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The map label for this gene is rlmN

Identifier: 78223881

GI number: 78223881

Start: 3032398

End: 3033438

Strand: Reverse

Name: rlmN

Synonym: Gmet_2685

Alternate gene names: 78223881

Gene position: 3033438-3032398 (Counterclockwise)

Preceding gene: 78223882

Following gene: 78223880

Centisome position: 75.88

GC content: 56.29

Gene sequence:

>1041_bases
ATGATGAACGGCAAAATTGATATTAAGAACCTGACCCTTGACGATCTCATTGCATTTCTTGCCGGTAAGGGGAAGGAGCG
CTATCGGGCGCGGCAGATATTTAAGTGGCTCTATCAGAAGGACGCCCGGAGTTTTGCCGAGATGACCGATCTGGCCAAGG
ATCTTCGGCGGGATCTGGAGGAAACAGCGGTCATCAGCGATCTTGAGCCCGAGGCGATGGAAGTATCCCGGGATGGTACG
CGGAAGTATCTGTTTCGCCTCGAGGACGGTAACACCGTCGAGTCAGTCCTCATCCCCGAAGAAGACCGCACTACCCTCTG
TATTTCGAGCCAGGTCGGTTGTGCCATGGCCTGCGAATTCTGTCTCACCGGCACGTTCCGGCTCACCCGGAACCTGACCG
CAGGCGAGATCGTTAACCAGATATGTGCGGTGCGAAGGGACGTACCGGTTCGCAATATCGTCTTCATGGGGATGGGAGAG
CCGCTGGCCAACCTTGATAATGTCGTGAAGGCACTCAAGATCATTCTTCACGATGACGGGCTCCAGTTCTCGACCCGACG
GGTCACGGTTTCCACATCGGGGCTTGTTCCGGAGATGGAGCGTTTGGGGCGCGAGGTAACGGTCAATCTGGCTGTCTCCC
TGAACGCTACCACCGACGAGGTGCGGGACCGGATCATGCCCGTGAACCGGCGTTATCCTCTCCGGTTGCTCCTGGACGCC
TGTCGAAGCTATCCCCTGCCGGGGCGCCGGAAGATTACCATCGAGTACGTGATGATCAAGGGGCTCAACGATTCTCTGGA
GGATGCCAAGCGGCTGGTGAAGCTCTTGAGCGACATCTCCTCCAAGATAAATCTCATTCCCTTCAACGAGCACGACGGTT
GCTCCTTCAAGTCTCCCGATCAGGGAGCAATCGATGTGTTTCACAGCTATCTCTTGAGCAAGCATTTTACGGTCATCACC
CGATCAAGCCGCGGCTCCGATATTTCCGCGGCGTGCGGCCAGCTCAAGGGTAAGCTGGACAAGAACGTTTCGGGCATCTA
A

Upstream 100 bases:

>100_bases
GATCCCTTACTTTTTCAGCCAGCTTGAACTCCTCTAGGTCATCCCAAGATTGCGAGGAAAAAGCCCTTCCCTTGAAGGGC
TTTTTTATATTCAATAAACC

Downstream 100 bases:

>100_bases
TTTCTGCAGCCTGGGAGGACTATGGCATGAGTGAGCAGGTTATCGGCGTCATTGGAGGAAGCGGTCTCTACGAGATGGAA
GGGCTGAGCGATGTCCGGAG

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MMNGKIDIKNLTLDDLIAFLAGKGKERYRARQIFKWLYQKDARSFAEMTDLAKDLRRDLEETAVISDLEPEAMEVSRDGT
RKYLFRLEDGNTVESVLIPEEDRTTLCISSQVGCAMACEFCLTGTFRLTRNLTAGEIVNQICAVRRDVPVRNIVFMGMGE
PLANLDNVVKALKIILHDDGLQFSTRRVTVSTSGLVPEMERLGREVTVNLAVSLNATTDEVRDRIMPVNRRYPLRLLLDA
CRSYPLPGRRKITIEYVMIKGLNDSLEDAKRLVKLLSDISSKINLIPFNEHDGCSFKSPDQGAIDVFHSYLLSKHFTVIT
RSSRGSDISAACGQLKGKLDKNVSGI

Sequences:

>Translated_346_residues
MMNGKIDIKNLTLDDLIAFLAGKGKERYRARQIFKWLYQKDARSFAEMTDLAKDLRRDLEETAVISDLEPEAMEVSRDGT
RKYLFRLEDGNTVESVLIPEEDRTTLCISSQVGCAMACEFCLTGTFRLTRNLTAGEIVNQICAVRRDVPVRNIVFMGMGE
PLANLDNVVKALKIILHDDGLQFSTRRVTVSTSGLVPEMERLGREVTVNLAVSLNATTDEVRDRIMPVNRRYPLRLLLDA
CRSYPLPGRRKITIEYVMIKGLNDSLEDAKRLVKLLSDISSKINLIPFNEHDGCSFKSPDQGAIDVFHSYLLSKHFTVIT
RSSRGSDISAACGQLKGKLDKNVSGI
>Mature_346_residues
MMNGKIDIKNLTLDDLIAFLAGKGKERYRARQIFKWLYQKDARSFAEMTDLAKDLRRDLEETAVISDLEPEAMEVSRDGT
RKYLFRLEDGNTVESVLIPEEDRTTLCISSQVGCAMACEFCLTGTFRLTRNLTAGEIVNQICAVRRDVPVRNIVFMGMGE
PLANLDNVVKALKIILHDDGLQFSTRRVTVSTSGLVPEMERLGREVTVNLAVSLNATTDEVRDRIMPVNRRYPLRLLLDA
CRSYPLPGRRKITIEYVMIKGLNDSLEDAKRLVKLLSDISSKINLIPFNEHDGCSFKSPDQGAIDVFHSYLLSKHFTVIT
RSSRGSDISAACGQLKGKLDKNVSGI

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=349, Percent_Identity=44.4126074498567, Blast_Score=285, Evalue=4e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_GEOMG (Q39S71)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_385628.1
- ProteinModelPortal:   Q39S71
- STRING:   Q39S71
- GeneID:   3740108
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_2685
- NMPDR:   fig|269799.3.peg.3199
- eggNOG:   COG0820
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- PhylomeDB:   Q39S71
- ProtClustDB:   PRK14463
- BioCyc:   GMET269799:GMET_2685-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 38838; Mature: 38838

Theoretical pI: Translated: 8.20; Mature: 8.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMNGKIDIKNLTLDDLIAFLAGKGKERYRARQIFKWLYQKDARSFAEMTDLAKDLRRDLE
CCCCEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ETAVISDLEPEAMEVSRDGTRKYLFRLEDGNTVESVLIPEEDRTTLCISSQVGCAMACEF
HHHHHCCCCCHHHHHCCCCCEEEEEEECCCCEEEEEECCCCCCCEEEEECCCCHHHHHHH
CLTGTFRLTRNLTAGEIVNQICAVRRDVPVRNIVFMGMGEPLANLDNVVKALKIILHDDG
HHCCHHHHHCCCCHHHHHHHHHHHHCCCCHHHEEEEECCCCHHHHHHHHHHHHHHHCCCC
LQFSTRRVTVSTSGLVPEMERLGREVTVNLAVSLNATTDEVRDRIMPVNRRYPLRLLLDA
CEEEEEEEEEECCCCCHHHHHCCCEEEEEEEEEECCCHHHHHHHHCCCCCCCHHHHHHHH
CRSYPLPGRRKITIEYVMIKGLNDSLEDAKRLVKLLSDISSKINLIPFNEHDGCSFKSPD
HHCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCC
QGAIDVFHSYLLSKHFTVITRSSRGSDISAACGQLKGKLDKNVSGI
CHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MMNGKIDIKNLTLDDLIAFLAGKGKERYRARQIFKWLYQKDARSFAEMTDLAKDLRRDLE
CCCCEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ETAVISDLEPEAMEVSRDGTRKYLFRLEDGNTVESVLIPEEDRTTLCISSQVGCAMACEF
HHHHHCCCCCHHHHHCCCCCEEEEEEECCCCEEEEEECCCCCCCEEEEECCCCHHHHHHH
CLTGTFRLTRNLTAGEIVNQICAVRRDVPVRNIVFMGMGEPLANLDNVVKALKIILHDDG
HHCCHHHHHCCCCHHHHHHHHHHHHCCCCHHHEEEEECCCCHHHHHHHHHHHHHHHCCCC
LQFSTRRVTVSTSGLVPEMERLGREVTVNLAVSLNATTDEVRDRIMPVNRRYPLRLLLDA
CEEEEEEEEEECCCCCHHHHHCCCEEEEEEEEEECCCHHHHHHHHCCCCCCCHHHHHHHH
CRSYPLPGRRKITIEYVMIKGLNDSLEDAKRLVKLLSDISSKINLIPFNEHDGCSFKSPD
HHCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCC
QGAIDVFHSYLLSKHFTVITRSSRGSDISAACGQLKGKLDKNVSGI
CHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA