Click here to switch to the map view.

The map label for this gene is dfrA [H]

Identifier: 78224017

GI number: 78224017

Start: 3193957

End: 3194943

Strand: Direct

Name: dfrA [H]

Synonym: Gmet_2821

Alternate gene names: 78224017

Gene position: 3193957-3194943 (Clockwise)

Preceding gene: 78224016

Following gene: 78224018

Centisome position: 79.9

GC content: 64.44

Gene sequence:

>987_bases
ATGAAGGTTTTCGTTACCGGCGCAACCGGCTTCATAGGCGCTAGCATAGCAAGGGAACTGCTGAAGGACGGCTGCCATGT
CAGGGTTCTGGCCCGGCCCGGCTCCGACAGGCGCAATCTCGATGGCCTCGATGTGGAAGTGTGCGAGGGGGACCTCTGTT
CCCCGGAATCCCTGGACCGGGGGGTCAAGGGGTGCGAGGTCCTCTACCACGCCGCGGCCGACTACCGCCTCTGGACCCGC
AACCCCGCCGCCATGTACGCCGCCAACGTGGAGGGAACCCGCCACGTCCTGGACGCGGCCCTGCGGCACGGGGTATCCCG
GGTCGTCTACACGAGCAGCGTCGGGACCCTGGGAAATCCCGGCGACGGCACCCCCGGCACGGAAGCCACCCCCGTCACCT
TCGCCGACATGGTGGGGGACTACAAGAAAAGCAAATTTCTGGCGGAACGGGAGGCTGAGACGTTCCTGGCCCGGGGACTC
CCCCTGGTCATCGTGAATCCCTCGACCCCGGTGGGCCCCCACGACGTGAAACCGACTCCCACCGGAAAGATCATCGTCGA
TTTCCTCAACCGCGCCATGCCCGCCTACCTGGACACGGGGCTCAACATCATCGACGTTGAGGACTGCGCCCAGGGACACA
TCCTGGCGGCTCAGCACGGCAGAATCGGCGAGAAGTACATCCTCGGCCACGAGAATCTGACGTTGCGGCAGATATTCTCT
CTCCTCGAAACGGTTACGGGACTCGCAGCGCCGAAGGTGCGCCTCCCCTACTTACCTATCCTCGCAGCCGCCTACGCAAA
CGAGGCCCTGTCGCGGATCACCGGCAGGGAGCCCCTCATCCCGCTGGCAGGGGTGCAGATGGCCAGAAAATTCATGTATT
TCGATTCGTCCAAGGCGGTGAAGGAACTGGGGCTTCCCCAGCGCCCGGCCGTCGAAGCCCTGGGGCGTGCGGTGGAATGG
TTCCGCGCCAACGGATATGTACGGTAA

Upstream 100 bases:

>100_bases
GCTGAAGGGCCACAAGGGATAACCAGCCAGTCGCAGAAACGGGCACCTGCGTAGGGGCGGTTCAAGAACCGCCCCTACAT
CAGTTTTGATAGAAGGAACC

Downstream 100 bases:

>100_bases
ATATGGAGGGGCGCAATTCATTGCTCCCCGGGCGTGGTGAATCACGCCCCTACGCAACCGATGAAGGAGAGCGTCATGTA
CAAGCTGCTCGACACCATAA

Product: NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: DFR; Dihydrokaempferol 4-reductase [H]

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MKVFVTGATGFIGASIARELLKDGCHVRVLARPGSDRRNLDGLDVEVCEGDLCSPESLDRGVKGCEVLYHAAADYRLWTR
NPAAMYAANVEGTRHVLDAALRHGVSRVVYTSSVGTLGNPGDGTPGTEATPVTFADMVGDYKKSKFLAEREAETFLARGL
PLVIVNPSTPVGPHDVKPTPTGKIIVDFLNRAMPAYLDTGLNIIDVEDCAQGHILAAQHGRIGEKYILGHENLTLRQIFS
LLETVTGLAAPKVRLPYLPILAAAYANEALSRITGREPLIPLAGVQMARKFMYFDSSKAVKELGLPQRPAVEALGRAVEW
FRANGYVR

Sequences:

>Translated_328_residues
MKVFVTGATGFIGASIARELLKDGCHVRVLARPGSDRRNLDGLDVEVCEGDLCSPESLDRGVKGCEVLYHAAADYRLWTR
NPAAMYAANVEGTRHVLDAALRHGVSRVVYTSSVGTLGNPGDGTPGTEATPVTFADMVGDYKKSKFLAEREAETFLARGL
PLVIVNPSTPVGPHDVKPTPTGKIIVDFLNRAMPAYLDTGLNIIDVEDCAQGHILAAQHGRIGEKYILGHENLTLRQIFS
LLETVTGLAAPKVRLPYLPILAAAYANEALSRITGREPLIPLAGVQMARKFMYFDSSKAVKELGLPQRPAVEALGRAVEW
FRANGYVR
>Mature_328_residues
MKVFVTGATGFIGASIARELLKDGCHVRVLARPGSDRRNLDGLDVEVCEGDLCSPESLDRGVKGCEVLYHAAADYRLWTR
NPAAMYAANVEGTRHVLDAALRHGVSRVVYTSSVGTLGNPGDGTPGTEATPVTFADMVGDYKKSKFLAEREAETFLARGL
PLVIVNPSTPVGPHDVKPTPTGKIIVDFLNRAMPAYLDTGLNIIDVEDCAQGHILAAQHGRIGEKYILGHENLTLRQIFS
LLETVTGLAAPKVRLPYLPILAAAYANEALSRITGREPLIPLAGVQMARKFMYFDSSKAVKELGLPQRPAVEALGRAVEW
FRANGYVR

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dihydroflavonol-4-reductase family [H]

Homologues:

Organism=Homo sapiens, GI193211614, Length=339, Percent_Identity=26.8436578171091, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI8393516, Length=339, Percent_Identity=26.8436578171091, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI116268111, Length=355, Percent_Identity=25.3521126760563, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI239745448, Length=335, Percent_Identity=25.9701492537313, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI310132178, Length=335, Percent_Identity=25.6716417910448, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI310113012, Length=335, Percent_Identity=25.6716417910448, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71987463, Length=338, Percent_Identity=29.2899408284024, Blast_Score=96, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6321437, Length=349, Percent_Identity=26.3610315186246, Blast_Score=79, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR017829
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.219 [H]

Molecular weight: Translated: 35477; Mature: 35477

Theoretical pI: Translated: 7.92; Mature: 7.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVFVTGATGFIGASIARELLKDGCHVRVLARPGSDRRNLDGLDVEVCEGDLCSPESLDR
CEEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCEEEECCCCCCCHHHHHH
GVKGCEVLYHAAADYRLWTRNPAAMYAANVEGTRHVLDAALRHGVSRVVYTSSVGTLGNP
HHHHHHHHHHHHCCCEEEECCCHHEEEECCCHHHHHHHHHHHHCHHHEEEECCCCCCCCC
GDGTPGTEATPVTFADMVGDYKKSKFLAEREAETFLARGLPLVIVNPSTPVGPHDVKPTP
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCC
TGKIIVDFLNRAMPAYLDTGLNIIDVEDCAQGHILAAQHGRIGEKYILGHENLTLRQIFS
CHHHHHHHHHHHHHHHHHCCCCEEEHHHCCCCCEEEECCCCCCCEEEECCCCCCHHHHHH
LLETVTGLAAPKVRLPYLPILAAAYANEALSRITGREPLIPLAGVQMARKFMYFDSSKAV
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHH
KELGLPQRPAVEALGRAVEWFRANGYVR
HHCCCCCCHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKVFVTGATGFIGASIARELLKDGCHVRVLARPGSDRRNLDGLDVEVCEGDLCSPESLDR
CEEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCEEEECCCCCCCHHHHHH
GVKGCEVLYHAAADYRLWTRNPAAMYAANVEGTRHVLDAALRHGVSRVVYTSSVGTLGNP
HHHHHHHHHHHHCCCEEEECCCHHEEEECCCHHHHHHHHHHHHCHHHEEEECCCCCCCCC
GDGTPGTEATPVTFADMVGDYKKSKFLAEREAETFLARGLPLVIVNPSTPVGPHDVKPTP
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCC
TGKIIVDFLNRAMPAYLDTGLNIIDVEDCAQGHILAAQHGRIGEKYILGHENLTLRQIFS
CHHHHHHHHHHHHHHHHHCCCCEEEHHHCCCCCEEEECCCCCCCEEEECCCCCCHHHHHH
LLETVTGLAAPKVRLPYLPILAAAYANEALSRITGREPLIPLAGVQMARKFMYFDSSKAV
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHH
KELGLPQRPAVEALGRAVEWFRANGYVR
HHCCCCCCHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]