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The map label for this gene is leuC1 [H]

Identifier: 78223959

GI number: 78223959

Start: 3121329

End: 3123275

Strand: Reverse

Name: leuC1 [H]

Synonym: Gmet_2763

Alternate gene names: 78223959

Gene position: 3123275-3121329 (Counterclockwise)

Preceding gene: 78223960

Following gene: 78223958

Centisome position: 78.13

GC content: 66.2

Gene sequence:

>1947_bases
ATGGGAAAAAATCTCGCAACCAAGATCCTCGAAGCGCACCTCGCGAAGGGCGAGCTTAAGCCGGGCACCGAGATCGCCCT
GAAGATCGACCACGCGCTCTTGCAGGACGCCACCGGCACCATGGCCATGCTGGAGTTCATCGCCATGGGGGTCGACCGGG
TGAAGGTGGAGCTGGCGGCCCAGTACATCGACCATAACCTCCTCCAGACCGACAACCGCAACGCCGACGACCACATCTTT
CTCATGACCGCGGCCCAGCGGTTCGGCATCCACGTCTCCAAGCCGGGGAACGGGGTCTCCCACCAGGTGCACCTGGAGCG
CTTCGGCGTGCCGGGCAAGACCATGCTCGGGGCCGACTCCCACACCACCTCCGCCGCCGGTCTTTCCATGATCGCCATCG
GCGCCGGGGGGCTCGATGTCTCACTGGCCATGGCGGGGCACCCCTTCCATCTCCCCTGTCCGAAGATCTGGGGAGTGAAG
CTCACGGGAGAGCTCCAGCCCTGGGTCTCGGCCAAGGATGTGATCCTGGAGATGCTCCGCCGCCACACGGTGAAGGGGGG
GGTCGGGAAGATCATCGAGTACTACGGCCCCGGCGTGGCGACCCTCTCGGCCACGGACCGGGCCATCATCGGCAACATGG
GGGCGGAACTGGGGGCCACCACCTCCGTCTTCCCCTCGGACCACCGGACCCGCGAGTTCCTGGAGGCCCAGGGGCGCGGC
CAGCTCTGGCAGGAGCTTGCCGCCGACCCCGACGCCACCTACGACGAGTACGAGGAGATTGACCTCGCCACGGTGGAACC
CCTCATCGCCCGGCCGTCATCCCCCGACAACGTGGTGCGGGTGGCCGACATCGAGGGGTTGAAGGTGGACCAGGTGCTGG
TGGGGAGTTCCGCCAACTCCGCCTTCCGGGACCTCATGACCGTCTGTCGCATCCTGGATGGCCGGCGCATCGCCCCCCAC
CTCTCCTTCAACGTGAACCCGGGGAGCCGCCAGGTGCTGGAAAACGTGGCGGATCAAGGCGGGATCATGATGTTGCTTCT
GGCCGGCGCCCAGATCCACCAGCCGGGGTGCCTCGGCTGCATCGGCATGGGGCAAGCGCCGGGGACCAATCAGGTGAGCC
TCCGCACCTTCCCCCGCAACTTCCCGGGGAGAAGCGGCACGAAGAACGATCTGGTCTACCTCTGCTCCCCCGAGACGGCA
GCCGCCGCCGGCCTCTTCGGCGTGGTCACCGACCCCCGCAAGCTGGAGGGCCTCATGCCGTGGCCCAACGTCCAGAACCC
TGAGAACTACATCCTGGACGACTCCAGCATCATCTTCCCCCTCCCCCCCGAGGAGGCGAAGCGGGTGGAGATCGTCACCG
GCCCCAACATCATCCCGTTCCCCGACTACGATCCCCTCCCCGAGACCTTGGAGGCGGAGGTCATCCTCACCGTGGGGGAC
AATATCTCCACCGACACCATCATGCCTGCGGGGAATAAGGTGCTCCCCTACCGGAGCAACATCCCGGCCATCAGCCAGTT
CGTCTTCGAGCATCTGGACCCCGATTTCCACAAGCGGGCCAAGGAGAAGGGGAACGGCGTCGTCATCGGCGGGGAGAACT
ACGGCCAGGGGTCGAGCCGGGAGCATGCGGCCCTGGCGCCGCGCTATCTCGGCATCCGGGCCAAGATCGTCAAGAGCTTC
GCCCGGATCCACAAGGCGAACCTGGTGAACTTCGGCATCCTCCCCCTCACGTTCAGAAATCCCGCCGACTACGACCTGGT
GAAGCAGGGGGACCGCCTCGTGTTCCCCGATGTGCGCCGGCTCGTGGCCTCCGGCGCCGCAGAGATACCGGTGCGGGTGG
ACGGACGGGAGATTGTCACCATTCTGGAGGTCTCAGACCGGCAGCGGCAGGAGCTTCTGGCCGGGGGGACCCTGAACTTT
GTGAAAAAAGGCGGTACGGAGGGGTGA

Upstream 100 bases:

>100_bases
CCATGACCTTCCACGCCCACCCGACCCTGGCCGAGGCCATGAAGGAGGCGGCCCTGGATGTGGAGAAACGGGCGATACAT
GCGTGAACACGGAGGTTCCC

Downstream 100 bases:

>100_bases
GGGCATAAGGGAATGGATGGGCCGTGGAGCACAAGCCGGTGAGTCTGAACAGAGCGACCCTATGGGCACTGCTGGTATTT
TCGCTTTTCATGGGGATGGG

Product: aconitate hydratase

Products: NA

Alternate protein names: Alpha-IPM isomerase 1; IPMI 1; Isopropylmalate isomerase 1 [H]

Number of amino acids: Translated: 648; Mature: 647

Protein sequence:

>648_residues
MGKNLATKILEAHLAKGELKPGTEIALKIDHALLQDATGTMAMLEFIAMGVDRVKVELAAQYIDHNLLQTDNRNADDHIF
LMTAAQRFGIHVSKPGNGVSHQVHLERFGVPGKTMLGADSHTTSAAGLSMIAIGAGGLDVSLAMAGHPFHLPCPKIWGVK
LTGELQPWVSAKDVILEMLRRHTVKGGVGKIIEYYGPGVATLSATDRAIIGNMGAELGATTSVFPSDHRTREFLEAQGRG
QLWQELAADPDATYDEYEEIDLATVEPLIARPSSPDNVVRVADIEGLKVDQVLVGSSANSAFRDLMTVCRILDGRRIAPH
LSFNVNPGSRQVLENVADQGGIMMLLLAGAQIHQPGCLGCIGMGQAPGTNQVSLRTFPRNFPGRSGTKNDLVYLCSPETA
AAAGLFGVVTDPRKLEGLMPWPNVQNPENYILDDSSIIFPLPPEEAKRVEIVTGPNIIPFPDYDPLPETLEAEVILTVGD
NISTDTIMPAGNKVLPYRSNIPAISQFVFEHLDPDFHKRAKEKGNGVVIGGENYGQGSSREHAALAPRYLGIRAKIVKSF
ARIHKANLVNFGILPLTFRNPADYDLVKQGDRLVFPDVRRLVASGAAEIPVRVDGREIVTILEVSDRQRQELLAGGTLNF
VKKGGTEG

Sequences:

>Translated_648_residues
MGKNLATKILEAHLAKGELKPGTEIALKIDHALLQDATGTMAMLEFIAMGVDRVKVELAAQYIDHNLLQTDNRNADDHIF
LMTAAQRFGIHVSKPGNGVSHQVHLERFGVPGKTMLGADSHTTSAAGLSMIAIGAGGLDVSLAMAGHPFHLPCPKIWGVK
LTGELQPWVSAKDVILEMLRRHTVKGGVGKIIEYYGPGVATLSATDRAIIGNMGAELGATTSVFPSDHRTREFLEAQGRG
QLWQELAADPDATYDEYEEIDLATVEPLIARPSSPDNVVRVADIEGLKVDQVLVGSSANSAFRDLMTVCRILDGRRIAPH
LSFNVNPGSRQVLENVADQGGIMMLLLAGAQIHQPGCLGCIGMGQAPGTNQVSLRTFPRNFPGRSGTKNDLVYLCSPETA
AAAGLFGVVTDPRKLEGLMPWPNVQNPENYILDDSSIIFPLPPEEAKRVEIVTGPNIIPFPDYDPLPETLEAEVILTVGD
NISTDTIMPAGNKVLPYRSNIPAISQFVFEHLDPDFHKRAKEKGNGVVIGGENYGQGSSREHAALAPRYLGIRAKIVKSF
ARIHKANLVNFGILPLTFRNPADYDLVKQGDRLVFPDVRRLVASGAAEIPVRVDGREIVTILEVSDRQRQELLAGGTLNF
VKKGGTEG
>Mature_647_residues
GKNLATKILEAHLAKGELKPGTEIALKIDHALLQDATGTMAMLEFIAMGVDRVKVELAAQYIDHNLLQTDNRNADDHIFL
MTAAQRFGIHVSKPGNGVSHQVHLERFGVPGKTMLGADSHTTSAAGLSMIAIGAGGLDVSLAMAGHPFHLPCPKIWGVKL
TGELQPWVSAKDVILEMLRRHTVKGGVGKIIEYYGPGVATLSATDRAIIGNMGAELGATTSVFPSDHRTREFLEAQGRGQ
LWQELAADPDATYDEYEEIDLATVEPLIARPSSPDNVVRVADIEGLKVDQVLVGSSANSAFRDLMTVCRILDGRRIAPHL
SFNVNPGSRQVLENVADQGGIMMLLLAGAQIHQPGCLGCIGMGQAPGTNQVSLRTFPRNFPGRSGTKNDLVYLCSPETAA
AAGLFGVVTDPRKLEGLMPWPNVQNPENYILDDSSIIFPLPPEEAKRVEIVTGPNIIPFPDYDPLPETLEAEVILTVGDN
ISTDTIMPAGNKVLPYRSNIPAISQFVFEHLDPDFHKRAKEKGNGVVIGGENYGQGSSREHAALAPRYLGIRAKIVKSFA
RIHKANLVNFGILPLTFRNPADYDLVKQGDRLVFPDVRRLVASGAAEIPVRVDGREIVTILEVSDRQRQELLAGGTLNFV
KKGGTEG

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG1048

COG function: function code C; Aconitase A

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=718, Percent_Identity=33.5654596100279, Blast_Score=317, Evalue=2e-86,
Organism=Homo sapiens, GI8659555, Length=822, Percent_Identity=25.3041362530414, Blast_Score=188, Evalue=2e-47,
Organism=Homo sapiens, GI41352693, Length=740, Percent_Identity=24.0540540540541, Blast_Score=153, Evalue=4e-37,
Organism=Escherichia coli, GI87081781, Length=728, Percent_Identity=26.3736263736264, Blast_Score=208, Evalue=7e-55,
Organism=Escherichia coli, GI1787531, Length=746, Percent_Identity=26.5415549597855, Blast_Score=171, Evalue=2e-43,
Organism=Escherichia coli, GI1786259, Length=471, Percent_Identity=26.7515923566879, Blast_Score=132, Evalue=7e-32,
Organism=Escherichia coli, GI2367097, Length=429, Percent_Identity=23.3100233100233, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI25149337, Length=717, Percent_Identity=33.4728033472803, Blast_Score=304, Evalue=1e-82,
Organism=Caenorhabditis elegans, GI32564738, Length=688, Percent_Identity=33.8662790697674, Blast_Score=299, Evalue=2e-81,
Organism=Caenorhabditis elegans, GI25149342, Length=568, Percent_Identity=33.4507042253521, Blast_Score=241, Evalue=1e-63,
Organism=Caenorhabditis elegans, GI17568399, Length=754, Percent_Identity=25.0663129973475, Blast_Score=167, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6322261, Length=731, Percent_Identity=33.6525307797538, Blast_Score=333, Evalue=5e-92,
Organism=Saccharomyces cerevisiae, GI6323335, Length=715, Percent_Identity=34.2657342657343, Blast_Score=331, Evalue=3e-91,
Organism=Saccharomyces cerevisiae, GI6320440, Length=617, Percent_Identity=27.2285251215559, Blast_Score=172, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6321429, Length=750, Percent_Identity=24, Blast_Score=140, Evalue=5e-34,
Organism=Drosophila melanogaster, GI28571643, Length=722, Percent_Identity=31.994459833795, Blast_Score=314, Evalue=1e-85,
Organism=Drosophila melanogaster, GI281365315, Length=719, Percent_Identity=32.6842837273992, Blast_Score=308, Evalue=6e-84,
Organism=Drosophila melanogaster, GI17864292, Length=719, Percent_Identity=32.6842837273992, Blast_Score=308, Evalue=6e-84,
Organism=Drosophila melanogaster, GI161076999, Length=686, Percent_Identity=32.798833819242, Blast_Score=302, Evalue=6e-82,
Organism=Drosophila melanogaster, GI24645686, Length=761, Percent_Identity=26.8068331143233, Blast_Score=167, Evalue=2e-41,
Organism=Drosophila melanogaster, GI17137564, Length=777, Percent_Identity=25.3539253539254, Blast_Score=164, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 69975; Mature: 69844

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKNLATKILEAHLAKGELKPGTEIALKIDHALLQDATGTMAMLEFIAMGVDRVKVELAA
CCCHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHCCCCHHHHHHHHHHCHHHHHHHHHH
QYIDHNLLQTDNRNADDHIFLMTAAQRFGIHVSKPGNGVSHQVHLERFGVPGKTMLGADS
HHHHHHHHCCCCCCCCCEEEEEEEHHHHCEEEECCCCCCCEEEEEECCCCCCCEEECCCC
HTTSAAGLSMIAIGAGGLDVSLAMAGHPFHLPCPKIWGVKLTGELQPWVSAKDVILEMLR
CCCCCCCEEEEEEECCCCEEEEEECCCCCCCCCCCEECEEEECCCCCCCCHHHHHHHHHH
RHTVKGGVGKIIEYYGPGVATLSATDRAIIGNMGAELGATTSVFPSDHRTREFLEAQGRG
HHHCCCCHHHHHHHHCCCEEEECCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCH
QLWQELAADPDATYDEYEEIDLATVEPLIARPSSPDNVVRVADIEGLKVDQVLVGSSANS
HHHHHHCCCCCCCCHHHHHCCHHHHCHHHCCCCCCCCEEEEECCCCCEEEEEEECCCCCH
AFRDLMTVCRILDGRRIAPHLSFNVNPGSRQVLENVADQGGIMMLLLAGAQIHQPGCLGC
HHHHHHHHHHHHCCCEECCEEEEECCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEE
IGMGQAPGTNQVSLRTFPRNFPGRSGTKNDLVYLCSPETAAAAGLFGVVTDPRKLEGLMP
EECCCCCCCCEEEEEECCCCCCCCCCCCCCEEEEECCCHHHHHCEEEEECCHHHHCCCCC
WPNVQNPENYILDDSSIIFPLPPEEAKRVEIVTGPNIIPFPDYDPLPETLEAEVILTVGD
CCCCCCCCCEEECCCCEEEECCCCCCCEEEEEECCCEECCCCCCCCCCCCCCEEEEEECC
NISTDTIMPAGNKVLPYRSNIPAISQFVFEHLDPDFHKRAKEKGNGVVIGGENYGQGSSR
CCCCCEECCCCCEECCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCEEEECCCCCCCCCCC
EHAALAPRYLGIRAKIVKSFARIHKANLVNFGILPLTFRNPADYDLVKQGDRLVFPDVRR
CCHHHCCHHHHHHHHHHHHHHHHHHHCEEEECEEEEEECCCCCCHHHHCCCEEECHHHHH
LVASGAAEIPVRVDGREIVTILEVSDRQRQELLAGGTLNFVKKGGTEG
HHHCCCCCCEEEECCCEEEEEEECCCHHHHHHHCCCCCHHHHCCCCCC
>Mature Secondary Structure 
GKNLATKILEAHLAKGELKPGTEIALKIDHALLQDATGTMAMLEFIAMGVDRVKVELAA
CCHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHCCCCHHHHHHHHHHCHHHHHHHHHH
QYIDHNLLQTDNRNADDHIFLMTAAQRFGIHVSKPGNGVSHQVHLERFGVPGKTMLGADS
HHHHHHHHCCCCCCCCCEEEEEEEHHHHCEEEECCCCCCCEEEEEECCCCCCCEEECCCC
HTTSAAGLSMIAIGAGGLDVSLAMAGHPFHLPCPKIWGVKLTGELQPWVSAKDVILEMLR
CCCCCCCEEEEEEECCCCEEEEEECCCCCCCCCCCEECEEEECCCCCCCCHHHHHHHHHH
RHTVKGGVGKIIEYYGPGVATLSATDRAIIGNMGAELGATTSVFPSDHRTREFLEAQGRG
HHHCCCCHHHHHHHHCCCEEEECCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCH
QLWQELAADPDATYDEYEEIDLATVEPLIARPSSPDNVVRVADIEGLKVDQVLVGSSANS
HHHHHHCCCCCCCCHHHHHCCHHHHCHHHCCCCCCCCEEEEECCCCCEEEEEEECCCCCH
AFRDLMTVCRILDGRRIAPHLSFNVNPGSRQVLENVADQGGIMMLLLAGAQIHQPGCLGC
HHHHHHHHHHHHCCCEECCEEEEECCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEE
IGMGQAPGTNQVSLRTFPRNFPGRSGTKNDLVYLCSPETAAAAGLFGVVTDPRKLEGLMP
EECCCCCCCCEEEEEECCCCCCCCCCCCCCEEEEECCCHHHHHCEEEEECCHHHHCCCCC
WPNVQNPENYILDDSSIIFPLPPEEAKRVEIVTGPNIIPFPDYDPLPETLEAEVILTVGD
CCCCCCCCCEEECCCCEEEECCCCCCCEEEEEECCCEECCCCCCCCCCCCCCEEEEEECC
NISTDTIMPAGNKVLPYRSNIPAISQFVFEHLDPDFHKRAKEKGNGVVIGGENYGQGSSR
CCCCCEECCCCCEECCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCEEEECCCCCCCCCCC
EHAALAPRYLGIRAKIVKSFARIHKANLVNFGILPLTFRNPADYDLVKQGDRLVFPDVRR
CCHHHCCHHHHHHHHHHHHHHHHHHHCEEEECEEEEEECCCCCCHHHHCCCEEECHHHHH
LVASGAAEIPVRVDGREIVTILEVSDRQRQELLAGGTLNFVKKGGTEG
HHHCCCCCCEEEECCCEEEEEEECCCHHHHHHHCCCCCHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]