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The map label for this gene is cheB4

Identifier: 78223907

GI number: 78223907

Start: 3061837

End: 3062880

Strand: Direct

Name: cheB4

Synonym: Gmet_2711

Alternate gene names: 78223907

Gene position: 3061837-3062880 (Clockwise)

Preceding gene: 78223906

Following gene: 78223908

Centisome position: 76.6

GC content: 65.71

Gene sequence:

>1044_bases
ATGGTGAAGGTTCTCATTGTGGACGACTCGGCGAGCGTGCGGCTGTTCCTTCAGGGGCTGCTCGCCGCCGACCCGGGGAT
AGAGGTGATCGGCACCGCGGCGGACGGGGATGAAGCTGTCGAAGCGGTGGCGCGGCTGAATCCCGACGTGGTAACCATGG
ACATCTACATGCCGCGGATGAACGGCCTGCTCGCCACCCGCCGGATCATGGAAACCCACCCGGTCCCCATCGTCGTGGTG
AGCGGAAACCTTGACGCCGAGGAAGTCGCCTCGACCTTCCGTGCCATAGAGGCGGGGGCCGTCACCGCCCTGCCGCGCCC
CCAGGGCCCCGGCCATCCGAACCACGAGCGGGAAGCCAGGTCCTTCGTCCAGGCCGTGAAGCTGATGGCTGAAGTCAAGG
TGATAAAGCGGTGGCCCCGACGCGACAACCCGGCCTCCCTCCCTTCTTTGCCATGCCAGGCGATGGTTCCGGCGCGGCTC
AAGGCGGTAGCCATCGGCGCTTCGACCGGCGGGCCCATGGTTCTCCAGACCATTCTTGCCGGTCTGCGAAGGGATTTCCC
GCTGCCGGTCCTCATCGTCCAGCACATGGCCACGGGCTTCATCAAGGGATTTGTCGAGTGGCTCAACCTCACCTCGGCCC
TGCCGGTGCACATTGCCGGCAACGGCGACCGACTCATCCCCGGCCACGCCTACGTGGCGCCGGACTGTTTTCACATGCTC
GTGACCGAAGACGGAACTGCAATCGAGTTGCAGAACACCCCCCCGGAAAACGGCCTCCGACCATCGGTCTCTGCCCTCTT
CCGCTCCGTGACGCAGGCTTTTGGCGCCCAGACGGTCGGTGTGCTCCTCACCGGCATGGGAAGCGACGGCGCCAGGGAAC
TGAAGCGCTTACGCGAGGCCGGAGCCGTCACCATCGTGCAGGACCGGGAAAGCTCCGTCATTCACGGAATGCCGGGCGAG
GCCCTGAAACTGGGGGCAGCGACCCACACGCTCCCTCCCGACCGGATCGTGACCGCCCTTACCAGCCTGGCTATGGAGAA
GTGA

Upstream 100 bases:

>100_bases
TGCCGGGGCAAACGCCTATATCGTGAAGAGCAGTTTCGACCAGAGCAATCTGCTGGATGCCATACGGAGGCTGATTTGAG
CTGTGGAGTAAGAGGCTGAG

Downstream 100 bases:

>100_bases
CATGGGAGCAACCACGGAACAAAGCAACGGCAACATCCTCATCATCGAAGACAGCCCCACCCAGTCAGCACAACTTCGCC
ACATCATGGAAACGAACGGC

Product: response regulator receiver (CheY-like) modulated CheB methylesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MVKVLIVDDSASVRLFLQGLLAADPGIEVIGTAADGDEAVEAVARLNPDVVTMDIYMPRMNGLLATRRIMETHPVPIVVV
SGNLDAEEVASTFRAIEAGAVTALPRPQGPGHPNHEREARSFVQAVKLMAEVKVIKRWPRRDNPASLPSLPCQAMVPARL
KAVAIGASTGGPMVLQTILAGLRRDFPLPVLIVQHMATGFIKGFVEWLNLTSALPVHIAGNGDRLIPGHAYVAPDCFHML
VTEDGTAIELQNTPPENGLRPSVSALFRSVTQAFGAQTVGVLLTGMGSDGARELKRLREAGAVTIVQDRESSVIHGMPGE
ALKLGAATHTLPPDRIVTALTSLAMEK

Sequences:

>Translated_347_residues
MVKVLIVDDSASVRLFLQGLLAADPGIEVIGTAADGDEAVEAVARLNPDVVTMDIYMPRMNGLLATRRIMETHPVPIVVV
SGNLDAEEVASTFRAIEAGAVTALPRPQGPGHPNHEREARSFVQAVKLMAEVKVIKRWPRRDNPASLPSLPCQAMVPARL
KAVAIGASTGGPMVLQTILAGLRRDFPLPVLIVQHMATGFIKGFVEWLNLTSALPVHIAGNGDRLIPGHAYVAPDCFHML
VTEDGTAIELQNTPPENGLRPSVSALFRSVTQAFGAQTVGVLLTGMGSDGARELKRLREAGAVTIVQDRESSVIHGMPGE
ALKLGAATHTLPPDRIVTALTSLAMEK
>Mature_347_residues
MVKVLIVDDSASVRLFLQGLLAADPGIEVIGTAADGDEAVEAVARLNPDVVTMDIYMPRMNGLLATRRIMETHPVPIVVV
SGNLDAEEVASTFRAIEAGAVTALPRPQGPGHPNHEREARSFVQAVKLMAEVKVIKRWPRRDNPASLPSLPCQAMVPARL
KAVAIGASTGGPMVLQTILAGLRRDFPLPVLIVQHMATGFIKGFVEWLNLTSALPVHIAGNGDRLIPGHAYVAPDCFHML
VTEDGTAIELQNTPPENGLRPSVSALFRSVTQAFGAQTVGVLLTGMGSDGARELKRLREAGAVTIVQDRESSVIHGMPGE
ALKLGAATHTLPPDRIVTALTSLAMEK

Specific function: Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR

COG id: COG2201

COG function: function code NT; Chemotaxis response regulator containing a CheY-like receiver domain and a methylesterase domain

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain

Homologues:

Organism=Escherichia coli, GI1788192, Length=326, Percent_Identity=36.5030674846626, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI1790299, Length=105, Percent_Identity=35.2380952380952, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CHEB4_GEOMG (Q39S45)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_385654.1
- ProteinModelPortal:   Q39S45
- STRING:   Q39S45
- GeneID:   3740018
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_2711
- NMPDR:   fig|269799.3.peg.3225
- eggNOG:   COG2201
- HOGENOM:   HBG705324
- OMA:   GPGHPNH
- PhylomeDB:   Q39S45
- ProtClustDB:   CLSK755255
- BioCyc:   GMET269799:GMET_2711-MONOMER
- GO:   GO:0005737
- GO:   GO:0006935
- HAMAP:   MF_00099
- InterPro:   IPR011006
- InterPro:   IPR008248
- InterPro:   IPR000673
- InterPro:   IPR001789
- Gene3D:   G3DSA:3.40.50.180
- PIRSF:   PIRSF000876
- SMART:   SM00448

Pfam domain/function: PF01339 CheB_methylest; PF00072 Response_reg; SSF52738 Chemotax_RR_pGlu_Me-esterase; SSF52172 CheY_like

EC number: =3.1.1.61

Molecular weight: Translated: 36897; Mature: 36897

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS50122 CHEB; PS50110 RESPONSE_REGULATORY

Important sites: ACT_SITE 168-168 ACT_SITE 195-195 ACT_SITE 289-289

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKVLIVDDSASVRLFLQGLLAADPGIEVIGTAADGDEAVEAVARLNPDVVTMDIYMPRM
CEEEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCEEEEEEECCCH
NGLLATRRIMETHPVPIVVVSGNLDAEEVASTFRAIEAGAVTALPRPQGPGHPNHEREAR
HHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCCHHHHHH
SFVQAVKLMAEVKVIKRWPRRDNPASLPSLPCQAMVPARLKAVAIGASTGGPMVLQTILA
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCEEEEEEECCCCCHHHHHHHHH
GLRRDFPLPVLIVQHMATGFIKGFVEWLNLTSALPVHIAGNGDRLIPGHAYVAPDCFHML
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEECCCCCEECCHHEEEE
VTEDGTAIELQNTPPENGLRPSVSALFRSVTQAFGAQTVGVLLTGMGSDGARELKRLREA
EECCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCEEEHEEECCCCCHHHHHHHHHHC
GAVTIVQDRESSVIHGMPGEALKLGAATHTLPPDRIVTALTSLAMEK
CCEEEEECCCCCEECCCCCCCEEECCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVKVLIVDDSASVRLFLQGLLAADPGIEVIGTAADGDEAVEAVARLNPDVVTMDIYMPRM
CEEEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCEEEEEEECCCH
NGLLATRRIMETHPVPIVVVSGNLDAEEVASTFRAIEAGAVTALPRPQGPGHPNHEREAR
HHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCCHHHHHH
SFVQAVKLMAEVKVIKRWPRRDNPASLPSLPCQAMVPARLKAVAIGASTGGPMVLQTILA
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCEEEEEEECCCCCHHHHHHHHH
GLRRDFPLPVLIVQHMATGFIKGFVEWLNLTSALPVHIAGNGDRLIPGHAYVAPDCFHML
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEECCCCCEECCHHEEEE
VTEDGTAIELQNTPPENGLRPSVSALFRSVTQAFGAQTVGVLLTGMGSDGARELKRLREA
EECCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCEEEHEEECCCCCHHHHHHHHHHC
GAVTIVQDRESSVIHGMPGEALKLGAATHTLPPDRIVTALTSLAMEK
CCEEEEECCCCCEECCCCCCCEEECCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA