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The map label for this gene is sigB [H]

Identifier: 78224050

GI number: 78224050

Start: 3233085

End: 3233924

Strand: Direct

Name: sigB [H]

Synonym: Gmet_2854

Alternate gene names: 78224050

Gene position: 3233085-3233924 (Clockwise)

Preceding gene: 78224035

Following gene: 78224051

Centisome position: 80.88

GC content: 58.45

Gene sequence:

>840_bases
ATGACCATGAATCTCCCCGTTGTTGCCGACAGCTTCACCCTCTACCTGGCGGAAATCAGGAAGTTCCCCGTCCTCACCCC
CGAGGAGGAACACCTGTACGCCGTAAAGTTCTATGAAGAGAAGGACCTGGAGGCGGCCCACCGCCTCATCACCGCCAACC
TCCGATTTGTGGTGAAGGTCGCTGCCGAGTACAAATCCTACGGCATGAAGATGCTCGACCTGATCCAGGAGGGGAACATC
GGCCTCATGATGGCGGTCCGCAAATTCGACCCCCACAAGGGGATCCGTCTCATCTCCTACGCGGTCTGGTGGATCAGGGC
CTACATCCAGAATTACATCATCTCTGCCTGGAGCCTTCTCAAGATCGGCACCACCCAGGCCCAGAAGAAACTCTTCTTCA
AGCTGAACCAGGCCAAGAATGCCATCCGGCGGCTGACCGGGGGTGACGACACGGAAGCCACCGCCCTCTCCCTCCACGTG
AAAGAGTCGGAAGTGATCGAAATGGACCAGCGGATGCGGGGCGACTACTCTCTGGACGCTGAACTGGTGGACGGGGAAGG
GCTCACCATGCTGGATACCCTTGTGGACGAGCGCCAGAACCAGGAGGAAGCCTTGGCAGAGCAGCAGGAGGCGTCACGCC
TCCACGGCCAGGTGACCCAGGCACTGGAACGGCTCAACGAAAAGGAGCGCTACGTAGTCGAGAATCGGGTAACGGCAGAC
GAACCACTTACCCTCCAGGAGATCGCCGACCATTTCGCAATCTCCCGCGAGCGGGTCCGTCAGATTGAGGAAGGGGCACT
GAAAAAGATAAAGGCCTATCTGACGCCAATAACTGTCTAG

Upstream 100 bases:

>100_bases
AACCCCTTGATTTTTCTTTTTTCCCTTGTTAAGTTATTAGCACTCAGACAAACCGAGTGCTAACCCAGTAACAAACCTTT
GCGACAGGAGGACCCGATTC

Downstream 100 bases:

>100_bases
CCCCCCAAACCATTTTCTCATTGATACATCCAGACCCCGCCTTGACGAGACGGGGTTTTTTTATTATTACTGTCAAGAAA
TACTCATCACCAACGGGGAG

Product: sigma 32 (RpoH)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MTMNLPVVADSFTLYLAEIRKFPVLTPEEEHLYAVKFYEEKDLEAAHRLITANLRFVVKVAAEYKSYGMKMLDLIQEGNI
GLMMAVRKFDPHKGIRLISYAVWWIRAYIQNYIISAWSLLKIGTTQAQKKLFFKLNQAKNAIRRLTGGDDTEATALSLHV
KESEVIEMDQRMRGDYSLDAELVDGEGLTMLDTLVDERQNQEEALAEQQEASRLHGQVTQALERLNEKERYVVENRVTAD
EPLTLQEIADHFAISRERVRQIEEGALKKIKAYLTPITV

Sequences:

>Translated_279_residues
MTMNLPVVADSFTLYLAEIRKFPVLTPEEEHLYAVKFYEEKDLEAAHRLITANLRFVVKVAAEYKSYGMKMLDLIQEGNI
GLMMAVRKFDPHKGIRLISYAVWWIRAYIQNYIISAWSLLKIGTTQAQKKLFFKLNQAKNAIRRLTGGDDTEATALSLHV
KESEVIEMDQRMRGDYSLDAELVDGEGLTMLDTLVDERQNQEEALAEQQEASRLHGQVTQALERLNEKERYVVENRVTAD
EPLTLQEIADHFAISRERVRQIEEGALKKIKAYLTPITV
>Mature_278_residues
TMNLPVVADSFTLYLAEIRKFPVLTPEEEHLYAVKFYEEKDLEAAHRLITANLRFVVKVAAEYKSYGMKMLDLIQEGNIG
LMMAVRKFDPHKGIRLISYAVWWIRAYIQNYIISAWSLLKIGTTQAQKKLFFKLNQAKNAIRRLTGGDDTEATALSLHVK
ESEVIEMDQRMRGDYSLDAELVDGEGLTMLDTLVDERQNQEEALAEQQEASRLHGQVTQALERLNEKERYVVENRVTADE
PLTLQEIADHFAISRERVRQIEEGALKKIKAYLTPITV

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=263, Percent_Identity=38.4030418250951, Blast_Score=177, Evalue=8e-46,
Organism=Escherichia coli, GI1789098, Length=269, Percent_Identity=33.457249070632, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1789448, Length=236, Percent_Identity=30.5084745762712, Blast_Score=97, Evalue=2e-21,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 32064; Mature: 31933

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMNLPVVADSFTLYLAEIRKFPVLTPEEEHLYAVKFYEEKDLEAAHRLITANLRFVVKV
CCCCCCEEHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHH
AAEYKSYGMKMLDLIQEGNIGLMMAVRKFDPHKGIRLISYAVWWIRAYIQNYIISAWSLL
HHHHHHHCHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KIGTTQAQKKLFFKLNQAKNAIRRLTGGDDTEATALSLHVKESEVIEMDQRMRGDYSLDA
HCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECHHHHHHHHHHHCCCCCCCC
ELVDGEGLTMLDTLVDERQNQEEALAEQQEASRLHGQVTQALERLNEKERYVVENRVTAD
EEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCC
EPLTLQEIADHFAISRERVRQIEEGALKKIKAYLTPITV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TMNLPVVADSFTLYLAEIRKFPVLTPEEEHLYAVKFYEEKDLEAAHRLITANLRFVVKV
CCCCCEEHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHH
AAEYKSYGMKMLDLIQEGNIGLMMAVRKFDPHKGIRLISYAVWWIRAYIQNYIISAWSLL
HHHHHHHCHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KIGTTQAQKKLFFKLNQAKNAIRRLTGGDDTEATALSLHVKESEVIEMDQRMRGDYSLDA
HCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECHHHHHHHHHHHCCCCCCCC
ELVDGEGLTMLDTLVDERQNQEEALAEQQEASRLHGQVTQALERLNEKERYVVENRVTAD
EEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCC
EPLTLQEIADHFAISRERVRQIEEGALKKIKAYLTPITV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1475207 [H]