Click here to switch to the map view.

The map label for this gene is dapF

Identifier: 78224185

GI number: 78224185

Start: 3376980

End: 3377828

Strand: Direct

Name: dapF

Synonym: Gmet_2992

Alternate gene names: 78224185

Gene position: 3376980-3377828 (Clockwise)

Preceding gene: 78224179

Following gene: 78224186

Centisome position: 84.48

GC content: 64.31

Gene sequence:

>849_bases
ATGAAATTTACCAAGATGCACGGCGCCGGCAACGACTACGTCTACGTGAACTGCTTCGAGGAGCGGGTCGACAACCCGGA
GCAGGTTGCCATAAAGGTTTCCAACCGCAACTTCGGCATCGGCTCCGACGGCCTCATCCTCATCATGCCCTCCGACAAGG
CCGACGTCCGGATGCGGATGTTCAACTCCGACGGCTCCGAGTCGGAGATGTGCGGCAACGGCATCCGCTGCGTGGCCAAG
TACGCCTACGACCATGGCATCGTCTCCAAGAAGGAGATCACCGCCGAGACCGGCGCCGGCATCCTGACGCTGCAGCTGGT
GCCCGGTGCCGACGGCAAGATCGAGAAGGTGCGGGTCAACATGGGCGTTCCCCGACTGAAGCGGGCCGAGATCCCCATGG
CGGGCGAGCCCGCCGACGGGCAGATCGTCAGCCAGCCCCTCAACATCCTCCACACCACCTTCAACATCACCTGCGTCTCC
ATGGGGAACCCCCACTGCGTCATCTTCGTGGACGACGTGGAGAGCTTCCAGGTGGAAAAGTACGGCCCCCTCATCGAGAA
CCACGAGCTCTTCCCCCGCCGCACCAACGTGGAGTTCGTCCAGATCATATCCCGCACCGAGGTGCGCCAGCGGACCTGGG
AGCGGGGCGCCGGCGAAACCCTGGCCTGCGGCACCGGCGCCAGCGCCGTCTGCGTGGCCGGAGCCCTCAACTGCCTCACC
GAGAAGAAGATCCTCAACCACCTCTCGGGCGGCGACCTGGAACTGGAATGGGCCGACGACGGCTTCCTCTACATGACCGG
CCCGGCCACGGAGGTCTTCTCCGGCGAGATCGACCTGGCAAAGCTGTAA

Upstream 100 bases:

>100_bases
ACGCGGTCAAGGAGGGGAGATTTTTCCTTGTTGGCAACGGGCGTTTCTGGTATTTCTACCGGGATTCGATTCACTTTCTT
CAATAACGAGGGGAACTTCC

Downstream 100 bases:

>100_bases
TTCCGGCTGACACGACCACAGAATAAGGGCCAAAGGCCGATCGGGGTTCACTCCCCGGGCGCCTTTGGCCCTTCGTCCTT
GGAGGTATCCATGTCCACCT

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase [H]

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MKFTKMHGAGNDYVYVNCFEERVDNPEQVAIKVSNRNFGIGSDGLILIMPSDKADVRMRMFNSDGSESEMCGNGIRCVAK
YAYDHGIVSKKEITAETGAGILTLQLVPGADGKIEKVRVNMGVPRLKRAEIPMAGEPADGQIVSQPLNILHTTFNITCVS
MGNPHCVIFVDDVESFQVEKYGPLIENHELFPRRTNVEFVQIISRTEVRQRTWERGAGETLACGTGASAVCVAGALNCLT
EKKILNHLSGGDLELEWADDGFLYMTGPATEVFSGEIDLAKL

Sequences:

>Translated_282_residues
MKFTKMHGAGNDYVYVNCFEERVDNPEQVAIKVSNRNFGIGSDGLILIMPSDKADVRMRMFNSDGSESEMCGNGIRCVAK
YAYDHGIVSKKEITAETGAGILTLQLVPGADGKIEKVRVNMGVPRLKRAEIPMAGEPADGQIVSQPLNILHTTFNITCVS
MGNPHCVIFVDDVESFQVEKYGPLIENHELFPRRTNVEFVQIISRTEVRQRTWERGAGETLACGTGASAVCVAGALNCLT
EKKILNHLSGGDLELEWADDGFLYMTGPATEVFSGEIDLAKL
>Mature_282_residues
MKFTKMHGAGNDYVYVNCFEERVDNPEQVAIKVSNRNFGIGSDGLILIMPSDKADVRMRMFNSDGSESEMCGNGIRCVAK
YAYDHGIVSKKEITAETGAGILTLQLVPGADGKIEKVRVNMGVPRLKRAEIPMAGEPADGQIVSQPLNILHTTFNITCVS
MGNPHCVIFVDDVESFQVEKYGPLIENHELFPRRTNVEFVQIISRTEVRQRTWERGAGETLACGTGASAVCVAGALNCLT
EKKILNHLSGGDLELEWADDGFLYMTGPATEVFSGEIDLAKL

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family [H]

Homologues:

Organism=Escherichia coli, GI87082334, Length=282, Percent_Identity=39.0070921985816, Blast_Score=210, Evalue=7e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001653
- InterPro:   IPR018510 [H]

Pfam domain/function: PF01678 DAP_epimerase [H]

EC number: =5.1.1.7 [H]

Molecular weight: Translated: 30850; Mature: 30850

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFTKMHGAGNDYVYVNCFEERVDNPEQVAIKVSNRNFGIGSDGLILIMPSDKADVRMRM
CCEEEECCCCCCEEEEEEHHHHCCCCEEEEEEECCCCCCCCCCCEEEEEECCCCCEEEEE
FNSDGSESEMCGNGIRCVAKYAYDHGIVSKKEITAETGAGILTLQLVPGADGKIEKVRVN
ECCCCCCHHHCCCCCEEEEEHHHHCCCCCCHHCCCCCCCCEEEEEEECCCCCCEEEEEEE
MGVPRLKRAEIPMAGEPADGQIVSQPLNILHTTFNITCVSMGNPHCVIFVDDVESFQVEK
CCCCCCCCCCCCCCCCCCCCCCHHCCHHEEEEEEEEEEEEECCCEEEEEEECCCCEEEHH
YGPLIENHELFPRRTNVEFVQIISRTEVRQRTWERGAGETLACGTGASAVCVAGALNCLT
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHH
EKKILNHLSGGDLELEWADDGFLYMTGPATEVFSGEIDLAKL
HHHHHHHCCCCCEEEEECCCCEEEEECCCCEEECCCEEEECC
>Mature Secondary Structure
MKFTKMHGAGNDYVYVNCFEERVDNPEQVAIKVSNRNFGIGSDGLILIMPSDKADVRMRM
CCEEEECCCCCCEEEEEEHHHHCCCCEEEEEEECCCCCCCCCCCEEEEEECCCCCEEEEE
FNSDGSESEMCGNGIRCVAKYAYDHGIVSKKEITAETGAGILTLQLVPGADGKIEKVRVN
ECCCCCCHHHCCCCCEEEEEHHHHCCCCCCHHCCCCCCCCEEEEEEECCCCCCEEEEEEE
MGVPRLKRAEIPMAGEPADGQIVSQPLNILHTTFNITCVSMGNPHCVIFVDDVESFQVEK
CCCCCCCCCCCCCCCCCCCCCCHHCCHHEEEEEEEEEEEEECCCEEEEEEECCCCEEEHH
YGPLIENHELFPRRTNVEFVQIISRTEVRQRTWERGAGETLACGTGASAVCVAGALNCLT
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHH
EKKILNHLSGGDLELEWADDGFLYMTGPATEVFSGEIDLAKL
HHHHHHHCCCCCEEEEECCCCEEEEECCCCEEECCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA