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The map label for this gene is nfo [H]

Identifier: 78224187

GI number: 78224187

Start: 3378520

End: 3379377

Strand: Direct

Name: nfo [H]

Synonym: Gmet_2994

Alternate gene names: 78224187

Gene position: 3378520-3379377 (Clockwise)

Preceding gene: 78224186

Following gene: 78224188

Centisome position: 84.52

GC content: 64.22

Gene sequence:

>858_bases
ATGGATTACTTCGGCGCCCACATGTCCATCGCCGGCGGTCTCCACAACGCCCCCGACCGGGGGGTGAAGGTGGGGTGCGG
CGTCATCCAGATCTTCACCCAGAACTCCAACCAGTGGCGGGGGAAAATCCCCAGTGACTCCGACGTGGCCCTCTTCCGGG
AGAAGTGGGCGGAATCGGGGCTCCACGAGATCATCTGCCACGACATCTACCTCATCAACCTGGCGGCCCCGGAGGGGGAG
ACCCGTGACAAGAGCCTCCTCTCCTTCCGGGAGGAGATGGAGCGTTGCGCCCGCCTCGGCATCGACAAGATCGTCATGCA
CCCGGGCGCCCATGTGGGAGACGGCGAGGAGACCGGCATCAAGCGCATCTGCGAGGCCTTCGACGCGCTCATCCCCGCGG
TCCCCGAGTTCACCGGGAAGATCCTCCTGGAGACCACCGCCGGCCAAGGGACGAACCTGGGGTACACCTTCGAGCACCTG
CGGGCCATCATCGACGGCTCCGCGCACCCCGACCGCTTCGCCGTCTGCTACGACACCTGCCACACCTTCGCCGCCGGCTA
CGACATCACTACGCCGGAAGGGTACAAGCAGGTCTTCGCCGACTTCGACCGGATCATCGGCATCGACAGGCTCCAGTGCT
TCCACATCAACGACTCCAAGAAGGGGCTCAACTGCCGGGTCGACCGCCACGAGCACATCGGCCAGGGGGCCATGGGGCTC
GAAGGGTTCCGCACCCTGGTCAATGACCCGCGATTCGCCAAGGTCCCCAAAATTCTCGAAACCCCCAAGGGAGACAACGA
CGAGTTCGACGTCATGAACCTGAAGACGCTCCGGGGACTGATGGCAGGAAAGAACTGA

Upstream 100 bases:

>100_bases
GCATGTTTGTCCAAGAAACCAGTTTTTTCTCAGCTTTTCCTCCGTGCCCTCCGTCTCTCCGTGTTATTTACGACGGTTTT
ATCCGAAAGGAAGGTTACTC

Downstream 100 bases:

>100_bases
GCGATGCCCGACCTGATCGTTACCGGCTCCCTCTGCACCCGCTGCGGCGCCTGCGTTGCCACCTGTCCCGCGCGGATTAT
CGAGCTGCCGAAGGGGGTGC

Product: endonuclease IV

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV [H]

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MDYFGAHMSIAGGLHNAPDRGVKVGCGVIQIFTQNSNQWRGKIPSDSDVALFREKWAESGLHEIICHDIYLINLAAPEGE
TRDKSLLSFREEMERCARLGIDKIVMHPGAHVGDGEETGIKRICEAFDALIPAVPEFTGKILLETTAGQGTNLGYTFEHL
RAIIDGSAHPDRFAVCYDTCHTFAAGYDITTPEGYKQVFADFDRIIGIDRLQCFHINDSKKGLNCRVDRHEHIGQGAMGL
EGFRTLVNDPRFAKVPKILETPKGDNDEFDVMNLKTLRGLMAGKN

Sequences:

>Translated_285_residues
MDYFGAHMSIAGGLHNAPDRGVKVGCGVIQIFTQNSNQWRGKIPSDSDVALFREKWAESGLHEIICHDIYLINLAAPEGE
TRDKSLLSFREEMERCARLGIDKIVMHPGAHVGDGEETGIKRICEAFDALIPAVPEFTGKILLETTAGQGTNLGYTFEHL
RAIIDGSAHPDRFAVCYDTCHTFAAGYDITTPEGYKQVFADFDRIIGIDRLQCFHINDSKKGLNCRVDRHEHIGQGAMGL
EGFRTLVNDPRFAKVPKILETPKGDNDEFDVMNLKTLRGLMAGKN
>Mature_285_residues
MDYFGAHMSIAGGLHNAPDRGVKVGCGVIQIFTQNSNQWRGKIPSDSDVALFREKWAESGLHEIICHDIYLINLAAPEGE
TRDKSLLSFREEMERCARLGIDKIVMHPGAHVGDGEETGIKRICEAFDALIPAVPEFTGKILLETTAGQGTNLGYTFEHL
RAIIDGSAHPDRFAVCYDTCHTFAAGYDITTPEGYKQVFADFDRIIGIDRLQCFHINDSKKGLNCRVDRHEHIGQGAMGL
EGFRTLVNDPRFAKVPKILETPKGDNDEFDVMNLKTLRGLMAGKN

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788483, Length=269, Percent_Identity=42.3791821561338, Blast_Score=215, Evalue=3e-57,
Organism=Caenorhabditis elegans, GI17531193, Length=268, Percent_Identity=39.9253731343284, Blast_Score=194, Evalue=5e-50,
Organism=Saccharomyces cerevisiae, GI6322735, Length=268, Percent_Identity=35.0746268656716, Blast_Score=174, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307 [H]

Pfam domain/function: PF01261 AP_endonuc_2 [H]

EC number: =3.1.21.2 [H]

Molecular weight: Translated: 31593; Mature: 31593

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS00729 AP_NUCLEASE_F2_1 ; PS00730 AP_NUCLEASE_F2_2 ; PS00731 AP_NUCLEASE_F2_3 ; PS00387 PPASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYFGAHMSIAGGLHNAPDRGVKVGCGVIQIFTQNSNQWRGKIPSDSDVALFREKWAESG
CCCCCCHHHHCCCCCCCCCCCEEECCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCC
LHEIICHDIYLINLAAPEGETRDKSLLSFREEMERCARLGIDKIVMHPGAHVGDGEETGI
HHHHHHCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHEEECCCCCCCCCHHHHH
KRICEAFDALIPAVPEFTGKILLETTAGQGTNLGYTFEHLRAIIDGSAHPDRFAVCYDTC
HHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCEEHHHHHH
HTFAAGYDITTPEGYKQVFADFDRIIGIDRLQCFHINDSKKGLNCRVDRHEHIGQGAMGL
HHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCHHHCCCCCCCH
EGFRTLVNDPRFAKVPKILETPKGDNDEFDVMNLKTLRGLMAGKN
HHHHHHHCCCCHHHCCHHHHCCCCCCCCEEEHHHHHHHHHHCCCC
>Mature Secondary Structure
MDYFGAHMSIAGGLHNAPDRGVKVGCGVIQIFTQNSNQWRGKIPSDSDVALFREKWAESG
CCCCCCHHHHCCCCCCCCCCCEEECCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCC
LHEIICHDIYLINLAAPEGETRDKSLLSFREEMERCARLGIDKIVMHPGAHVGDGEETGI
HHHHHHCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHEEECCCCCCCCCHHHHH
KRICEAFDALIPAVPEFTGKILLETTAGQGTNLGYTFEHLRAIIDGSAHPDRFAVCYDTC
HHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCEEHHHHHH
HTFAAGYDITTPEGYKQVFADFDRIIGIDRLQCFHINDSKKGLNCRVDRHEHIGQGAMGL
HHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCHHHCCCCCCCH
EGFRTLVNDPRFAKVPKILETPKGDNDEFDVMNLKTLRGLMAGKN
HHHHHHHCCCCHHHCCHHHHCCCCCCCCEEEHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA