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The map label for this gene is pckA

Identifier: 78224360

GI number: 78224360

Start: 3567233

End: 3568825

Strand: Reverse

Name: pckA

Synonym: Gmet_3169

Alternate gene names: 78224360

Gene position: 3568825-3567233 (Counterclockwise)

Preceding gene: 78224363

Following gene: 78224359

Centisome position: 89.28

GC content: 61.77

Gene sequence:

>1593_bases
GTGAGACTGAACGACATAACCAGAGGCACCGGGCTCGAGGAGCATGGGATTACCAACGCGAACCTCATCTACTGGACCCC
CCCGACCGCCGTGCTCTACGAGCAGATCGTGAAGCGGGGCGAGGGGCTCGTGACCCATCTGGGCGCCGTTGCGGTGAAGA
CCGGCCATTACACCGGCCGGGCAGCCAACGAGAAGTTCATTGTCGATGAGCCCTCCAGCAACGACCACATCGCCTGGGGC
AAAGTGAACCAGCCCTTCGACTCCGCCAAATTCGACGCCCTCTTCGGCCGGATGCTGGCCTATCTCCATGGCAAGGACAT
TTTCGTTCAGGAGTGCTTTGCGGGGTGCAGCCCCGACCACCGGCTTCCGGTCAGGGTCATCACTGAGCGTGCCTGGCACT
CCCTCTTCGCCCGCAACATGTTCGTGCGGGCAACCCCCGAGGAGTTGGTCGGCTTCAAGCCGGGTTTCACGGTCATCGAC
CTCCCCGCTTTCCACGCAATCCCCTCCGTGGACGGCACCAACACCGAGACCTTCATCATCGTCAACTTCGAAAAGAGGCT
CATCATCATCGGCGGCACCAGCTATGCCGGCGAGATCAAAAAATCCATCTTCACCATCCTCAACTACCTCCTTCCCCAGC
ACAAGAATGTCCTCTCCATGCATTGCTCCGCCAACGTGGGAGAGAAAGACGACGTGGCAGTCTTCTTCGGCCTTTCCGGC
ACCGGCAAGACCACCCTCTCCGCCGACCCGCGGCGCCGGCTCATCGGTGACGACGAGCACGGCTGGGACAACAGCGGCGT
CTTCAACTTCGAGGGGGGGTGCTATGCCAAGATCATCAACCTCTCTCCCGAGGCCGAGCCGGAGATCTACCAGACCACGC
GGCGCTTCGGAACGATACTGGAAAACGTGGCCATCGACACCGTGAGCCGGCGCATCGACCTGAACGACGACTCCTTCACC
GAGAATACCCGGGCCTCGTACCCCATCACCCACATCCCGAACATCGTGAAATCGGGGATGGGGGGACATCCGACCAATAT
CATCATGCTCACCTGCGACGCCTTCGGGGTCCTTCCCCCCATTGCCCGGCTCACCCCTGACCAGGCCATGTACCACTTCC
TCTCCGGCTACACCGCCAAGGTGGCCGGTACCGAGGCAGGGATCACCGAGCCCCAGGCGGCCTTTTCCGCCTGCTTCGGC
GCTCCCTTTATGGCGCTCCACCCGTCGGTCTACGCCAAGCTTCTCGGTGAGAAAATAGCCCGGCATGGGGTCAGCTGCTG
GCTCGTGAACACCGGCTGGAGTGGCGGCCCCTACGGCGTCGGCTCCCGCATGAAGATCGCCTACTCCCGCGCCCTGGTCA
ACGCCGCCATCGACGGGACCCTCAATGCCGGTTCCTTCGTGAAGGACCAGTTCTTCGGCCTTGACATCCCCACCGGCTGC
TCCGGTGTCCCCGCCGAGGTCCTCAATCCGAAGAACACCTGGGTCGACAAAACGAAGTACGACGAAACCGCAACTATGCT
CGTGGAGCGTTTCCGCAAGAACTTCGAGCAGTATCGCTCCTATGTTTCAGCCGGAGTGGCAGAGGTCATGTAG

Upstream 100 bases:

>100_bases
GTTTTTCTGGTTTTTACTGGTGCTTGATATTTTCTTGTGCTAGTAAAGGTGTCAGTTTCTCTATCTGGGCAAAACCTAAT
CCCCCATGGAGGTGACGACC

Downstream 100 bases:

>100_bases
GTTTTTTAGCGCGCGAGGTAACGGTGAACGAAAAAAGGCTCCCGGGGAGGGGGCCTTTTTTCTTGAGTACAAATGCAAAA
CGCACTGCACTATTGTAAAA

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase

Number of amino acids: Translated: 530; Mature: 530

Protein sequence:

>530_residues
MRLNDITRGTGLEEHGITNANLIYWTPPTAVLYEQIVKRGEGLVTHLGAVAVKTGHYTGRAANEKFIVDEPSSNDHIAWG
KVNQPFDSAKFDALFGRMLAYLHGKDIFVQECFAGCSPDHRLPVRVITERAWHSLFARNMFVRATPEELVGFKPGFTVID
LPAFHAIPSVDGTNTETFIIVNFEKRLIIIGGTSYAGEIKKSIFTILNYLLPQHKNVLSMHCSANVGEKDDVAVFFGLSG
TGKTTLSADPRRRLIGDDEHGWDNSGVFNFEGGCYAKIINLSPEAEPEIYQTTRRFGTILENVAIDTVSRRIDLNDDSFT
ENTRASYPITHIPNIVKSGMGGHPTNIIMLTCDAFGVLPPIARLTPDQAMYHFLSGYTAKVAGTEAGITEPQAAFSACFG
APFMALHPSVYAKLLGEKIARHGVSCWLVNTGWSGGPYGVGSRMKIAYSRALVNAAIDGTLNAGSFVKDQFFGLDIPTGC
SGVPAEVLNPKNTWVDKTKYDETATMLVERFRKNFEQYRSYVSAGVAEVM

Sequences:

>Translated_530_residues
MRLNDITRGTGLEEHGITNANLIYWTPPTAVLYEQIVKRGEGLVTHLGAVAVKTGHYTGRAANEKFIVDEPSSNDHIAWG
KVNQPFDSAKFDALFGRMLAYLHGKDIFVQECFAGCSPDHRLPVRVITERAWHSLFARNMFVRATPEELVGFKPGFTVID
LPAFHAIPSVDGTNTETFIIVNFEKRLIIIGGTSYAGEIKKSIFTILNYLLPQHKNVLSMHCSANVGEKDDVAVFFGLSG
TGKTTLSADPRRRLIGDDEHGWDNSGVFNFEGGCYAKIINLSPEAEPEIYQTTRRFGTILENVAIDTVSRRIDLNDDSFT
ENTRASYPITHIPNIVKSGMGGHPTNIIMLTCDAFGVLPPIARLTPDQAMYHFLSGYTAKVAGTEAGITEPQAAFSACFG
APFMALHPSVYAKLLGEKIARHGVSCWLVNTGWSGGPYGVGSRMKIAYSRALVNAAIDGTLNAGSFVKDQFFGLDIPTGC
SGVPAEVLNPKNTWVDKTKYDETATMLVERFRKNFEQYRSYVSAGVAEVM
>Mature_530_residues
MRLNDITRGTGLEEHGITNANLIYWTPPTAVLYEQIVKRGEGLVTHLGAVAVKTGHYTGRAANEKFIVDEPSSNDHIAWG
KVNQPFDSAKFDALFGRMLAYLHGKDIFVQECFAGCSPDHRLPVRVITERAWHSLFARNMFVRATPEELVGFKPGFTVID
LPAFHAIPSVDGTNTETFIIVNFEKRLIIIGGTSYAGEIKKSIFTILNYLLPQHKNVLSMHCSANVGEKDDVAVFFGLSG
TGKTTLSADPRRRLIGDDEHGWDNSGVFNFEGGCYAKIINLSPEAEPEIYQTTRRFGTILENVAIDTVSRRIDLNDDSFT
ENTRASYPITHIPNIVKSGMGGHPTNIIMLTCDAFGVLPPIARLTPDQAMYHFLSGYTAKVAGTEAGITEPQAAFSACFG
APFMALHPSVYAKLLGEKIARHGVSCWLVNTGWSGGPYGVGSRMKIAYSRALVNAAIDGTLNAGSFVKDQFFGLDIPTGC
SGVPAEVLNPKNTWVDKTKYDETATMLVERFRKNFEQYRSYVSAGVAEVM

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family

Homologues:

Organism=Escherichia coli, GI1789807, Length=542, Percent_Identity=48.8929889298893, Blast_Score=499, Evalue=1e-142,
Organism=Saccharomyces cerevisiae, GI6322950, Length=505, Percent_Identity=48.1188118811881, Blast_Score=480, Evalue=1e-136,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): PCKA_GEOMG (Q39QU2)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_386107.1
- ProteinModelPortal:   Q39QU2
- SMR:   Q39QU2
- STRING:   Q39QU2
- GeneID:   3741040
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_3169
- NMPDR:   fig|269799.3.peg.2404
- eggNOG:   COG1866
- HOGENOM:   HBG293387
- OMA:   FAQDLYG
- PhylomeDB:   Q39QU2
- ProtClustDB:   PRK09344
- BioCyc:   GMET269799:GMET_3169-MONOMER
- GO:   GO:0005737
- GO:   GO:0006094
- HAMAP:   MF_00453
- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210
- InterPro:   IPR015994
- Gene3D:   G3DSA:3.90.228.20
- Gene3D:   G3DSA:3.40.449.10
- PIRSF:   PIRSF006294
- TIGRFAMs:   TIGR00224

Pfam domain/function: PF01293 PEPCK_ATP; SSF68923 PEP_carboxykinase_N

EC number: =4.1.1.49

Molecular weight: Translated: 58151; Mature: 58151

Theoretical pI: Translated: 6.83; Mature: 6.83

Prosite motif: PS00532 PEPCK_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLNDITRGTGLEEHGITNANLIYWTPPTAVLYEQIVKRGEGLVTHLGAVAVKTGHYTGR
CCCCCCCCCCCCHHCCCCCCEEEEECCCHHHHHHHHHHHCCCHHHHHCCCEEECCCCCCC
AANEKFIVDEPSSNDHIAWGKVNQPFDSAKFDALFGRMLAYLHGKDIFVQECFAGCSPDH
CCCCCEEEECCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCC
RLPVRVITERAWHSLFARNMFVRATPEELVGFKPGFTVIDLPAFHAIPSVDGTNTETFII
CCCHHHHHHHHHHHHHHHCEEEEECHHHHCCCCCCCEEEECCHHHCCCCCCCCCCEEEEE
VNFEKRLIIIGGTSYAGEIKKSIFTILNYLLPQHKNVLSMHCSANVGEKDDVAVFFGLSG
EEECCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCEEEEEEECC
TGKTTLSADPRRRLIGDDEHGWDNSGVFNFEGGCYAKIINLSPEAEPEIYQTTRRFGTIL
CCCCEECCCCHHHCCCCCCCCCCCCCEEEECCCEEEEEEECCCCCCCHHHHHHHHHHHHH
ENVAIDTVSRRIDLNDDSFTENTRASYPITHIPNIVKSGMGGHPTNIIMLTCDAFGVLPP
HHHHHHHHHHEEECCCCCCCCCCCCCCCHHHCCHHHHCCCCCCCCEEEEEEECCCCCCCC
IARLTPDQAMYHFLSGYTAKVAGTEAGITEPQAAFSACFGAPFMALHPSVYAKLLGEKIA
HHHCCHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
RHGVSCWLVNTGWSGGPYGVGSRMKIAYSRALVNAAIDGTLNAGSFVKDQFFGLDIPTGC
HCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCC
SGVPAEVLNPKNTWVDKTKYDETATMLVERFRKNFEQYRSYVSAGVAEVM
CCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRLNDITRGTGLEEHGITNANLIYWTPPTAVLYEQIVKRGEGLVTHLGAVAVKTGHYTGR
CCCCCCCCCCCCHHCCCCCCEEEEECCCHHHHHHHHHHHCCCHHHHHCCCEEECCCCCCC
AANEKFIVDEPSSNDHIAWGKVNQPFDSAKFDALFGRMLAYLHGKDIFVQECFAGCSPDH
CCCCCEEEECCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCC
RLPVRVITERAWHSLFARNMFVRATPEELVGFKPGFTVIDLPAFHAIPSVDGTNTETFII
CCCHHHHHHHHHHHHHHHCEEEEECHHHHCCCCCCCEEEECCHHHCCCCCCCCCCEEEEE
VNFEKRLIIIGGTSYAGEIKKSIFTILNYLLPQHKNVLSMHCSANVGEKDDVAVFFGLSG
EEECCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCEEEEEEECC
TGKTTLSADPRRRLIGDDEHGWDNSGVFNFEGGCYAKIINLSPEAEPEIYQTTRRFGTIL
CCCCEECCCCHHHCCCCCCCCCCCCCEEEECCCEEEEEEECCCCCCCHHHHHHHHHHHHH
ENVAIDTVSRRIDLNDDSFTENTRASYPITHIPNIVKSGMGGHPTNIIMLTCDAFGVLPP
HHHHHHHHHHEEECCCCCCCCCCCCCCCHHHCCHHHHCCCCCCCCEEEEEEECCCCCCCC
IARLTPDQAMYHFLSGYTAKVAGTEAGITEPQAAFSACFGAPFMALHPSVYAKLLGEKIA
HHHCCHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
RHGVSCWLVNTGWSGGPYGVGSRMKIAYSRALVNAAIDGTLNAGSFVKDQFFGLDIPTGC
HCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCC
SGVPAEVLNPKNTWVDKTKYDETATMLVERFRKNFEQYRSYVSAGVAEVM
CCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA