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The map label for this gene is rnhB

Identifier: 78224061

GI number: 78224061

Start: 3249333

End: 3249974

Strand: Reverse

Name: rnhB

Synonym: Gmet_2865

Alternate gene names: 78224061

Gene position: 3249974-3249333 (Counterclockwise)

Preceding gene: 78224062

Following gene: 78224060

Centisome position: 81.3

GC content: 56.39

Gene sequence:

>642_bases
GTGCGTCTACCGTTGTTTGACGACATTGGTGAGAGATCCCTCTGGACATTTGAAAACCTTGTCAGCCGAAATGGTTTCCA
CGCGGTAGCGGGGGTTGACGAGGCCGGACGCGGTCCCTTGGCCGGTCCTGTCGTGGCGGCTGCAGTCATTCTTCCGCAAG
GGGCAGAGTTGCCGGGGGTCGACGACTCAAAGAAGCTTTCTGCACCAAAGCGCGAACATCTCTTCGGCCTGATATCCGGT
TGTGCCCTGGCCGTAGGTGTCGGAGTGGCTGACCACGCCTGCATTGATCGCATCAACATACTTCAGGCCACTCTCCGCGC
CATGGCGGAAGCTGTCGGACAACTGGCCGTTCCTCCTGATTACCTCCTCATAGATGGAATTTCAACGATTCCCCTTAACC
TCCCTCAGAAAACCATCAAAAAAGGGGACAGCTCCAGCATTTCCATCGCATCCGCCTCAATCGTTGCCAAGGTTACCCGG
GACCGGATGATGATGGAATATGATCAACAGTTCCCCGGTTACGGATTCGCCGAGCACAAGGGCTATGGCTGTGCCTCTCA
CCTTGCTGCGATTGCGGAACTGGGTCCATGCCCCATCCACCGGAAAACCTTCAGGGGGGTGAAAGAGCATGTCGCTCCAT
AA

Upstream 100 bases:

>100_bases
CTCCGCGGCAAGGCCGCGCGGATTCGCGAGAAGAAGTACGTGGCTGCCCACTAACGAAATTGCCCCGCCTGGCGGGGCTT
TTTCATTTCAGGAGAGATCG

Downstream 100 bases:

>100_bases
CAAGTCCCTGGGCAGCAAGGGGGAATCCCTGGCGGTGACATATCTGGAACGTCATCGTTACAAAATCCTGGAGAGAAACT
TTCGTTGCCGCGGCGGCGAG

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MRLPLFDDIGERSLWTFENLVSRNGFHAVAGVDEAGRGPLAGPVVAAAVILPQGAELPGVDDSKKLSAPKREHLFGLISG
CALAVGVGVADHACIDRINILQATLRAMAEAVGQLAVPPDYLLIDGISTIPLNLPQKTIKKGDSSSISIASASIVAKVTR
DRMMMEYDQQFPGYGFAEHKGYGCASHLAAIAELGPCPIHRKTFRGVKEHVAP

Sequences:

>Translated_213_residues
MRLPLFDDIGERSLWTFENLVSRNGFHAVAGVDEAGRGPLAGPVVAAAVILPQGAELPGVDDSKKLSAPKREHLFGLISG
CALAVGVGVADHACIDRINILQATLRAMAEAVGQLAVPPDYLLIDGISTIPLNLPQKTIKKGDSSSISIASASIVAKVTR
DRMMMEYDQQFPGYGFAEHKGYGCASHLAAIAELGPCPIHRKTFRGVKEHVAP
>Mature_213_residues
MRLPLFDDIGERSLWTFENLVSRNGFHAVAGVDEAGRGPLAGPVVAAAVILPQGAELPGVDDSKKLSAPKREHLFGLISG
CALAVGVGVADHACIDRINILQATLRAMAEAVGQLAVPPDYLLIDGISTIPLNLPQKTIKKGDSSSISIASASIVAKVTR
DRMMMEYDQQFPGYGFAEHKGYGCASHLAAIAELGPCPIHRKTFRGVKEHVAP

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=180, Percent_Identity=50, Blast_Score=156, Evalue=1e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_GEOMG (Q39RP1)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_385808.1
- ProteinModelPortal:   Q39RP1
- SMR:   Q39RP1
- STRING:   Q39RP1
- GeneID:   3738922
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_2865
- NMPDR:   fig|269799.3.peg.3035
- eggNOG:   COG0164
- HOGENOM:   HBG584843
- OMA:   RLGPTPI
- PhylomeDB:   Q39RP1
- ProtClustDB:   PRK00015
- BioCyc:   GMET269799:GMET_2865-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 22575; Mature: 22575

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLPLFDDIGERSLWTFENLVSRNGFHAVAGVDEAGRGPLAGPVVAAAVILPQGAELPGV
CCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCCC
DDSKKLSAPKREHLFGLISGCALAVGVGVADHACIDRINILQATLRAMAEAVGQLAVPPD
CCCHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
YLLIDGISTIPLNLPQKTIKKGDSSSISIASASIVAKVTRDRMMMEYDQQFPGYGFAEHK
EEEECCCCCCCCCCCHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCC
GYGCASHLAAIAELGPCPIHRKTFRGVKEHVAP
CCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MRLPLFDDIGERSLWTFENLVSRNGFHAVAGVDEAGRGPLAGPVVAAAVILPQGAELPGV
CCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCCC
DDSKKLSAPKREHLFGLISGCALAVGVGVADHACIDRINILQATLRAMAEAVGQLAVPPD
CCCHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
YLLIDGISTIPLNLPQKTIKKGDSSSISIASASIVAKVTRDRMMMEYDQQFPGYGFAEHK
EEEECCCCCCCCCCCHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCC
GYGCASHLAAIAELGPCPIHRKTFRGVKEHVAP
CCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA