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The map label for this gene is phoB [H]

Identifier: 78224023

GI number: 78224023

Start: 3203512

End: 3203922

Strand: Reverse

Name: phoB [H]

Synonym: Gmet_2827

Alternate gene names: 78224023

Gene position: 3203922-3203512 (Counterclockwise)

Preceding gene: 78224024

Following gene: 78224022

Centisome position: 80.15

GC content: 58.39

Gene sequence:

>411_bases
GTGGCAACAGACCCTCAGGATACGGCACAGGTCGTGATCGTGGACGACGAACCTTTCATCCGGGATGCGGTGGTTAAACT
CCTCGCGTGTGAGGGAATCGCCGCGGTAGCCGCGGAGAACGGGGACGAATGCCTGGACCTTTTGCGGAAAGGGTTCAGGG
GAATCATTCTCATGGACGTCATGATGCCCCGCAGAAACGGCTGGCAAACCATCCGCGCCATGGCGGACGAATCCCTTGTG
GCCGGCAATATCATCTCCATGCTGACCTCGGTCGACGAGCCCGATGAACAGATGGAAGGGTTGCAGGAACTGGTGTTCGA
CTACATCACGAAGCCTTTCGACCCTTCCGAGATGGTTGCAACGGTACGCACGTACCTTGGTTGCCTGGAGCAGATGCGAG
GCGGGAACTGA

Upstream 100 bases:

>100_bases
CGTTTCGCCGGTCACGGGAACTGGCCGCTGACCAGGCTTTCCGAAAGCGGCGAAGAGGGTTCACGCAGGTACCGGTTCAC
GCTTCAGGCAGGAGGCATAT

Downstream 100 bases:

>100_bases
GCCATGCCATTGCCCAACCTGGCGGTCATAGGGGCATCGACGGGAGGACTCAAGGTGTTTGAGTCGCTGTTTCCCCATCT
TCCGGTCCTCAATGCCGGTG

Product: response regulator receiver domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 136; Mature: 135

Protein sequence:

>136_residues
MATDPQDTAQVVIVDDEPFIRDAVVKLLACEGIAAVAAENGDECLDLLRKGFRGIILMDVMMPRRNGWQTIRAMADESLV
AGNIISMLTSVDEPDEQMEGLQELVFDYITKPFDPSEMVATVRTYLGCLEQMRGGN

Sequences:

>Translated_136_residues
MATDPQDTAQVVIVDDEPFIRDAVVKLLACEGIAAVAAENGDECLDLLRKGFRGIILMDVMMPRRNGWQTIRAMADESLV
AGNIISMLTSVDEPDEQMEGLQELVFDYITKPFDPSEMVATVRTYLGCLEQMRGGN
>Mature_135_residues
ATDPQDTAQVVIVDDEPFIRDAVVKLLACEGIAAVAAENGDECLDLLRKGFRGIILMDVMMPRRNGWQTIRAMADESLVA
GNIISMLTSVDEPDEQMEGLQELVFDYITKPFDPSEMVATVRTYLGCLEQMRGGN

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=123, Percent_Identity=33.3333333333333, Blast_Score=74, Evalue=3e-15,
Organism=Escherichia coli, GI1790863, Length=120, Percent_Identity=30, Blast_Score=61, Evalue=3e-11,
Organism=Escherichia coli, GI1786784, Length=117, Percent_Identity=32.4786324786325, Blast_Score=61, Evalue=3e-11,
Organism=Escherichia coli, GI1788394, Length=121, Percent_Identity=31.404958677686, Blast_Score=60, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 15013; Mature: 14882

Theoretical pI: Translated: 3.94; Mature: 3.94

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
6.6 %Met     (Translated Protein)
8.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
5.9 %Met     (Mature Protein)
8.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATDPQDTAQVVIVDDEPFIRDAVVKLLACEGIAAVAAENGDECLDLLRKGFRGIILMDV
CCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHCHHHHHHH
MMPRRNGWQTIRAMADESLVAGNIISMLTSVDEPDEQMEGLQELVFDYITKPFDPSEMVA
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHH
TVRTYLGCLEQMRGGN
HHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ATDPQDTAQVVIVDDEPFIRDAVVKLLACEGIAAVAAENGDECLDLLRKGFRGIILMDV
CCCCCCCCEEEEECCCCHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHCHHHHHHH
MMPRRNGWQTIRAMADESLVAGNIISMLTSVDEPDEQMEGLQELVFDYITKPFDPSEMVA
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHH
TVRTYLGCLEQMRGGN
HHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]