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The map label for this gene is fixL [H]

Identifier: 78224151

GI number: 78224151

Start: 3344799

End: 3347141

Strand: Reverse

Name: fixL [H]

Synonym: Gmet_2956

Alternate gene names: 78224151

Gene position: 3347141-3344799 (Counterclockwise)

Preceding gene: 78224153

Following gene: 78224150

Centisome position: 83.73

GC content: 61.89

Gene sequence:

>2343_bases
ATGACTATAAAGACGAAAACCAGTGAGGAGTTGCTGGCGGAAAACAAGGACCTCCGCGCTCGCCTTGTTGAGGCGGAAAA
GCGTGCCCGCGAGGCCGAATCGCAGGCCCGGATGGCGGAAGAGCTGACCCGGCGATTGGCCGAGGAGGAGGTGGAGCGGA
AAAGCGCCGTGCTGGCGCGGAAGGAAAGCGAAACGCGGTTCCTGGCGGCCCAGGAGCTTTCACTGGACGGCTTTACGTTG
ATGGAAGCTGTCCGGGACGATGGTGGACATGTGGTGGATTTCCGCTGGACATACGTGAACCCGCCGGCAAGCCGGATGTT
GCGGCAACCACAGGCAGAGCTGGTGGGCCGGCGGCTGCTGGAGGTCCTGCCCGGAAACCAGGAGAACAGCGACCTCTTCG
AGCGCTACGTTCAGGTTGTCGAGACGGGCGAACCCCATGATTACGAGCTTCACTACCAGTCCGAGGGGGTTGACGGCTGG
TTCCGCAACATGACCATCAAGCTCGGCGACGGCATTGCCGTCTGTTTCTCCGACATTACCCAGCGCAGAAAAACCGAAAA
GGAACTGCGCGCGAGCGAGGAACGTTCGGAACTGTTGTCGCGGACCATCAGCGCCCTGCTGACCGCCGCGGATCCCCAGG
ACGTGGTCGAGCAATTATGCAATGAGGTTCGGGAATTCCTGCAGTGCGCGGTTTTTTTCAACTATCTCTTCGATCCCCAC
ACCGGGAGGCTCAAATTCAACGCCTGCGGCGGGGTAGACAGGCGGCTGGCGCGGCTTGTGGAAGATCTGGAGCTTTCCGG
ATCCCTCTGCGGCACGGCTGCCCAGGGCCGCTGCCGGGTTCATGCGGAGAACCTCGGGTCCAGCGCCGATCCGCGCAGTT
CCCTGGTCCGGAGCCTCGGCATTCGCGCCTATGCCTGCCATCCGCTGCTGGGCCCGGCCAACGAAGCCATCGGGACCCTC
TCCTTCGGGGCGACCGACAGGGATTCGTTCAGCAGTGACGATCTGGAGCTGATGAAGACGGTCGCCGACCATGTGGCCGT
CGCCATGCTTCGGCGCAAGGCGGAGAAGGAATTGCAGGCGGACTTGGCCGCCCTTACGCGCATGCACGAGTTAAGTGGCA
AGCTGCTGGAAACGGCGGGTCTCCAGCAGCTCCTCCAGGAGGCGATGGACGCGGCATTAAGCATCGTCGGCGCGGCCAAG
GGGACGCTGCAATTGCTCGAAGCCGATACGTTGCACATTGTTGCGCACCACGGCCACGAGCCGCCGTTCCTGGATTTTTT
TACGGCCGCCGAAAACGTCGCCTCCACGTGTGGCAAGGCGATGCAGTGCGGCGAACGGGTGGTGGTCCACGACGTGGAGG
AAAGCCCGTTGTTCGCCGGCACGCCGTCGTTGTCGGTGCTGCGCGCCGCCGGGGTGCGCGCGATGCAATCCACGCCGCTC
TTCACCCGCTCCGGCCGGGTGCTCGGCGTCCTTACCACGCATTGGGACTCGCCGCACGTGCCCGATGAGCACGACCTGTG
GCGGATGGACCTGCTGGCCAGACAGGTCACCGACCTCATCGAACGGACGCAGGCCGAAGAGGAGCTTAGAAAGCTGAACG
AGATTCTGGAACAGCGGGTCGCCGAACGGACGGCGCAGTTGCGGGAAAAGGACCGGATGCTGCTGGTTCAGGGGCGCCAG
GCAGCCATGGGTGAGATGATCGGCAATATCGCCCACCAGTGGCGGCAACCCCTGAACACCCTGGGGCTCACCATCCAGCA
GTTGCCCCTGTTCTACGATCTTGGCGAGTTCAACAGGGAATTCCTGGAGCAGGCGGTCAACCGGTCGATGGAGCTTATCC
AGTACATGTCCAAGACCATCGATGATTTCAGAAACTATTTCAAGCCGGACAAGGCCCGGATCGATTTCAGCGGGCGCGAG
ACAATCGCCCGGTCCCTGTCGCTCATCGGGGACAATCTACGGCACCAGCAGATCGATGTTGAGCTGAAGGTGCTGGATGA
TGTAGTTATAACCGGCTATGAGAACGAATTCGCCCAGGCGCTGCTCAACATCCTGGTCAATGCCAGGGATGCCCTGACGG
AGAGGGGCGTGCAGCACCCGAAGATCACCATCGCCATCGGCGCCGAAGGCCGGAAGGCGGTCGTGACCATTGCCGACAAT
GCCGGCGGCATCCCGGAAGAGATCATAGACAAGATTTTTAATCCCTACTTCACCACCAAGGGACCCCATCAGGGGTCAGG
GGTGGGGTTGTTCATGGCGAAGACGATCATCGAGAACAACATGGGGGGGAAGCTGACGGTGTGCAACACGGCCGACGGCG
CAGAGTTCAGGATCGAGGTGTGA

Upstream 100 bases:

>100_bases
CGCGCCCTTGTTCGTCAAGCTGCACCATCCGCCGTTGCGAGCCAGTATCCGGCTCAGGAAGAACCGGTTCTCCTCGAAAC
GGGCGCAGAGGCTTCAGTCC

Downstream 100 bases:

>100_bases
CGGTGACTGCTGAAACGCCGGGGAGGCCGGTTTCAATGCTGCTGGTGGAGGATGAAGAAGAGACGCGGACTTGACCGCTG
CACCCGCCCGGACCTACCGT

Product: multi-sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 780; Mature: 779

Protein sequence:

>780_residues
MTIKTKTSEELLAENKDLRARLVEAEKRAREAESQARMAEELTRRLAEEEVERKSAVLARKESETRFLAAQELSLDGFTL
MEAVRDDGGHVVDFRWTYVNPPASRMLRQPQAELVGRRLLEVLPGNQENSDLFERYVQVVETGEPHDYELHYQSEGVDGW
FRNMTIKLGDGIAVCFSDITQRRKTEKELRASEERSELLSRTISALLTAADPQDVVEQLCNEVREFLQCAVFFNYLFDPH
TGRLKFNACGGVDRRLARLVEDLELSGSLCGTAAQGRCRVHAENLGSSADPRSSLVRSLGIRAYACHPLLGPANEAIGTL
SFGATDRDSFSSDDLELMKTVADHVAVAMLRRKAEKELQADLAALTRMHELSGKLLETAGLQQLLQEAMDAALSIVGAAK
GTLQLLEADTLHIVAHHGHEPPFLDFFTAAENVASTCGKAMQCGERVVVHDVEESPLFAGTPSLSVLRAAGVRAMQSTPL
FTRSGRVLGVLTTHWDSPHVPDEHDLWRMDLLARQVTDLIERTQAEEELRKLNEILEQRVAERTAQLREKDRMLLVQGRQ
AAMGEMIGNIAHQWRQPLNTLGLTIQQLPLFYDLGEFNREFLEQAVNRSMELIQYMSKTIDDFRNYFKPDKARIDFSGRE
TIARSLSLIGDNLRHQQIDVELKVLDDVVITGYENEFAQALLNILVNARDALTERGVQHPKITIAIGAEGRKAVVTIADN
AGGIPEEIIDKIFNPYFTTKGPHQGSGVGLFMAKTIIENNMGGKLTVCNTADGAEFRIEV

Sequences:

>Translated_780_residues
MTIKTKTSEELLAENKDLRARLVEAEKRAREAESQARMAEELTRRLAEEEVERKSAVLARKESETRFLAAQELSLDGFTL
MEAVRDDGGHVVDFRWTYVNPPASRMLRQPQAELVGRRLLEVLPGNQENSDLFERYVQVVETGEPHDYELHYQSEGVDGW
FRNMTIKLGDGIAVCFSDITQRRKTEKELRASEERSELLSRTISALLTAADPQDVVEQLCNEVREFLQCAVFFNYLFDPH
TGRLKFNACGGVDRRLARLVEDLELSGSLCGTAAQGRCRVHAENLGSSADPRSSLVRSLGIRAYACHPLLGPANEAIGTL
SFGATDRDSFSSDDLELMKTVADHVAVAMLRRKAEKELQADLAALTRMHELSGKLLETAGLQQLLQEAMDAALSIVGAAK
GTLQLLEADTLHIVAHHGHEPPFLDFFTAAENVASTCGKAMQCGERVVVHDVEESPLFAGTPSLSVLRAAGVRAMQSTPL
FTRSGRVLGVLTTHWDSPHVPDEHDLWRMDLLARQVTDLIERTQAEEELRKLNEILEQRVAERTAQLREKDRMLLVQGRQ
AAMGEMIGNIAHQWRQPLNTLGLTIQQLPLFYDLGEFNREFLEQAVNRSMELIQYMSKTIDDFRNYFKPDKARIDFSGRE
TIARSLSLIGDNLRHQQIDVELKVLDDVVITGYENEFAQALLNILVNARDALTERGVQHPKITIAIGAEGRKAVVTIADN
AGGIPEEIIDKIFNPYFTTKGPHQGSGVGLFMAKTIIENNMGGKLTVCNTADGAEFRIEV
>Mature_779_residues
TIKTKTSEELLAENKDLRARLVEAEKRAREAESQARMAEELTRRLAEEEVERKSAVLARKESETRFLAAQELSLDGFTLM
EAVRDDGGHVVDFRWTYVNPPASRMLRQPQAELVGRRLLEVLPGNQENSDLFERYVQVVETGEPHDYELHYQSEGVDGWF
RNMTIKLGDGIAVCFSDITQRRKTEKELRASEERSELLSRTISALLTAADPQDVVEQLCNEVREFLQCAVFFNYLFDPHT
GRLKFNACGGVDRRLARLVEDLELSGSLCGTAAQGRCRVHAENLGSSADPRSSLVRSLGIRAYACHPLLGPANEAIGTLS
FGATDRDSFSSDDLELMKTVADHVAVAMLRRKAEKELQADLAALTRMHELSGKLLETAGLQQLLQEAMDAALSIVGAAKG
TLQLLEADTLHIVAHHGHEPPFLDFFTAAENVASTCGKAMQCGERVVVHDVEESPLFAGTPSLSVLRAAGVRAMQSTPLF
TRSGRVLGVLTTHWDSPHVPDEHDLWRMDLLARQVTDLIERTQAEEELRKLNEILEQRVAERTAQLREKDRMLLVQGRQA
AMGEMIGNIAHQWRQPLNTLGLTIQQLPLFYDLGEFNREFLEQAVNRSMELIQYMSKTIDDFRNYFKPDKARIDFSGRET
IARSLSLIGDNLRHQQIDVELKVLDDVVITGYENEFAQALLNILVNARDALTERGVQHPKITIAIGAEGRKAVVTIADNA
GGIPEEIIDKIFNPYFTTKGPHQGSGVGLFMAKTIIENNMGGKLTVCNTADGAEFRIEV

Specific function: Putative oxygen sensor; modulates the activity of fixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates fixJ [H]

COG id: COG2203

COG function: function code T; FOG: GAF domain

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=229, Percent_Identity=25.3275109170306, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 87038; Mature: 86907

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIKTKTSEELLAENKDLRARLVEAEKRAREAESQARMAEELTRRLAEEEVERKSAVLAR
CCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KESETRFLAAQELSLDGFTLMEAVRDDGGHVVDFRWTYVNPPASRMLRQPQAELVGRRLL
CCCCHHHHHHHHCCCCHHHHHHHHHCCCCEEEEEEEEEECCHHHHHHCCCHHHHHHHHHH
EVLPGNQENSDLFERYVQVVETGEPHDYELHYQSEGVDGWFRNMTIKLGDGIAVCFSDIT
HHCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHCEEEEECCCHHHHHHHHH
QRRKTEKELRASEERSELLSRTISALLTAADPQDVVEQLCNEVREFLQCAVFFNYLFDPH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TGRLKFNACGGVDRRLARLVEDLELSGSLCGTAAQGRCRVHAENLGSSADPRSSLVRSLG
CCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEHHHCCCCCCHHHHHHHHHC
IRAYACHPLLGPANEAIGTLSFGATDRDSFSSDDLELMKTVADHVAVAMLRRKAEKELQA
CCEEEECCCCCCCCHHCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLAALTRMHELSGKLLETAGLQQLLQEAMDAALSIVGAAKGTLQLLEADTLHIVAHHGHE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCEEEEEECCCC
PPFLDFFTAAENVASTCGKAMQCGERVVVHDVEESPLFAGTPSLSVLRAAGVRAMQSTPL
CCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCC
FTRSGRVLGVLTTHWDSPHVPDEHDLWRMDLLARQVTDLIERTQAEEELRKLNEILEQRV
EECCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AERTAQLREKDRMLLVQGRQAAMGEMIGNIAHQWRQPLNTLGLTIQQLPLFYDLGEFNRE
HHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHH
FLEQAVNRSMELIQYMSKTIDDFRNYFKPDKARIDFSGRETIARSLSLIGDNLRHQQIDV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCHHHHHHHHHHHHHCCCCHHHHCE
ELKVLDDVVITGYENEFAQALLNILVNARDALTERGVQHPKITIAIGAEGRKAVVTIADN
EEEEECCHHEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEEECC
AGGIPEEIIDKIFNPYFTTKGPHQGSGVGLFMAKTIIENNMGGKLTVCNTADGAEFRIEV
CCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEC
>Mature Secondary Structure 
TIKTKTSEELLAENKDLRARLVEAEKRAREAESQARMAEELTRRLAEEEVERKSAVLAR
CCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KESETRFLAAQELSLDGFTLMEAVRDDGGHVVDFRWTYVNPPASRMLRQPQAELVGRRLL
CCCCHHHHHHHHCCCCHHHHHHHHHCCCCEEEEEEEEEECCHHHHHHCCCHHHHHHHHHH
EVLPGNQENSDLFERYVQVVETGEPHDYELHYQSEGVDGWFRNMTIKLGDGIAVCFSDIT
HHCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHCEEEEECCCHHHHHHHHH
QRRKTEKELRASEERSELLSRTISALLTAADPQDVVEQLCNEVREFLQCAVFFNYLFDPH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TGRLKFNACGGVDRRLARLVEDLELSGSLCGTAAQGRCRVHAENLGSSADPRSSLVRSLG
CCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEHHHCCCCCCHHHHHHHHHC
IRAYACHPLLGPANEAIGTLSFGATDRDSFSSDDLELMKTVADHVAVAMLRRKAEKELQA
CCEEEECCCCCCCCHHCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLAALTRMHELSGKLLETAGLQQLLQEAMDAALSIVGAAKGTLQLLEADTLHIVAHHGHE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCEEEEEECCCC
PPFLDFFTAAENVASTCGKAMQCGERVVVHDVEESPLFAGTPSLSVLRAAGVRAMQSTPL
CCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCC
FTRSGRVLGVLTTHWDSPHVPDEHDLWRMDLLARQVTDLIERTQAEEELRKLNEILEQRV
EECCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AERTAQLREKDRMLLVQGRQAAMGEMIGNIAHQWRQPLNTLGLTIQQLPLFYDLGEFNRE
HHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHH
FLEQAVNRSMELIQYMSKTIDDFRNYFKPDKARIDFSGRETIARSLSLIGDNLRHQQIDV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCHHHHHHHHHHHHHCCCCHHHHCE
ELKVLDDVVITGYENEFAQALLNILVNARDALTERGVQHPKITIAIGAEGRKAVVTIADN
EEEEECCHHEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEEECC
AGGIPEEIIDKIFNPYFTTKGPHQGSGVGLFMAKTIIENNMGGKLTVCNTADGAEFRIEV
CCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2842062; 11481432; 8432704 [H]