAGCCGGTCTCGCCCCCCCTCCTTCTCGCCCTCATG
TTCGGCGAGTACGTGGAGGAACGGGCCGCGGAGTTGGCTGATGCGGGGATGCCGGTGCCGGACGCGGTCAACGCGGCAAT
GGCCGGCTTTCTTGGCGAGCAGGCGCCGATCATTGCCATTCCCCAGCGGATCGCGCTCGCTGTGCGGGAGATTCTGCTGA
TTCAGCACCGTCTCCGGAAGATACCGGGGAAGCGGCCTCAGGGGGTACTTGCCCGATCCTGCTTTGCCGAGGCCCTGGCC
TACCTCCGTTGCCGGTGCGCGGTGACGGGGGAGGGTGGGAAAATTCTCGCCTGGTGGGAGCGCTACGCCCGGGAAAGCGT
CATGCCGCCGGTGGAGCCGGCGACCGGCGGAGGAGAAGCTCCCCCGAAACGCCGGCGGCGCCGGGGGAAAAGGCGGAAGC
CTCCTGCCGCGTGA

Upstream 100 bases:

>100_bases
CGAAAAAAGAACAATTGTGCCAAAGAGCAATAAAGTTCAACAAGTTACAGCTGTACAATCAAAGTTTACCGGACAGTAGT
ATTTGTCCCCGTTGTGTGCT

Downstream 100 bases:

>100_bases
GTGTCGACGGGATTGCTACCTGTCCGCGGAGAGCCTCTCCTTCCATCGCCGATAGGCGAAGATCCGTTCGATGAAGCTTT
TCATAAGCTCCCGGTCGCGG

Product: Poly(A) polymerase, PcnB

Products: NA

Alternate protein names: PAP; Plasmid copy number protein [H]

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MKTPMNSEPVIIPRPDHPISRSQVSPNALRVLYRLKDNGCIAYLVGGCVRDLLLGREPKDFDVATNATPNQVKRLFRNCR
LVGRRFRLAHIHYQDEIIEVATFRALAPEEPEDTAPAPVSPENVPGEGERPRLPRHVVSDEGMVLRDNVFGTPEEDAVRR
DFTVNALAYNIADFSIIDYANGMEDLRRGVIRTIGDPLVRFTEDPVRMLRAVRFAATLGFTVEDATWQAIRELAPAITRA
TAPRLYEEVLKLFLSGEGERVYQLLRQAGLFSHLFPRFSAWLDREIDGFPHSQVGKALEWADDQVGGGEPVSPPLLLALM
FGEYVEERAAELADAGMPVPDAVNAAMAGFLGEQAPIIAIPQRIALAVREILLIQHRLRKIPGKRPQGVLARSCFAEALA
YLRCRCAVTGEGGKILAWWERYARESVMPPVEPATGGGEAPPKRRRRRGKRRKPPAA

Sequences:

>Translated_457_residues
MKTPMNSEPVIIPRPDHPISRSQVSPNALRVLYRLKDNGCIAYLVGGCVRDLLLGREPKDFDVATNATPNQVKRLFRNCR
LVGRRFRLAHIHYQDEIIEVATFRALAPEEPEDTAPAPVSPENVPGEGERPRLPRHVVSDEGMVLRDNVFGTPEEDAVRR
DFTVNALAYNIADFSIIDYANGMEDLRRGVIRTIGDPLVRFTEDPVRMLRAVRFAATLGFTVEDATWQAIRELAPAITRA
TAPRLYEEVLKLFLSGEGERVYQLLRQAGLFSHLFPRFSAWLDREIDGFPHSQVGKALEWADDQVGGGEPVSPPLLLALM
FGEYVEERAAELADAGMPVPDAVNAAMAGFLGEQAPIIAIPQRIALAVREILLIQHRLRKIPGKRPQGVLARSCFAEALA
YLRCRCAVTGEGGKILAWWERYARESVMPPVEPATGGGEAPPKRRRRRGKRRKPPAA
>Mature_457_residues
MKTPMNSEPVIIPRPDHPISRSQVSPNALRVLYRLKDNGCIAYLVGGCVRDLLLGREPKDFDVATNATPNQVKRLFRNCR
LVGRRFRLAHIHYQDEIIEVATFRALAPEEPEDTAPAPVSPENVPGEGERPRLPRHVVSDEGMVLRDNVFGTPEEDAVRR
DFTVNALAYNIADFSIIDYANGMEDLRRGVIRTIGDPLVRFTEDPVRMLRAVRFAATLGFTVEDATWQAIRELAPAITRA
TAPRLYEEVLKLFLSGEGERVYQLLRQAGLFSHLFPRFSAWLDREIDGFPHSQVGKALEWADDQVGGGEPVSPPLLLALM
FGEYVEERAAELADAGMPVPDAVNAAMAGFLGEQAPIIAIPQRIALAVREILLIQHRLRKIPGKRPQGVLARSCFAEALA
YLRCRCAVTGEGGKILAWWERYARESVMPPVEPATGGGEAPPKRRRRRGKRRKPPAA

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's. Seems to be involved in plasmid copy number control [H]

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family [H]

Homologues:

Organism=Escherichia coli, GI87081691, Length=413, Percent_Identity=44.0677966101695, Blast_Score=319, Evalue=2e-88,
Organism=Escherichia coli, GI1789436, Length=244, Percent_Identity=32.7868852459016, Blast_Score=81, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6321016, Length=193, Percent_Identity=33.160621761658, Blast_Score=81, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002646
- InterPro:   IPR010206 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.19 [H]

Molecular weight: Translated: 50894; Mature: 50894

Theoretical pI: Translated: 8.56; Mature: 8.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTPMNSEPVIIPRPDHPISRSQVSPNALRVLYRLKDNGCIAYLVGGCVRDLLLGREPKD
CCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHCCCCCEEHHHHHHHHHHHHHCCCCCC
FDVATNATPNQVKRLFRNCRLVGRRFRLAHIHYQDEIIEVATFRALAPEEPEDTAPAPVS
CCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCC
PENVPGEGERPRLPRHVVSDEGMVLRDNVFGTPEEDAVRRDFTVNALAYNIADFSIIDYA
CCCCCCCCCCCCCCHHHHCCCCEEEECCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHH
NGMEDLRRGVIRTIGDPLVRFTEDPVRMLRAVRFAATLGFTVEDATWQAIRELAPAITRA
CCHHHHHHHHHHHHCCHHHHHCHHHHHHHHHHHHHHHHCCEECHHHHHHHHHHHHHHHHH
TAPRLYEEVLKLFLSGEGERVYQLLRQAGLFSHLFPRFSAWLDREIDGFPHSQVGKALEW
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
ADDQVGGGEPVSPPLLLALMFGEYVEERAAELADAGMPVPDAVNAAMAGFLGEQAPIIAI
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEC
PQRIALAVREILLIQHRLRKIPGKRPQGVLARSCFAEALAYLRCRCAVTGEGGKILAWWE
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHH
RYARESVMPPVEPATGGGEAPPKRRRRRGKRRKPPAA
HHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKTPMNSEPVIIPRPDHPISRSQVSPNALRVLYRLKDNGCIAYLVGGCVRDLLLGREPKD
CCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHCCCCCEEHHHHHHHHHHHHHCCCCCC
FDVATNATPNQVKRLFRNCRLVGRRFRLAHIHYQDEIIEVATFRALAPEEPEDTAPAPVS
CCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCC
PENVPGEGERPRLPRHVVSDEGMVLRDNVFGTPEEDAVRRDFTVNALAYNIADFSIIDYA
CCCCCCCCCCCCCCHHHHCCCCEEEECCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHH
NGMEDLRRGVIRTIGDPLVRFTEDPVRMLRAVRFAATLGFTVEDATWQAIRELAPAITRA
CCHHHHHHHHHHHHCCHHHHHCHHHHHHHHHHHHHHHHCCEECHHHHHHHHHHHHHHHHH
TAPRLYEEVLKLFLSGEGERVYQLLRQAGLFSHLFPRFSAWLDREIDGFPHSQVGKALEW
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
ADDQVGGGEPVSPPLLLALMFGEYVEERAAELADAGMPVPDAVNAAMAGFLGEQAPIIAI
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEC
PQRIALAVREILLIQHRLRKIPGKRPQGVLARSCFAEALAYLRCRCAVTGEGGKILAWWE
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHH
RYARESVMPPVEPATGGGEAPPKRRRRRGKRRKPPAA
HHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]