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The map label for this gene is pstS [H]

Identifier: 78223897

GI number: 78223897

Start: 3050053

End: 3051081

Strand: Direct

Name: pstS [H]

Synonym: Gmet_2701

Alternate gene names: 78223897

Gene position: 3050053-3051081 (Clockwise)

Preceding gene: 78223896

Following gene: 78223898

Centisome position: 76.3

GC content: 61.22

Gene sequence:

>1029_bases
ATGCTCAAAGCATTACGGAAAAGTCTCGCGATCCTCGCGACTCTGGCAGTAACCGCCGCGGCCGGCCAGGCCGGTGCGGA
AACCCTCATCAACGGCGCAGGCGCCACCTTCCCCTACCCGCTCTACTCCAAGTGGTTCAGCGAGTACGCCAAGATCGATC
CCTCGGTGAAATTCAACTACCAGTCCATCGGCAGCGGCGGCGGCATCAAGCAGATCACTGCCCAGACCGTCGACTTCGGC
GCCAGCGACAAGTTCCTGAGCGATGCGGAACTGAAGGCGGCCCCCGGCAAGCTCCTCCACATTCCGACCGTCATGGGTGC
GGTCGTCGTAACCTACAACCTTCCGGGCGCTCCGAAGGGGCTGAAGCTCAAGGCTGAAGACGTGGCCGACATCTTCCTCG
GCAAGATCAGCAAGTGGAACGATCCGCGCATCGCAGACGACAACCCCGGCGTCAAGCTTCCCAATAGCCCGATCATCGTC
GTGCACCGCTCCGACGGCAGCGGCACCACCAGCATCTTCACCGACTTCCTCTCCGGCGTGAACCAGGAGTGGGCCAAGAA
GGTCGGCAAAGGTGCCTCGGTCAAGTGGCCCATCGGCCTCGGCGGCAAGGGGAACGAGGGTGTTGCCGGCCAGATCAAGA
CCACTGCAAACTCCATCGGCTATGTTGAGCTTGCCTATGCCTTCGAGAACAAGCTCCCCTACGCGACCCTCAAGAACAAG
AGCGGCAGCTGGGTAGTCCCCTCCATCCAGACGACGAGCGCAGCGGCAGCCGCAGCCGTCAAACACATGCCGGCTGATTA
CCGGATCTCCCTGGTGAACCAGCCGGGCAAGGACGCCTACCCCATCGTCGGCTTCACCTGGCTCCTCGTGTACGAAAAGC
AGAAAGACGCCGTCAAAGGGAAAAAGCTGGTGGAATTCCTGAACTGGAGCCTCCGCAACGGCCAGAAGATGGCAGCACCG
CTCCTCTACGCGCCGCTCCCCGAGAACGTCAAGAAGATGGTGGAGAAGACCATCAAGACCGTCAAGTAA

Upstream 100 bases:

>100_bases
CAGGTTTTTCAGAGGATCTTTGCATGGTCGTAACCTGAATGTAACAGAAGCCAGCCTATTATCACGCATGTGTTGAAAAC
AAATTCCCAGGAGGAACCAG

Downstream 100 bases:

>100_bases
TCAGCGCCTGAAGCCCGGCGGCCCGCTTCAGTGAAGCGGCGTCCGCCGGGCATCTTTGTGTGTCTGCGATTGTTACAGCA
TCATTAAAATCATGCAACAA

Product: periplasmic phosphate binding protein

Products: ADP; phosphate [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: PBP [H]

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MLKALRKSLAILATLAVTAAAGQAGAETLINGAGATFPYPLYSKWFSEYAKIDPSVKFNYQSIGSGGGIKQITAQTVDFG
ASDKFLSDAELKAAPGKLLHIPTVMGAVVVTYNLPGAPKGLKLKAEDVADIFLGKISKWNDPRIADDNPGVKLPNSPIIV
VHRSDGSGTTSIFTDFLSGVNQEWAKKVGKGASVKWPIGLGGKGNEGVAGQIKTTANSIGYVELAYAFENKLPYATLKNK
SGSWVVPSIQTTSAAAAAAVKHMPADYRISLVNQPGKDAYPIVGFTWLLVYEKQKDAVKGKKLVEFLNWSLRNGQKMAAP
LLYAPLPENVKKMVEKTIKTVK

Sequences:

>Translated_342_residues
MLKALRKSLAILATLAVTAAAGQAGAETLINGAGATFPYPLYSKWFSEYAKIDPSVKFNYQSIGSGGGIKQITAQTVDFG
ASDKFLSDAELKAAPGKLLHIPTVMGAVVVTYNLPGAPKGLKLKAEDVADIFLGKISKWNDPRIADDNPGVKLPNSPIIV
VHRSDGSGTTSIFTDFLSGVNQEWAKKVGKGASVKWPIGLGGKGNEGVAGQIKTTANSIGYVELAYAFENKLPYATLKNK
SGSWVVPSIQTTSAAAAAAVKHMPADYRISLVNQPGKDAYPIVGFTWLLVYEKQKDAVKGKKLVEFLNWSLRNGQKMAAP
LLYAPLPENVKKMVEKTIKTVK
>Mature_342_residues
MLKALRKSLAILATLAVTAAAGQAGAETLINGAGATFPYPLYSKWFSEYAKIDPSVKFNYQSIGSGGGIKQITAQTVDFG
ASDKFLSDAELKAAPGKLLHIPTVMGAVVVTYNLPGAPKGLKLKAEDVADIFLGKISKWNDPRIADDNPGVKLPNSPIIV
VHRSDGSGTTSIFTDFLSGVNQEWAKKVGKGASVKWPIGLGGKGNEGVAGQIKTTANSIGYVELAYAFENKLPYATLKNK
SGSWVVPSIQTTSAAAAAAVKHMPADYRISLVNQPGKDAYPIVGFTWLLVYEKQKDAVKGKKLVEFLNWSLRNGQKMAAP
LLYAPLPENVKKMVEKTIKTVK

Specific function: Part of the ABC transporter complex pstSACB involved in phosphate import [H]

COG id: COG0226

COG function: function code P; ABC-type phosphate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pstS family [H]

Homologues:

Organism=Escherichia coli, GI2367271, Length=340, Percent_Identity=45, Blast_Score=275, Evalue=3e-75,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005673
- InterPro:   IPR006059 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 36497; Mature: 36497

Theoretical pI: Translated: 10.24; Mature: 10.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKALRKSLAILATLAVTAAAGQAGAETLINGAGATFPYPLYSKWFSEYAKIDPSVKFNY
CHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEEH
QSIGSGGGIKQITAQTVDFGASDKFLSDAELKAAPGKLLHIPTVMGAVVVTYNLPGAPKG
EECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEHHHHHEEEEEEEECCCCCCC
LKLKAEDVADIFLGKISKWNDPRIADDNPGVKLPNSPIIVVHRSDGSGTTSIFTDFLSGV
CEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCC
NQEWAKKVGKGASVKWPIGLGGKGNEGVAGQIKTTANSIGYVELAYAFENKLPYATLKNK
CHHHHHHHCCCCCEEECCCCCCCCCCCCCEEEEECCCCCCEEEEEEEHHCCCCEEEECCC
SGSWVVPSIQTTSAAAAAAVKHMPADYRISLVNQPGKDAYPIVGFTWLLVYEKQKDAVKG
CCCEEECCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEEEEEEEEECCHHHHHH
KKLVEFLNWSLRNGQKMAAPLLYAPLPENVKKMVEKTIKTVK
HHHHHHHHHHHCCCCCHHCCEEECCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLKALRKSLAILATLAVTAAAGQAGAETLINGAGATFPYPLYSKWFSEYAKIDPSVKFNY
CHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEEH
QSIGSGGGIKQITAQTVDFGASDKFLSDAELKAAPGKLLHIPTVMGAVVVTYNLPGAPKG
EECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEHHHHHEEEEEEEECCCCCCC
LKLKAEDVADIFLGKISKWNDPRIADDNPGVKLPNSPIIVVHRSDGSGTTSIFTDFLSGV
CEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCC
NQEWAKKVGKGASVKWPIGLGGKGNEGVAGQIKTTANSIGYVELAYAFENKLPYATLKNK
CHHHHHHHCCCCCEEECCCCCCCCCCCCCEEEEECCCCCCEEEEEEEHHCCCCEEEECCC
SGSWVVPSIQTTSAAAAAAVKHMPADYRISLVNQPGKDAYPIVGFTWLLVYEKQKDAVKG
CCCEEECCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEEEEEEEEECCHHHHHH
KKLVEFLNWSLRNGQKMAAPLLYAPLPENVKKMVEKTIKTVK
HHHHHHHHHHHCCCCCHHCCEEECCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; phosphate [Periplasm]; H2O [C]

Specific reaction: ATP + phosphate [Periplasm] + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]