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The map label for this gene is kinE [H]

Identifier: 78224371

GI number: 78224371

Start: 3580680

End: 3582302

Strand: Reverse

Name: kinE [H]

Synonym: Gmet_3180

Alternate gene names: 78224371

Gene position: 3582302-3580680 (Counterclockwise)

Preceding gene: 78224372

Following gene: 78224370

Centisome position: 89.62

GC content: 60.2

Gene sequence:

>1623_bases
GTGGATTGGGTCTGTTGTTGGCACAGAAAAGTGGTTGAACCGGGAGGATGTCCGAATATCGGCGAGGGGGAGGAGGTTCT
CTACACCTCCTACCAGCGGCGGATCCTTGAAAAGTGCATCGACTGTCCCAATTTCCGTAATGACCTGCAGAAGATGCAGG
AGTCGGGAAATCCCCTGGGAGAAGTGGTTTCGTTCCTGCTCGGCGAAGTCAGGGAACAGCGGGGAAAGCTCCAGTCCATG
GTCAGTTTTCTCGACAGCCGGGAACGGGAGATTAAGTTCCTCCACGAGATCAGCCTCGTTCTCCAGACCTCCGTCGACCT
GGACGAAGTCCTTTCGGTGGTAATGACCGCCATCACCGCCGGCAAGGGGTTCGGCATGAACCGGGCCTTCCTCCTGCTTG
CCGACAAAGAGCGGAAATTTCTCCGGGGGTACCTGGGTGTGGGCCCCCTGACCTTCGGCGAGGCGTGGGAGATATGGGAG
GATATCCAGCGGGACGACTTTACCCTCAAGGAGATGGCCAGGGACTTCTACCAGACCAAGCTCACCTCCGAGAAGCAGAA
GTTCCACGACGTCCTCAAGCGGATGACCATCCCCCTGTCGGACCCGAACCATGTTCTCGTCCGGGCGTTGCGGGAAAAGA
GGCCGATCCTGGTGGAGAACGCCTTCCAGAACCCCGAATGTGATCACTCCCTGGTTCAGGCCCTGGGGGTGGATACCTTC
CTCATCACGCCGCTCATCTCCCGCAACCGCCGGATCGGGGTGATCATCTCCGACAATTGCATCACCCACAAGCAGATCAC
CCCCCAGGACGTGCAATCCCTGGAGACCTTCGCCTTCTCCGTGGCCTTCGCCATCGAACGTGCCTCGCTCTACGAGCGGC
TCCAGGAGGAGCTCCTCAAGGTGACGGCCGCCAACGTGAAGCTCAAGGAGCAGCAGGAGCTGATCGTCAGGATGGAGCGG
ATGGCGCTCGTGGGGAAGATCACCTCCAGCATCGCCCACTCCATCCGCAATCCCCTCATGATCATCGGCGGGTTTGCCCG
CACGCTCCTGCGGGGGGCTGCCGATGACGACCCCCAGCGGGAGTACCTGGAGTCCATTGGCCAGCAGGTGCGCCAGTTGG
AGGAAGTTCTGGAGGATATTCTCAACTACTCCGATTCCCTCTACCCGACCTTGGATATCTGGGACGTGAACCAACTGGTG
AGTGCTGTCTGCCGCGAGTTGAACGGCCGGCTCCACGAGCGGCGGGTGGCTTGCCGCCTCCGGCTCGACACAGGCCTGCC
GCCGGCCCGCATTGATTACAAGCAGGTGGCTTACTGTGTCAGAACCATCGTCAATACGGCCATGGAAGGTATGCCGGAGG
GGGGGGAGATCAGGGCCGAAACCCGTCGGGAAAACGGCTGGATCCTTGTGGAAATATCGGACAACGGCGTCGCCTTGAGC
CGGGAAAGCATCGATGCCATGACAACCCCCTTCGGCTCCACCGAGGAACTCGGTTCCGGCCTCGGCGTGGCCATCTGCAA
GACGATTATGGAGAAGCACGGCAACCTCTTCGAGGTTGATACCCCGCCTGGCGGGGGAACCCGGTATATCATCAAACTAC
CCGTGAACAGGGAGGAGACGTAA

Upstream 100 bases:

>100_bases
ATTAATTAATAAAACCTTTACCGTCTTGAAAAGGTGCTGTCTGCTGGTATAATTGTGCTGTTGTGTCGCGCATAAAATCA
ACCCTCTCGGGAGATGGTCA

Downstream 100 bases:

>100_bases
CATGGCACGACTGCTGGTGGTGGACGATGAAAGCAGCATCAGGCTGCTCTATTCCCAGGAACTGGCTGACGATGGGCACG
AAGTGGCCACCGCCGCCACT

Product: GAF sensor signal transduction histidine kinase

Products: NA

Alternate protein names: Sensor histidine kinase E [H]

Number of amino acids: Translated: 540; Mature: 540

Protein sequence:

>540_residues
MDWVCCWHRKVVEPGGCPNIGEGEEVLYTSYQRRILEKCIDCPNFRNDLQKMQESGNPLGEVVSFLLGEVREQRGKLQSM
VSFLDSREREIKFLHEISLVLQTSVDLDEVLSVVMTAITAGKGFGMNRAFLLLADKERKFLRGYLGVGPLTFGEAWEIWE
DIQRDDFTLKEMARDFYQTKLTSEKQKFHDVLKRMTIPLSDPNHVLVRALREKRPILVENAFQNPECDHSLVQALGVDTF
LITPLISRNRRIGVIISDNCITHKQITPQDVQSLETFAFSVAFAIERASLYERLQEELLKVTAANVKLKEQQELIVRMER
MALVGKITSSIAHSIRNPLMIIGGFARTLLRGAADDDPQREYLESIGQQVRQLEEVLEDILNYSDSLYPTLDIWDVNQLV
SAVCRELNGRLHERRVACRLRLDTGLPPARIDYKQVAYCVRTIVNTAMEGMPEGGEIRAETRRENGWILVEISDNGVALS
RESIDAMTTPFGSTEELGSGLGVAICKTIMEKHGNLFEVDTPPGGGTRYIIKLPVNREET

Sequences:

>Translated_540_residues
MDWVCCWHRKVVEPGGCPNIGEGEEVLYTSYQRRILEKCIDCPNFRNDLQKMQESGNPLGEVVSFLLGEVREQRGKLQSM
VSFLDSREREIKFLHEISLVLQTSVDLDEVLSVVMTAITAGKGFGMNRAFLLLADKERKFLRGYLGVGPLTFGEAWEIWE
DIQRDDFTLKEMARDFYQTKLTSEKQKFHDVLKRMTIPLSDPNHVLVRALREKRPILVENAFQNPECDHSLVQALGVDTF
LITPLISRNRRIGVIISDNCITHKQITPQDVQSLETFAFSVAFAIERASLYERLQEELLKVTAANVKLKEQQELIVRMER
MALVGKITSSIAHSIRNPLMIIGGFARTLLRGAADDDPQREYLESIGQQVRQLEEVLEDILNYSDSLYPTLDIWDVNQLV
SAVCRELNGRLHERRVACRLRLDTGLPPARIDYKQVAYCVRTIVNTAMEGMPEGGEIRAETRRENGWILVEISDNGVALS
RESIDAMTTPFGSTEELGSGLGVAICKTIMEKHGNLFEVDTPPGGGTRYIIKLPVNREET
>Mature_540_residues
MDWVCCWHRKVVEPGGCPNIGEGEEVLYTSYQRRILEKCIDCPNFRNDLQKMQESGNPLGEVVSFLLGEVREQRGKLQSM
VSFLDSREREIKFLHEISLVLQTSVDLDEVLSVVMTAITAGKGFGMNRAFLLLADKERKFLRGYLGVGPLTFGEAWEIWE
DIQRDDFTLKEMARDFYQTKLTSEKQKFHDVLKRMTIPLSDPNHVLVRALREKRPILVENAFQNPECDHSLVQALGVDTF
LITPLISRNRRIGVIISDNCITHKQITPQDVQSLETFAFSVAFAIERASLYERLQEELLKVTAANVKLKEQQELIVRMER
MALVGKITSSIAHSIRNPLMIIGGFARTLLRGAADDDPQREYLESIGQQVRQLEEVLEDILNYSDSLYPTLDIWDVNQLV
SAVCRELNGRLHERRVACRLRLDTGLPPARIDYKQVAYCVRTIVNTAMEGMPEGGEIRAETRRENGWILVEISDNGVALS
RESIDAMTTPFGSTEELGSGLGVAICKTIMEKHGNLFEVDTPPGGGTRYIIKLPVNREET

Specific function: Phosphorylates the sporulation-regulatory protein spo0A under biofilm growth conditions. Also able to weakly phosphorylate spo0F [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=225, Percent_Identity=23.5555555555556, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1788549, Length=229, Percent_Identity=26.6375545851528, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1790300, Length=255, Percent_Identity=25.8823529411765, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1786600, Length=216, Percent_Identity=23.6111111111111, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 61216; Mature: 61216

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDWVCCWHRKVVEPGGCPNIGEGEEVLYTSYQRRILEKCIDCPNFRNDLQKMQESGNPLG
CCCEEECHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHH
EVVSFLLGEVREQRGKLQSMVSFLDSREREIKFLHEISLVLQTSVDLDEVLSVVMTAITA
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHC
GKGFGMNRAFLLLADKERKFLRGYLGVGPLTFGEAWEIWEDIQRDDFTLKEMARDFYQTK
CCCCCCCCEEEEEECCHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHH
LTSEKQKFHDVLKRMTIPLSDPNHVLVRALREKRPILVENAFQNPECDHSLVQALGVDTF
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHCCCHH
LITPLISRNRRIGVIISDNCITHKQITPQDVQSLETFAFSVAFAIERASLYERLQEELLK
HHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VTAANVKLKEQQELIVRMERMALVGKITSSIAHSIRNPLMIIGGFARTLLRGAADDDPQR
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHCCCCCCHHH
EYLESIGQQVRQLEEVLEDILNYSDSLYPTLDIWDVNQLVSAVCRELNGRLHERRVACRL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCHHHHHHHHHHHHHCHHHHHHHHHEE
RLDTGLPPARIDYKQVAYCVRTIVNTAMEGMPEGGEIRAETRRENGWILVEISDNGVALS
EECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEEECCCCEEEE
RESIDAMTTPFGSTEELGSGLGVAICKTIMEKHGNLFEVDTPPGGGTRYIIKLPVNREET
HHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHCCCEEEECCCCCCCEEEEEEECCCCCCC
>Mature Secondary Structure
MDWVCCWHRKVVEPGGCPNIGEGEEVLYTSYQRRILEKCIDCPNFRNDLQKMQESGNPLG
CCCEEECHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHH
EVVSFLLGEVREQRGKLQSMVSFLDSREREIKFLHEISLVLQTSVDLDEVLSVVMTAITA
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHC
GKGFGMNRAFLLLADKERKFLRGYLGVGPLTFGEAWEIWEDIQRDDFTLKEMARDFYQTK
CCCCCCCCEEEEEECCHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHH
LTSEKQKFHDVLKRMTIPLSDPNHVLVRALREKRPILVENAFQNPECDHSLVQALGVDTF
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHCCCHH
LITPLISRNRRIGVIISDNCITHKQITPQDVQSLETFAFSVAFAIERASLYERLQEELLK
HHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VTAANVKLKEQQELIVRMERMALVGKITSSIAHSIRNPLMIIGGFARTLLRGAADDDPQR
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHCCCCCCHHH
EYLESIGQQVRQLEEVLEDILNYSDSLYPTLDIWDVNQLVSAVCRELNGRLHERRVACRL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCHHHHHHHHHHHHHCHHHHHHHHHEE
RLDTGLPPARIDYKQVAYCVRTIVNTAMEGMPEGGEIRAETRRENGWILVEISDNGVALS
EECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEEECCCCEEEE
RESIDAMTTPFGSTEELGSGLGVAICKTIMEKHGNLFEVDTPPGGGTRYIIKLPVNREET
HHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHCCCEEEECCCCCCCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]